Restriction map for
pFN21AE3124
(Circular) (Six-base) MAPSORT of: pFN21AE3124.seq Check: 3261 from: 1 to: 5644
pFN21AE3124 5644 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2697 3707 5091 5359 1632
Size: 563 502 1010 1384 268 1917
Fragments arranged by size:
1917 1384 1010 563 502 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2219 1184
Size: 1035 4609
AlwNI CAG_nnn'CTG
Cuts at: 2054 2152 3387 4959 2054
Size: 98 1235 1572 2739
Fragments arranged by size:
2739 1572 1235 98
ApaI G_GGCC'C
Cuts at: 2932 2932
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2300 2969 1870
Size: 73 357 669 4545
Fragments arranged by size:
4545 669 357 73
BamHI G'GATC_C
Cuts at: 2226 2901 2974 3341 2226
Size: 675 73 367 4529
Fragments arranged by size:
4529 675 367 73
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2290 2965 4420 618
Size: 325 217 627 257 246 675 1455 1842
Fragments arranged by size:
1842 1455 675 627 325 257 246 217
BanII G_rGCy'C
Cuts at: 1391 2039 2090 2128 2932 2963 1391
Size: 648 51 38 804 31 4072
Fragments arranged by size:
4072 804 648 51 38 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 4762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 2038 3061 2038
Size: 1023 4621
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2932 3705 4861 1790
Size: 294 848 773 1156 2573
Fragments arranged by size:
2573 1156 848 773 294
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2333 1056
Size: 1277 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 4304 1239
Size: 1601 1464 2579
Fragments arranged by size:
2579 1601 1464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 4307 882
Size: 617 1254 1554 2219
Fragments arranged by size:
2219 1554 1254 617
BsaAI yAC'GTr
Cuts at: 493 1204 2104 3435 493
Size: 711 900 1331 2702
Fragments arranged by size:
2702 1331 900 711
BsaBI GATnn'nnATC
Cuts at: 2906 5638 2906
Size: 2732 2912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 1178
Size: 30 165 30 610 30 4779
Fragments arranged by size:
4779 610 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2771 2885 1186
Size: 1150 435 114 3945
Fragments arranged by size:
3945 1150 435 114
BseYI C'CCAG_C
Cuts at: 2090 4847 5343 2090
Size: 2757 496 2391
Fragments arranged by size:
2757 2391 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2039 2208 3705 3790 4861 2039
Size: 169 1497 85 1071 2822
Fragments arranged by size:
2822 1497 1071 169 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2274 2274
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2084 2090 2128 2208 2932 2963
Size: 399 249 45 6 38 80 724 31
Cuts at: 2963 3705 3790 4861 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 724 399 249 85 80
45 38 31 6
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2875 2881 3531 1394
Size: 429 1052 6 650 3507
Fragments arranged by size:
3507 1052 650 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 2083 3704 4716 2083
Size: 1621 1012 3011
Fragments arranged by size:
3011 1621 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2226 2901 2974 3341 3726 3743 5195 5281
Size: 254 675 73 367 385 17 1452 86
Cuts at: 5281 5293 5639 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 675 385 367 346 254 86
73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2794 3091 3980
Size: 54 1425 50 275 18 964 297 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 964 889 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2364 3574 1353
Size: 651 360 1210 3423
Fragments arranged by size:
3423 1210 651 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2037 2037
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2697 2840 3707 4304 5091 5359
Size: 393 563 502 143 867 597 787 268
Cuts at: 5359 1239
Size: 1524
Fragments arranged by size:
1524 867 787 597 563 502 393 268
143
EcoNI CCTnn'n_nnAGG
Cuts at: 2861 2939 2861
Size: 78 5566
EcoO109I rG'GnC_Cy
Cuts at: 2422 2602 2801 2422
Size: 180 199 5265
Fragments arranged by size:
5265 199 180
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2848 2917 4791 1678
Size: 1170 69 1874 2531
Fragments arranged by size:
2531 1874 1170 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 4285 4317 4359
Size: 32 135 32 992 32 1689 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 992 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 3602 3961 4120 518
Size: 648 1035 1401 359 159 2042
Fragments arranged by size:
2042 1401 1035 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2051 2190 2241 3060 3737 4885 5130
Size: 436 9 139 51 819 677 1148 245
Cuts at: 5130 1606
Size: 2120
Fragments arranged by size:
2120 1148 819 677 436 245 139 51
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 2329 1052
Size: 1277 4367
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 2857 1108
Size: 1254 495 3895
Fragments arranged by size:
3895 1254 495
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2602 2801 2422
Size: 180 199 5265
Fragments arranged by size:
5265 199 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2928 2928
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2039 2039
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 2345 3892 5604 2345
Size: 1547 1712 2385
Fragments arranged by size:
2385 1712 1547
SexAI A'CCwGG_T
Cuts at: 2940 2940
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2992 4127 4808 4999 826
Size: 219 1947 1135 681 191 1471
Fragments arranged by size:
1947 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2361 1517
Size: 844 4800
StyI C'CwwG_G
Cuts at: 513 1065 2004 2356 2442 2724 3083 513
Size: 552 939 352 86 282 359 3074
Fragments arranged by size:
3074 939 552 359 352 282 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 3671 3688 3841 4026 4471 325
Size: 1255 528 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 528 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 3890 5602
Size: 274 80 33 51 1443 366 1547 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1547 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 5604 5636 2013
Size: 32 3559 32 2021
Fragments arranged by size:
3559 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI