Restriction map for
pFN21AE3096
(Circular) (Six-base) MAPSORT of: pFN21AE3096.seq Check: 5964 from: 1 to: 5506
pFN21AE3096 5506 bp
With 182 enzymes: SgfI *
March 28, 2012 12:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 5506
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2751 2849 1937
Size: 814 98 4594
Fragments arranged by size:
4594 814 98
Acc65I G'GTAC_C
Cuts at: 2523 2827 2523
Size: 304 5202
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 3569 4953 5221 1632
Size: 623 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 623 268
AfeI AGC'GCT
Cuts at: 2723 2723
Size: 5506
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 2326 2778 3249 4821 2326
Size: 452 471 1572 3011
Fragments arranged by size:
3011 1572 471 452
ApaLI G'TGCA_C
Cuts at: 2143 3563 4719 2143
Size: 1420 1156 2930
Fragments arranged by size:
2930 1420 1156
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2831 1870
Size: 73 525 363 4545
Fragments arranged by size:
4545 525 363 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2213 2523 2827 4282
Size: 325 217 627 406 20 310 304 1455
Cuts at: 4282 618
Size: 1842
Fragments arranged by size:
1842 1455 627 406 325 310 304 217
20
BanII G_rGCy'C
Cuts at: 1391 2027 2156 2825 1391
Size: 636 129 669 4072
Fragments arranged by size:
4072 669 636 129
BbeI G_GCGC'C
Cuts at: 2197 2217 2197
Size: 20 5486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4535
BbvCI CC'TCA_GC
Cuts at: 2041 2041
Size: 5506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3697 3731 1903
Size: 34 1760 34 3678
Fragments arranged by size:
3678 1760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 4117 136
Size: 107 122 71 631 605 508 1937 1525
Fragments arranged by size:
1937 1525 631 605 508 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 2147 3567 4723 1790
Size: 357 1420 1156 2573
Fragments arranged by size:
2573 1420 1156 357
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3237 4195 442
Size: 416 53 83 399 24 1820 958 1753
Fragments arranged by size:
1820 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2708
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 4166 1239
Size: 1439 1488 2579
Fragments arranged by size:
2579 1488 1439
Bpu10I CC'TnA_GC
Cuts at: 2041 2041
Size: 5506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2142 3297 493
Size: 711 938 1155 2702
Fragments arranged by size:
2702 1155 938 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2194 2214 3313 3695
Size: 53 83 186 936 661 20 1099 382
Cuts at: 3695 4330 275
Size: 635 1451
Fragments arranged by size:
1451 1099 936 661 635 382 186 83
53 20
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 1178
Size: 30 165 30 945 30 4306
Fragments arranged by size:
4306 945 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 1186
Size: 1128 4378
BseYI C'CCAG_C
Cuts at: 2157 2316 2493 4709 5205 2157
Size: 159 177 2216 496 2458
Fragments arranged by size:
2458 2216 496 177 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2640 2701 1163
Size: 573 904 61 3968
Fragments arranged by size:
3968 904 573 61
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2260 2956 3717 4745 1540
Size: 275 186 259 696 761 1028 2301
Fragments arranged by size:
2301 1028 761 696 275 259 186
BsiHKAI G_wGCw'C
Cuts at: 2147 2156 3567 3652 4723 2147
Size: 9 1411 85 1071 2930
Fragments arranged by size:
2930 1411 1071 85 9
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2147 2156 2825 3567 3652 4723
Size: 399 237 120 9 669 742 85 1071
Cuts at: 4723 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 669 399 237 120 85
9
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 2861 844
Size: 1467 550 3489
Fragments arranged by size:
3489 1467 550
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 1394
Size: 429 1570 3507
Fragments arranged by size:
3507 1570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2546 4001 4175 65
Size: 1183 1298 1455 174 1396
Fragments arranged by size:
1455 1396 1298 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 2702 3566 4578 2702
Size: 864 1012 3630
Fragments arranged by size:
3630 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 794
Size: 1532 3974
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 2446 1184
Size: 1262 4244
BstYI r'GATC_y
Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501
Size: 864 367 385 17 1452 86 12 346
Cuts at: 5501 1972
Size: 1977
Fragments arranged by size:
1977 1452 864 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2012 2297 2012
Size: 285 5221
BtgI C'CryG_G
Cuts at: 513 1065 2162 513
Size: 552 1097 3857
Fragments arranged by size:
3857 1097 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2500 487
Size: 683 56 1274 3493
Fragments arranged by size:
3493 1274 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2251 3022 3816 3836 2241
Size: 10 771 794 20 3911
Fragments arranged by size:
3911 794 771 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2257 2953 3842
Size: 54 1425 50 275 18 427 696 889
Cuts at: 3842 5471 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 696 427 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2257 2953 1537
Size: 275 445 696 4090
Fragments arranged by size:
4090 696 445 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3436 1353
Size: 2083 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2154 2154
Size: 5506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2678 3569 4166 4953 5221 1239
Size: 393 623 423 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 623 597 423 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2031 2186 2472 2669 2698 2031
Size: 155 286 197 29 4839
Fragments arranged by size:
4839 286 197 155 29
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 2197 2217 2725 4653 1678
Size: 519 20 508 1928 2531
Fragments arranged by size:
2531 1928 519 508 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373
Size: 32 135 32 2575 32 42 32 2626
Fragments arranged by size:
2626 2575 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2193 2213 2193
Size: 20 5486
KpnI G_GTAC'C
Cuts at: 2527 2831 2527
Size: 304 5202
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 4804 1305
Size: 3315 184 2007
Fragments arranged by size:
3315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3684
Fragments arranged by size:
3684 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3464 3823 3982 518
Size: 648 2298 359 159 2042
Fragments arranged by size:
2298 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2922 3599 4747 4992 1606
Size: 1316 677 1148 245 2120
Fragments arranged by size:
2120 1316 1148 677 245
NarI GG'CG_CC
Cuts at: 2194 2214 2194
Size: 20 5486
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4954
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2257 2953 2257
Size: 696 4810
NspI r_CATG'y
Cuts at: 1132 1229 2864 4409 1132
Size: 97 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2281 2632 1348
Size: 933 351 4222
Fragments arranged by size:
4222 933 351
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PfoI T'CCnGG_A
Cuts at: 2366 2366
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2031 2186 2472 2669 2031
Size: 155 286 197 4868
Fragments arranged by size:
4868 286 197 155
PshAI GACnn'nnGTC
Cuts at: 1613 2649 1613
Size: 1036 4470
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2400 2858 830
Size: 1570 458 3478
Fragments arranged by size:
3478 1570 458
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SacI G_AGCT'C
Cuts at: 2156 2156
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SanDI GG'GwC_CC
Cuts at: 2186 2669 2186
Size: 483 5023
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2088 2396 2402 2854 3989 4670 4861
Size: 219 1043 308 6 452 1135 681 191
Cuts at: 4861 826
Size: 1471
Fragments arranged by size:
1471 1135 1043 681 452 308 219 191
6
SfoI GGC'GCC
Cuts at: 2195 2215 2195
Size: 20 5486
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2470 2833 1872
Size: 598 363 4545
Fragments arranged by size:
4545 598 363
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 2456 2585 1517
Size: 939 129 4438
Fragments arranged by size:
4438 939 129
StyI C'CwwG_G
Cuts at: 513 1065 2945 513
Size: 552 1880 3074
Fragments arranged by size:
3074 1880 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 2465 3533 3550 3703 3888 4333
Size: 1255 750 135 1068 17 153 185 445
Cuts at: 4333 325
Size: 1498
Fragments arranged by size:
1498 1255 1068 750 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 3752 5464
Size: 274 80 33 51 1443 792 983 1712
Cuts at: 5464 96
Size: 138
Fragments arranged by size:
1712 1443 983 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 2558 2590 2661 2693 5466 5498 2064
Size: 32 462 32 71 32 2773 32 2072
Fragments arranged by size:
2773 2072 462 71 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2468 2831 1870
Size: 598 363 4545
Fragments arranged by size:
4545 598 363
XmnI GAAnn'nnTTC
Cuts at: 3635 3635
Size: 5506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI