Restriction map for
pFN21AE3105
(Circular) (Six-base) MAPSORT of: pFN21AE3105.seq Check: 263 from: 1 to: 5482
pFN21AE3105 5482 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2408 3292 278
Size: 53 83 186 936 872 884 2468
Fragments arranged by size:
2468 936 884 872 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2508 3545 4929 5197 1632
Size: 443 433 1037 1384 268 1917
Fragments arranged by size:
1917 1384 1037 443 433 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 2313 4381 5358 1203
Size: 1110 2068 977 1327
Fragments arranged by size:
2068 1327 1110 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 2053 3225 4797 2053
Size: 1172 1572 2738
Fragments arranged by size:
2738 1572 1172
ApaI G_GGCC'C
Cuts at: 2384 2384
Size: 5482
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2213 2791 3017 3169 5278 1493
Size: 720 578 226 152 2109 1697
Fragments arranged by size:
2109 1697 720 578 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2807 1870
Size: 73 186 678 4545
Fragments arranged by size:
4545 678 186 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2676 2696 2803 4258
Size: 325 217 627 302 587 20 107 1455
Cuts at: 4258 618
Size: 1842
Fragments arranged by size:
1842 1455 627 587 325 302 217 107
20
BanII G_rGCy'C
Cuts at: 1391 2032 2112 2358 2384 2661 2801 1391
Size: 641 80 246 26 277 140 4072
Fragments arranged by size:
4072 641 277 246 140 80 26
BbeI G_GCGC'C
Cuts at: 2700 2700
Size: 5482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4511
BbvCI CC'TCA_GC
Cuts at: 2762 2762
Size: 5482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 2244 1548
Size: 696 4786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2384 3543 4699 1790
Size: 594 1159 1156 2573
Fragments arranged by size:
2573 1159 1156 594
BmgBI CAC'GTC
Cuts at: 1318 2569 1318
Size: 1251 4231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2226 2361 3213
Size: 416 53 83 399 24 809 135 852
Cuts at: 3213 4171 442
Size: 958 1753
Fragments arranged by size:
1753 958 852 809 416 399 135 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2645 4142 1239
Size: 1406 1497 2579
Fragments arranged by size:
2579 1497 1406
Bpu10I CC'TnA_GC
Cuts at: 2360 2762 2360
Size: 402 5080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3607 4472 4770 3607
Size: 865 298 4319
Fragments arranged by size:
4319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2316 2758 3273 493
Size: 711 1112 442 515 2702
Fragments arranged by size:
2702 1112 711 515 442
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2405 2697 3289 3671
Size: 53 83 186 936 872 292 592 382
Cuts at: 3671 4306 275
Size: 635 1451
Fragments arranged by size:
1451 936 872 635 592 382 292 186
83 53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2779 1186
Size: 1327 266 3889
Fragments arranged by size:
3889 1327 266
BseYI C'CCAG_C
Cuts at: 4685 5181 4685
Size: 496 4986
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 1163
Size: 573 720 4189
Fragments arranged by size:
4189 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3543 3628 4699 3543
Size: 85 1071 4326
Fragments arranged by size:
4326 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
BsmBI CGTCTCn'nnnn_
Cuts at: 2396 2579 2396
Size: 183 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2112 2358 2384 2661 2801 3543
Size: 399 242 80 246 26 277 140 742
Cuts at: 3543 3628 4699 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 277 246 242 140
85 80 26
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2673 4126 4313 1700
Size: 110 863 1453 187 2869
Fragments arranged by size:
2869 1453 863 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2738 794
Size: 1944 3538
BstBI TT'CG_AA
Cuts at: 2120 4286 2120
Size: 2166 3316
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2680 513
Size: 552 972 643 3315
Fragments arranged by size:
3315 972 643 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2755 487
Size: 683 56 1529 3214
Fragments arranged by size:
3214 1529 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 2998 3792 3812 2439
Size: 559 794 20 4109
Fragments arranged by size:
4109 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447
Size: 54 1425 50 275 18 1099 889 1629
Cuts at: 5447 8
Size: 43
Fragments arranged by size:
1629 1425 1099 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3412 1353
Size: 2059 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2508 2645 3545 4142 4929 5197
Size: 393 443 433 137 900 597 787 268
Cuts at: 5197 1239
Size: 1524
Fragments arranged by size:
1524 900 787 597 443 433 393 268
137
EcoNI CCTnn'n_nnAGG
Cuts at: 2172 2172
Size: 5482
EcoO109I rG'GnC_Cy
Cuts at: 2380 2381 2380
Size: 1 5481
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2700 4629 1678
Size: 1022 1929 2531
Fragments arranged by size:
2531 1929 1022
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373
Size: 32 135 32 2551 32 42 32 2626
Fragments arranged by size:
2626 2551 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KasI G'GCGC_C
Cuts at: 2696 2696
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2057 2388 4596 4780 1305
Size: 752 331 2208 184 2007
Fragments arranged by size:
2208 2007 752 331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3660
Fragments arranged by size:
3660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2614 2898 3575 4723 4968 1606
Size: 1008 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 1008 677 284 245
NarI GG'CG_CC
Cuts at: 2697 2697
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4930
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2630 2840 4385 1132
Size: 97 1401 210 1545 2229
Fragments arranged by size:
2229 1545 1401 210 97
PasI CC'CwG_GG
Cuts at: 1348 2389 1348
Size: 1041 4441
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PmlI CAC'GTG
Cuts at: 2316 2316
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PspOMI G'GGCC_C
Cuts at: 2380 2380
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2834 830
Size: 2004 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2614 2614
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2830 3965 4646 4837 826
Size: 219 1785 1135 681 191 1471
Fragments arranged by size:
1785 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2698 2698
Size: 5482
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3586 4487 4749 820
Size: 197 926 186 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2630 2840 1132
Size: 1498 210 3774
Fragments arranged by size:
3774 1498 210
SspI AAT'ATT
Cuts at: 5 52 2125 3406 5
Size: 47 2073 1281 2081
Fragments arranged by size:
2081 2073 1281 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2921 513
Size: 552 1856 3074
Fragments arranged by size:
3074 1856 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3509 3526 3679 3864 4309 325
Size: 1255 1101 828 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1101 828 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 2129 1943
Size: 186 5296
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 2481 3611 2481
Size: 1130 4352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2406 3290 276
Size: 53 83 186 936 872 884 2468
Fragments arranged by size:
2468 936 884 872 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI