Restriction map for
pFN21AE3086
(Circular) (Six-base) MAPSORT of: pFN21AE3086.seq Check: 2944 from: 1 to: 5458
pFN21AE3086 5458 bp
With 182 enzymes: SgfI *
March 28, 2012 12:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 3521 4905 5173 1632
Size: 713 1176 1384 268 1917
Fragments arranged by size:
1917 1384 1176 713 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 2556 4357 5334 1203
Size: 1353 1801 977 1327
Fragments arranged by size:
1801 1353 1327 977
AgeI A'CCGG_T
Cuts at: 2564 4289 2564
Size: 1725 3733
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 2240 2567 2696 1166
Size: 1074 327 129 3928
Fragments arranged by size:
3928 1074 327 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2258 2585 1184
Size: 1074 327 4057
Fragments arranged by size:
4057 1074 327
AlwNI CAG_nnn'CTG
Cuts at: 2341 3201 4773 2341
Size: 860 1572 3026
Fragments arranged by size:
3026 1572 860
ApaI G_GGCC'C
Cuts at: 2667 2667
Size: 5458
ApaLI G'TGCA_C
Cuts at: 2560 3515 4671 2560
Size: 955 1156 3347
Fragments arranged by size:
3347 1156 955
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2225 2531 2694 2740 2783 1870
Size: 73 110 172 306 163 46 43 4545
Fragments arranged by size:
4545 306 172 163 110 73 46 43
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2249 2441 2609 2779
Size: 325 217 627 378 84 192 168 170
Cuts at: 2779 4234 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 378 325 217 192 170
168 84
BanII G_rGCy'C
Cuts at: 1391 2667 2777 1391
Size: 1276 110 4072
Fragments arranged by size:
4072 1276 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2599 2633 3649 3683 1903
Size: 34 662 34 1016 34 3678
Fragments arranged by size:
3678 1016 662 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2586 4069
Size: 107 122 71 631 605 361 553 1483
Cuts at: 4069 136
Size: 1525
Fragments arranged by size:
1525 1483 631 605 553 361 122 107
71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2564 2612 2667 3519 4675 1790
Size: 380 394 48 55 852 1156 2573
Fragments arranged by size:
2573 1156 852 394 380 55 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2720
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 4118 1239
Size: 811 2068 2579
Fragments arranged by size:
2579 2068 811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2488 3265 3647
Size: 53 83 186 936 515 440 777 382
Cuts at: 3647 4282 275
Size: 635 1451
Fragments arranged by size:
1451 936 777 635 515 440 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2564 3891 4289 4563 4710 5121 2564
Size: 1327 398 274 147 411 2901
Fragments arranged by size:
2901 1327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4342
Fragments arranged by size:
4342 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5458
BseYI C'CCAG_C
Cuts at: 2245 2331 2463 4661 5157 2245
Size: 86 132 2198 496 2546
Fragments arranged by size:
2546 2198 496 132 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2095 2908 3669 4697 1540
Size: 275 186 94 813 761 1028 2301
Fragments arranged by size:
2301 1028 813 761 275 186 94
BsiHKAI G_wGCw'C
Cuts at: 2564 2574 3519 3604 4675 2564
Size: 10 945 85 1071 3347
Fragments arranged by size:
3347 1071 945 85 10
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
BsmBI CGTCTCn'nnnn_
Cuts at: 2495 2495
Size: 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2564 2574 2612 2667 2777 3519
Size: 399 380 394 10 38 55 110 742
Cuts at: 3519 3604 4675 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 380 110 85
55 38 10
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2813 844
Size: 1969 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 3953 4127 65
Size: 1183 1354 1351 174 1396
Fragments arranged by size:
1396 1354 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2438 2459 2564 4102 4289 1700
Size: 110 250 378 21 105 1538 187 2869
Fragments arranged by size:
2869 1538 378 250 187 110 105 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 794
Size: 1654 3804
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2480
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453
Size: 816 367 385 17 1452 86 12 346
Cuts at: 5453 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2261 2261
Size: 5458
BtgI C'CryG_G
Cuts at: 513 1065 2196 2238 2427 2581 2638 513
Size: 552 1131 42 189 154 57 3333
Fragments arranged by size:
3333 1131 552 189 154 57 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 2485 2974 3768 3788 2408
Size: 77 489 794 20 4078
Fragments arranged by size:
4078 794 489 77 20
ClaI AT'CG_AT
Cuts at: 1554 2721 1554
Size: 1167 4291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DraIII CAC_nnn'GTG
Cuts at: 2644 2644
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2199 2457
Size: 54 1425 50 275 18 262 107 258
Cuts at: 2457 2671 2905 3794 5423 8
Size: 214 234 889 1629 43
Fragments arranged by size:
1629 1425 889 275 262 258 234 214
107 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2092 2905 1537
Size: 275 280 813 4090
Fragments arranged by size:
4090 813 280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 3388 1353
Size: 1358 677 3423
Fragments arranged by size:
3423 1358 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2345 3521 4118 4905 5173 1239
Size: 393 418 295 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 787 597 418 393 295 268
EcoO109I rG'GnC_Cy
Cuts at: 2106 2629 2663 2664 2678 2106
Size: 523 34 1 14 4886
Fragments arranged by size:
4886 523 34 14 1
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2705 4605 1678
Size: 1027 1900 2531
Fragments arranged by size:
2531 1900 1027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2703 2735 4099
Size: 32 135 32 692 32 407 32 1364
Cuts at: 4099 4131 4173 4205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1364 692 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2515 2802 3048 669
Size: 988 281 577 287 246 3079
Fragments arranged by size:
3079 988 577 287 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2567 2696 3416 3775 3934 518
Size: 648 1074 327 129 720 359 159 2042
Fragments arranged by size:
2042 1074 720 648 359 327 159 129
MspA1I CmG'CkG
Cuts at: 1606 2198 2205 2365 2583 2874 3551 4699 4944
Size: 592 7 160 218 291 677 1148 245
Cuts at: 4944 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 592 291 245 218 160
7
NaeI GCC'GGC
Cuts at: 2062 2440 2461 2062
Size: 378 21 5059
Fragments arranged by size:
5059 378 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NgoMIV G'CCGG_C
Cuts at: 2060 2438 2459 2060
Size: 378 21 5059
Fragments arranged by size:
5059 378 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2659 1348
Size: 1311 4147
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 3922
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2541 1613
Size: 928 4530
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PspOMI G'GGCC_C
Cuts at: 2663 2663
Size: 5458
PspXI vC'TCGA_Gb
Cuts at: 2053 2053
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2421 2810 830
Size: 1591 389 3478
Fragments arranged by size:
3478 1591 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
PvuII CAG'CTG
Cuts at: 2205 2365 2205
Size: 160 5298
SacII CC_GC'GG
Cuts at: 2199 2584 2199
Size: 385 5073
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SapI GCTCTTCn'nnn_
Cuts at: 2711 2711
Size: 5458
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2417 2806 3941 4622 4813 826
Size: 219 1372 389 1135 681 191 1471
Fragments arranged by size:
1471 1372 1135 681 389 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 2564 1700
Size: 864 4594
SmaI CCC'GGG
Cuts at: 1872 2227 2742 2785 1872
Size: 355 515 43 4545
Fragments arranged by size:
4545 515 355 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3562 4463 4725 820
Size: 197 926 110 1509 901 262 1553
Fragments arranged by size:
1553 1509 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2897 513
Size: 552 1832 3074
Fragments arranged by size:
3074 1832 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 3485 3502 3655 3840 4285 325
Size: 1255 657 1248 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 657 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2710 2742 5418 5450 2710
Size: 32 2676 32 2718
Fragments arranged by size:
2718 2676 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2452 2074
Size: 378 5080
XhoI C'TCGA_G
Cuts at: 1943 2053 1943
Size: 110 5348
XmaI C'CCGG_G
Cuts at: 1870 2225 2740 2783 1870
Size: 355 515 43 4545
Fragments arranged by size:
4545 515 355 43
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SacI SanDI SexAI SfiI
SfoI SrfI SwaI