Restriction map for
pFN21AE3072
(Circular) (Six-base) MAPSORT of: pFN21AE3072.seq Check: 4028 from: 1 to: 5395
pFN21AE3072 5395 bp
With 182 enzymes: SgfI *
March 28, 2012 12:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3205 278
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2077 2738 1937
Size: 140 661 4594
Fragments arranged by size:
4594 661 140
Acc65I G'GTAC_C
Cuts at: 2137 2716 2137
Size: 579 4816
AclI AA'CG_TT
Cuts at: 1563 3522 3895 1563
Size: 1959 373 3063
Fragments arranged by size:
3063 1959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 3458 4842 5110 1632
Size: 786 1040 1384 268 1917
Fragments arranged by size:
1917 1384 1040 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5198
AflIII A'CryG_T
Cuts at: 1203 4294 5271 1203
Size: 3091 977 1327
Fragments arranged by size:
3091 1327 977
AgeI A'CCGG_T
Cuts at: 4226 4226
Size: 5395
AhdI GACnn_n'nnGTC
Cuts at: 4124 4124
Size: 5395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5395
AlwNI CAG_nnn'CTG
Cuts at: 3138 4710 3138
Size: 1572 3823
ApaLI G'TGCA_C
Cuts at: 3452 4608 3452
Size: 1156 4239
ApoI r'AATT_y
Cuts at: 1493 2366 2704 2930 3082 5191 1493
Size: 873 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 873 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3949 160
Size: 872 2917 1606
Fragments arranged by size:
2917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2720 1870
Size: 73 99 678 4545
Fragments arranged by size:
4545 678 99 73
BamHI G'GATC_C
Cuts at: 2116 2725 3092 2116
Size: 609 367 4419
Fragments arranged by size:
4419 609 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2479 2716 4171 618
Size: 325 217 627 350 342 237 1455 1842
Fragments arranged by size:
1842 1455 627 350 342 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2396 2714 1391
Size: 1005 318 4072
Fragments arranged by size:
4072 1005 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3586 3620 1903
Size: 34 1649 34 3678
Fragments arranged by size:
3678 1649 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3287 4497 3287
Size: 1210 4185
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4006 136
Size: 107 122 71 631 605 2334 1525
Fragments arranged by size:
2334 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2503 5390 2503
Size: 2887 2508
BlpI GC'TnA_GC
Cuts at: 2812 2812
Size: 5395
Bme1580I G_kGCm'C
Cuts at: 1790 2461 3456 4612 1790
Size: 671 995 1156 2573
Fragments arranged by size:
2573 1156 995 671
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3126 4084 442
Size: 416 53 83 399 24 1709 958 1753
Fragments arranged by size:
1753 1709 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2393 2438 4055 1239
Size: 812 342 45 1617 2579
Fragments arranged by size:
2579 1617 812 342 45
Bpu10I CC'TnA_GC
Cuts at: 2129 2129
Size: 5395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3520 4385 4683 3520
Size: 865 298 4232
Fragments arranged by size:
4232 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 4058 882
Size: 617 945 1614 2219
Fragments arranged by size:
2219 1614 945 617
BsaAI yAC'GTr
Cuts at: 493 1204 3186 493
Size: 711 1982 2702
Fragments arranged by size:
2702 1982 711
BsaBI GATnn'nnATC
Cuts at: 2538 5389 2538
Size: 2851 2544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3202 3584 4219 275
Size: 53 83 186 936 1669 382 635 1451
Fragments arranged by size:
1669 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3828 4226 4500 4647 5058 3828
Size: 398 274 147 411 4165
Fragments arranged by size:
4165 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5170
Fragments arranged by size:
5170 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5395
BseYI C'CCAG_C
Cuts at: 4598 5094 4598
Size: 496 4899
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2175 1163
Size: 573 439 4383
Fragments arranged by size:
4383 573 439
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2845 3606 4634 1540
Size: 275 186 844 761 1028 2301
Fragments arranged by size:
2301 1028 844 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3456 3541 4612 3456
Size: 85 1071 4239
Fragments arranged by size:
4239 1071 85
BsmI GAATG_Cn'
Cuts at: 2906 2999 2906
Size: 93 5302
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2461 2714 3456 3541 4612 1391
Size: 399 606 65 253 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 253 85 65
BspEI T'CCGG_A
Cuts at: 5058 5058
Size: 5395
BspHI T'CATG_A
Cuts at: 2262 2446 3284 5014 2262
Size: 184 838 1730 2643
Fragments arranged by size:
2643 1730 838 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2750 844
Size: 1906 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3282 1394
Size: 429 1459 3507
Fragments arranged by size:
3507 1459 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 3890 4064 65
Size: 1183 1039 1603 174 1396
Fragments arranged by size:
1603 1396 1183 1039 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4039 4226 1700
Size: 110 2229 187 2869
Fragments arranged by size:
2869 2229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5395
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5395
BssSI C'ACGA_G
Cuts at: 3455 4467 3455
Size: 1012 4383
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5395
BstBI TT'CG_AA
Cuts at: 4199 4199
Size: 5395
BstEII G'GTnAC_C
Cuts at: 1184 2047 1184
Size: 863 4532
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5395
BstYI r'GATC_y
Cuts at: 1972 2116 2503 2725 3092 3477 3494 4946 5032
Size: 144 387 222 367 385 17 1452 86
Cuts at: 5032 5044 5390 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 387 385 367 346 222 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2581 513
Size: 552 939 577 3327
Fragments arranged by size:
3327 939 577 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4656
Fragments arranged by size:
4656 683 56
BtsI GCAGTG_nn'
Cuts at: 2911 3705 3725 2911
Size: 794 20 4581
Fragments arranged by size:
4581 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2269 2304 529
Size: 35 1705 35 3620
Fragments arranged by size:
3620 1705 35 35
DraI TTT'AAA
Cuts at: 2699 2699
Size: 5395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4402 809
Size: 507 3086 1802
Fragments arranged by size:
3086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3731 5360
Size: 54 1425 50 275 18 1012 889 1629
Cuts at: 5360 8
Size: 43
Fragments arranged by size:
1629 1425 1012 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2842 1537
Size: 275 1030 4090
Fragments arranged by size:
4090 1030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 3325 1353
Size: 849 1123 3423
Fragments arranged by size:
3423 1123 849
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2575 3982 4356 4502 387
Size: 1064 138 986 1407 374 146 1280
Fragments arranged by size:
1407 1280 1064 986 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2393 2418 2438 3458 4055 4842
Size: 393 419 342 25 20 1020 597 787
Cuts at: 4842 5110 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 597 419 393 342 268
25 20
EcoRI G'AATT_C
Cuts at: 2704 3082 2704
Size: 378 5017
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2522 2356
Size: 166 5229
FspI TGC'GCA
Cuts at: 3901 3901
Size: 5395
HaeII r_GCGC'y
Cuts at: 1678 4542 1678
Size: 2864 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 4036 4068 4110
Size: 32 135 32 659 32 1773 32 42
Cuts at: 4110 4142 1373
Size: 32 2626
Fragments arranged by size:
2626 1773 659 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2015 2739 2985 669
Size: 988 281 77 724 246 3079
Fragments arranged by size:
3079 988 724 281 246 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5395
HpaI GTT'AAC
Cuts at: 2985 2985
Size: 5395
KpnI G_GTAC'C
Cuts at: 2141 2720 2141
Size: 579 4816
MfeI C'AATT_G
Cuts at: 2295 2994 2295
Size: 699 4696
MluI A'CGCG_T
Cuts at: 5271 5271
Size: 5395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4509 4693 1305
Size: 3204 184 2007
Fragments arranged by size:
3204 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3573
Fragments arranged by size:
3573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2532 2544 3353 3712 3871 518
Size: 648 1366 12 809 359 159 2042
Fragments arranged by size:
2042 1366 809 648 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2029 2811 3488 4636 4881 1606
Size: 423 782 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2581 513
Size: 552 939 577 3327
Fragments arranged by size:
3327 939 577 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5395
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5395
NotI GC'GGCC_GC
Cuts at: 2842 2842
Size: 5395
NspI r_CATG'y
Cuts at: 1132 1229 2753 4298 1132
Size: 97 1524 1545 2229
Fragments arranged by size:
2229 1545 1524 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5395
PciI A'CATG_T
Cuts at: 4294 4294
Size: 5395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5395
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5395
PmeI GTTT'AAAC
Cuts at: 2699 2699
Size: 5395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 4977 5009 3464
Size: 32 1481 32 3850
Fragments arranged by size:
3850 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5395
PsiI TTA'TAA
Cuts at: 2965 2965
Size: 5395
PstI C_TGCA'G
Cuts at: 830 2692 2747 830
Size: 1862 55 3478
Fragments arranged by size:
3478 1862 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5395
SalI G'TCGA_C
Cuts at: 1936 2737 1936
Size: 801 4594
SbfI CC_TGCA'GG
Cuts at: 2747 2747
Size: 5395
ScaI AGT'ACT
Cuts at: 3643 5355 3643
Size: 1712 3683
SfcI C'TryA_G
Cuts at: 826 1045 2688 2743 3878 4559 4750 826
Size: 219 1643 55 1135 681 191 1471
Fragments arranged by size:
1643 1471 1135 681 219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5395
SmaI CCC'GGG
Cuts at: 1872 2722 1872
Size: 850 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3499 4400 4662 820
Size: 197 926 1556 901 262 1553
Fragments arranged by size:
1556 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3186 493
Size: 2693 2702
SpeI A'CTAG_T
Cuts at: 152 2591 152
Size: 2439 2956
SphI G_CATG'C
Cuts at: 1132 2753 1132
Size: 1621 3774
SspI AAT'ATT
Cuts at: 5 52 3319 5
Size: 47 3267 2081
Fragments arranged by size:
3267 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5395
StyI C'CwwG_G
Cuts at: 513 1065 2004 2581 2834 513
Size: 552 939 577 253 3074
Fragments arranged by size:
3074 939 577 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 3439 3592 3777 4222 325
Size: 1255 1842 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3641 5353 96
Size: 274 80 33 51 1443 1664 1712 138
Fragments arranged by size:
1712 1664 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5355 5387 5355
Size: 32 5363
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5395
XbaI T'CTAG_A
Cuts at: 2731 2731
Size: 5395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5395
XmaI C'CCGG_G
Cuts at: 1870 2720 1870
Size: 850 4545
XmnI GAAnn'nnTTC
Cuts at: 2366 3524 2366
Size: 1158 4237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3203 276
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI