Restriction map for
pFN21AE3038
(Circular) (Six-base) MAPSORT of: pFN21AE3038.seq Check: 9649 from: 1 to: 5224
pFN21AE3038 5224 bp
With 182 enzymes: SgfI *
March 28, 2012 13:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2967 4539 2967
Size: 1572 3652
ApaLI G'TGCA_C
Cuts at: 3281 4437 3281
Size: 1156 4068
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 1870
Size: 73 606 4545
Fragments arranged by size:
4545 606 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 2545 4000 618
Size: 325 217 627 521 237 1455 1842
Fragments arranged by size:
1842 1455 627 521 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2543 1391
Size: 1152 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 928
Size: 971 352 3901
Fragments arranged by size:
3901 971 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2074 2108 2332 2366 3415 3449 1903
Size: 34 137 34 224 34 1049 34 3678
Fragments arranged by size:
3678 1049 224 137 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BfrBI ATG'CAT
Cuts at: 2384 2384
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3835 136
Size: 107 122 71 631 605 2163 1525
Fragments arranged by size:
2163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2641 2641
Size: 5224
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3285 4441 1790
Size: 523 972 1156 2573
Fragments arranged by size:
2573 1156 972 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 2955 3913
Size: 416 53 83 399 24 837 701 958
Cuts at: 3913 442
Size: 1753
Fragments arranged by size:
1753 958 837 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3884 1239
Size: 2645 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3031 3413 4048 275
Size: 53 83 186 936 1498 382 635 1451
Fragments arranged by size:
1498 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4999
Fragments arranged by size:
4999 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 1186
Size: 838 4386
BseYI C'CCAG_C
Cuts at: 2206 2217 2427 4427 4923 2206
Size: 11 210 2000 496 2507
Fragments arranged by size:
2507 2000 496 210 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 1163
Size: 573 372 4279
Fragments arranged by size:
4279 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2104 2674 3435 4463 1540
Size: 275 186 103 570 761 1028 2301
Fragments arranged by size:
2301 1028 761 570 275 186 103
BsiHKAI G_wGCw'C
Cuts at: 3285 3370 4441 3285
Size: 85 1071 4068
Fragments arranged by size:
4068 1071 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
BsmBI CGTCTCn'nnnn_
Cuts at: 2398 2398
Size: 5224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2543 3285 3370 4441 1391
Size: 399 523 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 230 85
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2579 844
Size: 1735 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3111 1394
Size: 429 205 1083 3507
Fragments arranged by size:
3507 1083 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3868 4055 1700
Size: 110 2058 187 2869
Fragments arranged by size:
2869 2058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 2020 1925
Size: 95 5129
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 2458 1184
Size: 1274 3950
BstYI r'GATC_y
Cuts at: 1972 2507 2554 2921 3306 3323 4775 4861 4873
Size: 535 47 367 385 17 1452 86 12
Cuts at: 4873 5219 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 86 47
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4485
Fragments arranged by size:
4485 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 2740 3534 3554 2350
Size: 390 794 20 4020
Fragments arranged by size:
4020 794 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2671 3560
Size: 54 1425 50 275 18 312 529 889
Cuts at: 3560 5189 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 529 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3811 4185 4331 387
Size: 1064 138 2222 374 146 1280
Fragments arranged by size:
2222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3287 3884 4671 4939 1239
Size: 393 1655 597 787 268 1524
Fragments arranged by size:
1655 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2293 2293
Size: 5224
EcoO109I rG'GnC_Cy
Cuts at: 2203 2248 2203
Size: 45 5179
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 4371 1678
Size: 2693 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2395 2427 3865 3897 3939
Size: 32 135 32 823 32 1438 32 42
Cuts at: 3939 3971 1373
Size: 32 2626
Fragments arranged by size:
2626 1438 823 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 3182 3541 3700 518
Size: 648 1116 900 359 159 2042
Fragments arranged by size:
2042 1116 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2372 2427 2640 3317 4465 4710 1606
Size: 766 55 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 245 213 55
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NsiI A_TGCA'T
Cuts at: 2386 2386
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2582 4127 1132
Size: 97 1353 1545 2229
Fragments arranged by size:
2229 1545 1353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5224
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2213 1108
Size: 1105 4119
PfoI T'CCnGG_A
Cuts at: 2510 2510
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2248 2203
Size: 45 5179
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
PvuII CAG'CTG
Cuts at: 2427 2427
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SanDI GG'GwC_CC
Cuts at: 2203 2203
Size: 5224
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 2195 3472 5184 2195
Size: 1277 1712 2235
Fragments arranged by size:
2235 1712 1277
SfcI C'TryA_G
Cuts at: 826 1045 2572 3707 4388 4579 826
Size: 219 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2551 1872
Size: 679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3074
Fragments arranged by size:
3074 939 659 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3251 3268 3421 3606 4051 325
Size: 1255 1671 17 153 185 445 1498
Fragments arranged by size:
1671 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 3470 5182
Size: 274 80 33 51 1443 216 1277 1712
Cuts at: 5182 96
Size: 138
Fragments arranged by size:
1712 1443 1277 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2284 2316 5184 5216 2284
Size: 32 2868 32 2292
Fragments arranged by size:
2868 2292 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5224
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2214 2214
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2549 1870
Size: 679 4545
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI