Restriction map for
pFN21AE3128
(Circular) (Six-base) MAPSORT of: pFN21AE3128.seq Check: 3105 from: 1 to: 5659
pFN21AE3128 5659 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2163 3002 1937
Size: 226 839 4594
Fragments arranged by size:
4594 839 226
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3722 5106 5374 1632
Size: 2090 1384 268 1917
Fragments arranged by size:
2090 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2047 3402 4974 2047
Size: 1355 1572 2732
Fragments arranged by size:
2732 1572 1355
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2567 2968 3194 3346 5455 1493
Size: 1074 401 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 401 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 2980 4435 618
Size: 325 217 627 897 296 1455 1842
Fragments arranged by size:
1842 1455 897 627 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2451 2978 1391
Size: 1060 527 4072
Fragments arranged by size:
4072 1060 527
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2450 2484 3850 3884 1903
Size: 34 50 34 429 34 1366 34 3678
Fragments arranged by size:
3678 1366 429 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2223 1548
Size: 675 4984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2804 4270 136
Size: 107 122 71 631 605 1132 1466 1525
Fragments arranged by size:
1525 1466 1132 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2320 3720 4876 1790
Size: 530 1400 1156 2573
Fragments arranged by size:
2573 1400 1156 530
BmgBI CAC'GTC
Cuts at: 1318 2635 1318
Size: 1317 4342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2742 4319 1239
Size: 860 643 1577 2579
Fragments arranged by size:
2579 1577 860 643
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 4322 882
Size: 617 615 2208 2219
Fragments arranged by size:
2219 2208 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2613 4092 4490 4764 4911 5322 2613
Size: 1479 398 274 147 411 2950
Fragments arranged by size:
2950 1479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 1186
Size: 1018 4641
BseYI C'CCAG_C
Cuts at: 2052 2083 2360 4862 5358 2052
Size: 31 277 2502 496 2353
Fragments arranged by size:
2502 2353 496 277 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2839 1163
Size: 573 1103 3983
Fragments arranged by size:
3983 1103 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 3109 3870 4898 1540
Size: 275 186 67 1041 761 1028 2301
Fragments arranged by size:
2301 1041 1028 761 275 186 67
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2320 2451 2978 3720 3805 4876 1391
Size: 399 530 131 527 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 527 399 131 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2220 3548 5278 2220
Size: 1328 1730 2601
Fragments arranged by size:
2601 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 3014 844
Size: 1553 617 3489
Fragments arranged by size:
3489 1553 617
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 3546 1394
Size: 429 355 1368 3507
Fragments arranged by size:
3507 1368 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 4154 4328 65
Size: 1183 1230 1676 174 1396
Fragments arranged by size:
1676 1396 1230 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 2657 3719 4731 2657
Size: 1062 1012 3585
Fragments arranged by size:
3585 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2691 794
Size: 1897 3762
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2659 2890 2659
Size: 231 5428
BstYI r'GATC_y
Cuts at: 1972 2757 2989 3356 3741 3758 5210 5296 5308
Size: 785 232 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 785 385 367 346 232 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2382 2752 513
Size: 552 1317 370 3420
Fragments arranged by size:
3420 1317 552 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2851 3175 3969 3989 2468
Size: 383 324 794 20 4138
Fragments arranged by size:
4138 794 383 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2319 2354 529
Size: 35 1755 35 3834
Fragments arranged by size:
3834 1755 35 35
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2618 4666 809
Size: 507 1302 2048 1802
Fragments arranged by size:
2048 1802 1302 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2340 2649
Size: 54 1425 50 275 18 231 279 309
Cuts at: 2649 2892 3106 3995 5624 8
Size: 243 214 889 1629 43
Fragments arranged by size:
1629 1425 889 309 279 275 243 231
214 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3589 1353
Size: 2236 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2381 4246 4620 4766 387
Size: 1064 138 792 1865 374 146 1280
Fragments arranged by size:
1865 1280 1064 792 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2742 3722 4319 5106 5374 1239
Size: 393 467 643 980 597 787 268 1524
Fragments arranged by size:
1524 980 787 643 597 467 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2517 2517
Size: 5659
EcoO109I rG'GnC_Cy
Cuts at: 2044 2044
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 2544 3258 2544
Size: 714 4945
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 2651 10
Size: 54 1425 343 510 309 3018
Fragments arranged by size:
3018 1425 510 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2657 2732 2882 3617 3976 4135
Size: 648 1215 276 75 150 735 359 159
Cuts at: 4135 518
Size: 2042
Fragments arranged by size:
2042 1215 735 648 359 276 159 150
75
MspA1I CmG'CkG
Cuts at: 1606 2028 2720 3075 3752 4900 5145 1606
Size: 422 692 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 422 355 245
NcoI C'CATG_G
Cuts at: 513 1065 2382 2752 513
Size: 552 1317 370 3420
Fragments arranged by size:
3420 1317 552 370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2396 3017 4562 1132
Size: 97 1167 621 1545 2229
Fragments arranged by size:
2229 1545 1167 621 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 1108
Size: 951 4708
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2044
Size: 5659
PshAI GACnn'nnGTC
Cuts at: 1613 2310 1613
Size: 697 4962
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2463 3011 830
Size: 1633 548 3478
Fragments arranged by size:
3478 1633 548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2720 2720
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2459 3007 4142 4823 5014 826
Size: 219 1414 548 1135 681 191 1471
Fragments arranged by size:
1471 1414 1135 681 548 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2396 3017 1132
Size: 1264 621 3774
Fragments arranged by size:
3774 1264 621
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2382 2752 3098 513
Size: 552 1317 370 346 3074
Fragments arranged by size:
3074 1317 552 370 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2105 3788 2105
Size: 1683 3976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI