Restriction map for
pFN21AE3112
(Circular) (Six-base) MAPSORT of: pFN21AE3112.seq Check: 7576 from: 1 to: 5557
pFN21AE3112 5557 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3620 5004 5272 1632
Size: 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2347 3300 4872 2347
Size: 953 1572 3032
Fragments arranged by size:
3032 1572 953
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2263 2866 3092 3244 5353 1493
Size: 770 603 226 152 2109 1697
Fragments arranged by size:
2109 1697 770 603 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 2882 1870
Size: 73 709 230 4545
Fragments arranged by size:
4545 709 230 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 4333 618
Size: 325 217 627 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2713 2876 1391
Size: 1322 163 4072
Fragments arranged by size:
4072 1322 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 928
Size: 971 264 4322
Fragments arranged by size:
4322 971 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2641 3449 4659 2641
Size: 808 1210 3539
Fragments arranged by size:
3539 1210 808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4168 136
Size: 107 122 71 631 605 2496 1525
Fragments arranged by size:
2496 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4774 1790
Size: 1828 1156 2573
Fragments arranged by size:
2573 1828 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 2835 3288
Size: 416 53 83 399 24 1176 242 453
Cuts at: 3288 4246 442
Size: 958 1753
Fragments arranged by size:
1753 1176 958 453 416 399 242 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2698 4217 1239
Size: 1459 1519 2579
Fragments arranged by size:
2579 1519 1459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2032 3348 493
Size: 711 828 1316 2702
Fragments arranged by size:
2702 1316 828 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2700 2730 1178
Size: 30 165 30 1297 30 4005
Fragments arranged by size:
4005 1297 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2801 1186
Size: 941 674 3942
Fragments arranged by size:
3942 941 674
BseYI C'CCAG_C
Cuts at: 4760 5256 4760
Size: 496 5061
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 1163
Size: 573 613 4371
Fragments arranged by size:
4371 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3768 4796 1540
Size: 275 186 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2713 3618 3703 4774 2049
Size: 664 905 85 1071 2832
Fragments arranged by size:
2832 1071 905 664 85
BsiWI C'GTAC_G
Cuts at: 2028 2028
Size: 5557
BsmI GAATG_Cn'
Cuts at: 2476 3068 3161 2476
Size: 592 93 4872
Fragments arranged by size:
4872 592 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2713 2876 3618 3703 4774 1391
Size: 399 259 664 163 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 259 163 85
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2484 2912 844
Size: 1640 428 3489
Fragments arranged by size:
3489 1640 428
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 1394
Size: 429 1621 3507
Fragments arranged by size:
3507 1621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2355 4052 4226 65
Size: 1183 1107 1697 174 1396
Fragments arranged by size:
1697 1396 1183 1107 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4201 4388 1700
Size: 110 2391 187 2869
Fragments arranged by size:
2869 2391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2641
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552
Size: 915 367 385 17 1452 86 12 346
Cuts at: 5552 1972
Size: 1977
Fragments arranged by size:
1977 1452 915 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5005
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2451 2697 487
Size: 683 56 1225 246 3347
Fragments arranged by size:
3347 1225 683 246 56
BtsI GCAGTG_nn'
Cuts at: 2606 3073 3867 3887 2606
Size: 467 794 20 4276
Fragments arranged by size:
4276 794 467 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2773 2861 2773
Size: 88 5469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3004 3893 5522
Size: 54 1425 50 275 18 1174 889 1629
Cuts at: 5522 8
Size: 43
Fragments arranged by size:
1629 1425 1174 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2524 3487 1353
Size: 1171 963 3423
Fragments arranged by size:
3423 1171 963
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4144 4518 4664 387
Size: 1064 138 2555 374 146 1280
Fragments arranged by size:
2555 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2711 2711
Size: 5557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2698 3620 4217 5004 5272 1239
Size: 393 1066 922 597 787 268 1524
Fragments arranged by size:
1524 1066 922 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2272 2272
Size: 5557
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2784 2784
Size: 5557
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 4704 1678
Size: 3026 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2901 3147 669
Size: 988 281 753 210 246 3079
Fragments arranged by size:
3079 988 753 281 246 210
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2131 4671 4855 1305
Size: 826 2540 184 2007
Fragments arranged by size:
2540 2007 826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 3515 3874 4033 518
Size: 648 1473 876 359 159 2042
Fragments arranged by size:
2042 1473 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2333 2973 3650 4798 5043 1606
Size: 727 640 677 1148 245 2120
Fragments arranged by size:
2120 1148 727 677 640 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5005
NdeI CA'TA_TG
Cuts at: 387 2459 387
Size: 2072 3485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2542 2915 4460 1132
Size: 97 1313 373 1545 2229
Fragments arranged by size:
2229 1545 1313 373 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2825 1108
Size: 1717 3840
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 2667 3127 2667
Size: 460 5097
PstI C_TGCA'G
Cuts at: 830 2435 2909 830
Size: 1605 474 3478
Fragments arranged by size:
3478 1605 474
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
PvuII CAG'CTG
Cuts at: 2333 2333
Size: 5557
SacI G_AGCT'C
Cuts at: 2713 2713
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2431 2905 4040 4721 4912 826
Size: 219 1386 474 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 681 474 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2652 3661 4562 4824 820
Size: 197 926 709 1009 901 262 1553
Fragments arranged by size:
1553 1009 926 901 709 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2542 2915 1132
Size: 1410 373 3774
Fragments arranged by size:
3774 1410 373
SspI AAT'ATT
Cuts at: 5 52 2724 3481 5
Size: 47 2672 757 2081
Fragments arranged by size:
2672 2081 757 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2996 513
Size: 552 1931 3074
Fragments arranged by size:
3074 1931 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 3803 5515
Size: 274 80 33 51 1443 555 1271 1712
Cuts at: 5515 96
Size: 138
Fragments arranged by size:
1712 1443 1271 555 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2249 2281 5517 5549 2249
Size: 32 3236 32 2257
Fragments arranged by size:
3236 2257 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 2652 1943
Size: 709 4848
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI