Restriction map for
pFN21AE3063
(Circular) (Six-base) MAPSORT of: pFN21AE3063.seq Check: 5236 from: 1 to: 5362
pFN21AE3063 5362 bp
With 182 enzymes: SgfI *
March 28, 2012 12:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2196 2705 1937
Size: 259 509 4594
Fragments arranged by size:
4594 509 259
Acc65I G'GTAC_C
Cuts at: 2683 2683
Size: 5362
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 4809 5077 1632
Size: 1793 1384 268 1917
Fragments arranged by size:
1917 1793 1384 268
AfeI AGC'GCT
Cuts at: 2252 2252
Size: 5362
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 2431 4261 5238 1203
Size: 1228 1830 977 1327
Fragments arranged by size:
1830 1327 1228 977
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 4091 4091
Size: 5362
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5362
AlwNI CAG_nnn'CTG
Cuts at: 2652 3105 4677 2652
Size: 453 1572 3337
Fragments arranged by size:
3337 1572 453
ApaLI G'TGCA_C
Cuts at: 3419 4575 3419
Size: 1156 4206
ApoI r'AATT_y
Cuts at: 1493 2671 2897 3049 5158 1493
Size: 1178 226 152 2109 1697
Fragments arranged by size:
2109 1697 1178 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 1870
Size: 73 744 4545
Fragments arranged by size:
4545 744 73
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 4138 618
Size: 325 217 627 896 1455 1842
Fragments arranged by size:
1842 1455 896 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2681 1391
Size: 1290 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 3769
Fragments arranged by size:
3769 971 622
BbvCI CC'TCA_GC
Cuts at: 2445 2445
Size: 5362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3553 3587 1903
Size: 34 1616 34 3678
Fragments arranged by size:
3678 1616 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3254 4464 3254
Size: 1210 4152
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3973 136
Size: 107 122 71 631 605 2301 1525
Fragments arranged by size:
2301 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2226 5357 2226
Size: 3131 2231
BlpI GC'TnA_GC
Cuts at: 2779 2779
Size: 5362
Bme1580I G_kGCm'C
Cuts at: 1790 3423 4579 1790
Size: 1633 1156 2573
Fragments arranged by size:
2573 1633 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 3093 4051
Size: 416 53 83 399 24 942 734 958
Cuts at: 4051 442
Size: 1753
Fragments arranged by size:
1753 958 942 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2441 2572 2616 4022 1239
Size: 798 404 131 44 1406 2579
Fragments arranged by size:
2579 1406 798 404 131 44
Bpu10I CC'TnA_GC
Cuts at: 2445 2593 2445
Size: 148 5214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3487 4352 4650 3487
Size: 865 298 4199
Fragments arranged by size:
4199 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4025 882
Size: 617 2526 2219
Fragments arranged by size:
2526 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3153 493
Size: 711 1949 2702
Fragments arranged by size:
2702 1949 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3169 3551 4186 275
Size: 53 83 186 936 1636 382 635 1451
Fragments arranged by size:
1636 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3795 4193 4467 4614 5025 3795
Size: 398 274 147 411 4132
Fragments arranged by size:
4132 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5137
Fragments arranged by size:
5137 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5362
BseYI C'CCAG_C
Cuts at: 2582 2640 2645 4565 5061 2582
Size: 58 5 1920 496 2883
Fragments arranged by size:
2883 1920 496 58 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 1163
Size: 573 627 4162
Fragments arranged by size:
4162 627 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2619 2812 3573 4601 1540
Size: 275 186 618 193 761 1028 2301
Fragments arranged by size:
2301 1028 761 618 275 193 186
BsiHKAI G_wGCw'C
Cuts at: 3423 3508 4579 3423
Size: 85 1071 4206
Fragments arranged by size:
4206 1071 85
BsmI GAATG_Cn'
Cuts at: 2873 2966 2873
Size: 93 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2681 3423 3508 4579 1391
Size: 399 891 742 85 1071 2174
Fragments arranged by size:
2174 1071 891 742 399 85
BspEI T'CCGG_A
Cuts at: 5025 5025
Size: 5362
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2515 2717 844
Size: 1671 202 3489
Fragments arranged by size:
3489 1671 202
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 1394
Size: 429 1426 3507
Fragments arranged by size:
3507 1426 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3857 4031 65
Size: 1183 2609 174 1396
Fragments arranged by size:
2609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4006 4193 1700
Size: 110 2196 187 2869
Fragments arranged by size:
2869 2196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 3422 4434 3422
Size: 1012 4350
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5362
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5362
BstYI r'GATC_y
Cuts at: 1972 2226 2692 3059 3444 3461 4913 4999 5011
Size: 254 466 367 385 17 1452 86 12
Cuts at: 5011 5357 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 466 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 2268 2289 2619 513
Size: 552 939 32 232 21 330 3256
Fragments arranged by size:
3256 939 552 330 232 32 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 3550
Fragments arranged by size:
3550 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2104 2878 3672 3692 2104
Size: 774 794 20 3774
Fragments arranged by size:
3774 794 774 20
ClaI AT'CG_AT
Cuts at: 1554 2175 1554
Size: 621 4741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5327
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4369 809
Size: 507 3053 1802
Fragments arranged by size:
3053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2616 2809
Size: 54 1425 50 275 18 622 164 193
Cuts at: 2809 3698 5327 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 622 275 193 164 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2616 2809 1537
Size: 275 804 193 4090
Fragments arranged by size:
4090 804 275 193
EarI CTCTTCn'nnn_
Cuts at: 1353 3292 1353
Size: 1939 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 4323 4469 387
Size: 1064 138 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2441 2572 2616 3425 4022 4809
Size: 393 405 404 131 44 809 597 787
Cuts at: 4809 5077 1239
Size: 268 1524
Fragments arranged by size:
1524 809 787 597 405 404 393 268
131 44
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2545 2131
Size: 414 4948
FspI TGC'GCA
Cuts at: 3868 3868
Size: 5362
HaeII r_GCGC'y
Cuts at: 1678 2254 2539 4509 1678
Size: 576 285 1970 2531
Fragments arranged by size:
2531 1970 576 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4003 4035 4077 4109 1373
Size: 32 135 32 2431 32 42 32 2626
Fragments arranged by size:
2626 2431 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 2952 669
Size: 988 281 768 246 3079
Fragments arranged by size:
3079 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5362
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2687 2687
Size: 5362
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4476 4660 1305
Size: 3171 184 2007
Fragments arranged by size:
3171 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3540
Fragments arranged by size:
3540 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2516 3320 3679 3838 518
Size: 648 1233 117 804 359 159 2042
Fragments arranged by size:
2042 1233 804 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2015 2240 2339 2652 2658 2778 3455 4603
Size: 409 225 99 313 6 120 677 1148
Cuts at: 4603 4848 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 409 313 245 225 120
99 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2289 513
Size: 552 939 285 3586
Fragments arranged by size:
3586 939 552 285
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5362
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2720 4265 1132
Size: 97 1491 1545 2229
Fragments arranged by size:
2229 1545 1491 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5362
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5362
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5362
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2226 2657 2714 830
Size: 1396 431 57 3478
Fragments arranged by size:
3478 1396 431 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2015 2652 2658 2015
Size: 637 6 4719
Fragments arranged by size:
4719 637 6
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 2164 3610 5322 2164
Size: 1446 1712 2204
Fragments arranged by size:
2204 1712 1446
SfcI C'TryA_G
Cuts at: 826 1045 2222 2653 2710 3845 4526 4717 826
Size: 219 1177 431 57 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 681 431 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2689 1872
Size: 817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3466 4367 4629 820
Size: 197 926 1523 901 262 1553
Fragments arranged by size:
1553 1523 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 3286 5
Size: 47 3234 2081
Fragments arranged by size:
3234 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5362
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2473 2502 2801 513
Size: 552 939 285 184 29 299 3074
Fragments arranged by size:
3074 939 552 299 285 184 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 3389 3406 3559 3744 4189 325
Size: 1255 459 1350 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 459 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2162 3608 5320
Size: 274 80 33 51 1443 185 1446 1712
Cuts at: 5320 96
Size: 138
Fragments arranged by size:
1712 1446 1443 274 185 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 5322 5354 2398
Size: 32 2892 32 2406
Fragments arranged by size:
2892 2406 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5362
XbaI T'CTAG_A
Cuts at: 2698 2698
Size: 5362
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5362
XmaI C'CCGG_G
Cuts at: 1870 2687 1870
Size: 817 4545
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI