Restriction map for
pFN21AE3084
(Circular) (Six-base) MAPSORT of: pFN21AE3084.seq Check: 2126 from: 1 to: 5449
pFN21AE3084 5449 bp
With 182 enzymes: SgfI *
March 28, 2012 13:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 2349 4348 5325 1203
Size: 1146 1999 977 1327
Fragments arranged by size:
1999 1327 1146 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 5417
AlwNI CAG_nnn'CTG
Cuts at: 2608 3192 4764 2608
Size: 584 1572 3293
Fragments arranged by size:
3293 1572 584
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 2325 2774 1870
Size: 73 322 60 449 4545
Fragments arranged by size:
4545 449 322 73 60
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2321 2355 2514 2770 4225
Size: 325 217 627 534 34 159 256 1455
Cuts at: 4225 618
Size: 1842
Fragments arranged by size:
1842 1455 627 534 325 256 217 159
34
BanII G_rGCy'C
Cuts at: 1391 2599 2652 2701 2768 1391
Size: 1208 53 49 67 4072
Fragments arranged by size:
4072 1208 67 53 49
BbeI G_GCGC'C
Cuts at: 2325 2325
Size: 5449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2464 928
Size: 971 565 3913
Fragments arranged by size:
3913 971 565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3640 3674 1903
Size: 34 50 34 1619 34 3678
Fragments arranged by size:
3678 1619 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 4684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4060 136
Size: 107 122 71 631 605 2388 1525
Fragments arranged by size:
2388 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2215 3510 4666 1790
Size: 425 1295 1156 2573
Fragments arranged by size:
2573 1295 1156 425
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2413 2445 2741
Size: 416 53 83 399 24 996 32 296
Cuts at: 2741 3180 4138 442
Size: 439 958 1753
Fragments arranged by size:
1753 996 958 439 416 399 296 83
53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2267 2417 2723 4109 1239
Size: 996 32 150 306 1386 2579
Fragments arranged by size:
2579 1386 996 306 150 32
Bpu10I CC'TnA_GC
Cuts at: 2609 2609
Size: 5449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 3574 4439 4737 2101
Size: 1473 865 298 2813
Fragments arranged by size:
2813 1473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 3256 3638 4273
Size: 53 83 186 936 789 934 382 635
Cuts at: 4273 275
Size: 1451
Fragments arranged by size:
1451 936 934 789 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2531 2561 2696
Size: 30 165 30 768 30 330 30 135
Cuts at: 2696 2726 1178
Size: 30 3901
Fragments arranged by size:
3901 768 330 165 135 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 2100 2339 2526 2598 4652 5148 2100
Size: 239 187 72 2054 496 2401
Fragments arranged by size:
2401 2054 496 239 187 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2430 1163
Size: 573 365 329 4182
Fragments arranged by size:
4182 573 365 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 3510 3595 4666 2089
Size: 1421 85 1071 2872
Fragments arranged by size:
2872 1421 1071 85
BsmI GAATG_Cn'
Cuts at: 2024 2960 3053 2024
Size: 936 93 4420
Fragments arranged by size:
4420 936 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2215 2599 2652 2701 2768 3510
Size: 399 299 126 384 53 49 67 742
Cuts at: 3510 3595 4666 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 299 126 85
67 53 49
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2804 844
Size: 1177 783 3489
Fragments arranged by size:
3489 1177 783
BsrBI CCG'CTC
Cuts at: 1394 1823 2591 3336 1394
Size: 429 768 745 3507
Fragments arranged by size:
3507 768 745 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 2346 96
Size: 2250 3199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 2360 3509 4521 2360
Size: 1149 1012 3288
Fragments arranged by size:
3288 1149 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 2051 2051
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444
Size: 807 367 385 17 1452 86 12 346
Cuts at: 5444 1972
Size: 1977
Fragments arranged by size:
1977 1452 807 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2215 513
Size: 552 1150 3747
Fragments arranged by size:
3747 1150 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 2965 3759 3779 2463
Size: 502 794 20 4133
Fragments arranged by size:
4133 794 502 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DraIII CAC_nnn'GTG
Cuts at: 2362 2362
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2896 3785
Size: 54 1425 50 275 18 202 864 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 864 275 202 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3379 1353
Size: 2026 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2413 4036 4410 4556 387
Size: 1064 138 824 1623 374 146 1280
Fragments arranged by size:
1623 1280 1064 824 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2267 2417 2723 3512 4109 4896
Size: 393 603 32 150 306 789 597 787
Cuts at: 4896 5164 1239
Size: 268 1524
Fragments arranged by size:
1524 789 787 603 597 393 306 268
150 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2509 2509
Size: 5449
EcoO109I rG'GnC_Cy
Cuts at: 2047 2055 2605 2047
Size: 8 550 4891
Fragments arranged by size:
4891 550 8
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 5449
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2232 2325 4596 1678
Size: 554 93 2271 2531
Fragments arranged by size:
2531 2271 554 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 4090 4122 4164
Size: 32 135 32 1124 32 1362 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 1362 1124 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 2793 3039 669
Size: 988 281 259 596 246 3079
Fragments arranged by size:
3079 988 596 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KasI G'GCGC_C
Cuts at: 2321 2321
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 4563 4747 1305
Size: 897 2361 184 2007
Fragments arranged by size:
2361 2007 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2034 10
Size: 54 1425 343 202 3425
Fragments arranged by size:
3425 1425 343 202 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3407 3766 3925 518
Size: 648 993 1248 359 159 2042
Fragments arranged by size:
2042 1248 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2456 2520 2624 2665 2865 3542 4690
Size: 702 148 64 104 41 200 677 1148
Cuts at: 4690 4935 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 702 677 245 200 148 104
64 41
NarI GG'CG_CC
Cuts at: 2322 2322
Size: 5449
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 2734 387
Size: 2347 3102
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2353 2807 4352 1132
Size: 97 1124 454 1545 2229
Fragments arranged by size:
2229 1545 1124 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 2349 4348 2349
Size: 1999 3450
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 3518 3550 5031 5063 2531
Size: 32 955 32 1481 32 2917
Fragments arranged by size:
2917 1481 955 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2055
Size: 5449
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2308 2308
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SanDI GG'GwC_CC
Cuts at: 2055 2055
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2797 3932 4613 4804 826
Size: 219 1752 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2323 2323
Size: 5449
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2267 2327 2776 1872
Size: 395 60 449 4545
Fragments arranged by size:
4545 449 395 60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 3553 4454 4716 820
Size: 197 926 137 1473 901 262 1553
Fragments arranged by size:
1553 1473 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2050 2657 2888 513
Size: 552 985 607 231 3074
Fragments arranged by size:
3074 985 607 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3695 5407
Size: 274 80 33 51 1443 369 1349 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1443 1349 369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2265 2325 2774 1870
Size: 395 60 449 4545
Fragments arranged by size:
4545 449 395 60
XmnI GAAnn'nnTTC
Cuts at: 2546 2549 3578 2546
Size: 3 1029 4417
Fragments arranged by size:
4417 1029 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI XcmI