Restriction map for
pFN21AE3012
(Circular) (Six-base) MAPSORT of: pFN21AE3012.seq Check: 6247 from: 1 to: 5008
pFN21AE3012 5008 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2818 278
Size: 53 83 186 936 1282 2468
Fragments arranged by size:
2468 1282 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2351 1937
Size: 414 4594
Acc65I G'GTAC_C
Cuts at: 2329 2329
Size: 5008
AclI AA'CG_TT
Cuts at: 1563 2307 3135 3508 1563
Size: 744 828 373 3063
Fragments arranged by size:
3063 828 744 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 3071 4455 4723 1632
Size: 450 989 1384 268 1917
Fragments arranged by size:
1917 1384 989 450 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4811
AflIII A'CryG_T
Cuts at: 1203 3907 4884 1203
Size: 2704 977 1327
Fragments arranged by size:
2704 1327 977
AgeI A'CCGG_T
Cuts at: 3839 3839
Size: 5008
AhdI GACnn_n'nnGTC
Cuts at: 3737 3737
Size: 5008
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2112 1184
Size: 928 4080
AlwNI CAG_nnn'CTG
Cuts at: 2014 2751 4323 2014
Size: 737 1572 2699
Fragments arranged by size:
2699 1572 737
ApaI G_GGCC'C
Cuts at: 2159 2159
Size: 5008
ApaLI G'TGCA_C
Cuts at: 3065 4221 3065
Size: 1156 3852
ApoI r'AATT_y
Cuts at: 1493 2317 2543 2695 4804 1493
Size: 824 226 152 2109 1697
Fragments arranged by size:
2109 1697 824 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3562 160
Size: 872 2530 1606
Fragments arranged by size:
2530 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5008
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 1870
Size: 73 390 4545
Fragments arranged by size:
4545 390 73
BamHI G'GATC_C
Cuts at: 2338 2705 2338
Size: 367 4641
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 3784 618
Size: 325 217 627 542 1455 1842
Fragments arranged by size:
1842 1455 627 542 325 217
BanII G_rGCy'C
Cuts at: 1391 2159 2327 1391
Size: 768 168 4072
Fragments arranged by size:
4072 768 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4037
BbvCI CC'TCA_GC
Cuts at: 2295 2295
Size: 5008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3199 3233 1903
Size: 34 50 34 1178 34 3678
Fragments arranged by size:
3678 1178 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2900 4110 2900
Size: 1210 3798
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5008
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3619 136
Size: 107 122 71 631 605 1947 1525
Fragments arranged by size:
1947 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5003 5003
Size: 5008
BlpI GC'TnA_GC
Cuts at: 2425 2425
Size: 5008
Bme1580I G_kGCm'C
Cuts at: 1790 2159 3069 4225 1790
Size: 369 910 1156 2573
Fragments arranged by size:
2573 1156 910 369
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5008
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2739 3697 442
Size: 416 53 83 399 24 1322 958 1753
Fragments arranged by size:
1753 1322 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2165 2264 3668 1239
Size: 911 15 99 1404 2579
Fragments arranged by size:
2579 1404 911 99 15
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 5008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3133 3998 4296 3133
Size: 865 298 3845
Fragments arranged by size:
3845 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3671 882
Size: 617 2172 2219
Fragments arranged by size:
2219 2172 617
BsaAI yAC'GTr
Cuts at: 493 1204 2799 493
Size: 711 1595 2702
Fragments arranged by size:
2702 1595 711
BsaBI GATnn'nnATC
Cuts at: 5002 5002
Size: 5008
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2815 3197 3832 275
Size: 53 83 186 936 1282 382 635 1451
Fragments arranged by size:
1451 1282 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3441 3839 4113 4260 4671 3441
Size: 398 274 147 411 3778
Fragments arranged by size:
3778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4783
Fragments arranged by size:
4783 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5008
BseYI C'CCAG_C
Cuts at: 2085 2158 2182 4211 4707 2085
Size: 73 24 2029 496 2386
Fragments arranged by size:
2386 2029 496 73 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2284 2458 3219 4247 1540
Size: 275 186 283 174 761 1028 2301
Fragments arranged by size:
2301 1028 761 283 275 186 174
BsiHKAI G_wGCw'C
Cuts at: 3069 3154 4225 3069
Size: 85 1071 3852
Fragments arranged by size:
3852 1071 85
BsmI GAATG_Cn'
Cuts at: 2519 2612 2519
Size: 93 4915
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2327 3069 3154 4225 1391
Size: 399 369 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 168 85
BspEI T'CCGG_A
Cuts at: 4671 4671
Size: 5008
BspHI T'CATG_A
Cuts at: 2897 4627 2897
Size: 1730 3278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2363 844
Size: 1519 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2082 2895 1394
Size: 429 259 813 3507
Fragments arranged by size:
3507 813 429 259
BsrDI GCAATG_nn'
Cuts at: 65 1248 3503 3677 65
Size: 1183 2255 174 1396
Fragments arranged by size:
2255 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3652 3839 1700
Size: 110 1842 187 2869
Fragments arranged by size:
2869 1842 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5008
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5008
BssSI C'ACGA_G
Cuts at: 2207 3068 4080 2207
Size: 861 1012 3135
Fragments arranged by size:
3135 1012 861
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5008
BstBI TT'CG_AA
Cuts at: 3812 3812
Size: 5008
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5008
BstYI r'GATC_y
Cuts at: 1972 2338 2705 3090 3107 4559 4645 4657 5003
Size: 366 367 385 17 1452 86 12 346
Cuts at: 5003 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 366 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2104 2104
Size: 5008
BtgI C'CryG_G
Cuts at: 513 1065 2237 513
Size: 552 1172 3284
Fragments arranged by size:
3284 1172 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4269
Fragments arranged by size:
4269 683 56
BtsI GCAGTG_nn'
Cuts at: 2296 2524 3318 3338 2296
Size: 228 794 20 3966
Fragments arranged by size:
3966 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4973
DraI TTT'AAA
Cuts at: 2312 2312
Size: 5008
DraIII CAC_nnn'GTG
Cuts at: 2209 2209
Size: 5008
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2193 4015 809
Size: 507 877 1822 1802
Fragments arranged by size:
1822 1802 877 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2281 2455
Size: 54 1425 50 275 18 219 232 174
Cuts at: 2455 3344 4973 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 275 232 219 174 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2281 2455 1537
Size: 275 469 174 4090
Fragments arranged by size:
4090 469 275 174
EarI CTCTTCn'nnn_
Cuts at: 1353 2938 1353
Size: 1585 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3595 3969 4115 387
Size: 1064 138 2006 374 146 1280
Fragments arranged by size:
2006 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2150 2165 2264 3071 3668 4455
Size: 393 450 68 15 99 807 597 787
Cuts at: 4455 4723 1239
Size: 268 1524
Fragments arranged by size:
1524 807 787 597 450 393 268 99
68 15
EcoO109I rG'GnC_Cy
Cuts at: 2125 2155 2186 2195 2125
Size: 30 31 9 4938
Fragments arranged by size:
4938 31 30 9
EcoRI G'AATT_C
Cuts at: 2317 2695 2317
Size: 378 4630
FspI TGC'GCA
Cuts at: 3514 3514
Size: 5008
HaeII r_GCGC'y
Cuts at: 1678 4155 1678
Size: 2477 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3649 3681 3723 3755 1373
Size: 32 135 32 2077 32 42 32 2626
Fragments arranged by size:
2626 2077 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2352 2598 669
Size: 988 281 414 246 3079
Fragments arranged by size:
3079 988 414 281 246
HindIII A'AGCT_T
Cuts at: 748 2064 748
Size: 1316 3692
HpaI GTT'AAC
Cuts at: 2598 2598
Size: 5008
KpnI G_GTAC'C
Cuts at: 2333 2333
Size: 5008
MfeI C'AATT_G
Cuts at: 2607 2607
Size: 5008
MluI A'CGCG_T
Cuts at: 4884 4884
Size: 5008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4122 4306 1305
Size: 2817 184 2007
Fragments arranged by size:
2817 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3186
Fragments arranged by size:
3186 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2966 3325 3484 518
Size: 648 1800 359 159 2042
Fragments arranged by size:
2042 1800 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2424 3101 4249 4494 1606
Size: 576 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 245 242
NcoI C'CATG_G
Cuts at: 513 1065 2237 513
Size: 552 1172 3284
Fragments arranged by size:
3284 1172 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5008
NotI GC'GGCC_GC
Cuts at: 2455 2455
Size: 5008
NspI r_CATG'y
Cuts at: 1132 1229 2366 3911 1132
Size: 97 1137 1545 2229
Fragments arranged by size:
2229 1545 1137 97
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 4165
PciI A'CATG_T
Cuts at: 3907 3907
Size: 5008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5008
PmeI GTTT'AAAC
Cuts at: 2312 2312
Size: 5008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 4590 4622 3077
Size: 32 1481 32 3463
Fragments arranged by size:
3463 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2186 2195 2125
Size: 61 9 4938
Fragments arranged by size:
4938 61 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5008
PsiI TTA'TAA
Cuts at: 2578 2578
Size: 5008
PspOMI G'GGCC_C
Cuts at: 2155 2155
Size: 5008
PstI C_TGCA'G
Cuts at: 830 2360 830
Size: 1530 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5008
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5008
SalI G'TCGA_C
Cuts at: 1936 2350 1936
Size: 414 4594
SanDI GG'GwC_CC
Cuts at: 2186 2195 2186
Size: 9 4999
SbfI CC_TGCA'GG
Cuts at: 2360 2360
Size: 5008
ScaI AGT'ACT
Cuts at: 3256 4968 3256
Size: 1712 3296
SfcI C'TryA_G
Cuts at: 826 1045 2356 3491 4172 4363 826
Size: 219 1311 1135 681 191 1471
Fragments arranged by size:
1471 1311 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5008
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5008
SmaI CCC'GGG
Cuts at: 1872 2335 1872
Size: 463 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3112 4013 4275 820
Size: 197 926 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2799 493
Size: 2306 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5008
SphI G_CATG'C
Cuts at: 1132 2366 1132
Size: 1234 3774
SspI AAT'ATT
Cuts at: 5 52 2932 5
Size: 47 2880 2081
Fragments arranged by size:
2880 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5008
StyI C'CwwG_G
Cuts at: 513 1065 2237 2447 513
Size: 552 1172 210 3074
Fragments arranged by size:
3074 1172 552 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 3052 3205 3390 3835 325
Size: 1255 1455 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3254 4966 96
Size: 274 80 33 51 1443 1277 1712 138
Fragments arranged by size:
1712 1443 1277 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4968 5000 4968
Size: 32 4976
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5008
XbaI T'CTAG_A
Cuts at: 2344 2344
Size: 5008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 5008
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5008
XmaI C'CCGG_G
Cuts at: 1870 2333 1870
Size: 463 4545
XmnI GAAnn'nnTTC
Cuts at: 3137 3137
Size: 5008
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2816 276
Size: 53 83 186 936 1282 2468
Fragments arranged by size:
2468 1282 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI