Restriction map for
pFN21AE3033
(Circular) (Six-base) MAPSORT of: pFN21AE3033.seq Check: 8323 from: 1 to: 5158
pFN21AE3033 5158 bp
With 182 enzymes: SgfI *
March 28, 2012 13:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2968 278
Size: 53 83 186 936 1432 2468
Fragments arranged by size:
2468 1432 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2121 2501 1937
Size: 184 380 4594
Fragments arranged by size:
4594 380 184
Acc65I G'GTAC_C
Cuts at: 2141 2479 2141
Size: 338 4820
AclI AA'CG_TT
Cuts at: 1563 3285 3658 1563
Size: 1722 373 3063
Fragments arranged by size:
3063 1722 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3221 4605 4873 1632
Size: 1589 1384 268 1917
Fragments arranged by size:
1917 1589 1384 268
AfeI AGC'GCT
Cuts at: 2310 2310
Size: 5158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4961
AflIII A'CryG_T
Cuts at: 1203 2405 4057 5034 1203
Size: 1202 1652 977 1327
Fragments arranged by size:
1652 1327 1202 977
AgeI A'CCGG_T
Cuts at: 3989 3989
Size: 5158
AhdI GACnn_n'nnGTC
Cuts at: 3887 3887
Size: 5158
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5158
AlwNI CAG_nnn'CTG
Cuts at: 2901 4473 2901
Size: 1572 3586
ApaLI G'TGCA_C
Cuts at: 2133 3215 4371 2133
Size: 1082 1156 2920
Fragments arranged by size:
2920 1156 1082
ApoI r'AATT_y
Cuts at: 1493 2098 2162 2180 2467 2693 2845 4954 1493
Size: 605 64 18 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 605 287 226 152 64 18
AseI AT'TA_AT
Cuts at: 160 1032 3712 160
Size: 872 2680 1606
Fragments arranged by size:
2680 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5158
AvaI C'yCGr_G
Cuts at: 1870 1943 2483 1870
Size: 73 540 4545
Fragments arranged by size:
4545 540 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2442 2475 2442
Size: 33 5125
BamHI G'GATC_C
Cuts at: 2488 2855 2488
Size: 367 4791
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2479 3934 618
Size: 325 217 627 354 338 1455 1842
Fragments arranged by size:
1842 1455 627 354 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2477 1391
Size: 1086 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 928
Size: 971 402 3785
Fragments arranged by size:
3785 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3349 3383 1903
Size: 34 1412 34 3678
Fragments arranged by size:
3678 1412 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3050 4260 3050
Size: 1210 3948
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3769 136
Size: 107 122 71 631 605 2097 1525
Fragments arranged by size:
2097 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5153 5153
Size: 5158
BlpI GC'TnA_GC
Cuts at: 2575 2575
Size: 5158
Bme1580I G_kGCm'C
Cuts at: 1790 2137 3219 4375 1790
Size: 347 1082 1156 2573
Fragments arranged by size:
2573 1156 1082 347
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2889 3847 442
Size: 416 53 83 399 24 1472 958 1753
Fragments arranged by size:
1753 1472 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3818 1239
Size: 2579 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3283 4148 4446 3283
Size: 865 298 3995
Fragments arranged by size:
3995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3821 882
Size: 617 2322 2219
Fragments arranged by size:
2322 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2949 493
Size: 711 1745 2702
Fragments arranged by size:
2702 1745 711
BsaBI GATnn'nnATC
Cuts at: 5152 5152
Size: 5158
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2965 3347 3982
Size: 53 83 186 936 476 956 382 635
Cuts at: 3982 275
Size: 1451
Fragments arranged by size:
1451 956 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2380 3591 3989 4263 4410 4821 2380
Size: 1211 398 274 147 411 2717
Fragments arranged by size:
2717 1211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4933
Fragments arranged by size:
4933 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 1186
Size: 1013 4145
BseYI C'CCAG_C
Cuts at: 2305 4361 4857 2305
Size: 2056 496 2606
Fragments arranged by size:
2606 2056 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4585
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2608 3369 4397 1540
Size: 275 186 607 761 1028 2301
Fragments arranged by size:
2301 1028 761 607 275 186
BsiHKAI G_wGCw'C
Cuts at: 2137 3219 3304 4375 2137
Size: 1082 85 1071 2920
Fragments arranged by size:
2920 1082 1071 85
BsmI GAATG_Cn'
Cuts at: 2669 2762 2669
Size: 93 5065
BsmBI CGTCTCn'nnnn_
Cuts at: 2365 2365
Size: 5158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2477 3219 3304 4375 1391
Size: 399 347 340 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 340 85
BspEI T'CCGG_A
Cuts at: 4821 4821
Size: 5158
BspHI T'CATG_A
Cuts at: 2443 3047 4777 2443
Size: 604 1730 2824
Fragments arranged by size:
2824 1730 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2513 844
Size: 1669 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 1394
Size: 429 1222 3507
Fragments arranged by size:
3507 1222 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2269 3653 3827 65
Size: 1183 1021 1384 174 1396
Fragments arranged by size:
1396 1384 1183 1021 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3802 3989 1700
Size: 110 1992 187 2869
Fragments arranged by size:
2869 1992 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5158
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5158
BssSI C'ACGA_G
Cuts at: 3218 4230 3218
Size: 1012 4146
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5158
BstBI TT'CG_AA
Cuts at: 3962 3962
Size: 5158
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5158
BstYI r'GATC_y
Cuts at: 1972 2488 2855 3240 3257 4709 4795 4807 5153
Size: 516 367 385 17 1452 86 12 346
Cuts at: 5153 1972
Size: 1977
Fragments arranged by size:
1977 1452 516 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2211 2211
Size: 5158
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3667
Fragments arranged by size:
3667 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4419
Fragments arranged by size:
4419 683 56
BtsI GCAGTG_nn'
Cuts at: 2674 3468 3488 2674
Size: 794 20 4344
Fragments arranged by size:
4344 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5123
DraI TTT'AAA
Cuts at: 2179 2462 2179
Size: 283 4875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4165 809
Size: 507 2849 1802
Fragments arranged by size:
2849 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2605 3494 5123
Size: 54 1425 50 275 18 775 889 1629
Cuts at: 5123 8
Size: 43
Fragments arranged by size:
1629 1425 889 775 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2605 1537
Size: 275 793 4090
Fragments arranged by size:
4090 793 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 3088 1353
Size: 728 1007 3423
Fragments arranged by size:
3423 1007 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3745 4119 4265 387
Size: 1064 138 2156 374 146 1280
Fragments arranged by size:
2156 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3221 3818 4605 4873 1239
Size: 393 1589 597 787 268 1524
Fragments arranged by size:
1589 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2467 2845 2467
Size: 378 4780
EcoRV GAT'ATC
Cuts at: 2195 2195
Size: 5158
FspI TGC'GCA
Cuts at: 3664 3664
Size: 5158
HaeII r_GCGC'y
Cuts at: 1678 2312 4305 1678
Size: 634 1993 2531
Fragments arranged by size:
2531 1993 634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3799 3831 3873 3905 1373
Size: 32 135 32 2227 32 42 32 2626
Fragments arranged by size:
2626 2227 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2502 2748 669
Size: 988 281 564 246 3079
Fragments arranged by size:
3079 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5158
HpaI GTT'AAC
Cuts at: 2748 2748
Size: 5158
KpnI G_GTAC'C
Cuts at: 2145 2483 2145
Size: 338 4820
MfeI C'AATT_G
Cuts at: 2757 2757
Size: 5158
MluI A'CGCG_T
Cuts at: 5034 5034
Size: 5158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4272 4456 1305
Size: 2967 184 2007
Fragments arranged by size:
2967 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3336
Fragments arranged by size:
3336 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 3116 3475 3634 518
Size: 648 1084 866 359 159 2042
Fragments arranged by size:
2042 1084 866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2574 3251 4399 4644 1606
Size: 752 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 245 216
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3667
Fragments arranged by size:
3667 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5158
NotI GC'GGCC_GC
Cuts at: 2605 2605
Size: 5158
NspI r_CATG'y
Cuts at: 1132 1229 2350 2409 2516 4061 1132
Size: 97 1121 59 107 1545 2229
Fragments arranged by size:
2229 1545 1121 107 97 59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5158
PciI A'CATG_T
Cuts at: 2405 4057 2405
Size: 1652 3506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2066 1108
Size: 958 4200
PfoI T'CCnGG_A
Cuts at: 2065 2065
Size: 5158
PmeI GTTT'AAAC
Cuts at: 2462 2462
Size: 5158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 4740 4772 3227
Size: 32 1481 32 3613
Fragments arranged by size:
3613 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5158
PsiI TTA'TAA
Cuts at: 2728 2728
Size: 5158
PstI C_TGCA'G
Cuts at: 830 2510 830
Size: 1680 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5158
PvuII CAG'CTG
Cuts at: 2358 2358
Size: 5158
SalI G'TCGA_C
Cuts at: 1936 2500 1936
Size: 564 4594
SbfI CC_TGCA'GG
Cuts at: 2510 2510
Size: 5158
ScaI AGT'ACT
Cuts at: 3406 5118 3406
Size: 1712 3446
SexAI A'CCwGG_T
Cuts at: 2137 2137
Size: 5158
SfcI C'TryA_G
Cuts at: 826 1045 2027 2506 3641 4322 4513 826
Size: 219 982 479 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 681 479 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5158
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5158
SmaI CCC'GGG
Cuts at: 1872 2485 1872
Size: 613 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3262 4163 4425 820
Size: 197 926 1319 901 262 1553
Fragments arranged by size:
1553 1319 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2949 493
Size: 2456 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5158
SphI G_CATG'C
Cuts at: 1132 2350 2516 1132
Size: 1218 166 3774
Fragments arranged by size:
3774 1218 166
SspI AAT'ATT
Cuts at: 5 52 2236 3082 5
Size: 47 2184 846 2081
Fragments arranged by size:
2184 2081 846 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5158
StyI C'CwwG_G
Cuts at: 513 1065 2004 2597 513
Size: 552 939 593 3074
Fragments arranged by size:
3074 939 593 552
SwaI ATTT'AAAT
Cuts at: 2179 2179
Size: 5158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3185 3202 3355 3540 3985 325
Size: 1255 1605 17 153 185 445 1498
Fragments arranged by size:
1605 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3404 5116 96
Size: 274 80 33 51 1443 1427 1712 138
Fragments arranged by size:
1712 1443 1427 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5118 5150 5118
Size: 32 5126
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5158
XbaI T'CTAG_A
Cuts at: 2494 2494
Size: 5158
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5158
XmaI C'CCGG_G
Cuts at: 1870 2483 1870
Size: 613 4545
XmnI GAAnn'nnTTC
Cuts at: 2163 2235 3287 2163
Size: 72 1052 4034
Fragments arranged by size:
4034 1052 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2966 276
Size: 53 83 186 936 1432 2468
Fragments arranged by size:
2468 1432 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
XcmI