Restriction map for
pFN21AE3118
(Circular) (Six-base) MAPSORT of: pFN21AE3118.seq Check: 8486 from: 1 to: 5581
pFN21AE3118 5581 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2773 3644 5028 5296 1632
Size: 1141 871 1384 268 1917
Fragments arranged by size:
1917 1384 1141 871 268
AflII C'TTAA_G
Cuts at: 820 1017 2452 820
Size: 197 1435 3949
Fragments arranged by size:
3949 1435 197
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 3324 4896 3324
Size: 1572 4009
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2101 2312 2890 3116 3268 5377 1493
Size: 608 211 578 226 152 2109 1697
Fragments arranged by size:
2109 1697 608 578 226 211 152
AseI AT'TA_AT
Cuts at: 160 1032 2741 4135 160
Size: 872 1709 1394 1606
Fragments arranged by size:
1709 1606 1394 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2906 1870
Size: 73 249 714 4545
Fragments arranged by size:
4545 714 249 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2077 2084 2902 4357
Size: 325 217 627 224 66 7 818 1455
Cuts at: 4357 618
Size: 1842
Fragments arranged by size:
1842 1455 818 627 325 224 217 66
7
BanII G_rGCy'C
Cuts at: 1391 2217 2447 2595 2900 1391
Size: 826 230 148 305 4072
Fragments arranged by size:
4072 826 305 230 148
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 5581
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 1903
Size: 34 1835 34 3678
Fragments arranged by size:
3678 1835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2712 5576 2712
Size: 2864 2717
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 2016 3642 4798 1790
Size: 226 1626 1156 2573
Fragments arranged by size:
2573 1626 1156 226
BmgBI CAC'GTC
Cuts at: 1318 2221 1318
Size: 903 4678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2268 2268
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4241 1239
Size: 3002 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2290 2461 3706 4571 4869 2290
Size: 171 1245 865 298 3002
Fragments arranged by size:
3002 1245 865 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 2523 4244 882
Size: 617 540 484 1721 2219
Fragments arranged by size:
2219 1721 617 540 484
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2629 3388 3770
Size: 53 83 186 936 552 544 759 382
Cuts at: 3770 4405 275
Size: 635 1451
Fragments arranged by size:
1451 936 759 635 552 544 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2131 4014 4412 4686 4833 5244 2131
Size: 1883 398 274 147 411 2468
Fragments arranged by size:
2468 1883 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 2255 2285 2654
Size: 30 165 30 619 30 203 30 369
Cuts at: 2654 2684 1178
Size: 30 4075
Fragments arranged by size:
4075 619 369 203 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 1186
Size: 1126 4455
BseYI C'CCAG_C
Cuts at: 2641 4784 5280 2641
Size: 2143 496 2942
Fragments arranged by size:
2942 2143 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2729 1163
Size: 573 993 4015
Fragments arranged by size:
4015 993 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 3642 3727 4798 2447
Size: 1195 85 1071 3230
Fragments arranged by size:
3230 1195 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
BsmBI CGTCTCn'nnnn_
Cuts at: 2324 2636 2324
Size: 312 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2217 2447 2595 2900 3642 3727
Size: 399 226 201 230 148 305 742 85
Cuts at: 3727 4798 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 230 226 201
148 85
BspEI T'CCGG_A
Cuts at: 2131 5244 2131
Size: 3113 2468
BspHI T'CATG_A
Cuts at: 2412 3470 5200 2412
Size: 1058 1730 2793
Fragments arranged by size:
2793 1730 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2300 2869 2936 844
Size: 1456 569 67 3489
Fragments arranged by size:
3489 1456 569 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4225 4412 1700
Size: 110 2415 187 2869
Fragments arranged by size:
2869 2415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2729 2023
Size: 706 4875
BstYI r'GATC_y
Cuts at: 1972 2239 2338 2712 2911 3278 3663 3680 5132
Size: 267 99 374 199 367 385 17 1452
Cuts at: 5132 5218 5230 5576 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 374 367 346 267 199
99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2533 513
Size: 552 939 12 517 3561
Fragments arranged by size:
3561 939 552 517 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2792 3511 1353
Size: 699 740 719 3423
Fragments arranged by size:
3423 740 719 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 4168 4542 4688 387
Size: 1064 138 546 2033 374 146 1280
Fragments arranged by size:
2033 1280 1064 546 374 146 138
EcoICRI GAG'CTC
Cuts at: 2445 2445
Size: 5581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2773 3644 4241 5028 5296 1239
Size: 393 1141 871 597 787 268 1524
Fragments arranged by size:
1524 1141 871 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2424 2424
Size: 5581
EcoRI G'AATT_C
Cuts at: 2312 2890 3268 2312
Size: 578 378 4625
Fragments arranged by size:
4625 578 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 5581
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2088 4728 1678
Size: 410 2640 2531
Fragments arranged by size:
2640 2531 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2710 2742 4222 4254 4296
Size: 32 135 32 1138 32 1480 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 1480 1138 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3539 3898 4057 518
Size: 648 843 1530 359 159 2042
Fragments arranged by size:
2042 1530 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2621 2997 3674 4822 5067 1606
Size: 448 567 376 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 448 376 245
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 2004 2533 513
Size: 552 939 529 3561
Fragments arranged by size:
3561 939 552 529
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NruI TCG'CGA
Cuts at: 2068 2068
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2497 2695 2939 4484 1132
Size: 97 1268 198 244 1545 2229
Fragments arranged by size:
2229 1545 1268 244 198 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2424 2424
Size: 5581
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2933 830
Size: 2103 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2621 2621
Size: 5581
SacI G_AGCT'C
Cuts at: 2447 2447
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SapI GCTCTTCn'nnn_
Cuts at: 2792 2792
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2929 4064 4745 4936 826
Size: 219 1884 1135 681 191 1471
Fragments arranged by size:
1884 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 5581
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2305 2440 2452 3685 4586 4848
Size: 197 926 362 135 12 1233 901 262
Cuts at: 4848 820
Size: 1553
Fragments arranged by size:
1553 1233 926 901 362 262 197 135
12
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2533 3020 513
Size: 552 939 529 487 3074
Fragments arranged by size:
3074 939 552 529 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2654 2686 5541 5573 2255
Size: 32 367 32 2855 32 2263
Fragments arranged by size:
2855 2263 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI