Restriction map for
pFN21AE3122
(Circular) (Six-base) MAPSORT of: pFN21AE3122.seq Check: 2722 from: 1 to: 5629
pFN21AE3122 5629 bp
With 182 enzymes: SgfI *
March 28, 2012 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2313 2319 2481 2313
Size: 6 162 5461
Fragments arranged by size:
5461 162 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 3692 5076 5344 1632
Size: 1184 876 1384 268 1917
Fragments arranged by size:
1917 1384 1184 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 2876 1166
Size: 1710 3919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaI G_GGCC'C
Cuts at: 2625 2625
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 2125 2322 2336 2348 2618 2624
Size: 73 66 116 197 14 12 270 6
Cuts at: 2624 2737 2954 1870
Size: 113 217 4545
Fragments arranged by size:
4545 270 217 197 116 113 73 66
14 12 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2299 2332 2299
Size: 33 5596
BamHI G'GATC_C
Cuts at: 2291 2959 3326 2291
Size: 668 367 4594
Fragments arranged by size:
4594 668 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2458 2577 2809 2950
Size: 325 217 627 300 371 119 232 141
Cuts at: 2950 4405 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 371 325 300 232 217
141 119
BanII G_rGCy'C
Cuts at: 1391 2346 2374 2625 2948 1391
Size: 955 28 251 323 4072
Fragments arranged by size:
4072 955 323 251 28
BbeI G_GCGC'C
Cuts at: 2091 2462 2581 2091
Size: 371 119 5139
Fragments arranged by size:
5139 371 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2157 2673 928
Size: 971 258 516 3884
Fragments arranged by size:
3884 971 516 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2505 2539 3820 3854 1903
Size: 34 50 34 484 34 1281 34 3678
Fragments arranged by size:
3678 1281 484 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3521 4731 2259
Size: 1262 1210 3157
Fragments arranged by size:
3157 1262 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 2438 3046 2438
Size: 608 5021
Bme1580I G_kGCm'C
Cuts at: 1790 2625 2738 2832 3690 4846 1790
Size: 835 113 94 858 1156 2573
Fragments arranged by size:
2573 1156 858 835 113 94
BmgBI CAC'GTC
Cuts at: 1318 2413 1318
Size: 1095 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2564 2870 4289 1239
Size: 901 424 306 1419 2579
Fragments arranged by size:
2579 1419 901 424 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2396 2431 2459
Size: 53 83 186 936 555 308 35 28
Cuts at: 2459 2578 3436 3818 4453 275
Size: 119 858 382 635 1451
Fragments arranged by size:
1451 936 858 635 555 382 308 186
119 83 53 35 28
BsaWI w'CCGG_w
Cuts at: 2262 4062 4460 4734 4881 5292 2262
Size: 1800 398 274 147 411 2599
Fragments arranged by size:
2599 1800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5629
BseYI C'CCAG_C
Cuts at: 2220 2287 2538 2676 4832 5328 2220
Size: 67 251 138 2156 496 2521
Fragments arranged by size:
2521 2156 496 251 138 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 2453 2627 2771 1163
Size: 573 555 162 174 144 4021
Fragments arranged by size:
4021 573 555 174 162 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2561 3079 3840 4868 1540
Size: 275 186 560 518 761 1028 2301
Fragments arranged by size:
2301 1028 761 560 518 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2856 3690 3775 4846 2067
Size: 789 834 85 1071 2850
Fragments arranged by size:
2850 1071 834 789 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2346 2374 2625 2738 2832 2856
Size: 399 277 279 28 251 113 94 24
Cuts at: 2856 2948 3690 3775 4846 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 277 251 113
94 92 85 28 24
BspEI T'CCGG_A
Cuts at: 2262 5292 2262
Size: 3030 2599
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2313 2319 2481 2984 844
Size: 1355 114 6 162 503 3489
Fragments arranged by size:
3489 1355 503 162 114 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 3516 1394
Size: 429 794 899 3507
Fragments arranged by size:
3507 899 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 2594 1925
Size: 669 4960
BssSI C'ACGA_G
Cuts at: 2068 3689 4701 2068
Size: 1621 1012 2996
Fragments arranged by size:
2996 1621 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 2231 4433 2231
Size: 2202 3427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2291 2959 3326 3711 3728 5180 5266 5278
Size: 319 668 367 385 17 1452 86 12
Cuts at: 5278 5624 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 319 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2699 2847 513
Size: 552 1634 148 3295
Fragments arranged by size:
3295 1634 552 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3145 3939 3959 2920
Size: 225 794 20 4590
Fragments arranged by size:
4590 794 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2299 2558
Size: 54 1425 50 275 18 270 199 259
Cuts at: 2558 3076 3965 5594 8
Size: 518 889 1629 43
Fragments arranged by size:
1629 1425 889 518 275 270 259 199
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2558 3076 1537
Size: 275 746 518 4090
Fragments arranged by size:
4090 746 518 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3559 1353
Size: 2206 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2564 2816 2870 3692 4289 5076
Size: 393 508 424 252 54 822 597 787
Cuts at: 5076 5344 1239
Size: 268 1524
Fragments arranged by size:
1524 822 787 597 508 424 393 268
252 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 5629
EcoO109I rG'GnC_Cy
Cuts at: 2129 2188 2129
Size: 59 5570
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2091 2462 2581 2819 4776 1678
Size: 413 371 119 238 1957 2531
Fragments arranged by size:
2531 1957 413 371 238 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 4270 4302 4344
Size: 32 135 32 835 32 1831 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 1831 835 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KasI G'GCGC_C
Cuts at: 2087 2458 2577 2087
Size: 371 119 5139
Fragments arranged by size:
5139 371 119
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 4743 4927 1305
Size: 1176 2262 184 2007
Fragments arranged by size:
2262 2007 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3587 3946 4105 518
Size: 648 1710 711 359 159 2042
Fragments arranged by size:
2042 1710 711 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2542 3045 3722 4870 5115 1606
Size: 936 503 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 677 503 245
NarI GG'CG_CC
Cuts at: 2088 2459 2578 2088
Size: 371 119 5139
Fragments arranged by size:
5139 371 119
NcoI C'CATG_G
Cuts at: 513 1065 2847 513
Size: 552 1782 3295
Fragments arranged by size:
3295 1782 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 2558 3076 2558
Size: 518 5111
NspI r_CATG'y
Cuts at: 1132 1229 2764 2987 4532 1132
Size: 97 1535 223 1545 2229
Fragments arranged by size:
2229 1545 1535 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 1108
Size: 1404 4225
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 2188 2129
Size: 59 5570
PshAI GACnn'nnGTC
Cuts at: 1613 2610 1613
Size: 997 4632
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2621 2621
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2260 2981 830
Size: 1430 721 3478
Fragments arranged by size:
3478 1430 721
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2542 2542
Size: 5629
RsrII CG'GwC_CG
Cuts at: 2650 2650
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SanDI GG'GwC_CC
Cuts at: 2129 2129
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2256 2977 4112 4793 4984 826
Size: 219 1211 721 1135 681 191 1471
Fragments arranged by size:
1471 1211 1135 721 681 219 191
SfoI GGC'GCC
Cuts at: 2089 2460 2579 2089
Size: 371 119 5139
Fragments arranged by size:
5139 371 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2011 2127 2626 2956 1872
Size: 139 116 499 330 4545
Fragments arranged by size:
4545 499 330 139 116
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SrfI GCCC'GGGC
Cuts at: 2011 2626 2011
Size: 615 5014
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2354 1517
Size: 837 4792
StyI C'CwwG_G
Cuts at: 513 1065 2847 3068 513
Size: 552 1782 221 3074
Fragments arranged by size:
3074 1782 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2677 2882 3656 3673 3826 4011 4456
Size: 1255 1097 205 774 17 153 185 445
Cuts at: 4456 325
Size: 1498
Fragments arranged by size:
1498 1255 1097 774 445 205 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 2243 2395 1316
Size: 927 152 4550
Fragments arranged by size:
4550 927 152
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2009 2125 2624 2954 1870
Size: 139 116 499 330 4545
Fragments arranged by size:
4545 499 330 139 116
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI SacI SacII SapI SexAI
SfiI SwaI XcmI