Restriction map for
pFN21AE3092
(Circular) (Six-base) MAPSORT of: pFN21AE3092.seq Check: 8989 from: 1 to: 5491
pFN21AE3092 5491 bp
With 182 enzymes: SgfI *
March 28, 2012 12:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3301 278
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 1937
Size: 897 4594
Acc65I G'GTAC_C
Cuts at: 2542 2812 2542
Size: 270 5221
AclI AA'CG_TT
Cuts at: 1563 3618 3991 1563
Size: 2055 373 3063
Fragments arranged by size:
3063 2055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2681 3554 4938 5206 1632
Size: 485 564 873 1384 268 1917
Fragments arranged by size:
1917 1384 873 564 485 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5294
AflIII A'CryG_T
Cuts at: 1203 4390 5367 1203
Size: 3187 977 1327
Fragments arranged by size:
3187 1327 977
AgeI A'CCGG_T
Cuts at: 4322 4322
Size: 5491
AhdI GACnn_n'nnGTC
Cuts at: 4220 4220
Size: 5491
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5491
AlwNI CAG_nnn'CTG
Cuts at: 3234 4806 3234
Size: 1572 3919
ApaLI G'TGCA_C
Cuts at: 3548 4704 3548
Size: 1156 4335
ApoI r'AATT_y
Cuts at: 1493 2800 3026 3178 5287 1493
Size: 1307 226 152 2109 1697
Fragments arranged by size:
2109 1697 1307 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2226 4045 160
Size: 872 1194 1819 1606
Fragments arranged by size:
1819 1606 1194 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
AvaI C'yCGr_G
Cuts at: 1870 1943 2816 1870
Size: 73 873 4545
Fragments arranged by size:
4545 873 73
BamHI G'GATC_C
Cuts at: 2345 2821 3188 2345
Size: 476 367 4648
Fragments arranged by size:
4648 476 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 2812 4267 618
Size: 325 217 627 755 270 1455 1842
Fragments arranged by size:
1842 1455 755 627 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2810 1391
Size: 1419 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 2777 928
Size: 971 206 672 3642
Fragments arranged by size:
3642 971 672 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3682 3716 1903
Size: 34 1745 34 3678
Fragments arranged by size:
3678 1745 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2668 3383 4593 2668
Size: 715 1210 3566
Fragments arranged by size:
3566 1210 715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4102 136
Size: 107 122 71 631 605 2430 1525
Fragments arranged by size:
2430 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2409 5486 2409
Size: 3077 2414
BlpI GC'TnA_GC
Cuts at: 2908 2908
Size: 5491
Bme1580I G_kGCm'C
Cuts at: 1790 3552 4708 1790
Size: 1762 1156 2573
Fragments arranged by size:
2573 1762 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5491
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3222 4180 442
Size: 416 53 83 399 24 1805 958 1753
Fragments arranged by size:
1805 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4151 1239
Size: 2912 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 3616 4481 4779 2285
Size: 1331 865 298 2997
Fragments arranged by size:
2997 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4154 882
Size: 617 2655 2219
Fragments arranged by size:
2655 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 493
Size: 711 2078 2702
Fragments arranged by size:
2702 2078 711
BsaBI GATnn'nnATC
Cuts at: 5485 5485
Size: 5491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3298 3680 4315 275
Size: 53 83 186 936 1765 382 635 1451
Fragments arranged by size:
1765 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3924 4322 4596 4743 5154 3924
Size: 398 274 147 411 4261
Fragments arranged by size:
4261 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5266
Fragments arranged by size:
5266 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5491
BseYI C'CCAG_C
Cuts at: 4694 5190 4694
Size: 496 4995
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4918
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2941 3702 4730 1540
Size: 275 186 940 761 1028 2301
Fragments arranged by size:
2301 1028 940 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 3552 3637 4708 2571
Size: 981 85 1071 3354
Fragments arranged by size:
3354 1071 981 85
BsmI GAATG_Cn'
Cuts at: 3002 3095 3002
Size: 93 5398
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2571 2810 3552 3637 4708 1391
Size: 399 781 239 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 399 239 85
BspEI T'CCGG_A
Cuts at: 5154 5154
Size: 5491
BspHI T'CATG_A
Cuts at: 3380 5110 3380
Size: 1730 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 844
Size: 2002 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3378 1394
Size: 429 1555 3507
Fragments arranged by size:
3507 1555 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3986 4160 65
Size: 1183 2738 174 1396
Fragments arranged by size:
2738 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4135 4322 1700
Size: 110 2325 187 2869
Fragments arranged by size:
2869 2325 187 110
BsrGI T'GTAC_A
Cuts at: 96 2465 96
Size: 2369 3122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5491
BssSI C'ACGA_G
Cuts at: 3551 4563 3551
Size: 1012 4479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5491
BstBI TT'CG_AA
Cuts at: 4295 4295
Size: 5491
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5491
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5491
BstYI r'GATC_y
Cuts at: 1972 2345 2409 2821 3188 3573 3590 5042 5128
Size: 373 64 412 367 385 17 1452 86
Cuts at: 5128 5140 5486 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 412 385 373 367 346 86
64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4752
Fragments arranged by size:
4752 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 3007 3801 3821 2772
Size: 235 794 20 4442
Fragments arranged by size:
4442 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5456
DraI TTT'AAA
Cuts at: 2394 2795 2394
Size: 401 5090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4498 809
Size: 507 3182 1802
Fragments arranged by size:
3182 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2448 2592
Size: 54 1425 50 275 18 292 326 144
Cuts at: 2592 2727 2938 3827 5456 8
Size: 135 211 889 1629 43
Fragments arranged by size:
1629 1425 889 326 292 275 211 144
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2938 1537
Size: 275 1126 4090
Fragments arranged by size:
4090 1126 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 3421 1353
Size: 903 1165 3423
Fragments arranged by size:
3423 1165 903
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 4078 4452 4598 387
Size: 1064 138 561 1928 374 146 1280
Fragments arranged by size:
1928 1280 1064 561 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2681 3554 4151 4938 5206 1239
Size: 393 485 564 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 564 485 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2334 2334
Size: 5491
EcoRI G'AATT_C
Cuts at: 2800 3178 2800
Size: 378 5113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2801 2801
Size: 5491
FspI TGC'GCA
Cuts at: 3997 3997
Size: 5491
HaeII r_GCGC'y
Cuts at: 1678 4638 1678
Size: 2960 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 2666 2698 4132
Size: 32 135 32 709 32 353 32 1434
Cuts at: 4132 4164 4206 4238 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 709 353 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 3081 669
Size: 988 281 897 246 3079
Fragments arranged by size:
3079 988 897 281 246
HindIII A'AGCT_T
Cuts at: 748 2384 748
Size: 1636 3855
HpaI GTT'AAC
Cuts at: 3081 3081
Size: 5491
KpnI G_GTAC'C
Cuts at: 2546 2816 2546
Size: 270 5221
MfeI C'AATT_G
Cuts at: 3090 3090
Size: 5491
MluI A'CGCG_T
Cuts at: 5367 5367
Size: 5491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4605 4789 1305
Size: 3300 184 2007
Fragments arranged by size:
3300 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 2450 2594 10
Size: 54 1425 343 292 326 144 2907
Fragments arranged by size:
2907 1425 343 326 292 144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3808 3967 518
Size: 648 2283 359 159 2042
Fragments arranged by size:
2283 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2150 2431 2471 2907 3584 4732 4977 1606
Size: 544 281 40 436 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 544 436 281 245 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5491
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5491
NotI GC'GGCC_GC
Cuts at: 2938 2938
Size: 5491
NspI r_CATG'y
Cuts at: 1132 1229 2849 4394 1132
Size: 97 1620 1545 2229
Fragments arranged by size:
2229 1620 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5491
PciI A'CATG_T
Cuts at: 4390 4390
Size: 5491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5491
PmeI GTTT'AAAC
Cuts at: 2795 2795
Size: 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5073 5105 3560
Size: 32 1481 32 3946
Fragments arranged by size:
3946 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2334 2334
Size: 5491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5491
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 5491
PstI C_TGCA'G
Cuts at: 830 2476 2843 830
Size: 1646 367 3478
Fragments arranged by size:
3478 1646 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5491
PvuII CAG'CTG
Cuts at: 2150 2431 2471 2150
Size: 281 40 5170
Fragments arranged by size:
5170 281 40
SalI G'TCGA_C
Cuts at: 1936 2833 1936
Size: 897 4594
SbfI CC_TGCA'GG
Cuts at: 2843 2843
Size: 5491
ScaI AGT'ACT
Cuts at: 3739 5451 3739
Size: 1712 3779
SfcI C'TryA_G
Cuts at: 826 1045 2472 2839 3974 4655 4846 826
Size: 219 1427 367 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5491
SmaI CCC'GGG
Cuts at: 1872 2818 1872
Size: 946 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3595 4496 4758 820
Size: 197 926 357 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3282 493
Size: 2789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5491
SphI G_CATG'C
Cuts at: 1132 2849 1132
Size: 1717 3774
SspI AAT'ATT
Cuts at: 5 52 2224 3415 5
Size: 47 2172 1191 2081
Fragments arranged by size:
2172 2081 1191 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5491
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2235 2670 2785 2930 513
Size: 552 939 6 225 435 115 145 3074
Fragments arranged by size:
3074 939 552 435 225 145 115 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3518 3535 3688 3873 4318 325
Size: 1255 1938 17 153 185 445 1498
Fragments arranged by size:
1938 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2465 3737 5449
Size: 274 80 33 51 1443 488 1272 1712
Cuts at: 5449 96
Size: 138
Fragments arranged by size:
1712 1443 1272 488 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5451 5483 5451
Size: 32 5459
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5491
XbaI T'CTAG_A
Cuts at: 2827 2827
Size: 5491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5491
XmaI C'CCGG_G
Cuts at: 1870 2816 1870
Size: 946 4545
XmnI GAAnn'nnTTC
Cuts at: 3620 3620
Size: 5491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3299 276
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI