Restriction map for
pFN21AE3095
(Circular) (Six-base) MAPSORT of: pFN21AE3095.seq Check: 3296 from: 1 to: 5494
pFN21AE3095 5494 bp
With 182 enzymes: SgfI *
March 28, 2012 13:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3557 4941 5209 1632
Size: 1925 1384 268 1917
Fragments arranged by size:
1925 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 2484 4393 5370 1203
Size: 1281 1909 977 1327
Fragments arranged by size:
1909 1327 1281 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4024
AlwNI CAG_nnn'CTG
Cuts at: 3237 4809 3237
Size: 1572 3922
ApaI G_GGCC'C
Cuts at: 2573 2573
Size: 5494
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2385 2717 2803 3029 3181 5290 1493
Size: 892 332 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 892 332 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 1870
Size: 73 876 4545
Fragments arranged by size:
4545 876 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2815 4270 618
Size: 325 217 627 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2573 2623 2813 1391
Size: 1182 50 190 4072
Fragments arranged by size:
4072 1182 190 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 928
Size: 971 381 4142
Fragments arranged by size:
4142 971 381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3685 3719 1903
Size: 34 50 34 1664 34 3678
Fragments arranged by size:
3678 1664 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2573 3555 4711 1790
Size: 783 982 1156 2573
Fragments arranged by size:
2573 1156 982 783
BmgBI CAC'GTC
Cuts at: 1318 2542 1318
Size: 1224 4270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2703 3225 4183
Size: 416 53 83 399 24 1286 522 958
Cuts at: 4183 442
Size: 1753
Fragments arranged by size:
1753 1286 958 522 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 4154 1239
Size: 1233 1682 2579
Fragments arranged by size:
2579 1682 1233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3285 493
Size: 711 2081 2702
Fragments arranged by size:
2702 2081 711
BsaBI GATnn'nnATC
Cuts at: 2040 2096 5488 2040
Size: 56 3392 2046
Fragments arranged by size:
3392 2046 56
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3301 3683 4318 275
Size: 53 83 186 936 1768 382 635 1451
Fragments arranged by size:
1768 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5269
Fragments arranged by size:
5269 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 1186
Size: 1285 4209
BseYI C'CCAG_C
Cuts at: 2375 4697 5193 2375
Size: 2322 496 2676
Fragments arranged by size:
2676 2322 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2509 1163
Size: 573 369 404 4148
Fragments arranged by size:
4148 573 404 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2623 3555 3640 4711 2623
Size: 932 85 1071 3406
Fragments arranged by size:
3406 1071 932 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 2623 2813 3555 3640 4711 1391
Size: 399 783 50 190 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 190 85 50
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 2358 3383 5113 2358
Size: 1025 1730 2739
Fragments arranged by size:
2739 1730 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2849 844
Size: 2005 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2696 3989 4163 65
Size: 1183 1448 1293 174 1396
Fragments arranged by size:
1448 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4138 4325 1700
Size: 110 2328 187 2869
Fragments arranged by size:
2869 2328 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489
Size: 852 367 385 17 1452 86 12 346
Cuts at: 5489 1972
Size: 1977
Fragments arranged by size:
1977 1452 852 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2745 2745
Size: 5494
BtgI C'CryG_G
Cuts at: 513 1065 2248 2335 2656 513
Size: 552 1183 87 321 3351
Fragments arranged by size:
3351 1183 552 321 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2497 3010 3804 3824 2334
Size: 163 513 794 20 4004
Fragments arranged by size:
4004 794 513 163 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 2589 2941
Size: 54 1425 50 275 18 477 282 352
Cuts at: 2941 3830 5459 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 477 352 282 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2394 2708 3424 1353
Size: 734 307 314 716 3423
Fragments arranged by size:
3423 734 716 314 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2621 2621
Size: 5494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 3557 4154 4941 5209 1239
Size: 393 840 1085 597 787 268 1524
Fragments arranged by size:
1524 1085 840 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2295 2570 2295
Size: 275 5219
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2447 2447
Size: 5494
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 4641 1678
Size: 2963 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373
Size: 32 135 32 2563 32 42 32 2626
Fragments arranged by size:
2626 2563 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 2591 10
Size: 54 1425 343 477 282 2913
Fragments arranged by size:
2913 1425 477 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 3452 3811 3970 518
Size: 648 1470 816 359 159 2042
Fragments arranged by size:
2042 1470 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2910 3587 4735 4980 1606
Size: 1304 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2248 2335 2656 513
Size: 552 1183 87 321 3351
Fragments arranged by size:
3351 1183 552 321 87
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2488 2852 4397 1132
Size: 97 1259 364 1545 2229
Fragments arranged by size:
2229 1545 1259 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 2484 4393 2484
Size: 1909 3585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2295 2295
Size: 5494
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PspOMI G'GGCC_C
Cuts at: 2569 2569
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
SacI G_AGCT'C
Cuts at: 2623 2623
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SapI GCTCTTCn'nnn_
Cuts at: 2708 2708
Size: 5494
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2842 3977 4658 4849 826
Size: 219 1797 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2248 2304 2335 2656 2768 2933 513
Size: 552 1183 56 31 321 112 165 3074
Fragments arranged by size:
3074 1183 552 321 165 112 56 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2759 3740
Size: 274 80 33 51 1443 189 593 981
Cuts at: 3740 5452 96
Size: 1712 138
Fragments arranged by size:
1712 1443 981 593 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 2543 1316
Size: 1227 4267
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2447 2447
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI