Restriction map for
pFN21AE3129
(Circular) (Six-base) MAPSORT of: pFN21AE3129.seq Check: 4494 from: 1 to: 5671
pFN21AE3129 5671 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2213 3481 278
Size: 53 83 186 936 677 1268 2468
Fragments arranged by size:
2468 1268 936 677 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 3734 5118 5386 1632
Size: 1107 995 1384 268 1917
Fragments arranged by size:
1917 1384 1107 995 268
AfeI AGC'GCT
Cuts at: 2644 2644
Size: 5671
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 5639
AlwNI CAG_nnn'CTG
Cuts at: 2327 2543 2654 2750 3414 4986 2327
Size: 216 111 96 664 1572 3012
Fragments arranged by size:
3012 1572 664 216 111 96
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2291 2386 2980 3206 3358 5467 1493
Size: 798 95 594 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 594 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 2560 3001 3368 2560
Size: 441 367 4863
Fragments arranged by size:
4863 441 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2992 4447 618
Size: 325 217 627 381 824 1455 1842
Fragments arranged by size:
1842 1455 824 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2827 2990 1391
Size: 681 755 163 4072
Fragments arranged by size:
4072 755 681 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2874 928
Size: 971 975 3725
Fragments arranged by size:
3725 975 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2785 3563 4773 2785
Size: 778 1210 3683
Fragments arranged by size:
3683 1210 778
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4282 136
Size: 107 122 71 631 605 337 2273 1525
Fragments arranged by size:
2273 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 3402 4360
Size: 416 53 83 399 24 1143 842 958
Cuts at: 4360 442
Size: 1753
Fragments arranged by size:
1753 1143 958 842 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2400 2457 4331 1239
Size: 900 261 57 1874 2579
Fragments arranged by size:
2579 1874 900 261 57
Bpu10I CC'TnA_GC
Cuts at: 2751 2751
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2210 3478 3860
Size: 53 83 186 936 479 198 1268 382
Cuts at: 3860 4495 275
Size: 635 1451
Fragments arranged by size:
1451 1268 936 635 479 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 4800
BseYI C'CCAG_C
Cuts at: 2654 4874 5370 2654
Size: 2220 496 2955
Fragments arranged by size:
2955 2220 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2676 2827 3732 3817 4888 2676
Size: 151 905 85 1071 3459
Fragments arranged by size:
3459 1071 905 151 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2676 2827 2990 3732 3817 4888
Size: 399 282 604 151 163 742 85 1071
Cuts at: 4888 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 604 399 282 163 151
85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 2254 2547 2605 2892 4166 4340
Size: 1183 963 43 293 58 287 1274 174
Cuts at: 4340 65
Size: 1396
Fragments arranged by size:
1396 1274 1183 963 293 287 174 58
43
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 2449
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2407 2560 2882 3001 3368 3753 3770 5222
Size: 435 153 322 119 367 385 17 1452
Cuts at: 5222 5308 5320 5666 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 435 385 367 346 322 153
119 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2710 2710
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 2214 2796 513
Size: 552 939 127 83 582 3388
Fragments arranged by size:
3388 939 582 552 127 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2039 3187 3981 4001 2039
Size: 1148 794 20 3709
Fragments arranged by size:
3709 1148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3118 4007 5636
Size: 54 1425 50 275 18 1288 889 1629
Cuts at: 5636 8
Size: 43
Fragments arranged by size:
1629 1425 1288 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2825 2825
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2400 2457 2739 3734 4331 5118
Size: 393 507 261 57 282 995 597 787
Cuts at: 5118 5386 1239
Size: 268 1524
Fragments arranged by size:
1524 995 787 597 507 393 282 268
261 57
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2646 4818 1678
Size: 968 2172 2531
Fragments arranged by size:
2531 2172 968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 2430 2462 4312
Size: 32 135 32 540 32 286 32 1850
Cuts at: 4312 4344 4386 4418 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1850 540 286 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3849
Fragments arranged by size:
3849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3988 4147 518
Size: 648 2463 359 159 2042
Fragments arranged by size:
2463 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2131 2214 513
Size: 552 939 127 83 3970
Fragments arranged by size:
3970 939 552 127 83
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2875 3029 4574 1132
Size: 97 1646 154 1545 2229
Fragments arranged by size:
2229 1646 1545 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2117 1108
Size: 1009 4662
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2650 2920 3023 830
Size: 1820 270 103 3478
Fragments arranged by size:
3478 1820 270 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SacI G_AGCT'C
Cuts at: 2827 2827
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SexAI A'CCwGG_T
Cuts at: 2085 2085
Size: 5671
SfcI C'TryA_G
Cuts at: 826 1045 2286 2319 2646 2916 3019 4154 4835
Size: 219 1241 33 327 270 103 1135 681
Cuts at: 4835 5026 826
Size: 191 1471
Fragments arranged by size:
1471 1241 1135 681 327 270 219 191
103 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2131 2214 2862 3110 513
Size: 552 939 127 83 648 248 3074
Fragments arranged by size:
3074 939 648 552 248 127 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 3698 3715 3868 4053 4498 325
Size: 1255 516 1602 17 153 185 445 1498
Fragments arranged by size:
1602 1498 1255 516 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2715 2747 5631 5663 2715
Size: 32 2884 32 2723
Fragments arranged by size:
2884 2723 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2087 1316
Size: 771 4900
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 2554
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2329 3800 2329
Size: 1471 4200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2211 3479 276
Size: 53 83 186 936 677 1268 2468
Fragments arranged by size:
2468 1268 936 677 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI