Restriction map for
pFN21AE3079
(Circular) (Six-base) MAPSORT of: pFN21AE3079.seq Check: 8619 from: 1 to: 5422
pFN21AE3079 5422 bp
With 182 enzymes: SgfI *
March 28, 2012 12:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 3485 4869 5137 1632
Size: 584 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 584 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 2696 4321 5298 1203
Size: 1493 1625 977 1327
Fragments arranged by size:
1625 1493 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 2505
Size: 32 5390
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2260 2384 2731 2957 3109 5218 1493
Size: 767 124 347 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 347 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2075 2747 1870
Size: 73 90 42 672 4545
Fragments arranged by size:
4545 672 90 73 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2021 2054 2021
Size: 33 5389
BamHI G'GATC_C
Cuts at: 2619 2752 3119 2619
Size: 133 367 4922
Fragments arranged by size:
4922 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2743 4198 618
Size: 325 217 627 311 645 1455 1842
Fragments arranged by size:
1842 1455 645 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2250 2490 2741 1391
Size: 859 240 251 4072
Fragments arranged by size:
4072 859 251 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4451
BbvCI CC'TCA_GC
Cuts at: 2153 2153
Size: 5422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2049 3314 4524 2049
Size: 1265 1210 2947
Fragments arranged by size:
2947 1265 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BfrBI ATG'CAT
Cuts at: 2639 2639
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 4033 136
Size: 107 122 71 631 605 493 1868 1525
Fragments arranged by size:
1868 1525 631 605 493 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 3483 4639 1790
Size: 1693 1156 2573
Fragments arranged by size:
2573 1693 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 3153 4111
Size: 416 53 83 399 24 734 1002 958
Cuts at: 4111 442
Size: 1753
Fragments arranged by size:
1753 1002 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4082 1239
Size: 2843 2579
Bpu10I CC'TnA_GC
Cuts at: 2153 2153
Size: 5422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 4085 882
Size: 617 762 1824 2219
Fragments arranged by size:
2219 1824 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3229 3611 4246 275
Size: 53 83 186 936 1696 382 635 1451
Fragments arranged by size:
1696 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 1186
Size: 1294 4128
BseYI C'CCAG_C
Cuts at: 2012 2065 2213 4625 5121 2012
Size: 53 148 2412 496 2313
Fragments arranged by size:
2412 2313 496 148 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4849
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 3483 3568 4639 2250
Size: 1233 85 1071 3033
Fragments arranged by size:
3033 1233 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2490 2741 3483 3568 4639 1391
Size: 399 460 240 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 251 240 85
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 844
Size: 1933 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 1394
Size: 429 1486 3507
Fragments arranged by size:
3507 1486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 4066 4253 1700
Size: 110 271 1985 187 2869
Fragments arranged by size:
2869 1985 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 2462 3482 4494 2462
Size: 1020 1012 3390
Fragments arranged by size:
3390 1020 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2619 2752 3119 3504 3521 4973 5059 5071
Size: 647 133 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 647 385 367 346 133 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2160 513
Size: 552 939 156 3775
Fragments arranged by size:
3775 939 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 2634 1554
Size: 1080 4342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 2869 3758
Size: 54 1425 50 275 18 619 420 889
Cuts at: 3758 5387 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 619 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 3352 1353
Size: 995 1004 3423
Fragments arranged by size:
3423 1004 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2549 2617 4009 4383 4529 387
Size: 1064 138 960 68 1392 374 146 1280
Fragments arranged by size:
1392 1280 1064 960 374 146 138 68
EcoICRI GAG'CTC
Cuts at: 2248 2248
Size: 5422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 3485 4082 4869 5137 1239
Size: 393 584 1269 597 787 268 1524
Fragments arranged by size:
1524 1269 787 597 584 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2069 2091 2069
Size: 22 5400
EcoRI G'AATT_C
Cuts at: 2384 2731 3109 2384
Size: 347 378 4697
Fragments arranged by size:
4697 378 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2384 2384
Size: 5422
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 4569 1678
Size: 2891 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 3804
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3739 3898 518
Size: 648 2214 359 159 2042
Fragments arranged by size:
2214 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2838 3515 4663 4908 1606
Size: 418 814 677 1148 245 2120
Fragments arranged by size:
2120 1148 814 677 418 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2160 513
Size: 552 939 156 3775
Fragments arranged by size:
3775 939 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NsiI A_TGCA'T
Cuts at: 2641 2641
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2152 2700 2780 4325 1132
Size: 97 923 548 80 1545 2229
Fragments arranged by size:
2229 1545 923 548 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5422
PciI A'CATG_T
Cuts at: 2696 4321 2696
Size: 1625 3797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2469 3491 3523 5004 5036 2437
Size: 32 1022 32 1481 32 2823
Fragments arranged by size:
2823 1481 1022 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2091
Size: 5422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
SacI G_AGCT'C
Cuts at: 2250 2250
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SanDI GG'GwC_CC
Cuts at: 2091 2091
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 2603 3670 5382 2603
Size: 1067 1712 2643
Fragments arranged by size:
2643 1712 1067
SfcI C'TryA_G
Cuts at: 826 1045 2605 2770 3905 4586 4777 826
Size: 219 1560 165 1135 681 191 1471
Fragments arranged by size:
1560 1471 1135 681 219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2077 2749 1872
Size: 205 672 4545
Fragments arranged by size:
4545 672 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 2549 1517
Size: 1032 4390
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2861 513
Size: 552 939 156 701 3074
Fragments arranged by size:
3074 939 701 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 3449 3466 3619 3804 4249 325
Size: 1255 536 1333 17 153 185 445 1498
Fragments arranged by size:
1498 1333 1255 536 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3668 5380
Size: 274 80 33 51 1443 624 1067 1712
Cuts at: 5380 96
Size: 138
Fragments arranged by size:
1712 1443 1067 624 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2138
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2075 2747 1870
Size: 205 672 4545
Fragments arranged by size:
4545 672 205
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI