Restriction map for
pFN21AE3035
(Circular) (Six-base) MAPSORT of: pFN21AE3035.seq Check: 9472 from: 1 to: 5209
pFN21AE3035 5209 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3019 278
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2552 1937
Size: 615 4594
Acc65I G'GTAC_C
Cuts at: 2530 2530
Size: 5209
AclI AA'CG_TT
Cuts at: 1563 3336 3709 1563
Size: 1773 373 3063
Fragments arranged by size:
3063 1773 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 4656 4924 1632
Size: 1640 1384 268 1917
Fragments arranged by size:
1917 1640 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5012
AflIII A'CryG_T
Cuts at: 1203 4108 5085 1203
Size: 2905 977 1327
Fragments arranged by size:
2905 1327 977
AgeI A'CCGG_T
Cuts at: 4040 4040
Size: 5209
AhdI GACnn_n'nnGTC
Cuts at: 3938 3938
Size: 5209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5209
AlwNI CAG_nnn'CTG
Cuts at: 2236 2952 4524 2236
Size: 716 1572 2921
Fragments arranged by size:
2921 1572 716
ApaLI G'TGCA_C
Cuts at: 3266 4422 3266
Size: 1156 4053
ApoI r'AATT_y
Cuts at: 1493 2518 2744 2896 5005 1493
Size: 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3763 160
Size: 872 2731 1606
Fragments arranged by size:
2731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 2534 1870
Size: 73 465 126 4545
Fragments arranged by size:
4545 465 126 73
BamHI G'GATC_C
Cuts at: 2539 2906 2539
Size: 367 4842
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2530 3985 618
Size: 325 217 627 300 443 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 300 217
BanII G_rGCy'C
Cuts at: 1391 2409 2528 1391
Size: 1018 119 4072
Fragments arranged by size:
4072 1018 119
BbeI G_GCGC'C
Cuts at: 2091 2091
Size: 5209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3400 3434 1903
Size: 34 1463 34 3678
Fragments arranged by size:
3678 1463 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3101 4311 3101
Size: 1210 3999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2239 3820 136
Size: 107 122 71 631 605 567 1581 1525
Fragments arranged by size:
1581 1525 631 605 567 122 107 71
BglII A'GATC_T
Cuts at: 5204 5204
Size: 5209
BlpI GC'TnA_GC
Cuts at: 2626 2626
Size: 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2417 3270 4426 1790
Size: 627 853 1156 2573
Fragments arranged by size:
2573 1156 853 627
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5209
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2940 3898 442
Size: 416 53 83 399 24 1523 958 1753
Fragments arranged by size:
1753 1523 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 3869 1239
Size: 913 1717 2579
Fragments arranged by size:
2579 1717 913
Bpu10I CC'TnA_GC
Cuts at: 2489 2489
Size: 5209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2426 3334 4199 4497 2192
Size: 234 908 865 298 2904
Fragments arranged by size:
2904 908 865 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3872 882
Size: 617 2373 2219
Fragments arranged by size:
2373 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2332 3000 493
Size: 711 1128 668 2702
Fragments arranged by size:
2702 1128 711 668
BsaBI GATnn'nnATC
Cuts at: 5203 5203
Size: 5209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 3016 3398 4033
Size: 53 83 186 936 555 928 382 635
Cuts at: 4033 275
Size: 1451
Fragments arranged by size:
1451 936 928 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3642 4040 4314 4461 4872 3642
Size: 398 274 147 411 3979
Fragments arranged by size:
3979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2333 2363 2396 2426 1178
Size: 30 165 30 930 30 33 30 3961
Fragments arranged by size:
3961 930 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2357 2360 1186
Size: 1084 87 3 4035
Fragments arranged by size:
4035 1084 87 3
BseYI C'CCAG_C
Cuts at: 2379 4412 4908 2379
Size: 2033 496 2680
Fragments arranged by size:
2680 2033 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4636
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 2659 3420 4448 1540
Size: 275 186 80 578 761 1028 2301
Fragments arranged by size:
2301 1028 761 578 275 186 80
BsiHKAI G_wGCw'C
Cuts at: 2409 3270 3355 4426 2409
Size: 861 85 1071 3192
Fragments arranged by size:
3192 1071 861 85
BsmI GAATG_Cn'
Cuts at: 2720 2813 2720
Size: 93 5116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2417 2528 3270 3355 4426 1391
Size: 399 619 8 111 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 399 111 85 8
BspEI T'CCGG_A
Cuts at: 4872 4872
Size: 5209
BspHI T'CATG_A
Cuts at: 3098 4828 3098
Size: 1730 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2497 2564 844
Size: 1653 67 3489
Fragments arranged by size:
3489 1653 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3096 1394
Size: 429 1273 3507
Fragments arranged by size:
3507 1273 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3704 3878 65
Size: 1183 2456 174 1396
Fragments arranged by size:
2456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2076 2080 2469 3853 4040 1700
Size: 110 266 4 389 1384 187 2869
Fragments arranged by size:
2869 1384 389 266 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5209
BssSI C'ACGA_G
Cuts at: 2399 3269 4281 2399
Size: 870 1012 3327
Fragments arranged by size:
3327 1012 870
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2236 794
Size: 1442 3767
BstBI TT'CG_AA
Cuts at: 4013 4013
Size: 5209
BstEII G'GTnAC_C
Cuts at: 1184 2188 1184
Size: 1004 4205
BstYI r'GATC_y
Cuts at: 1972 2539 2906 3291 3308 4760 4846 4858 5204
Size: 567 367 385 17 1452 86 12 346
Cuts at: 5204 1972
Size: 1977
Fragments arranged by size:
1977 1452 567 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2324 513
Size: 552 1259 3398
Fragments arranged by size:
3398 1259 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4470
Fragments arranged by size:
4470 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 2725 3519 3539 2471
Size: 254 794 20 4141
Fragments arranged by size:
4141 794 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5174
DraI TTT'AAA
Cuts at: 2513 2513
Size: 5209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4216 809
Size: 507 2900 1802
Fragments arranged by size:
2900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2299 2656
Size: 54 1425 50 275 18 248 221 357
Cuts at: 2656 3545 5174 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 357 275 248 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2078 2656 1537
Size: 275 266 578 4090
Fragments arranged by size:
4090 578 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 3139 1353
Size: 1786 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 3796 4170 4316 387
Size: 1064 138 486 1721 374 146 1280
Fragments arranged by size:
1721 1280 1064 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2407 2407
Size: 5209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 3272 3869 4656 4924 1239
Size: 393 520 1120 597 787 268 1524
Fragments arranged by size:
1524 1120 787 597 520 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2179 2179
Size: 5209
EcoRI G'AATT_C
Cuts at: 2518 2896 2518
Size: 378 4831
FspI TGC'GCA
Cuts at: 3715 3715
Size: 5209
HaeII r_GCGC'y
Cuts at: 1678 2091 4356 1678
Size: 413 2265 2531
Fragments arranged by size:
2531 2265 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 3850 3882 3924
Size: 32 135 32 761 32 1485 32 42
Cuts at: 3924 3956 1373
Size: 32 2626
Fragments arranged by size:
2626 1485 761 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 2799 669
Size: 988 281 615 246 3079
Fragments arranged by size:
3079 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 2015 748
Size: 1267 3942
HpaI GTT'AAC
Cuts at: 2799 2799
Size: 5209
KasI G'GCGC_C
Cuts at: 2087 2087
Size: 5209
KpnI G_GTAC'C
Cuts at: 2534 2534
Size: 5209
MfeI C'AATT_G
Cuts at: 2808 2808
Size: 5209
MluI A'CGCG_T
Cuts at: 5085 5085
Size: 5209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2461 4323 4507 1305
Size: 1156 1862 184 2007
Fragments arranged by size:
2007 1862 1156 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3387
Fragments arranged by size:
3387 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3167 3526 3685 518
Size: 648 1314 687 359 159 2042
Fragments arranged by size:
2042 1314 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2326 2625 3302 4450 4695 1606
Size: 630 90 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 299 245 90
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5209
NarI GG'CG_CC
Cuts at: 2088 2088
Size: 5209
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4657
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5209
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5209
NotI GC'GGCC_GC
Cuts at: 2656 2656
Size: 5209
NspI r_CATG'y
Cuts at: 1132 1229 2479 2567 4112 1132
Size: 97 1250 88 1545 2229
Fragments arranged by size:
2229 1545 1250 97 88
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5209
PciI A'CATG_T
Cuts at: 4108 4108
Size: 5209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4307
PmeI GTTT'AAAC
Cuts at: 2513 2513
Size: 5209
PmlI CAC'GTG
Cuts at: 2332 2332
Size: 5209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 4791 4823 3278
Size: 32 1481 32 3664
Fragments arranged by size:
3664 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5209
PsiI TTA'TAA
Cuts at: 2779 2779
Size: 5209
PspXI vC'TCGA_Gb
Cuts at: 2408 2408
Size: 5209
PstI C_TGCA'G
Cuts at: 830 2561 830
Size: 1731 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5209
SacI G_AGCT'C
Cuts at: 2409 2409
Size: 5209
SacII CC_GC'GG
Cuts at: 2327 2327
Size: 5209
SalI G'TCGA_C
Cuts at: 1936 2551 1936
Size: 615 4594
SbfI CC_TGCA'GG
Cuts at: 2561 2561
Size: 5209
ScaI AGT'ACT
Cuts at: 3457 5169 3457
Size: 1712 3497
SfcI C'TryA_G
Cuts at: 826 1045 2557 3692 4373 4564 826
Size: 219 1512 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2089 2089
Size: 5209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5209
SmaI CCC'GGG
Cuts at: 1872 2536 1872
Size: 664 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 2408 2441 3313 4214 4476
Size: 197 926 264 201 33 872 901 262
Cuts at: 4476 820
Size: 1553
Fragments arranged by size:
1553 926 901 872 264 262 201 197
33
SnaBI TAC'GTA
Cuts at: 493 3000 493
Size: 2507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5209
SphI G_CATG'C
Cuts at: 1132 2567 1132
Size: 1435 3774
SspI AAT'ATT
Cuts at: 5 52 3133 5
Size: 47 3081 2081
Fragments arranged by size:
3081 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5209
StyI C'CwwG_G
Cuts at: 513 1065 2648 513
Size: 552 1583 3074
Fragments arranged by size:
3074 1583 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3236 3253 3406 3591 4036 325
Size: 1255 1656 17 153 185 445 1498
Fragments arranged by size:
1656 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3455 5167 96
Size: 274 80 33 51 1443 1478 1712 138
Fragments arranged by size:
1712 1478 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 5169 5201 2396
Size: 32 2741 32 2404
Fragments arranged by size:
2741 2404 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5209
XbaI T'CTAG_A
Cuts at: 2545 2545
Size: 5209
XhoI C'TCGA_G
Cuts at: 1943 2408 1943
Size: 465 4744
XmaI C'CCGG_G
Cuts at: 1870 2534 1870
Size: 664 4545
XmnI GAAnn'nnTTC
Cuts at: 3338 3338
Size: 5209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3017 276
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PpuMI PspOMI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI