Restriction map for
pFN21AE3090
(Circular) (Six-base) MAPSORT of: pFN21AE3090.seq Check: 7968 from: 1 to: 5485
pFN21AE3090 5485 bp
With 182 enzymes: SgfI *
March 28, 2012 12:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3548 4932 5200 1632
Size: 602 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 3228 4800 3228
Size: 1572 3913
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2204 2794 3020 3172 5281 1493
Size: 711 590 226 152 2109 1697
Fragments arranged by size:
2109 1697 711 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 1870
Size: 73 867 4545
Fragments arranged by size:
4545 867 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 2806 4261 618
Size: 325 217 627 908 111 1455 1842
Fragments arranged by size:
1842 1455 908 627 325 217 111
BanII G_rGCy'C
Cuts at: 1391 2326 2804 1391
Size: 935 478 4072
Fragments arranged by size:
4072 935 478
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2730 928
Size: 971 831 3683
Fragments arranged by size:
3683 971 831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BfrBI ATG'CAT
Cuts at: 2442 2442
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2325 2902 2325
Size: 577 4908
Bme1580I G_kGCm'C
Cuts at: 1790 3546 4702 1790
Size: 1756 1156 2573
Fragments arranged by size:
2573 1756 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3216 4174 442
Size: 416 53 83 399 24 1799 958 1753
Fragments arranged by size:
1799 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2532 4145 1239
Size: 1293 1613 2579
Fragments arranged by size:
2579 1613 1293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2181 3610 4475 4773 2181
Size: 1429 865 298 2893
Fragments arranged by size:
2893 1429 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3292 3674 4309 275
Size: 53 83 186 936 1759 382 635 1451
Fragments arranged by size:
1759 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5260
Fragments arranged by size:
5260 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5485
BseYI C'CCAG_C
Cuts at: 4688 5184 4688
Size: 496 4989
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4912
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2326 3546 3631 4702 2326
Size: 1220 85 1071 3109
Fragments arranged by size:
3109 1220 1071 85
BsmI GAATG_Cn'
Cuts at: 2444 2601 2996 3089 2444
Size: 157 395 93 4840
Fragments arranged by size:
4840 395 157 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 2804 3546 3631 4702 1391
Size: 399 536 478 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 478 399 85
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2840 844
Size: 1996 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 3980 4154 65
Size: 1183 940 1792 174 1396
Fragments arranged by size:
1792 1396 1183 940 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480
Size: 843 367 385 17 1452 86 12 346
Cuts at: 5480 1972
Size: 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3994
Fragments arranged by size:
3994 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 487
Size: 683 56 851 3895
Fragments arranged by size:
3895 851 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2731 2789 2731
Size: 58 5427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2503 2932
Size: 54 1425 50 275 18 177 496 429
Cuts at: 2932 3821 5450 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 496 429 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 3415 1353
Size: 1233 829 3423
Fragments arranged by size:
3423 1233 829
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2324 2324
Size: 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2532 3548 4145 4932 5200 1239
Size: 393 602 298 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 787 602 597 393 298 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2058
Size: 5485
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 4632 1678
Size: 2954 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 4126 4158 4200
Size: 32 135 32 880 32 1642 32 42
Cuts at: 4200 4232 1373
Size: 32 2626
Fragments arranged by size:
2626 1642 880 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2109 2829 3075 669
Size: 988 281 171 720 246 3079
Fragments arranged by size:
3079 988 720 281 246 171
HindIII A'AGCT_T
Cuts at: 748 2624 748
Size: 1876 3609
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 2132 3084 2132
Size: 952 4533
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2505 10
Size: 54 1425 343 177 496 2990
Fragments arranged by size:
2990 1425 496 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 3802 3961 518
Size: 648 2277 359 159 2042
Fragments arranged by size:
2277 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2901 3578 4726 4971 1606
Size: 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3994
Fragments arranged by size:
3994 939 552
NdeI CA'TA_TG
Cuts at: 387 2299 387
Size: 1912 3573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NsiI A_TGCA'T
Cuts at: 2444 2444
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2559 2843 4388 1132
Size: 97 1330 284 1545 2229
Fragments arranged by size:
2229 1545 1330 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5485
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2058
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 2634 3055 2634
Size: 421 5064
PstI C_TGCA'G
Cuts at: 830 2101 2837 830
Size: 1271 736 3478
Fragments arranged by size:
3478 1271 736
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SacI G_AGCT'C
Cuts at: 2326 2326
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SanDI GG'GwC_CC
Cuts at: 2058 2058
Size: 5485
SapI GCTCTTCn'nnn_
Cuts at: 2586 2586
Size: 5485
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 2194 2779 3733 5445 2194
Size: 585 954 1712 2234
Fragments arranged by size:
2234 1712 954 585
SfcI C'TryA_G
Cuts at: 826 1045 2097 2833 3968 4649 4840 826
Size: 219 1052 736 1135 681 191 1471
Fragments arranged by size:
1471 1135 1052 736 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 3589 4490 4752 820
Size: 197 926 253 1393 901 262 1553
Fragments arranged by size:
1553 1393 926 901 262 253 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 2615 3409 5
Size: 47 2563 794 2081
Fragments arranged by size:
2563 2081 794 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2004 2924 513
Size: 552 939 920 3074
Fragments arranged by size:
3074 939 920 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2192 2777 3731
Size: 274 80 33 51 1443 215 585 954
Cuts at: 3731 5443 96
Size: 1712 138
Fragments arranged by size:
1712 1443 954 585 274 215 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 2154 3614 2154
Size: 1460 4025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI XcmI