Restriction map for
pFN21AE3115
(Circular) (Six-base) MAPSORT of: pFN21AE3115.seq Check: 1440 from: 1 to: 5578
pFN21AE3115 5578 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2676 3641 5025 5293 1632
Size: 600 444 965 1384 268 1917
Fragments arranged by size:
1917 1384 965 600 444 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2136 1184
Size: 952 4626
AlwNI CAG_nnn'CTG
Cuts at: 3321 4893 3321
Size: 1572 4006
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2300 2609 2887 3113 3265 5374 1493
Size: 807 309 278 226 152 2109 1697
Fragments arranged by size:
2109 1697 807 309 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2069 2700 2903 1870
Size: 73 68 58 631 203 4545
Fragments arranged by size:
4545 631 203 73 68 58
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 4354 618
Size: 325 217 627 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2674 2810 2897 1391
Size: 621 662 136 87 4072
Fragments arranged by size:
4072 662 621 136 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 4091
Fragments arranged by size:
4091 971 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 3639 4795 1790
Size: 1849 1156 2573
Fragments arranged by size:
2573 1849 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5578
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2521 4238 1239
Size: 1282 1717 2579
Fragments arranged by size:
2579 1717 1282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2658 2794 3703 4568 4866 2658
Size: 136 909 865 298 3370
Fragments arranged by size:
3370 909 865 298 136
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3385 3767 4402 275
Size: 53 83 186 936 1852 382 635 1451
Fragments arranged by size:
1852 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2815 2845 1178
Size: 30 165 30 1412 30 3911
Fragments arranged by size:
3911 1412 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5578
BseYI C'CCAG_C
Cuts at: 2098 2842 4781 5277 2098
Size: 744 1939 496 2399
Fragments arranged by size:
2399 1939 744 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5005
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2054 3028 3789 4817 1540
Size: 275 186 53 974 761 1028 2301
Fragments arranged by size:
2301 1028 974 761 275 186 53
BsiHKAI G_wGCw'C
Cuts at: 2012 3639 3724 4795 2012
Size: 1627 85 1071 2795
Fragments arranged by size:
2795 1627 1071 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2756 2756
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2674 2810 2897 3639 3724 4795
Size: 399 222 662 136 87 742 85 1071
Cuts at: 4795 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 662 399 222 136 87
85
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 844
Size: 2089 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4222 4409 1700
Size: 110 2412 187 2869
Fragments arranged by size:
2869 2412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5578
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 2842
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573
Size: 936 367 385 17 1452 86 12 346
Cuts at: 5573 1972
Size: 1977
Fragments arranged by size:
1977 1452 936 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5026
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2649 3094 3888 3908 2649
Size: 445 794 20 4319
Fragments arranged by size:
4319 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2084 4585 809
Size: 507 768 2501 1802
Fragments arranged by size:
2501 1802 768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543
Size: 54 1425 50 275 18 1195 889 1629
Cuts at: 5543 8
Size: 43
Fragments arranged by size:
1629 1425 1195 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2504 2589 3508 1353
Size: 831 320 85 919 3423
Fragments arranged by size:
3423 919 831 320 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2521 2676 3641 4238 5025 5293
Size: 393 600 289 155 965 597 787 268
Cuts at: 5293 1239
Size: 1524
Fragments arranged by size:
1524 965 787 600 597 393 289 268
155
EcoO109I rG'GnC_Cy
Cuts at: 2103 2862 2103
Size: 759 4819
EcoRI G'AATT_C
Cuts at: 2609 2887 3265 2609
Size: 278 378 4922
Fragments arranged by size:
4922 378 278
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2019 2037 2099 4725 1678
Size: 341 18 62 2626 2531
Fragments arranged by size:
2626 2531 341 62 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 2631 2663 4219
Size: 32 135 32 613 32 414 32 1556
Cuts at: 4219 4251 4293 4325 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1556 613 414 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 2502 3177 2502
Size: 675 4903
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2603 4692 4876 1305
Size: 729 569 2089 184 2007
Fragments arranged by size:
2089 2007 729 569 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 2730 3536 3895 4054 518
Size: 648 952 612 806 359 159 2042
Fragments arranged by size:
2042 952 806 648 612 359 159
MspA1I CmG'CkG
Cuts at: 1606 2434 2994 3671 4819 5064 1606
Size: 828 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 560 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 2103
Size: 5578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2656 2930 830
Size: 1826 274 3478
Fragments arranged by size:
3478 1826 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2434 2434
Size: 5578
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SanDI GG'GwC_CC
Cuts at: 2103 2103
Size: 5578
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2652 2715 2926 4061 4742 4933 826
Size: 219 1607 63 211 1135 681 191 1471
Fragments arranged by size:
1607 1471 1135 681 219 211 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2071 2905 1872
Size: 199 834 4545
Fragments arranged by size:
4545 834 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 2809 3682 4583 4845 820
Size: 197 926 730 136 873 901 262 1553
Fragments arranged by size:
1553 926 901 873 730 262 197 136
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 2317 3502 5
Size: 47 2265 1185 2081
Fragments arranged by size:
2265 2081 1185 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2589 3017 513
Size: 552 1524 428 3074
Fragments arranged by size:
3074 1524 552 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2627 3824 5536
Size: 274 80 33 51 1443 650 1197 1712
Cuts at: 5536 96
Size: 138
Fragments arranged by size:
1712 1443 1197 650 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2815 2847 5538 5570 2815
Size: 32 2691 32 2823
Fragments arranged by size:
2823 2691 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2069 2903 1870
Size: 199 834 4545
Fragments arranged by size:
4545 834 199
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI