Restriction map for
pFN21AE3076
(Circular) (Six-base) MAPSORT of: pFN21AE3076.seq Check: 886 from: 1 to: 5416
pFN21AE3076 5416 bp
With 182 enzymes: SgfI *
March 28, 2012 12:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2361 3226 278
Size: 53 83 186 936 825 865 2468
Fragments arranged by size:
2468 936 865 825 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2180 2737 2180
Size: 557 4859
AclI AA'CG_TT
Cuts at: 1563 2293 3543 3916 1563
Size: 730 1250 373 3063
Fragments arranged by size:
3063 1250 730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2220 3479 4863 5131 1632
Size: 574 14 1259 1384 268 1917
Fragments arranged by size:
1917 1384 1259 574 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 4145 4145
Size: 5416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2671 3159 4731 2671
Size: 488 1572 3356
Fragments arranged by size:
3356 1572 488
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2507 2725 2951 3103 5212 1493
Size: 1014 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 1870
Size: 73 798 4545
Fragments arranged by size:
4545 798 73
BamHI G'GATC_C
Cuts at: 2397 2746 3113 2397
Size: 349 367 4700
Fragments arranged by size:
4700 367 349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2485 2737 4192 618
Size: 325 217 627 393 305 252 1455 1842
Fragments arranged by size:
1842 1455 627 393 325 305 252 217
BanII G_rGCy'C
Cuts at: 1391 2076 2735 1391
Size: 685 659 4072
Fragments arranged by size:
4072 685 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 2338 928
Size: 971 338 101 4006
Fragments arranged by size:
4006 971 338 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4027 136
Size: 107 122 71 631 605 2355 1525
Fragments arranged by size:
2355 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2490 3477 4633 1790
Size: 637 63 987 1156 2573
Fragments arranged by size:
2573 1156 987 637 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2693 4076 1239
Size: 851 603 1383 2579
Fragments arranged by size:
2579 1383 851 603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 3541 4406 4704 2488
Size: 1053 865 298 3200
Fragments arranged by size:
3200 1053 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2098 3207 493
Size: 711 894 1109 2702
Fragments arranged by size:
2702 1109 894 711
BsaBI GATnn'nnATC
Cuts at: 2108 5410 2108
Size: 3302 2114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2358 3223 3605
Size: 53 83 186 936 530 295 865 382
Cuts at: 3605 4240 275
Size: 635 1451
Fragments arranged by size:
1451 936 865 635 530 382 295 186
83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5191
Fragments arranged by size:
5191 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5416
BseYI C'CCAG_C
Cuts at: 4619 5115 4619
Size: 496 4920
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 2684 1163
Size: 573 384 564 3895
Fragments arranged by size:
3895 573 564 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2866 3627 4655 1540
Size: 275 186 51 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2076 3477 3562 4633 2076
Size: 1401 85 1071 2859
Fragments arranged by size:
2859 1401 1071 85
BsmI GAATG_Cn'
Cuts at: 2411 2927 3020 2411
Size: 516 93 4807
Fragments arranged by size:
4807 516 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2427 2490 2735 3477 3562 4633
Size: 399 286 351 63 245 742 85 1071
Cuts at: 4633 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 351 286 245 85
63
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2162 2273 2771 844
Size: 1318 111 498 3489
Fragments arranged by size:
3489 1318 498 111
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2416 3911 4085 65
Size: 1183 1168 1495 174 1396
Fragments arranged by size:
1495 1396 1183 1168 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4060 4247 1700
Size: 110 2250 187 2869
Fragments arranged by size:
2869 2250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2154 2422 794
Size: 1360 268 3788
Fragments arranged by size:
3788 1360 268
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2397 2478 2746 3113 3498 3515 4967 5053
Size: 425 81 268 367 385 17 1452 86
Cuts at: 5053 5065 5411 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 425 385 367 346 268 86
81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2235 2340 513
Size: 552 939 22 209 105 3589
Fragments arranged by size:
3589 939 552 209 105 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2932 3726 3746 2932
Size: 794 20 4602
Fragments arranged by size:
4602 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2547 2863
Size: 54 1425 50 275 18 219 498 316
Cuts at: 2863 3752 5381 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 498 316 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2863 1537
Size: 275 237 814 4090
Fragments arranged by size:
4090 814 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 3346 1353
Size: 1993 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 5416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2206 2220 2693 3479 4076 4863
Size: 393 458 116 14 473 786 597 787
Cuts at: 4863 5131 1239
Size: 268 1524
Fragments arranged by size:
1524 787 786 597 473 458 393 268
116 14
EcoO109I rG'GnC_Cy
Cuts at: 2554 2554
Size: 5416
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5416
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 4563 1678
Size: 2885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373
Size: 32 135 32 2485 32 42 32 2626
Fragments arranged by size:
2626 2485 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2184 2741 2184
Size: 557 4859
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2517 4530 4714 1305
Size: 858 354 2013 184 2007
Fragments arranged by size:
2013 2007 858 354 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2366 3374 3733 3892 518
Size: 648 1047 153 1008 359 159 2042
Fragments arranged by size:
2042 1047 1008 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2054 2832 3509 4657 4902 1606
Size: 448 778 677 1148 245 2120
Fragments arranged by size:
2120 1148 778 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2026 513
Size: 552 939 22 3903
Fragments arranged by size:
3903 939 552 22
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2339 2420 2530 2774 4319 1132
Size: 97 1110 81 110 244 1545 2229
Fragments arranged by size:
2229 1545 1110 244 110 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5416
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 1108
Size: 1467 3949
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2040 2768 830
Size: 1210 728 3478
Fragments arranged by size:
3478 1210 728
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2036 2764 3899 4580 4771 826
Size: 219 991 728 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 728 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 3520 4421 4683 820
Size: 197 926 524 1053 901 262 1553
Fragments arranged by size:
1553 1053 926 901 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 2431 152
Size: 2279 3137
SphI G_CATG'C
Cuts at: 1132 2420 2774 1132
Size: 1288 354 3774
Fragments arranged by size:
3774 1288 354
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2550 2855 513
Size: 552 939 22 524 305 3074
Fragments arranged by size:
3074 939 552 524 305 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3443 3460 3613 3798 4243 325
Size: 1255 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3662 5374 96
Size: 274 80 33 51 1443 1685 1712 138
Fragments arranged by size:
1712 1685 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2408 2446 2212
Size: 196 38 5182
Fragments arranged by size:
5182 196 38
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2359 3224 276
Size: 53 83 186 936 825 865 2468
Fragments arranged by size:
2468 936 865 825 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI