Restriction map for
pFN21AE3104
(Circular) (Six-base) MAPSORT of: pFN21AE3104.seq Check: 8616 from: 1 to: 5530
pFN21AE3104 5530 bp
With 182 enzymes: SgfI *
March 28, 2012 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 3593 4977 5245 1632
Size: 816 1145 1384 268 1917
Fragments arranged by size:
1917 1384 1145 816 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 4429 5406 1203
Size: 3226 977 1327
Fragments arranged by size:
3226 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2400 1184
Size: 1216 4314
AlwNI CAG_nnn'CTG
Cuts at: 3273 4845 3273
Size: 1572 3958
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2060 2839 3065 3217 5326 1493
Size: 567 779 226 152 2109 1697
Fragments arranged by size:
2109 1697 779 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2855 1870
Size: 73 912 4545
Fragments arranged by size:
4545 912 73
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2849 1391
Size: 1458 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 928
Size: 971 210 4349
Fragments arranged by size:
4349 971 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 2255 3591 4747 1790
Size: 465 1336 1156 2573
Fragments arranged by size:
2573 1336 1156 465
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2362 3261 4219
Size: 416 53 83 399 24 945 899 958
Cuts at: 4219 442
Size: 1753
Fragments arranged by size:
1753 958 945 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4190 1239
Size: 2951 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2511 2566 4193 882
Size: 617 1012 55 1627 2219
Fragments arranged by size:
2219 1627 1012 617 55
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 2414 5524 2414
Size: 3110 2420
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3337 3719 4354 275
Size: 53 83 186 936 1804 382 635 1451
Fragments arranged by size:
1804 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3963 4361 4635 4782 5193 3963
Size: 398 274 147 411 4300
Fragments arranged by size:
4300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5305
Fragments arranged by size:
5305 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5530
BseYI C'CCAG_C
Cuts at: 4733 5229 4733
Size: 496 5034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4957
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2980 3741 4769 1540
Size: 275 186 979 761 1028 2301
Fragments arranged by size:
2301 1028 979 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3591 3676 4747 3591
Size: 85 1071 4374
Fragments arranged by size:
4374 1071 85
BsmI GAATG_Cn'
Cuts at: 2389 3041 3134 2389
Size: 652 93 4785
Fragments arranged by size:
4785 652 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2849 3591 3676 4747 1391
Size: 399 465 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 465 399 85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2267 2456 2885 844
Size: 1423 189 429 3489
Fragments arranged by size:
3489 1423 429 189
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2625 4025 4199 65
Size: 1183 1377 1400 174 1396
Fragments arranged by size:
1400 1396 1377 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 3590 4602 3590
Size: 1012 4518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2409 2860 3227 3612 3629 5081 5167 5179
Size: 437 451 367 385 17 1452 86 12
Cuts at: 5179 5525 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 451 437 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4791
Fragments arranged by size:
4791 683 56
BtsI GCAGTG_nn'
Cuts at: 3046 3840 3860 3046
Size: 794 20 4716
Fragments arranged by size:
4716 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2276 2311 529
Size: 35 1712 35 3748
Fragments arranged by size:
3748 1712 35 35
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DraIII CAC_nnn'GTG
Cuts at: 2052 2052
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2137 2977
Size: 54 1425 50 275 18 190 117 840
Cuts at: 2977 3866 5495 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 840 275 190 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2977 1537
Size: 275 1165 4090
Fragments arranged by size:
4090 1165 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3460 1353
Size: 2107 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2448 3593 4190 4977 5245 1239
Size: 393 816 1145 597 787 268 1524
Fragments arranged by size:
1524 1145 816 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2060 2839 3217 2060
Size: 779 378 4373
Fragments arranged by size:
4373 779 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5530
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 4677 1678
Size: 2999 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 2589 2621 4171
Size: 32 135 32 938 32 47 32 1550
Cuts at: 4171 4203 4245 4277 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1550 938 135 47 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 2874 3120 669
Size: 988 281 373 563 246 3079
Fragments arranged by size:
3079 988 563 373 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 2311 3120 2311
Size: 809 4721
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4644 4828 1305
Size: 3339 184 2007
Fragments arranged by size:
3339 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2022 10
Size: 54 1425 343 190 3518
Fragments arranged by size:
3518 1425 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 3488 3847 4006 518
Size: 648 1138 1184 359 159 2042
Fragments arranged by size:
2042 1184 1138 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2946 3623 4771 5016 1606
Size: 1340 677 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4978
NdeI CA'TA_TG
Cuts at: 387 2442 387
Size: 2055 3475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2888 4433 1132
Size: 97 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 1108
Size: 1638 3892
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PstI C_TGCA'G
Cuts at: 830 2882 830
Size: 2052 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 2123 3778 5490 2123
Size: 1655 1712 2163
Fragments arranged by size:
2163 1712 1655
SfcI C'TryA_G
Cuts at: 826 1045 2878 4013 4694 4885 826
Size: 219 1833 1135 681 191 1471
Fragments arranged by size:
1833 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2857 1872
Size: 985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4535 4797 820
Size: 197 926 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2294 2969 513
Size: 552 1229 675 3074
Fragments arranged by size:
3074 1229 675 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3557 3574 3727 3912 4357 325
Size: 1255 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 2322 3776
Size: 274 80 33 51 1443 144 201 1454
Cuts at: 3776 5488 96
Size: 1712 138
Fragments arranged by size:
1712 1454 1443 274 201 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5490
Size: 32 5498
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2855 1870
Size: 985 4545
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI