Restriction map for
pFN21AE3037
(Circular) (Six-base) MAPSORT of: pFN21AE3037.seq Check: 3342 from: 1 to: 5212
pFN21AE3037 5212 bp
With 182 enzymes: SgfI *
March 28, 2012 12:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2533 2533
Size: 5212
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2257 3275 4659 4927 1632
Size: 625 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 625 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 2181 2332 4111 5088 1203
Size: 978 151 1779 977 1327
Fragments arranged by size:
1779 1327 978 977 151
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2955 4527 2955
Size: 1572 3640
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 1870
Size: 73 594 4545
Fragments arranged by size:
4545 594 73
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2533 3988 618
Size: 325 217 627 308 438 1455 1842
Fragments arranged by size:
1842 1455 627 438 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2531 1391
Size: 1140 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2358 2380 928
Size: 971 279 180 22 3760
Fragments arranged by size:
3760 971 279 180 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2239 3104 4314 2239
Size: 865 1210 3137
Fragments arranged by size:
3137 1210 865
BclI T'GATC_A
Cuts at: 1548 2152 1548
Size: 604 4608
BfrBI ATG'CAT
Cuts at: 2195 2195
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3823 136
Size: 107 122 71 631 605 2151 1525
Fragments arranged by size:
2151 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2629 2629
Size: 5212
Bme1580I G_kGCm'C
Cuts at: 1790 2098 3273 4429 1790
Size: 308 1175 1156 2573
Fragments arranged by size:
2573 1175 1156 308
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3901 442
Size: 416 53 83 399 24 1526 958 1753
Fragments arranged by size:
1753 1526 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3872 1239
Size: 2633 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3337 4202 4500 2333
Size: 1004 865 298 3045
Fragments arranged by size:
3045 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3875 882
Size: 617 2376 2219
Fragments arranged by size:
2376 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3019 3401 4036 275
Size: 53 83 186 936 1486 382 635 1451
Fragments arranged by size:
1486 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2479 1186
Size: 1293 3919
BseYI C'CCAG_C
Cuts at: 2025 4415 4911 2025
Size: 2390 496 2326
Fragments arranged by size:
2390 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4639
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2061 3273 3358 4429 2040
Size: 21 1212 85 1071 2823
Fragments arranged by size:
2823 1212 1071 85 21
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2061 2098 2531 3273 3358 4429
Size: 399 250 21 37 433 742 85 1071
Cuts at: 4429 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 433 399 250 85 37
21
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 844
Size: 1723 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 3881 65
Size: 1183 2459 174 1396
Fragments arranged by size:
2459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3856 4043 1700
Size: 110 2046 187 2869
Fragments arranged by size:
2869 2046 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5212
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2102 794
Size: 1308 3904
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2542 2909 3294 3311 4763 4849 4861 5207
Size: 570 367 385 17 1452 86 12 346
Cuts at: 5207 1972
Size: 1977
Fragments arranged by size:
1977 1452 570 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3721
Fragments arranged by size:
3721 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 2728 3522 3542 2476
Size: 252 794 20 4146
Fragments arranged by size:
4146 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3548 5177
Size: 54 1425 50 275 18 829 889 1629
Cuts at: 5177 8
Size: 43
Fragments arranged by size:
1629 1425 889 829 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2066 2493 3142 1353
Size: 699 14 427 649 3423
Fragments arranged by size:
3423 699 649 427 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 4173 4319 387
Size: 1064 138 2210 374 146 1280
Fragments arranged by size:
2210 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 3275 3872 4659 4927 1239
Size: 393 625 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 787 625 597 393 268
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 4359 1678
Size: 2681 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 3853 3885 3927
Size: 32 135 32 893 32 1356 32 42
Cuts at: 3927 3959 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 893 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2291 2556 2802 669
Size: 988 281 353 265 246 3079
Fragments arranged by size:
3079 988 353 281 265 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KpnI G_GTAC'C
Cuts at: 2537 2537
Size: 5212
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2250 2379 3170 3529 3688 518
Size: 648 1014 70 129 791 359 159 2042
Fragments arranged by size:
2042 1014 791 648 359 159 129 70
MspA1I CmG'CkG
Cuts at: 1606 2628 3305 4453 4698 1606
Size: 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3721
Fragments arranged by size:
3721 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NsiI A_TGCA'T
Cuts at: 2197 2197
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2185 2249 2570 4115 1132
Size: 97 956 64 321 1545 2229
Fragments arranged by size:
2229 1545 956 321 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 2181 4111 2181
Size: 1930 3282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5212
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2564 830
Size: 1734 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2066
Size: 5212
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SfcI C'TryA_G
Cuts at: 826 1045 2560 3695 4376 4567 826
Size: 219 1515 1135 681 191 1471
Fragments arranged by size:
1515 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5212
SmaI CCC'GGG
Cuts at: 1872 2539 1872
Size: 667 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 3316 4217 4479 820
Size: 197 926 369 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2249 2570 1132
Size: 1117 321 3774
Fragments arranged by size:
3774 1117 321
SspI AAT'ATT
Cuts at: 5 52 2438 3136 5
Size: 47 2386 698 2081
Fragments arranged by size:
2386 2081 698 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2651 513
Size: 552 939 647 3074
Fragments arranged by size:
3074 939 647 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3239 3256 3409 3594 4039 325
Size: 1255 525 1134 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1134 525 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2537 1870
Size: 667 4545
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI