Restriction map for
pFN21AE3130
(Circular) (Six-base) MAPSORT of: pFN21AE3130.seq Check: 136 from: 1 to: 5674
pFN21AE3130 5674 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2714 3017 1937
Size: 777 303 4594
Fragments arranged by size:
4594 777 303
Acc65I G'GTAC_C
Cuts at: 2082 2995 2082
Size: 913 4761
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 3737 5121 5389 1632
Size: 843 1262 1384 268 1917
Fragments arranged by size:
1917 1384 1262 843 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2140 3417 4989 2140
Size: 1277 1572 2825
Fragments arranged by size:
2825 1572 1277
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2181 2726 2983 3209 3361 5470 1493
Size: 688 545 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 688 545 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2999 1870
Size: 73 70 986 4545
Fragments arranged by size:
4545 986 73 70
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2082 2995 4450 618
Size: 325 217 627 251 44 913 1455 1842
Fragments arranged by size:
1842 1455 913 627 325 251 217 44
BanII G_rGCy'C
Cuts at: 1391 2013 2794 2993 1391
Size: 622 781 199 4072
Fragments arranged by size:
4072 781 622 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2676 928
Size: 971 777 3926
Fragments arranged by size:
3926 971 777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BfrBI ATG'CAT
Cuts at: 2906 2906
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2703 4334 1239
Size: 1076 388 1631 2579
Fragments arranged by size:
2579 1631 1076 388
Bpu10I CC'TnA_GC
Cuts at: 2914 2914
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2650 2801 3799 4664 4962 2387
Size: 263 151 998 865 298 3099
Fragments arranged by size:
3099 998 865 298 263 151
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2239 2832 1186
Size: 1053 593 4028
Fragments arranged by size:
4028 1053 593
BseYI C'CCAG_C
Cuts at: 2077 4877 5373 2077
Size: 2800 496 2378
Fragments arranged by size:
2800 2378 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 1163
Size: 573 338 4763
Fragments arranged by size:
4763 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2614 2794 3735 3820 4891 2614
Size: 180 941 85 1071 3397
Fragments arranged by size:
3397 1071 941 180 85
BsmI GAATG_Cn'
Cuts at: 2203 3185 3278 2203
Size: 982 93 4599
Fragments arranged by size:
4599 982 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2398 2398
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2614 2794 2993 3735 3820 4891
Size: 399 223 601 180 199 742 85 1071
Cuts at: 4891 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 601 399 223 199 180
85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 3029 844
Size: 1202 983 3489
Fragments arranged by size:
3489 1202 983
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 4318 4505 1700
Size: 110 239 2269 187 2869
Fragments arranged by size:
2869 2269 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 2491
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669
Size: 1032 367 385 17 1452 86 12 346
Cuts at: 5669 1972
Size: 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2715 2715
Size: 5674
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2135 487
Size: 683 56 909 4026
Fragments arranged by size:
4026 909 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2560 3121
Size: 54 1425 50 275 18 420 310 561
Cuts at: 3121 4010 5639 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 561 420 310 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2124 2216 3604 1353
Size: 756 15 92 1388 3423
Fragments arranged by size:
3423 1388 756 92 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2792 2792
Size: 5674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2475 2703 3737 4334 5121 5389
Size: 393 683 160 228 1034 597 787 268
Cuts at: 5389 1239
Size: 1524
Fragments arranged by size:
1524 1034 787 683 597 393 268 228
160
EcoNI CCTnn'n_nnAGG
Cuts at: 2700 2700
Size: 5674
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
EcoRV GAT'ATC
Cuts at: 2388 2388
Size: 5674
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2134 2166 4315 4347 4389
Size: 32 135 32 562 32 2149 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 2149 562 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 3018 3264 669
Size: 988 281 409 671 246 3079
Fragments arranged by size:
3079 988 671 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 2347 3264 2347
Size: 917 4757
KpnI G_GTAC'C
Cuts at: 2086 2999 2086
Size: 913 4761
MfeI C'AATT_G
Cuts at: 2300 3273 2300
Size: 973 4701
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 4788 4972 1305
Size: 933 2550 184 2007
Fragments arranged by size:
2550 2007 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2562 10
Size: 54 1425 343 420 310 3122
Fragments arranged by size:
3122 1425 420 343 310 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2907 3632 3991 4150 518
Size: 648 1741 725 359 159 2042
Fragments arranged by size:
2042 1741 725 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2645 2762 3090 3767 4915 5160 1606
Size: 531 508 117 328 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 508 328 245 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 2490 2908 387
Size: 2103 418 3153
Fragments arranged by size:
3153 2103 418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NsiI A_TGCA'T
Cuts at: 2908 2908
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2906 3032 4577 1132
Size: 97 1677 126 1545 2229
Fragments arranged by size:
2229 1677 1545 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 1108
Size: 1450 4224
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 2928 3244 2928
Size: 316 5358
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2137 2762 2137
Size: 625 5049
SacI G_AGCT'C
Cuts at: 2794 2794
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SapI GCTCTTCn'nnn_
Cuts at: 2109 2124 2109
Size: 15 5659
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 2738 3922 5634 2738
Size: 1184 1712 2778
Fragments arranged by size:
2778 1712 1184
SexAI A'CCwGG_T
Cuts at: 2085 2085
Size: 5674
SfcI C'TryA_G
Cuts at: 826 1045 3022 4157 4838 5029 826
Size: 219 1977 1135 681 191 1471
Fragments arranged by size:
1977 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 2015 3001 1872
Size: 143 986 4545
Fragments arranged by size:
4545 986 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 2629 2780 3778 4679 4941
Size: 197 926 423 263 151 998 901 262
Cuts at: 4941 820
Size: 1553
Fragments arranged by size:
1553 998 926 901 423 263 262 197
151
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2517 2867 3598 5
Size: 47 2465 350 731 2081
Fragments arranged by size:
2465 2081 731 350 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 3113 513
Size: 552 939 1109 3074
Fragments arranged by size:
3074 1109 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 3701 3718 3871 4056 4501 325
Size: 1255 546 1575 17 153 185 445 1498
Fragments arranged by size:
1575 1498 1255 546 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2736 3920 5632
Size: 274 80 33 51 1443 759 1184 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1712 1443 1184 759 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2013 2999 1870
Size: 143 986 4545
Fragments arranged by size:
4545 986 143
XmnI GAAnn'nnTTC
Cuts at: 2673 2727 3803 2673
Size: 54 1076 4544
Fragments arranged by size:
4544 1076 54
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI Bsu36I DraIII EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI