Restriction map for
pFN21AE3107
(Circular) (Six-base) MAPSORT of: pFN21AE3107.seq Check: 785 from: 1 to: 5536
pFN21AE3107 5536 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 3599 4983 5251 1632
Size: 400 1567 1384 268 1917
Fragments arranged by size:
1917 1567 1384 400 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2216 3279 4851 2216
Size: 1063 1572 2901
Fragments arranged by size:
2901 1572 1063
ApaI G_GGCC'C
Cuts at: 2118 2118
Size: 5536
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2861 1870
Size: 73 129 789 4545
Fragments arranged by size:
4545 789 129 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2857 4312 618
Size: 325 217 627 462 608 1455 1842
Fragments arranged by size:
1842 1455 627 608 462 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2118 2379 2855 1391
Size: 678 49 261 476 4072
Fragments arranged by size:
4072 678 476 261 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 928
Size: 971 903 3662
Fragments arranged by size:
3662 971 903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 4147 136
Size: 107 122 71 631 605 450 2025 1525
Fragments arranged by size:
2025 1525 631 605 450 122 107 71
BglII A'GATC_T
Cuts at: 2418 5531 2418
Size: 3113 2423
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2118 3597 4753 1790
Size: 328 1479 1156 2573
Fragments arranged by size:
2573 1479 1156 328
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2074 2393 2564 4196 1239
Size: 835 319 171 1632 2579
Fragments arranged by size:
2579 1632 835 319 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3661 4526 4824 3661
Size: 865 298 4373
Fragments arranged by size:
4373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2203 3327 493
Size: 711 999 1124 2702
Fragments arranged by size:
2702 1124 999 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2645 2648 1186
Size: 891 568 3 4074
Fragments arranged by size:
4074 891 568 3
BseYI C'CCAG_C
Cuts at: 4739 5235 4739
Size: 496 5040
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2248 1163
Size: 573 348 164 4451
Fragments arranged by size:
4451 573 348 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2044 3597 3682 4753 2044
Size: 1553 85 1071 2827
Fragments arranged by size:
2827 1553 1071 85
BsmI GAATG_Cn'
Cuts at: 2521 3047 3140 2521
Size: 526 93 4917
Fragments arranged by size:
4917 526 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2069 2118 2379 2855 3597 3682
Size: 399 254 25 49 261 476 742 85
Cuts at: 3682 4753 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 476 399 261 254 85
49 25
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 2802 3425 5155 2802
Size: 623 1730 3183
Fragments arranged by size:
3183 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 844
Size: 2047 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3423 1394
Size: 429 1600 3507
Fragments arranged by size:
3507 1600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 4031 4205 65
Size: 1183 894 1889 174 1396
Fragments arranged by size:
1889 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4180 4367 1700
Size: 110 2370 187 2869
Fragments arranged by size:
2869 2370 187 110
BsrGI T'GTAC_A
Cuts at: 96 2436 96
Size: 2340 3196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 2246 2279 2319 2246
Size: 33 40 5463
Fragments arranged by size:
5463 40 33
BstYI r'GATC_y
Cuts at: 1972 2418 2866 3233 3618 3635 5087 5173 5185
Size: 446 448 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 448 446 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2055 513
Size: 552 939 6 45 3994
Fragments arranged by size:
3994 939 552 45 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 3052 3846 3866 2265
Size: 787 794 20 3935
Fragments arranged by size:
3935 794 787 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2058 2674
Size: 54 1425 50 275 18 177 51 616
Cuts at: 2674 2983 3872 5501 8
Size: 309 889 1629 43
Fragments arranged by size:
1629 1425 889 616 309 275 177 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 3466 1353
Size: 1247 866 3423
Fragments arranged by size:
3423 1247 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2074 2393 2564 3599 4196 4983
Size: 393 400 42 319 171 1035 597 787
Cuts at: 4983 5251 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 597 400 393 319 268
171 42
EcoO109I rG'GnC_Cy
Cuts at: 2115 2115
Size: 5536
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2708 2734 2708
Size: 26 5510
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373
Size: 32 135 32 2605 32 42 32 2626
Fragments arranged by size:
2626 2605 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2733 4650 4834 1305
Size: 1428 1917 184 2007
Fragments arranged by size:
2007 1917 1428 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 2676 10
Size: 54 1425 343 228 616 2870
Fragments arranged by size:
2870 1425 616 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2165 2741 3494 3853 4012 518
Size: 648 981 18 576 753 359 159 2042
Fragments arranged by size:
2042 981 753 648 576 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2012 2371 2668 2952 3629 4777 5022 1606
Size: 406 359 297 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 406 359 297 284 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2055 513
Size: 552 939 51 3994
Fragments arranged by size:
3994 939 552 51
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2894 4439 1132
Size: 97 1665 1545 2229
Fragments arranged by size:
2229 1665 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PspOMI G'GGCC_C
Cuts at: 2114 2114
Size: 5536
PspXI vC'TCGA_Gb
Cuts at: 2072 2072
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2888 830
Size: 2058 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
PvuII CAG'CTG
Cuts at: 2371 2668 2371
Size: 297 5239
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2208 2217 2884 4019 4700 4891 826
Size: 219 1163 9 667 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 681 667 219 191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 3640 4541 4803 820
Size: 197 926 129 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197 129
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2097 1517
Size: 580 4956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2975 513
Size: 552 939 51 920 3074
Fragments arranged by size:
3074 939 920 552 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 3563 3580 3733 3918 4363 325
Size: 1255 687 1296 17 153 185 445 1498
Fragments arranged by size:
1498 1296 1255 687 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2365 2413 2436
Size: 274 80 33 51 1443 388 48 23
Cuts at: 2436 3782 5494 96
Size: 1346 1712 138
Fragments arranged by size:
1712 1443 1346 388 274 138 80 51
48 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2659 2872 2659
Size: 213 5323
XhoI C'TCGA_G
Cuts at: 1943 2072 1943
Size: 129 5407
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI