Restriction map for
pFN21AE3036
(Circular) (Six-base) MAPSORT of: pFN21AE3036.seq Check: 7481 from: 1 to: 5212
pFN21AE3036 5212 bp
With 182 enzymes: SgfI *
March 28, 2012 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2271 2271
Size: 5212
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2533 2533
Size: 5212
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3275 4659 4927 1632
Size: 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 2179 4111 5088 1203
Size: 976 1932 977 1327
Fragments arranged by size:
1932 1327 977 976
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2955 4527 2955
Size: 1572 3640
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 1870
Size: 73 594 4545
Fragments arranged by size:
4545 594 73
BamHI G'GATC_C
Cuts at: 2491 2542 2909 2491
Size: 51 367 4794
Fragments arranged by size:
4794 367 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2533 3988 618
Size: 325 217 627 746 1455 1842
Fragments arranged by size:
1842 1455 746 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2531 1391
Size: 1140 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2175 928
Size: 971 276 3965
Fragments arranged by size:
3965 971 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2132 3104 4314 2132
Size: 972 1210 3030
Fragments arranged by size:
3030 1210 972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3823 136
Size: 107 122 71 631 605 2151 1525
Fragments arranged by size:
2151 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2629 2629
Size: 5212
Bme1580I G_kGCm'C
Cuts at: 1790 3273 4429 1790
Size: 1483 1156 2573
Fragments arranged by size:
2573 1483 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3901 442
Size: 416 53 83 399 24 1526 958 1753
Fragments arranged by size:
1753 1526 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3872 1239
Size: 2633 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 3337 4202 4500 2172
Size: 1165 865 298 2884
Fragments arranged by size:
2884 1165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3875 882
Size: 617 2376 2219
Fragments arranged by size:
2376 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2180 3003 493
Size: 711 976 823 2702
Fragments arranged by size:
2702 976 823 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3019 3401 4036 275
Size: 53 83 186 936 1486 382 635 1451
Fragments arranged by size:
1486 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 1186
Size: 846 4366
BseYI C'CCAG_C
Cuts at: 4415 4911 4415
Size: 496 4716
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 1163
Size: 573 395 4244
Fragments arranged by size:
4244 573 395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 3358 4429 3273
Size: 85 1071 4056
Fragments arranged by size:
4056 1071 85
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2531 3273 3358 4429 1391
Size: 399 741 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 399 85
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2567 844
Size: 1427 296 3489
Fragments arranged by size:
3489 1427 296
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 3881 65
Size: 1183 2459 174 1396
Fragments arranged by size:
2459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 3856 4043 1700
Size: 110 229 1817 187 2869
Fragments arranged by size:
2869 1817 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5212
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2491 2542 2909 3294 3311 4763 4849 4861
Size: 519 51 367 385 17 1452 86 12
Cuts at: 4861 5207 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 86 51
17 12
Bsu36I CC'TnA_GG
Cuts at: 2485 2485
Size: 5212
BtgI C'CryG_G
Cuts at: 513 1065 2010 513
Size: 552 945 3715
Fragments arranged by size:
3715 945 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3522 3542 2728
Size: 794 20 4398
Fragments arranged by size:
4398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3548 5177
Size: 54 1425 50 275 18 829 889 1629
Cuts at: 5177 8
Size: 43
Fragments arranged by size:
1629 1425 889 829 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3142 1353
Size: 1789 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 4173 4319 387
Size: 1064 138 2210 374 146 1280
Fragments arranged by size:
2210 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3275 3872 4659 4927 1239
Size: 393 1643 597 787 268 1524
Fragments arranged by size:
1643 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 5212
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 4359 1678
Size: 2681 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 3853 3885 3927
Size: 32 135 32 660 32 1589 32 42
Cuts at: 3927 3959 1373
Size: 32 2626
Fragments arranged by size:
2626 1589 660 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2802 669
Size: 988 281 618 246 3079
Fragments arranged by size:
3079 988 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KpnI G_GTAC'C
Cuts at: 2537 2537
Size: 5212
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3529 3688 518
Size: 648 2004 359 159 2042
Fragments arranged by size:
2042 2004 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2628 3305 4453 4698 1606
Size: 406 616 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 616 406 245
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 5212
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4660
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2570 4115 1132
Size: 97 1341 1545 2229
Fragments arranged by size:
2229 1545 1341 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5212
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PmlI CAC'GTG
Cuts at: 2180 2180
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5212
PshAI GACnn'nnGTC
Cuts at: 1613 2265 1613
Size: 652 4560
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2282 2429 2564 830
Size: 1452 147 135 3478
Fragments arranged by size:
3478 1452 147 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SanDI GG'GwC_CC
Cuts at: 2047 2047
Size: 5212
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SfcI C'TryA_G
Cuts at: 826 1045 2084 2278 2425 2560 3695 4376 4567
Size: 219 1039 194 147 135 1135 681 191
Cuts at: 4567 826
Size: 1471
Fragments arranged by size:
1471 1135 1039 681 219 194 191 147
135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5212
SmaI CCC'GGG
Cuts at: 1872 2539 1872
Size: 667 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 3316 4217 4479 820
Size: 197 926 244 1129 901 262 1553
Fragments arranged by size:
1553 1129 926 901 262 244 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2570 1132
Size: 1438 3774
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2651 513
Size: 552 1586 3074
Fragments arranged by size:
3074 1586 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3239 3256 3409 3594 4039 325
Size: 1255 1659 17 153 185 445 1498
Fragments arranged by size:
1659 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2099 3458 5170
Size: 274 80 33 51 1443 122 1359 1712
Cuts at: 5170 96
Size: 138
Fragments arranged by size:
1712 1443 1359 274 138 122 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2537 1870
Size: 667 4545
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI
XcmI