Restriction map for
pFN21AE3085
(Circular) (Six-base) MAPSORT of: pFN21AE3085.seq Check: 3093 from: 1 to: 5455
pFN21AE3085 5455 bp
With 182 enzymes: SgfI *
March 28, 2012 12:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2636 2636
Size: 5455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 2512 3198 4770 2512
Size: 686 1572 3197
Fragments arranged by size:
3197 1572 686
ApaI G_GGCC'C
Cuts at: 2066 2066
Size: 5455
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2764 2990 3142 5251 1493
Size: 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2553 2694 2780 1870
Size: 73 610 141 86 4545
Fragments arranged by size:
4545 610 141 86 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 4231 618
Size: 325 217 627 989 1455 1842
Fragments arranged by size:
1842 1455 989 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 2774 1391
Size: 675 708 4072
Fragments arranged by size:
4072 708 675
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2644 928
Size: 971 745 3739
Fragments arranged by size:
3739 971 745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3646 3680 1903
Size: 34 50 34 1625 34 3678
Fragments arranged by size:
3678 1625 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3347 4557 2247
Size: 1100 1210 3145
Fragments arranged by size:
3145 1210 1100
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4066 136
Size: 107 122 71 631 605 2394 1525
Fragments arranged by size:
2394 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2066 3516 4672 1790
Size: 276 1450 1156 2573
Fragments arranged by size:
2573 1450 1156 276
BmgBI CAC'GTC
Cuts at: 1318 2524 1318
Size: 1206 4249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3186 4144
Size: 416 53 83 399 24 643 1126 958
Cuts at: 4144 442
Size: 1753
Fragments arranged by size:
1753 1126 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2033 1056
Size: 977 4478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2339
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2505 4115 1239
Size: 792 474 1610 2579
Fragments arranged by size:
2579 1610 792 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3262 3644 4279 275
Size: 53 83 186 936 1729 382 635 1451
Fragments arranged by size:
1729 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 1178
Size: 30 165 30 963 30 4237
Fragments arranged by size:
4237 963 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5455
BseYI C'CCAG_C
Cuts at: 4658 5154 4658
Size: 496 4959
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 4276
Fragments arranged by size:
4276 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 3516 3601 4672 2325
Size: 1191 85 1071 3108
Fragments arranged by size:
3108 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2325 2774 3516 3601 4672 1391
Size: 399 276 259 449 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 276 259 85
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 2810 844
Size: 1792 174 3489
Fragments arranged by size:
3489 1792 174
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3950 4124 65
Size: 1183 2702 174 1396
Fragments arranged by size:
2702 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 2193 4099 4286 1700
Size: 110 267 116 1906 187 2869
Fragments arranged by size:
2869 1906 267 187 116 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 2483 2462
Size: 21 5434
BstYI r'GATC_y
Cuts at: 1972 2300 2785 3152 3537 3554 5006 5092 5104
Size: 328 485 367 385 17 1452 86 12
Cuts at: 5104 5450 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 328 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2177 2177
Size: 5455
BtgI C'CryG_G
Cuts at: 513 1065 2127 2139 2535 513
Size: 552 1062 12 396 3433
Fragments arranged by size:
3433 1062 552 396 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2971 3765 3785 2325
Size: 646 794 20 3995
Fragments arranged by size:
3995 794 646 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420
Size: 54 1425 50 275 18 1072 889 1629
Cuts at: 5420 8
Size: 43
Fragments arranged by size:
1629 1425 1072 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3385 1353
Size: 2032 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4042 4416 4562 387
Size: 1064 138 2453 374 146 1280
Fragments arranged by size:
2453 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2505 3518 4115 4902 5170 1239
Size: 393 399 474 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 787 597 474 399 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2042 2062 2213 2509 2042
Size: 20 151 296 4988
Fragments arranged by size:
4988 296 151 20
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 4602 1678
Size: 2924 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 4096 4128 4170
Size: 32 135 32 879 32 1613 32 42
Cuts at: 4170 4202 1373
Size: 32 2626
Fragments arranged by size:
2626 1613 879 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3413 3772 3931 518
Size: 648 2247 359 159 2042
Fragments arranged by size:
2247 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2634 2871 3548 4696 4941 1606
Size: 1028 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 1028 677 245 237
NaeI GCC'GGC
Cuts at: 2079 2195 2079
Size: 116 5339
NcoI C'CATG_G
Cuts at: 513 1065 2139 2535 513
Size: 552 1074 396 3433
Fragments arranged by size:
3433 1074 552 396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NgoMIV G'CCGG_C
Cuts at: 2077 2193 2077
Size: 116 5339
NheI G'CTAG_C
Cuts at: 1052 2029 1052
Size: 977 4478
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2813 4358 1132
Size: 97 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5455
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5455
PfoI T'CCnGG_A
Cuts at: 2253 2303 2475 2253
Size: 50 172 5233
Fragments arranged by size:
5233 172 50
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 2509
Size: 5455
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PspOMI G'GGCC_C
Cuts at: 2062 2062
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2172 2202 2807 830
Size: 1342 30 605 3478
Fragments arranged by size:
3478 1342 605 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
PvuII CAG'CTG
Cuts at: 2634 2634
Size: 5455
RsrII CG'GwC_CG
Cuts at: 2617 2617
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SexAI A'CCwGG_T
Cuts at: 2361 2361
Size: 5455
SfcI C'TryA_G
Cuts at: 826 1045 2168 2198 2447 2638 2803 3938 4619
Size: 219 1123 30 249 191 165 1135 681
Cuts at: 4619 4810 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1123 681 249 219 191 191
165 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2696 2782 1872
Size: 824 86 4545
Fragments arranged by size:
4545 824 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 3559 4460 4722 820
Size: 197 926 610 1006 901 262 1553
Fragments arranged by size:
1553 1006 926 901 610 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 2445 1517
Size: 928 4527
StyI C'CwwG_G
Cuts at: 513 1065 2139 2385 2455 2535 2563 2665 2894
Size: 552 1074 246 70 80 28 102 229
Cuts at: 2894 513
Size: 3074
Fragments arranged by size:
3074 1074 552 246 229 102 80 70
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 3482 3499 3652 3837 4282 325
Size: 1255 762 1140 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1140 762 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 2542
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 2553 1943
Size: 610 4845
XmaI C'CCGG_G
Cuts at: 1870 2694 2780 1870
Size: 824 86 4545
Fragments arranged by size:
4545 824 86
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI