Restriction map for
pFN21AE3081
(Circular) (Six-base) MAPSORT of: pFN21AE3081.seq Check: 6639 from: 1 to: 5434
pFN21AE3081 5434 bp
With 182 enzymes: SgfI *
March 28, 2012 12:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2229 2229
Size: 5434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3497 4881 5149 1632
Size: 695 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1170 695 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 2131 2470 3177 4749 2131
Size: 339 707 1572 2816
Fragments arranged by size:
2816 1572 707 339
ApaI G_GGCC'C
Cuts at: 2579 2579
Size: 5434
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2649 2759 1870
Size: 73 706 110 4545
Fragments arranged by size:
4545 706 110 73
BamHI G'GATC_C
Cuts at: 2764 3131 2764
Size: 367 5067
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 2451 2755 4210 618
Size: 325 217 627 508 156 304 1455 1842
Fragments arranged by size:
1842 1455 627 508 325 304 217 156
BanII G_rGCy'C
Cuts at: 1391 2579 2640 2726 2753 1391
Size: 1188 61 86 27 4072
Fragments arranged by size:
4072 1188 86 61 27
BbeI G_GCGC'C
Cuts at: 2299 2299
Size: 5434
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4045 136
Size: 107 122 71 631 605 2373 1525
Fragments arranged by size:
2373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2039 2117 2851 2039
Size: 78 734 4622
Fragments arranged by size:
4622 734 78
Bme1580I G_kGCm'C
Cuts at: 1790 2557 2579 2691 3495 4651 1790
Size: 767 22 112 804 1156 2573
Fragments arranged by size:
2573 1156 804 767 112 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2567 3165 4123
Size: 416 53 83 399 24 1150 598 958
Cuts at: 4123 442
Size: 1753
Fragments arranged by size:
1753 1150 958 598 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2279
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4094 1239
Size: 2855 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4097 882
Size: 617 2598 2219
Fragments arranged by size:
2598 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2296 3241 3623 4258
Size: 53 83 186 936 763 945 382 635
Cuts at: 4258 275
Size: 1451
Fragments arranged by size:
1451 945 936 763 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3867 4265 4539 4686 5097 3867
Size: 398 274 147 411 4204
Fragments arranged by size:
4204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2513 2516 2519 2528 2531 2534 2537
Size: 1324 3 3 3 9 3 3 3
Cuts at: 2537 2540 2543 2594 1186
Size: 3 3 51 4026
Fragments arranged by size:
4026 1324 51 9 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2112 2362 2371 2598 2684 2727 4637 5133 2112
Size: 250 9 227 86 43 1910 496 2413
Fragments arranged by size:
2413 1910 496 250 227 86 43 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 1163
Size: 573 873 3988
Fragments arranged by size:
3988 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2640 3495 3580 4651 2640
Size: 855 85 1071 3423
Fragments arranged by size:
3423 1071 855 85
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
BsmBI CGTCTCn'nnnn_
Cuts at: 2324 2324
Size: 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2557 2579 2640 2691 2726 2753 3495
Size: 399 767 22 61 51 35 27 742
Cuts at: 3495 3580 4651 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 399 85 61 51
35 27 22
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2789 844
Size: 1385 560 3489
Fragments arranged by size:
3489 1385 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3321 1394
Size: 429 1498 3507
Fragments arranged by size:
3507 1498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4078 4265 1700
Size: 110 2268 187 2869
Fragments arranged by size:
2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 794
Size: 1337 4097
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2428
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429
Size: 792 367 385 17 1452 86 12 346
Cuts at: 5429 1972
Size: 1977
Fragments arranged by size:
1977 1452 792 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3943
Fragments arranged by size:
3943 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2881 3770 5399
Size: 54 1425 50 275 18 1051 889 1629
Cuts at: 5399 8
Size: 43
Fragments arranged by size:
1629 1425 1051 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 3364 1353
Size: 1152 859 3423
Fragments arranged by size:
3423 1152 859
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4395 4541 387
Size: 1064 138 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2638 2638
Size: 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 3497 4094 4881 5149 1239
Size: 393 695 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 695 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2492
Size: 5434
EcoO109I rG'GnC_Cy
Cuts at: 2097 2109 2376 2575 2097
Size: 12 267 199 4956
Fragments arranged by size:
4956 267 199 12
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 2299 2568 4581 1678
Size: 621 269 2013 2531
Fragments arranged by size:
2531 2013 621 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 4075 4107 4149
Size: 32 135 32 900 32 1571 32 42
Cuts at: 4149 4181 1373
Size: 32 2626
Fragments arranged by size:
2626 1571 900 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2778 3024 669
Size: 988 281 840 246 3079
Fragments arranged by size:
3079 988 840 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KasI G'GCGC_C
Cuts at: 2295 2295
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 4732 1305
Size: 3243 184 2007
Fragments arranged by size:
3243 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3612
Fragments arranged by size:
3612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 3751 3910 518
Size: 648 2226 359 159 2042
Fragments arranged by size:
2226 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2388 2413 2449 2850 3527 4675 4920
Size: 510 272 25 36 401 677 1148 245
Cuts at: 4920 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 510 401 272 245 36
25
NarI GG'CG_CC
Cuts at: 2296 2296
Size: 5434
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3943
Fragments arranged by size:
3943 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2102 2251 2380 2549 1348
Size: 754 149 129 169 4233
Fragments arranged by size:
4233 754 169 149 129
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 1108
Size: 1362 4072
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2376 2376
Size: 5434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PspOMI G'GGCC_C
Cuts at: 2575 2575
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2127 2448 2479 2786 830
Size: 1297 321 31 307 3478
Fragments arranged by size:
3478 1297 321 307 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
PvuII CAG'CTG
Cuts at: 2116 2413 2116
Size: 297 5137
SacI G_AGCT'C
Cuts at: 2640 2640
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SanDI GG'GwC_CC
Cuts at: 2376 2376
Size: 5434
SbfI CC_TGCA'GG
Cuts at: 2127 2786 2127
Size: 659 4775
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SfcI C'TryA_G
Cuts at: 826 1045 2123 2444 2475 2782 3917 4598 4789
Size: 219 1078 321 31 307 1135 681 191
Cuts at: 4789 826
Size: 1471
Fragments arranged by size:
1471 1135 1078 681 321 307 219 191
31
SfoI GGC'GCC
Cuts at: 2297 2297
Size: 5434
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4439 4701 820
Size: 197 926 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 1517
Size: 774 4660
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2093 2631 2679 2703 2873
Size: 552 939 22 67 538 48 24 170
Cuts at: 2873 513
Size: 3074
Fragments arranged by size:
3074 939 552 538 170 67 48 24
22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3461 3478 3631 3816 4261 325
Size: 1255 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI