Restriction map for
pFN21AE3093
(Circular) (Six-base) MAPSORT of: pFN21AE3093.seq Check: 7982 from: 1 to: 5491
pFN21AE3093 5491 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2733 3301 278
Size: 53 83 186 936 1197 568 2468
Fragments arranged by size:
2468 1197 936 568 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 2834 1937
Size: 802 95 4594
Fragments arranged by size:
4594 802 95
Acc65I G'GTAC_C
Cuts at: 2812 2812
Size: 5491
AclI AA'CG_TT
Cuts at: 1563 3618 3991 1563
Size: 2055 373 3063
Fragments arranged by size:
3063 2055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 3554 4938 5206 1632
Size: 872 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 872 268
AfeI AGC'GCT
Cuts at: 2594 2594
Size: 5491
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5294
AflIII A'CryG_T
Cuts at: 1203 4390 5367 1203
Size: 3187 977 1327
Fragments arranged by size:
3187 1327 977
AgeI A'CCGG_T
Cuts at: 4322 4322
Size: 5491
AhdI GACnn_n'nnGTC
Cuts at: 4220 4220
Size: 5491
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5491
AlwNI CAG_nnn'CTG
Cuts at: 2140 2608 3234 4806 2140
Size: 468 626 1572 2825
Fragments arranged by size:
2825 1572 626 468
ApaLI G'TGCA_C
Cuts at: 3548 4704 3548
Size: 1156 4335
ApoI r'AATT_y
Cuts at: 1493 2800 3026 3178 5287 1493
Size: 1307 226 152 2109 1697
Fragments arranged by size:
2109 1697 1307 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4045 160
Size: 872 3013 1606
Fragments arranged by size:
3013 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2816 1870
Size: 73 207 666 4545
Fragments arranged by size:
4545 666 207 73
BamHI G'GATC_C
Cuts at: 2821 3188 2821
Size: 367 5124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2069 2106 2333 2534
Size: 325 217 627 230 52 37 227 201
Cuts at: 2534 2812 4267 618
Size: 278 1455 1842
Fragments arranged by size:
1842 1455 627 325 278 230 227 217
201 52 37
BanII G_rGCy'C
Cuts at: 1391 2057 2810 1391
Size: 666 753 4072
Fragments arranged by size:
4072 753 666
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 5491
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2118 2152 3682 3716 1903
Size: 34 181 34 1530 34 3678
Fragments arranged by size:
3678 1530 181 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 4593 3383
Size: 1210 4281
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 2294 2318
Size: 107 122 71 631 605 373 249 24
Cuts at: 2318 4102 136
Size: 1784 1525
Fragments arranged by size:
1784 1525 631 605 373 249 122 107
71 24
BglII A'GATC_T
Cuts at: 5486 5486
Size: 5491
BlpI GC'TnA_GC
Cuts at: 2056 2908 2056
Size: 852 4639
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2037 2111 2336 3552 4708 1790
Size: 232 15 74 225 1216 1156 2573
Fragments arranged by size:
2573 1216 1156 232 225 74 15
BmgBI CAC'GTC
Cuts at: 1318 2545 2683 1318
Size: 1227 138 4126
Fragments arranged by size:
4126 1227 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 3222 4180
Size: 416 53 83 399 24 658 1147 958
Cuts at: 4180 442
Size: 1753
Fragments arranged by size:
1753 1147 958 658 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2642 2593
Size: 49 5442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 4151 1239
Size: 1016 1896 2579
Fragments arranged by size:
2579 1896 1016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4481 4779 3616
Size: 865 298 4328
Fragments arranged by size:
4328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4154 882
Size: 617 993 1662 2219
Fragments arranged by size:
2219 1662 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 493
Size: 711 2078 2702
Fragments arranged by size:
2702 2078 711
BsaBI GATnn'nnATC
Cuts at: 5485 5485
Size: 5491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2730 3298 3680
Size: 53 83 186 936 537 660 568 382
Cuts at: 3680 4315 275
Size: 635 1451
Fragments arranged by size:
1451 936 660 635 568 537 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3924 4322 4596 4743 5154 3924
Size: 398 274 147 411 4261
Fragments arranged by size:
4261 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5266
Fragments arranged by size:
5266 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5491
BseYI C'CCAG_C
Cuts at: 2010 2277 2428 4694 5190 2010
Size: 267 151 2266 496 2311
Fragments arranged by size:
2311 2266 496 267 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4918
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2691 2941 3702 4730 1540
Size: 275 186 690 250 761 1028 2301
Fragments arranged by size:
2301 1028 761 690 275 250 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2466 3552 3637 4708 2202
Size: 264 1086 85 1071 2985
Fragments arranged by size:
2985 1086 1071 264 85
BsmI GAATG_Cn'
Cuts at: 3002 3095 3002
Size: 93 5398
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2037 2057 2111 2202 2336 2466
Size: 399 232 15 20 54 91 134 130
Cuts at: 2466 2810 3552 3637 4708 1391
Size: 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 232 134 130
91 85 54 20 15
BspEI T'CCGG_A
Cuts at: 5154 5154
Size: 5491
BspHI T'CATG_A
Cuts at: 3380 5110 3380
Size: 1730 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 2408 2846 844
Size: 1429 135 438 3489
Fragments arranged by size:
3489 1429 438 135
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 3378 1394
Size: 429 364 1191 3507
Fragments arranged by size:
3507 1191 429 364
BsrDI GCAATG_nn'
Cuts at: 65 1248 3986 4160 65
Size: 1183 2738 174 1396
Fragments arranged by size:
2738 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2124 2531 4135 4322 1700
Size: 110 204 110 407 1604 187 2869
Fragments arranged by size:
2869 1604 407 204 187 110 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5491
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5491
BssSI C'ACGA_G
Cuts at: 3551 4563 3551
Size: 1012 4479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2608 794
Size: 1346 468 3677
Fragments arranged by size:
3677 1346 468
BstBI TT'CG_AA
Cuts at: 4295 4295
Size: 5491
BstEII G'GTnAC_C
Cuts at: 1184 2395 1184
Size: 1211 4280
BstYI r'GATC_y
Cuts at: 1972 2821 3188 3573 3590 5042 5128 5140 5486
Size: 849 367 385 17 1452 86 12 346
Cuts at: 5486 1972
Size: 1977
Fragments arranged by size:
1977 1452 849 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2219 2219
Size: 5491
BtgI C'CryG_G
Cuts at: 513 1065 2114 2379 2550 513
Size: 552 1049 265 171 3454
Fragments arranged by size:
3454 1049 552 265 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2778 487
Size: 683 56 1552 3200
Fragments arranged by size:
3200 1552 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 3007 3801 3821 2141
Size: 866 794 20 3811
Fragments arranged by size:
3811 866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5456
DraI TTT'AAA
Cuts at: 2795 2795
Size: 5491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2735 4498 809
Size: 507 1419 1763 1802
Fragments arranged by size:
1802 1763 1419 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2547 2688
Size: 54 1425 50 275 18 456 261 141
Cuts at: 2688 2938 3827 5456 8
Size: 250 889 1629 43
Fragments arranged by size:
1629 1425 889 456 275 261 250 141
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2688 2938 1537
Size: 275 876 250 4090
Fragments arranged by size:
4090 876 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 3421 1353
Size: 743 1325 3423
Fragments arranged by size:
3423 1325 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2341 2474 2569 4078 4452 4598
Size: 1064 138 752 133 95 1509 374 146
Cuts at: 4598 387
Size: 1280
Fragments arranged by size:
1509 1280 1064 752 374 146 138 133
95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2504 3554 4151 4938 5206 1239
Size: 393 623 249 1050 597 787 268 1524
Fragments arranged by size:
1524 1050 787 623 597 393 268 249
EcoNI CCTnn'n_nnAGG
Cuts at: 2673 2673
Size: 5491
EcoRI G'AATT_C
Cuts at: 2800 3178 2800
Size: 378 5113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 5491
FspI TGC'GCA
Cuts at: 3997 3997
Size: 5491
HaeII r_GCGC'y
Cuts at: 1678 2073 2210 2324 2375 2596 4638 1678
Size: 395 137 114 51 221 2042 2531
Fragments arranged by size:
2531 2042 395 221 137 114 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4132 4164 4206 4238 1373
Size: 32 135 32 2560 32 42 32 2626
Fragments arranged by size:
2626 2560 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 3081 669
Size: 988 281 897 246 3079
Fragments arranged by size:
3079 988 897 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5491
HpaI GTT'AAC
Cuts at: 3081 3081
Size: 5491
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 5491
KpnI G_GTAC'C
Cuts at: 2816 2816
Size: 5491
MfeI C'AATT_G
Cuts at: 3090 3090
Size: 5491
MluI A'CGCG_T
Cuts at: 5367 5367
Size: 5491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4605 4789 1305
Size: 3300 184 2007
Fragments arranged by size:
3300 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 10
Size: 54 1425 343 456 3213
Fragments arranged by size:
3213 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3808 3967 518
Size: 648 2283 359 159 2042
Fragments arranged by size:
2283 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2381 2605 2907 3584 4732 4977 1606
Size: 775 224 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 302 245 224
NaeI GCC'GGC
Cuts at: 2126 2533 2126
Size: 407 5084
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 5491
NcoI C'CATG_G
Cuts at: 513 1065 2114 513
Size: 552 1049 3890
Fragments arranged by size:
3890 1049 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5491
NgoMIV G'CCGG_C
Cuts at: 2124 2531 2124
Size: 407 5084
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5491
NotI GC'GGCC_GC
Cuts at: 2938 2938
Size: 5491
NspI r_CATG'y
Cuts at: 1132 1229 2849 4394 1132
Size: 97 1620 1545 2229
Fragments arranged by size:
2229 1620 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2050 2758 1348
Size: 702 708 4081
Fragments arranged by size:
4081 708 702
PciI A'CATG_T
Cuts at: 4390 4390
Size: 5491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 1108
Size: 1176 4315
PmeI GTTT'AAAC
Cuts at: 2795 2795
Size: 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5073 5105 3560
Size: 32 1481 32 3946
Fragments arranged by size:
3946 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5491
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 5491
PstI C_TGCA'G
Cuts at: 830 2604 2843 830
Size: 1774 239 3478
Fragments arranged by size:
3478 1774 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5491
PvuII CAG'CTG
Cuts at: 2605 2605
Size: 5491
SacII CC_GC'GG
Cuts at: 2382 2382
Size: 5491
SalI G'TCGA_C
Cuts at: 1936 2833 1936
Size: 897 4594
SbfI CC_TGCA'GG
Cuts at: 2843 2843
Size: 5491
ScaI AGT'ACT
Cuts at: 3739 5451 3739
Size: 1712 3779
SfcI C'TryA_G
Cuts at: 826 1045 2600 2839 3974 4655 4846 826
Size: 219 1555 239 1135 681 191 1471
Fragments arranged by size:
1555 1471 1135 681 239 219 191
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 5491
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5491
SmaI CCC'GGG
Cuts at: 1872 2818 1872
Size: 946 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3595 4496 4758 820
Size: 197 926 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3282 493
Size: 2789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5491
SphI G_CATG'C
Cuts at: 1132 2849 1132
Size: 1717 3774
SspI AAT'ATT
Cuts at: 5 52 3415 5
Size: 47 3363 2081
Fragments arranged by size:
3363 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5491
StyI C'CwwG_G
Cuts at: 513 1065 2114 2493 2775 2930 513
Size: 552 1049 379 282 155 3074
Fragments arranged by size:
3074 1049 552 379 282 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3518 3535 3688 3873 4318 325
Size: 1255 1938 17 153 185 445 1498
Fragments arranged by size:
1938 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3737 5449 96
Size: 274 80 33 51 1443 1760 1712 138
Fragments arranged by size:
1760 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5451 5483 5451
Size: 32 5459
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5491
XbaI T'CTAG_A
Cuts at: 2827 2827
Size: 5491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 5491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5491
XmaI C'CCGG_G
Cuts at: 1870 2816 1870
Size: 946 4545
XmnI GAAnn'nnTTC
Cuts at: 3620 3620
Size: 5491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2731 3299 276
Size: 53 83 186 936 1197 568 2468
Fragments arranged by size:
2468 1197 936 568 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI