Restriction map for
pFN21AE3132
(Circular) (Six-base) MAPSORT of: pFN21AE3132.seq Check: 8342 from: 1 to: 5713
pFN21AE3132 5713 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 3056 1937
Size: 435 684 4594
Fragments arranged by size:
4594 684 435
Acc65I G'GTAC_C
Cuts at: 2399 3034 2399
Size: 635 5078
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2261 3776 5160 5428 1632
Size: 488 141 1515 1384 268 1917
Fragments arranged by size:
1917 1515 1384 488 268 141
AflII C'TTAA_G
Cuts at: 820 1017 2150 2171 820
Size: 197 1133 21 4362
Fragments arranged by size:
4362 1133 197 21
AflIII A'CryG_T
Cuts at: 1203 4612 5589 1203
Size: 3409 977 1327
Fragments arranged by size:
3409 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2136 1184
Size: 952 4761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2022
Size: 32 5681
AlwNI CAG_nnn'CTG
Cuts at: 3456 5028 3456
Size: 1572 4141
ApaLI G'TGCA_C
Cuts at: 3770 4926 3770
Size: 1156 4557
ApoI r'AATT_y
Cuts at: 1493 2286 3022 3248 3400 5509 1493
Size: 793 736 226 152 2109 1697
Fragments arranged by size:
2109 1697 793 736 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 1870
Size: 73 1095 4545
Fragments arranged by size:
4545 1095 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2373 2406 2373
Size: 33 5680
BamHI G'GATC_C
Cuts at: 3043 3410 3043
Size: 367 5346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 3034 4489 618
Size: 325 217 627 612 635 1455 1842
Fragments arranged by size:
1842 1455 635 627 612 325 217
BanII G_rGCy'C
Cuts at: 1391 2986 3032 1391
Size: 1595 46 4072
Fragments arranged by size:
4072 1595 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2538 2572 3904 3938 1903
Size: 34 50 34 517 34 1332 34 3678
Fragments arranged by size:
3678 1332 517 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3605 4815 3605
Size: 1210 4503
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4324 136
Size: 107 122 71 631 605 2652 1525
Fragments arranged by size:
2652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 3130 3130
Size: 5713
Bme1580I G_kGCm'C
Cuts at: 1790 3774 4930 1790
Size: 1984 1156 2573
Fragments arranged by size:
2573 1984 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5713
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3444 4402 442
Size: 416 53 83 399 24 2027 958 1753
Fragments arranged by size:
2027 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2838 4373 1239
Size: 890 709 1535 2579
Fragments arranged by size:
2579 1535 890 709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2730 3838 4703 5001 2730
Size: 1108 865 298 3442
Fragments arranged by size:
3442 1108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 493
Size: 711 2300 2702
Fragments arranged by size:
2702 2300 711
BsaBI GATnn'nnATC
Cuts at: 5707 5707
Size: 5713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 3902 4537 275
Size: 53 83 186 936 1987 382 635 1451
Fragments arranged by size:
1987 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5488
Fragments arranged by size:
5488 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2787 1186
Size: 1601 4112
BseYI C'CCAG_C
Cuts at: 4916 5412 4916
Size: 496 5217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 2318 1163
Size: 573 406 176 4558
Fragments arranged by size:
4558 573 406 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3924 4952 1540
Size: 275 186 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3774 3859 4930 3774
Size: 85 1071 4557
Fragments arranged by size:
4557 1071 85
BsmI GAATG_Cn'
Cuts at: 3224 3317 3224
Size: 93 5620
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2424 2069
Size: 355 5358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2986 3032 3774 3859 4930 1391
Size: 399 1196 46 742 85 1071 2174
Fragments arranged by size:
2174 1196 1071 742 399 85 46
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 2049 3602 5332 2049
Size: 1553 1730 2430
Fragments arranged by size:
2430 1730 1553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2728 3068 844
Size: 1884 340 3489
Fragments arranged by size:
3489 1884 340
BsrBI CCG'CTC
Cuts at: 1394 1823 3600 1394
Size: 429 1777 3507
Fragments arranged by size:
3507 1777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2757 4208 4382 65
Size: 1183 1509 1451 174 1396
Fragments arranged by size:
1509 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4357 4544 1700
Size: 110 2547 187 2869
Fragments arranged by size:
2869 2547 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 3773 4785 3773
Size: 1012 4701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5713
BstBI TT'CG_AA
Cuts at: 2548 4517 2548
Size: 1969 3744
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5713
BstYI r'GATC_y
Cuts at: 1972 2542 2895 3043 3410 3795 3812 5264 5350
Size: 570 353 148 367 385 17 1452 86
Cuts at: 5350 5362 5708 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 570 385 367 353 346 148
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2034 513
Size: 552 969 4192
Fragments arranged by size:
4192 969 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4974
Fragments arranged by size:
4974 683 56
BtsI GCAGTG_nn'
Cuts at: 2330 3229 4023 4043 2330
Size: 899 794 20 4000
Fragments arranged by size:
4000 899 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5678
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3160 4049 5678
Size: 54 1425 50 275 18 1330 889 1629
Cuts at: 5678 8
Size: 43
Fragments arranged by size:
1629 1425 1330 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3160 1537
Size: 275 1348 4090
Fragments arranged by size:
4090 1348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2202 3643 1353
Size: 743 106 1441 3423
Fragments arranged by size:
3423 1441 743 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4300 4674 4820 387
Size: 1064 138 2711 374 146 1280
Fragments arranged by size:
2711 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2129 2261 2838 3776 4373 5160
Size: 393 488 9 132 577 938 597 787
Cuts at: 5160 5428 1239
Size: 268 1524
Fragments arranged by size:
1524 938 787 597 577 488 393 268
132 9
EcoRI G'AATT_C
Cuts at: 3022 3400 3022
Size: 378 5335
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 4860 1678
Size: 3182 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2671 2703 4354 4386 4428
Size: 32 135 32 1099 32 1651 32 42
Cuts at: 4428 4460 1373
Size: 32 2626
Fragments arranged by size:
2626 1651 1099 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3303 669
Size: 988 281 1119 246 3079
Fragments arranged by size:
3079 1119 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 2403 3038 2403
Size: 635 5078
MfeI C'AATT_G
Cuts at: 3312 3312
Size: 5713
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2599 4827 5011 1305
Size: 1294 2228 184 2007
Fragments arranged by size:
2228 2007 1294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3891
Fragments arranged by size:
3891 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2916 3671 4030 4189 518
Size: 648 1417 333 755 359 159 2042
Fragments arranged by size:
2042 1417 755 648 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 3129 3806 4954 5199 1606
Size: 1523 677 1148 245 2120
Fragments arranged by size:
2120 1523 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5161
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 3071 4616 1132
Size: 97 1842 1545 2229
Fragments arranged by size:
2229 1842 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5713
PciI A'CATG_T
Cuts at: 4612 4612
Size: 5713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5713
PfoI T'CCnGG_A
Cuts at: 2815 2815
Size: 5713
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2642 1613
Size: 1029 4684
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 3065 830
Size: 2235 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2373 3061 4196 4877 5068 826
Size: 219 1328 688 1135 681 191 1471
Fragments arranged by size:
1471 1328 1135 688 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2171 2745 3817 4718 4980
Size: 197 926 207 21 574 1072 901 262
Cuts at: 4980 820
Size: 1553
Fragments arranged by size:
1553 1072 926 901 574 262 207 197
21
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5713
StyI C'CwwG_G
Cuts at: 513 1065 2633 3152 513
Size: 552 1568 519 3074
Fragments arranged by size:
3074 1568 552 519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3740 3757 3910 4095 4540 325
Size: 1255 2160 17 153 185 445 1498
Fragments arranged by size:
2160 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 5671 96
Size: 274 80 33 51 1443 1982 1712 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 3842 3842
Size: 5713
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI