Restriction map for
pFN21AE3073
(Circular) (Six-base) MAPSORT of: pFN21AE3073.seq Check: 9514 from: 1 to: 5407
pFN21AE3073 5407 bp
With 182 enzymes: SgfI *
March 28, 2012 12:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 3470 4854 5122 1632
Size: 749 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1089 749 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 2233 2622 2704 4306 5283 1203
Size: 1030 389 82 1602 977 1327
Fragments arranged by size:
1602 1327 1030 977 389 82
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 1184
Size: 1407 4000
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5375
AlwNI CAG_nnn'CTG
Cuts at: 2407 2563 3150 4722 2407
Size: 156 587 1572 3092
Fragments arranged by size:
3092 1572 587 156
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 1870
Size: 73 789 4545
Fragments arranged by size:
4545 789 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4183 618
Size: 325 217 627 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2514 2726 1391
Size: 1123 212 4072
Fragments arranged by size:
4072 1123 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2363 2397 3598 3632 1903
Size: 34 426 34 1201 34 3678
Fragments arranged by size:
3678 1201 426 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2596 3299 4509 2596
Size: 703 1210 3494
Fragments arranged by size:
3494 1210 703
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5402 2214
Size: 3188 2219
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 2421 1318
Size: 1103 4304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 4067 1239
Size: 968 1860 2579
Fragments arranged by size:
2579 1860 968
Bpu10I CC'TnA_GC
Cuts at: 2564 2564
Size: 5407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2590 2623 3198 493
Size: 711 1386 33 575 2702
Fragments arranged by size:
2702 1386 711 575 33
BsaBI GATnn'nnATC
Cuts at: 2480 5401 2480
Size: 2921 2486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 3596 4231 275
Size: 53 83 186 936 1681 382 635 1451
Fragments arranged by size:
1681 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2088 3840 4238 4512 4659 5070 2088
Size: 1752 398 274 147 411 2425
Fragments arranged by size:
2425 1752 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4285
Fragments arranged by size:
4285 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2486 1186
Size: 1105 195 4107
Fragments arranged by size:
4107 1105 195
BseYI C'CCAG_C
Cuts at: 2197 4610 5106 2197
Size: 2413 496 2498
Fragments arranged by size:
2498 2413 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2479 1163
Size: 573 684 59 4091
Fragments arranged by size:
4091 684 573 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 3468 3553 4624 2283
Size: 1185 85 1071 3066
Fragments arranged by size:
3066 1185 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2514 2726 3468 3553 4624 1391
Size: 399 493 231 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 231 212 85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2664 2762 844
Size: 1820 98 3489
Fragments arranged by size:
3489 1820 98
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2568 3902 4076 65
Size: 1183 1320 1334 174 1396
Fragments arranged by size:
1396 1334 1320 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4051 4238 1700
Size: 110 2241 187 2869
Fragments arranged by size:
2869 2241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2407 794
Size: 1613 3794
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2214 2737 3104 3489 3506 4958 5044 5056
Size: 242 523 367 385 17 1452 86 12
Cuts at: 5056 5402 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 523 385 367 346 242 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2631 2631
Size: 5407
BtgI C'CryG_G
Cuts at: 513 1065 2514 2520 513
Size: 552 1449 6 3400
Fragments arranged by size:
3400 1449 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DraIII CAC_nnn'GTG
Cuts at: 2398 2526 2623 2398
Size: 128 97 5182
Fragments arranged by size:
5182 128 97
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2854 3743
Size: 54 1425 50 275 18 687 337 889
Cuts at: 3743 5372 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 687 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 3337 1353
Size: 845 1139 3423
Fragments arranged by size:
3423 1139 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2381 3470 4067 4854 5122 1239
Size: 393 575 174 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 597 575 393 268 174
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2431 2360
Size: 71 5336
EcoO109I rG'GnC_Cy
Cuts at: 2166 2560 2166
Size: 394 5013
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 4554 1678
Size: 2876 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 4048 4080 4122
Size: 32 135 32 464 32 1980 32 42
Cuts at: 4122 4154 1373
Size: 32 2626
Fragments arranged by size:
2626 1980 464 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 2233 5283 2233
Size: 3050 2357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 10
Size: 54 1425 343 687 2898
Fragments arranged by size:
2898 1425 687 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3724 3883 518
Size: 648 2199 359 159 2042
Fragments arranged by size:
2199 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2823 3500 4648 4893 1606
Size: 1217 677 1148 245 2120
Fragments arranged by size:
2120 1217 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3406
Fragments arranged by size:
3406 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2708 2765 4310 1132
Size: 97 1479 57 1545 2229
Fragments arranged by size:
2229 1545 1479 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 2704 4306 2704
Size: 1602 3805
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PmlI CAC'GTG
Cuts at: 2623 2623
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 3476 3508 4989 5021 2270
Size: 32 1174 32 1481 32 2656
Fragments arranged by size:
2656 1481 1174 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2144 2176 2144
Size: 32 5375
PstI C_TGCA'G
Cuts at: 830 2244 2759 830
Size: 1414 515 3478
Fragments arranged by size:
3478 1414 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 5407
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2161 2240 2755 3890 4571 4762 826
Size: 219 1116 79 515 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 681 515 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2169 2514 2846 513
Size: 552 1104 345 332 3074
Fragments arranged by size:
3074 1104 552 345 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3653 5365 96
Size: 274 80 33 51 1443 1676 1712 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 2156 2366 3536 2156
Size: 210 1170 4027
Fragments arranged by size:
4027 1170 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI