Restriction map for
pFN21AE3019
(Circular) (Six-base) MAPSORT of: pFN21AE3019.seq Check: 8685 from: 1 to: 5077
pFN21AE3019 5077 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2295 2295
Size: 5077
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2887 278
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2420 1937
Size: 483 4594
Acc65I G'GTAC_C
Cuts at: 2398 2398
Size: 5077
AclI AA'CG_TT
Cuts at: 1563 3204 3577 1563
Size: 1641 373 3063
Fragments arranged by size:
3063 1641 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3140 4524 4792 1632
Size: 1508 1384 268 1917
Fragments arranged by size:
1917 1508 1384 268
AfeI AGC'GCT
Cuts at: 2028 2186 2028
Size: 158 4919
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4880
AflIII A'CryG_T
Cuts at: 1203 3976 4953 1203
Size: 2773 977 1327
Fragments arranged by size:
2773 1327 977
AgeI A'CCGG_T
Cuts at: 3908 3908
Size: 5077
AhdI GACnn_n'nnGTC
Cuts at: 3806 3806
Size: 5077
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5077
AlwNI CAG_nnn'CTG
Cuts at: 2820 4392 2820
Size: 1572 3505
ApaI G_GGCC'C
Cuts at: 2158 2208 2158
Size: 50 5027
ApaLI G'TGCA_C
Cuts at: 3134 4290 3134
Size: 1156 3921
ApoI r'AATT_y
Cuts at: 1493 2386 2612 2764 4873 1493
Size: 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3631 160
Size: 872 2599 1606
Fragments arranged by size:
2599 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2110 2129 2222 2402 1870
Size: 73 144 23 19 93 180 4545
Fragments arranged by size:
4545 180 144 93 73 23 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2045 2078 2045
Size: 33 5044
BamHI G'GATC_C
Cuts at: 2407 2774 2407
Size: 367 4710
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 3853 618
Size: 325 217 627 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2129 2158 2208 2396 1391
Size: 696 42 29 50 188 4072
Fragments arranged by size:
4072 696 188 50 42 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2341 2375 3268 3302 1903
Size: 34 404 34 893 34 3678
Fragments arranged by size:
3678 893 404 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2969 4179 2969
Size: 1210 3867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5077
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3688 136
Size: 107 122 71 631 605 2016 1525
Fragments arranged by size:
2016 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5072 5072
Size: 5077
BlpI GC'TnA_GC
Cuts at: 2494 2494
Size: 5077
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2137 2158 2208 3138 4294 1790
Size: 276 71 21 50 930 1156 2573
Fragments arranged by size:
2573 1156 930 276 71 50 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 3766 442
Size: 416 53 83 399 24 1391 958 1753
Fragments arranged by size:
1753 1391 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2324 3737 1239
Size: 904 181 1413 2579
Fragments arranged by size:
2579 1413 904 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3202 4067 4365 3202
Size: 865 298 3914
Fragments arranged by size:
3914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3740 882
Size: 617 2241 2219
Fragments arranged by size:
2241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2868 493
Size: 711 1664 2702
Fragments arranged by size:
2702 1664 711
BsaBI GATnn'nnATC
Cuts at: 5071 5071
Size: 5077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2884 3266 3901 275
Size: 53 83 186 936 1351 382 635 1451
Fragments arranged by size:
1451 1351 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3510 3908 4182 4329 4740 3510
Size: 398 274 147 411 3847
Fragments arranged by size:
3847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4852
Fragments arranged by size:
4852 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2156 1186
Size: 909 61 4107
Fragments arranged by size:
4107 909 61
BseYI C'CCAG_C
Cuts at: 4280 4776 4280
Size: 496 4581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 2212 2243 2527 3288 4316
Size: 275 186 96 115 31 284 761 1028
Cuts at: 4316 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 284 275 186 115 96
31
BsiHKAI G_wGCw'C
Cuts at: 3138 3223 4294 3138
Size: 85 1071 3921
Fragments arranged by size:
3921 1071 85
BsmI GAATG_Cn'
Cuts at: 2588 2681 2588
Size: 93 4984
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2087 2129 2137 2158 2208 2396
Size: 399 276 21 42 8 21 50 188
Cuts at: 2396 3138 3223 4294 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 188 85 50
42 21 21 8
BspEI T'CCGG_A
Cuts at: 4740 4740
Size: 5077
BspHI T'CATG_A
Cuts at: 2966 4696 2966
Size: 1730 3347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 2432 844
Size: 1451 137 3489
Fragments arranged by size:
3489 1451 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2239 2964 1394
Size: 429 416 725 3507
Fragments arranged by size:
3507 725 429 416
BsrDI GCAATG_nn'
Cuts at: 65 1248 3572 3746 65
Size: 1183 2324 174 1396
Fragments arranged by size:
2324 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3721 3908 1700
Size: 110 1911 187 2869
Fragments arranged by size:
2869 1911 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5077
BssHII G'CGCG_C
Cuts at: 1925 2160 2335 1925
Size: 235 175 4667
Fragments arranged by size:
4667 235 175
BssSI C'ACGA_G
Cuts at: 3137 4149 3137
Size: 1012 4065
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5077
BstBI TT'CG_AA
Cuts at: 3881 3881
Size: 5077
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5077
BstYI r'GATC_y
Cuts at: 1972 2407 2774 3159 3176 4628 4714 4726 5072
Size: 435 367 385 17 1452 86 12 346
Cuts at: 5072 1972
Size: 1977
Fragments arranged by size:
1977 1452 435 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2243 513
Size: 552 1178 3347
Fragments arranged by size:
3347 1178 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4338
Fragments arranged by size:
4338 683 56
BtsI GCAGTG_nn'
Cuts at: 2593 3387 3407 2593
Size: 794 20 4263
Fragments arranged by size:
4263 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5042
DraI TTT'AAA
Cuts at: 2381 2381
Size: 5077
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4084 809
Size: 507 2768 1802
Fragments arranged by size:
2768 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2094 2209
Size: 54 1425 50 275 18 204 60 115
Cuts at: 2209 2240 2524 3413 5042 8
Size: 31 284 889 1629 43
Fragments arranged by size:
1629 1425 889 284 275 204 115 60
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2094 2209 2240 2524 1537
Size: 275 282 115 31 284 4090
Fragments arranged by size:
4090 284 282 275 115 31
EarI CTCTTCn'nnn_
Cuts at: 1353 3007 1353
Size: 1654 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 3664 4038 4184 387
Size: 1064 138 606 1469 374 146 1280
Fragments arranged by size:
1469 1280 1064 606 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2324 3140 3737 4524 4792 1239
Size: 393 511 181 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 511 393 268 181
EcoO109I rG'GnC_Cy
Cuts at: 2107 2154 2204 2107
Size: 47 50 4980
Fragments arranged by size:
4980 50 47
EcoRI G'AATT_C
Cuts at: 2386 2764 2386
Size: 378 4699
FspI TGC'GCA
Cuts at: 3583 3583
Size: 5077
HaeII r_GCGC'y
Cuts at: 1678 2030 2188 4224 1678
Size: 352 158 2036 2531
Fragments arranged by size:
2531 2036 352 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2205 2237 3718 3750 3792
Size: 32 135 32 633 32 1481 32 42
Cuts at: 3792 3824 1373
Size: 32 2626
Fragments arranged by size:
2626 1481 633 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 2667 669
Size: 988 281 483 246 3079
Fragments arranged by size:
3079 988 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5077
HpaI GTT'AAC
Cuts at: 2667 2667
Size: 5077
KpnI G_GTAC'C
Cuts at: 2402 2402
Size: 5077
MfeI C'AATT_G
Cuts at: 2676 2676
Size: 5077
MluI A'CGCG_T
Cuts at: 4953 4953
Size: 5077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4191 4375 1305
Size: 2886 184 2007
Fragments arranged by size:
2886 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3255
Fragments arranged by size:
3255 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3394 3553 518
Size: 648 1869 359 159 2042
Fragments arranged by size:
2042 1869 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2245 2329 2493 3170 4318 4563 1606
Size: 453 186 84 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 245 186 164 84
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4525
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5077
NotI GC'GGCC_GC
Cuts at: 2240 2524 2240
Size: 284 4793
NspI r_CATG'y
Cuts at: 1132 1229 2435 3980 1132
Size: 97 1206 1545 2229
Fragments arranged by size:
2229 1545 1206 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4359
PciI A'CATG_T
Cuts at: 3976 3976
Size: 5077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5077
PfoI T'CCnGG_A
Cuts at: 2103 2103
Size: 5077
PmeI GTTT'AAAC
Cuts at: 2381 2381
Size: 5077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 4659 4691 3146
Size: 32 1481 32 3532
Fragments arranged by size:
3532 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2107
Size: 5077
PshAI GACnn'nnGTC
Cuts at: 1613 2112 1613
Size: 499 4578
PsiI TTA'TAA
Cuts at: 2647 2647
Size: 5077
PspOMI G'GGCC_C
Cuts at: 2154 2204 2154
Size: 50 5027
PstI C_TGCA'G
Cuts at: 830 2429 830
Size: 1599 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5077
PvuII CAG'CTG
Cuts at: 2059 2059
Size: 5077
SacII CC_GC'GG
Cuts at: 2246 2246
Size: 5077
SalI G'TCGA_C
Cuts at: 1936 2419 1936
Size: 483 4594
SbfI CC_TGCA'GG
Cuts at: 2429 2429
Size: 5077
ScaI AGT'ACT
Cuts at: 3325 5037 3325
Size: 1712 3365
SfcI C'TryA_G
Cuts at: 826 1045 2425 3560 4241 4432 826
Size: 219 1380 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5077
SmaI CCC'GGG
Cuts at: 1872 2131 2404 1872
Size: 259 273 4545
Fragments arranged by size:
4545 273 259
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3181 4082 4344 820
Size: 197 926 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2868 493
Size: 2375 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5077
SphI G_CATG'C
Cuts at: 1132 2435 1132
Size: 1303 3774
SspI AAT'ATT
Cuts at: 5 52 3001 5
Size: 47 2949 2081
Fragments arranged by size:
2949 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5077
StyI C'CwwG_G
Cuts at: 513 1065 2516 513
Size: 552 1451 3074
Fragments arranged by size:
3074 1451 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 3121 3274 3459 3904 325
Size: 1255 1524 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3323 5035 96
Size: 274 80 33 51 1443 1346 1712 138
Fragments arranged by size:
1712 1443 1346 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 5037 5069 2131
Size: 32 2874 32 2139
Fragments arranged by size:
2874 2139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5077
XbaI T'CTAG_A
Cuts at: 2413 2413
Size: 5077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5077
XmaI C'CCGG_G
Cuts at: 1870 2129 2402 1870
Size: 259 273 4545
Fragments arranged by size:
4545 273 259
XmnI GAAnn'nnTTC
Cuts at: 3206 3206
Size: 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2885 276
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI