Restriction map for
pFN21AE3094
(Circular) (Six-base) MAPSORT of: pFN21AE3094.seq Check: 1540 from: 1 to: 5494
pFN21AE3094 5494 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2287 2765 3557 4941 5209 1632
Size: 653 2 478 792 1384 268 1917
Fragments arranged by size:
1917 1384 792 653 478 268 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2513 1184
Size: 1329 4165
AlwNI CAG_nnn'CTG
Cuts at: 2602 3237 4809 2602
Size: 635 1572 3287
Fragments arranged by size:
3287 1572 635
ApaI G_GGCC'C
Cuts at: 2213 2213
Size: 5494
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2803 3029 3181 5290 1493
Size: 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2329 2431 2819 1870
Size: 73 386 102 388 4545
Fragments arranged by size:
4545 388 386 102 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2789 2815 4270 618
Size: 325 217 627 1002 26 1455 1842
Fragments arranged by size:
1842 1455 1002 627 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2092 2176 2213 2688 2813 1391
Size: 701 84 37 475 125 4072
Fragments arranged by size:
4072 701 475 125 84 37
BbeI G_GCGC'C
Cuts at: 2793 2793
Size: 5494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2187 2221 3685 3719 1903
Size: 34 250 34 1464 34 3678
Fragments arranged by size:
3678 1464 250 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2477 4105
Size: 107 122 71 631 605 527 278 1628
Cuts at: 4105 136
Size: 1525
Fragments arranged by size:
1628 1525 631 605 527 278 122 107
71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2228 2911 2228
Size: 683 4811
Bme1580I G_kGCm'C
Cuts at: 1790 2213 3555 4711 1790
Size: 423 1342 1156 2573
Fragments arranged by size:
2573 1342 1156 423
BmgBI CAC'GTC
Cuts at: 1318 2506 1318
Size: 1188 4306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 3225 4183
Size: 416 53 83 399 24 669 1139 958
Cuts at: 4183 442
Size: 1753
Fragments arranged by size:
1753 1139 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2612 4154 1239
Size: 1022 351 1542 2579
Fragments arranged by size:
2579 1542 1022 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2109 2150 2177 4157 882
Size: 617 610 41 27 1980 2219
Fragments arranged by size:
2219 1980 617 610 41 27
BsaAI yAC'GTr
Cuts at: 493 1204 2190 2587 3285 493
Size: 711 986 397 698 2702
Fragments arranged by size:
2702 986 711 698 397
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2790 3301 3683 4318
Size: 53 83 186 936 1257 511 382 635
Cuts at: 4318 275
Size: 1451
Fragments arranged by size:
1451 1257 936 635 511 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2123 3927 4325 4599 4746 5157 2123
Size: 1804 398 274 147 411 2460
Fragments arranged by size:
2460 1804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2163 2174 2193 2204 1178
Size: 30 165 30 760 11 19 11 4468
Fragments arranged by size:
4468 760 165 30 30 19 11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2357 2642 1186
Size: 853 318 285 4038
Fragments arranged by size:
4038 853 318 285
BseYI C'CCAG_C
Cuts at: 2073 2246 4697 5193 2073
Size: 173 2451 496 2374
Fragments arranged by size:
2451 2374 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 2495 2567 2588 1163
Size: 573 750 9 72 21 4069
Fragments arranged by size:
4069 750 573 72 21 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2176 2258 2286 3555 3640 4711 2176
Size: 82 28 1269 85 1071 2959
Fragments arranged by size:
2959 1269 1071 85 82 28
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2176 2213 2258 2286 2688 2813
Size: 399 302 84 37 45 28 402 125
Cuts at: 2813 3555 3640 4711 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 302 125 85
84 45 37 28
BspEI T'CCGG_A
Cuts at: 2123 5157 2123
Size: 3034 2460
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2520 2849 844
Size: 1676 329 3489
Fragments arranged by size:
3489 1676 329
BsrBI CCG'CTC
Cuts at: 1394 1823 2232 3381 1394
Size: 429 409 1149 3507
Fragments arranged by size:
3507 1149 429 409
BsrDI GCAATG_nn'
Cuts at: 65 1248 3989 4163 65
Size: 1183 2741 174 1396
Fragments arranged by size:
2741 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2402 2786 4138 4325 1700
Size: 110 331 261 384 1352 187 2869
Fragments arranged by size:
2869 1352 384 331 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 2320 2449 3554 4566 2320
Size: 129 1105 1012 3248
Fragments arranged by size:
3248 1105 1012 129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 2473 2521 2602 794
Size: 1256 423 48 81 3686
Fragments arranged by size:
3686 1256 423 81 48
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2221 2194
Size: 27 5467
BstYI r'GATC_y
Cuts at: 1972 2133 2824 3191 3576 3593 5045 5131 5143
Size: 161 691 367 385 17 1452 86 12
Cuts at: 5143 5489 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 691 385 367 346 161 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2579 3010 3804 3824 2579
Size: 431 794 20 4249
Fragments arranged by size:
4249 794 431 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3830 5459
Size: 54 1425 50 275 18 1111 889 1629
Cuts at: 5459 8
Size: 43
Fragments arranged by size:
1629 1425 1111 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2261 3424 1353
Size: 893 15 1163 3423
Fragments arranged by size:
3423 1163 893 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2174 2174
Size: 5494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2285 2287 2612 2765 3557 4154
Size: 393 629 24 2 325 153 792 597
Cuts at: 4154 4941 5209 1239
Size: 787 268 1524
Fragments arranged by size:
1524 792 787 629 597 393 325 268
153 24 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2726 2726
Size: 5494
EcoO109I rG'GnC_Cy
Cuts at: 2210 2210
Size: 5494
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 2756 2793 4641 1678
Size: 1078 37 1848 2531
Fragments arranged by size:
2531 1848 1078 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 4135 4167 4209
Size: 32 135 32 577 32 1954 32 42
Cuts at: 4209 4241 1373
Size: 32 2626
Fragments arranged by size:
2626 1954 577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KasI G'GCGC_C
Cuts at: 2789 2789
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3672
Fragments arranged by size:
3672 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2564 3452 3811 3970 518
Size: 648 1329 69 888 359 159 2042
Fragments arranged by size:
2042 1329 888 648 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2289 2599 2910 3587 4735 4980 1606
Size: 683 310 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 311 310 245
NaeI GCC'GGC
Cuts at: 2143 2404 2143
Size: 261 5233
NarI GG'CG_CC
Cuts at: 2790 2790
Size: 5494
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2141 2402 2141
Size: 261 5233
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2206 2423 2852 4397 1132
Size: 97 977 217 429 1545 2229
Fragments arranged by size:
2229 1545 977 429 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PfoI T'CCnGG_A
Cuts at: 2058 2277 2058
Size: 219 5275
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2190 2587 2190
Size: 397 5097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PspOMI G'GGCC_C
Cuts at: 2209 2209
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2517 2846 830
Size: 1687 329 3478
Fragments arranged by size:
3478 1687 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
SacI G_AGCT'C
Cuts at: 2176 2176
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SapI GCTCTTCn'nnn_
Cuts at: 2246 2246
Size: 5494
SbfI CC_TGCA'GG
Cuts at: 2517 2846 2517
Size: 329 5165
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2513 2842 3977 4658 4849 826
Size: 219 1468 329 1135 681 191 1471
Fragments arranged by size:
1471 1468 1135 681 329 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2477 2477
Size: 5494
SfoI GGC'GCC
Cuts at: 2791 2791
Size: 5494
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 2141 1700
Size: 441 5053
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2423 2852 1132
Size: 1291 429 3774
Fragments arranged by size:
3774 1291 429
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 2218 2732 1517
Size: 701 514 4279
Fragments arranged by size:
4279 701 514
StyI C'CwwG_G
Cuts at: 513 1065 2004 2933 513
Size: 552 939 929 3074
Fragments arranged by size:
3074 939 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2174 2195 2206 5454 5486 2163
Size: 11 21 11 3248 32 2171
Fragments arranged by size:
3248 2171 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SrfI SwaI XcmI