Restriction map for
pFN21AE3117
(Circular) (Six-base) MAPSORT of: pFN21AE3117.seq Check: 6561 from: 1 to: 5578
pFN21AE3117 5578 bp
With 182 enzymes: SgfI *
March 28, 2012 12:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 2702
Size: 5578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2160 2899 2160
Size: 739 4839
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2236 3641 5025 5293 1632
Size: 604 1405 1384 268 1917
Fragments arranged by size:
1917 1405 1384 604 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 2221 4307 2221
Size: 2086 3492
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2213 1184
Size: 1029 4549
AlwNI CAG_nnn'CTG
Cuts at: 2045 2328 2371 2519 2686 3321 4893 2045
Size: 283 43 148 167 635 1572 2730
Fragments arranged by size:
2730 1572 635 283 167 148 43
ApaI G_GGCC'C
Cuts at: 2092 2092
Size: 5578
ApaLI G'TGCA_C
Cuts at: 2751 3635 4791 2751
Size: 884 1156 3538
Fragments arranged by size:
3538 1156 884
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2903 1870
Size: 73 511 449 4545
Fragments arranged by size:
4545 511 449 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2160 2714 2899 4354 618
Size: 325 217 627 373 554 185 1455 1842
Fragments arranged by size:
1842 1455 627 554 373 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2092 2461 2568 2897 1391
Size: 701 369 107 329 4072
Fragments arranged by size:
4072 701 369 329 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2426 3470 4680 2426
Size: 1044 1210 3324
Fragments arranged by size:
3324 1210 1044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2541 4189 136
Size: 107 122 71 631 605 869 1648 1525
Fragments arranged by size:
1648 1525 869 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2853 2995 2853
Size: 142 5436
Bme1580I G_kGCm'C
Cuts at: 1790 2092 2755 3639 4795 1790
Size: 302 663 884 1156 2573
Fragments arranged by size:
2573 1156 884 663 302
BmgBI CAC'GTC
Cuts at: 1318 2590 1318
Size: 1272 4306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2605 3309 4267
Size: 416 53 83 399 24 1188 704 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1188 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2338 2708 4238 1239
Size: 824 275 370 1530 2579
Fragments arranged by size:
2579 1530 824 370 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2359 3703 4568 4866 2359
Size: 1344 865 298 3071
Fragments arranged by size:
3071 1344 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2260 3369 493
Size: 711 1056 1109 2702
Fragments arranged by size:
2702 1109 1056 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3385 3767 4402 275
Size: 53 83 186 936 1852 382 635 1451
Fragments arranged by size:
1852 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5353
Fragments arranged by size:
5353 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2402 1186
Size: 1069 147 4362
Fragments arranged by size:
4362 1069 147
BseYI C'CCAG_C
Cuts at: 4781 5277 4781
Size: 496 5082
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5005
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2071 2265 2556 2568 2643 2742 2755 3639 3724
Size: 194 291 12 75 99 13 884 85
Cuts at: 3724 4795 2071
Size: 1071 2854
Fragments arranged by size:
2854 1071 884 291 194 99 85 75
13 12
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2092 2265 2461 2556 2568 2643
Size: 399 281 21 173 196 95 12 75
Cuts at: 2643 2742 2755 2897 3639 3724 4795 1391
Size: 99 13 142 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 281 196 173 142
99 95 85 75 21 13 12
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 2702 2933 844
Size: 1841 17 231 3489
Fragments arranged by size:
3489 1841 231 17
BsrBI CCG'CTC
Cuts at: 1394 1823 2245 2494 3465 1394
Size: 429 422 249 971 3507
Fragments arranged by size:
3507 971 429 422 249
BsrDI GCAATG_nn'
Cuts at: 65 1248 2115 2147 4073 4247 65
Size: 1183 867 32 1926 174 1396
Fragments arranged by size:
1926 1396 1183 867 174 32
BsrFI r'CCGG_y
Cuts at: 1700 1810 2163 2540 2652 4222 4409 1700
Size: 110 353 377 112 1570 187 2869
Fragments arranged by size:
2869 1570 377 353 187 112 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2328 794
Size: 1534 4044
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 2282 1184
Size: 1098 4480
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2725 2813 2122
Size: 603 88 4887
Fragments arranged by size:
4887 603 88
BstYI r'GATC_y
Cuts at: 1972 2367 2908 3275 3660 3677 5129 5215 5227
Size: 395 541 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 541 395 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2669 2669
Size: 5578
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 513
Size: 552 939 11 4076
Fragments arranged by size:
4076 939 552 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 3094 3888 3908 2520
Size: 574 794 20 4190
Fragments arranged by size:
4190 794 574 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2538 2654 2785
Size: 54 1425 50 275 18 708 116 131
Cuts at: 2785 3025 3914 5543 8
Size: 240 889 1629 43
Fragments arranged by size:
1629 1425 889 708 275 240 131 116
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2747 3508 1353
Size: 665 729 761 3423
Fragments arranged by size:
3423 761 729 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2522 4165 4539 4685 387
Size: 1064 138 933 1643 374 146 1280
Fragments arranged by size:
1643 1280 1064 933 374 146 138
EcoICRI GAG'CTC
Cuts at: 2566 2566
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2236 2338 2708 3641 4238 5025
Size: 393 431 173 102 370 933 597 787
Cuts at: 5025 5293 1239
Size: 268 1524
Fragments arranged by size:
1524 933 787 597 431 393 370 268
173 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2358 2358
Size: 5578
EcoO109I rG'GnC_Cy
Cuts at: 2042 2088 2351 2395 2497 2674 2042
Size: 46 263 44 102 177 4946
Fragments arranged by size:
4946 263 177 102 46 44
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 2189 4084 2189
Size: 1895 3683
FspAI rTGC'GCAy
Cuts at: 2189 2189
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 4725 1678
Size: 3047 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 4219 4251 4293
Size: 32 135 32 635 32 1980 32 42
Cuts at: 4293 4325 1373
Size: 32 2626
Fragments arranged by size:
2626 1980 635 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2164 2903 2164
Size: 739 4839
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3536 3895 4054 518
Size: 648 2370 359 159 2042
Fragments arranged by size:
2370 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2328 2620 2683 2796 2994 3671 4819 5064
Size: 722 292 63 113 198 677 1148 245
Cuts at: 5064 1606
Size: 2120
Fragments arranged by size:
2120 1148 722 677 292 245 198 113
63
NaeI GCC'GGC
Cuts at: 2542 2542
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 2004 2015 513
Size: 552 939 11 4076
Fragments arranged by size:
4076 939 552 11
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2540 2540
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2085 2468 1348
Size: 737 383 4458
Fragments arranged by size:
4458 737 383
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2674 2497
Size: 177 5401
PshAI GACnn'nnGTC
Cuts at: 1613 2289 1613
Size: 676 4902
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PspOMI G'GGCC_C
Cuts at: 2088 2088
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2682 2930 830
Size: 1852 248 3478
Fragments arranged by size:
3478 1852 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2620 2683 2620
Size: 63 5515
SacI G_AGCT'C
Cuts at: 2568 2568
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SanDI GG'GwC_CC
Cuts at: 2497 2497
Size: 5578
SapI GCTCTTCn'nnn_
Cuts at: 2747 2747
Size: 5578
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 2336 3826 5538 2336
Size: 1490 1712 2376
Fragments arranged by size:
2376 1712 1490
SexAI A'CCwGG_T
Cuts at: 2634 2634
Size: 5578
SfcI C'TryA_G
Cuts at: 826 1045 2678 2926 4061 4742 4933 826
Size: 219 1633 248 1135 681 191 1471
Fragments arranged by size:
1633 1471 1135 681 248 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 3682 4583 4845 820
Size: 197 926 395 1344 901 262 1553
Fragments arranged by size:
1553 1344 926 901 395 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2004 2015 3017 513
Size: 552 939 11 1002 3074
Fragments arranged by size:
3074 1002 939 552 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2624 3605 3622 3775 3960 4405
Size: 1255 702 342 981 17 153 185 445
Cuts at: 4405 325
Size: 1498
Fragments arranged by size:
1498 1255 981 702 445 342 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 3824 5536
Size: 274 80 33 51 1443 357 1490 1712
Cuts at: 5536 96
Size: 138
Fragments arranged by size:
1712 1490 1443 357 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2803 2835 5538 5570 2803
Size: 32 2703 32 2811
Fragments arranged by size:
2811 2703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 4790
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI