Restriction map for
pFN21AE3057
(Circular) (Six-base) MAPSORT of: pFN21AE3057.seq Check: 9804 from: 1 to: 5341
pFN21AE3057 5341 bp
With 182 enzymes: SgfI *
March 28, 2012 12:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2220 2220
Size: 5341
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AfeI AGC'GCT
Cuts at: 2091 2453 2091
Size: 362 4979
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 2321 4172 2321
Size: 1851 3490
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaLI G'TGCA_C
Cuts at: 2207 3398 4554 2207
Size: 1191 1156 2994
Fragments arranged by size:
2994 1191 1156
ApoI r'AATT_y
Cuts at: 1493 2335 2650 2876 3028 5137 1493
Size: 842 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 842 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2662 4117 618
Size: 325 217 627 537 338 1455 1842
Fragments arranged by size:
1842 1455 627 537 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2660 1391
Size: 635 634 4072
Fragments arranged by size:
4072 635 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3952 136
Size: 107 122 71 631 605 337 1943 1525
Fragments arranged by size:
1943 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2211 3402 4558 1790
Size: 421 1191 1156 2573
Fragments arranged by size:
2573 1191 1156 421
BmgBI CAC'GTC
Cuts at: 1318 2191 2368 1318
Size: 873 177 4291
Fragments arranged by size:
4291 873 177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2387 4001 1239
Size: 818 330 1614 2579
Fragments arranged by size:
2579 1614 818 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 3148 3530 4165
Size: 53 83 186 936 1062 553 382 635
Cuts at: 4165 275
Size: 1451
Fragments arranged by size:
1451 1062 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 2321 2487 3774 4172 4446 4593 5004 2124
Size: 197 166 1287 398 274 147 411 2461
Fragments arranged by size:
2461 1287 411 398 274 197 166 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5341
BseYI C'CCAG_C
Cuts at: 2232 2623 4544 5040 2232
Size: 391 1921 496 2533
Fragments arranged by size:
2533 1921 496 391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2066 2211 2586 3402 3487 4558 2066
Size: 145 375 816 85 1071 2849
Fragments arranged by size:
2849 1071 816 375 145 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2066 2211 2586 2660 3402 3487
Size: 399 236 40 145 375 74 742 85
Cuts at: 3487 4558 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 236 145 85
74 40
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 2505 3230 4960 2505
Size: 725 1730 2886
Fragments arranged by size:
2886 1730 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2696 844
Size: 1376 476 3489
Fragments arranged by size:
3489 1376 476
BsrBI CCG'CTC
Cuts at: 1394 1823 2479 3228 1394
Size: 429 656 749 3507
Fragments arranged by size:
3507 749 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 3985 4172 1700
Size: 110 511 1664 187 2869
Fragments arranged by size:
2869 1664 511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336
Size: 699 367 385 17 1452 86 12 346
Cuts at: 5336 1972
Size: 1977
Fragments arranged by size:
1977 1452 699 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 513
Size: 552 939 96 3754
Fragments arranged by size:
3754 939 552 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 2857 3651 3671 2604
Size: 253 794 20 4274
Fragments arranged by size:
4274 794 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DraIII CAC_nnn'GTG
Cuts at: 2206 2206
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 2788 3677
Size: 54 1425 50 275 18 613 345 889
Cuts at: 3677 5306 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 613 345 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3271 1353
Size: 701 1217 3423
Fragments arranged by size:
3423 1217 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2432 3928 4302 4448 387
Size: 1064 138 843 1496 374 146 1280
Fragments arranged by size:
1496 1280 1064 843 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2387 3404 4001 4788 5056 1239
Size: 393 425 330 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 425 393 330 268
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 2093 2455 2486 4488 1678
Size: 415 362 31 2002 2531
Fragments arranged by size:
2531 2002 415 362 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2445 10
Size: 54 1425 343 613 2906
Fragments arranged by size:
2906 1425 613 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 3299 3658 3817 518
Size: 648 1413 720 359 159 2042
Fragments arranged by size:
2042 1413 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2413 2623 2757 3434 4582 4827 1606
Size: 496 311 210 134 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 496 311 245 210 134
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PacI TTA_AT'TAA
Cuts at: 2253 2253
Size: 5341
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PfoI T'CCnGG_A
Cuts at: 2130 2472 2130
Size: 342 4999
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2282 1613
Size: 669 4672
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2693 830
Size: 1863 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2413 2413
Size: 5341
SacII CC_GC'GG
Cuts at: 2103 2103
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5341
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SexAI A'CCwGG_T
Cuts at: 2388 2388
Size: 5341
SfcI C'TryA_G
Cuts at: 826 1045 2689 3824 4505 4696 826
Size: 219 1644 1135 681 191 1471
Fragments arranged by size:
1644 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 2321 1700
Size: 621 4720
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4346 4608 820
Size: 197 926 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 2780 513
Size: 552 939 324 452 3074
Fragments arranged by size:
3074 939 552 452 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 5301 5333 2078
Size: 32 3191 32 2086
Fragments arranged by size:
3191 2086 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 1316
Size: 1074 4267
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2386
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SfiI SfoI SrfI SwaI