Restriction map for
pFN21AE3010
(Circular) (Six-base) MAPSORT of: pFN21AE3010.seq Check: 7384 from: 1 to: 4996
pFN21AE3010 4996 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2806 278
Size: 53 83 186 936 1270 2468
Fragments arranged by size:
2468 1270 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 2339 1937
Size: 145 257 4594
Fragments arranged by size:
4594 257 145
Acc65I G'GTAC_C
Cuts at: 2317 2317
Size: 4996
AclI AA'CG_TT
Cuts at: 1563 2234 3123 3496 1563
Size: 671 889 373 3063
Fragments arranged by size:
3063 889 671 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3059 4443 4711 1632
Size: 1427 1384 268 1917
Fragments arranged by size:
1917 1427 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4799
AflIII A'CryG_T
Cuts at: 1203 3895 4872 1203
Size: 2692 977 1327
Fragments arranged by size:
2692 1327 977
AgeI A'CCGG_T
Cuts at: 3827 3827
Size: 4996
AhdI GACnn_n'nnGTC
Cuts at: 3725 3725
Size: 4996
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4996
AlwNI CAG_nnn'CTG
Cuts at: 2739 4311 2739
Size: 1572 3424
ApaI G_GGCC'C
Cuts at: 2285 2285
Size: 4996
ApaLI G'TGCA_C
Cuts at: 3053 4209 3053
Size: 1156 3840
ApoI r'AATT_y
Cuts at: 1493 2305 2531 2683 4792 1493
Size: 812 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3550 160
Size: 872 2518 1606
Fragments arranged by size:
2518 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4996
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2321 1870
Size: 73 159 219 4545
Fragments arranged by size:
4545 219 159 73
AvrII C'CTAG_G
Cuts at: 2068 2068
Size: 4996
BamHI G'GATC_C
Cuts at: 2326 2693 2326
Size: 367 4629
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 3772 618
Size: 325 217 627 530 1455 1842
Fragments arranged by size:
1842 1455 627 530 325 217
BanII G_rGCy'C
Cuts at: 1391 2285 2315 1391
Size: 894 30 4072
Fragments arranged by size:
4072 894 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 928
Size: 971 225 3800
Fragments arranged by size:
3800 971 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3187 3221 1903
Size: 34 1250 34 3678
Fragments arranged by size:
3678 1250 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 4098 2888
Size: 1210 3786
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3607 136
Size: 107 122 71 631 605 1935 1525
Fragments arranged by size:
1935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4991 4991
Size: 4996
BlpI GC'TnA_GC
Cuts at: 2413 2413
Size: 4996
Bme1580I G_kGCm'C
Cuts at: 1790 2285 3057 4213 1790
Size: 495 772 1156 2573
Fragments arranged by size:
2573 1156 772 495
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2727 3685 442
Size: 416 53 83 399 24 1310 958 1753
Fragments arranged by size:
1753 1310 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3656 1239
Size: 2417 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3121 3986 4284 3121
Size: 865 298 3833
Fragments arranged by size:
3833 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3659 882
Size: 617 2160 2219
Fragments arranged by size:
2219 2160 617
BsaAI yAC'GTr
Cuts at: 493 1204 2787 493
Size: 711 1583 2702
Fragments arranged by size:
2702 1583 711
BsaBI GATnn'nnATC
Cuts at: 4990 4990
Size: 4996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2121 2803 3185
Size: 53 83 186 936 552 36 682 382
Cuts at: 3185 3820 275
Size: 635 1451
Fragments arranged by size:
1451 936 682 635 552 382 186 83
53 36
BsaWI w'CCGG_w
Cuts at: 3429 3827 4101 4248 4659 3429
Size: 398 274 147 411 3766
Fragments arranged by size:
3766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 1178
Size: 30 165 30 735 30 4006
Fragments arranged by size:
4006 735 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 4035
BseYI C'CCAG_C
Cuts at: 4199 4695 4199
Size: 496 4500
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2276 2446 3207 4235 1540
Size: 275 186 275 170 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 275 186 170
BsiHKAI G_wGCw'C
Cuts at: 3057 3142 4213 3057
Size: 85 1071 3840
Fragments arranged by size:
3840 1071 85
BsmI GAATG_Cn'
Cuts at: 2507 2600 2507
Size: 93 4903
BsmBI CGTCTCn'nnnn_
Cuts at: 2199 2199
Size: 4996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2315 3057 3142 4213 1391
Size: 399 495 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 85 30
BspEI T'CCGG_A
Cuts at: 4659 4659
Size: 4996
BspHI T'CATG_A
Cuts at: 2885 4615 2885
Size: 1730 3266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2351 844
Size: 1507 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2883 1394
Size: 429 1060 3507
Fragments arranged by size:
3507 1060 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3491 3665 65
Size: 1183 2243 174 1396
Fragments arranged by size:
2243 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3640 3827 1700
Size: 110 1830 187 2869
Fragments arranged by size:
2869 1830 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4996
BssSI C'ACGA_G
Cuts at: 3056 4068 3056
Size: 1012 3984
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4996
BstBI TT'CG_AA
Cuts at: 3800 3800
Size: 4996
BstEII G'GTnAC_C
Cuts at: 1184 2179 1184
Size: 995 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 2292 2292
Size: 4996
BstYI r'GATC_y
Cuts at: 1972 2326 2693 3078 3095 4547 4633 4645 4991
Size: 354 367 385 17 1452 86 12 346
Cuts at: 4991 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 354 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 513
Size: 552 939 20 3485
Fragments arranged by size:
3485 939 552 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4257
Fragments arranged by size:
4257 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 3306 3326 2512
Size: 794 20 4182
Fragments arranged by size:
4182 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4961
DraI TTT'AAA
Cuts at: 2300 2300
Size: 4996
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2261 4003 809
Size: 507 945 1742 1802
Fragments arranged by size:
1802 1742 945 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 3332 4961
Size: 54 1425 50 275 18 613 889 1629
Cuts at: 4961 8
Size: 43
Fragments arranged by size:
1629 1425 889 613 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2443 1537
Size: 275 631 4090
Fragments arranged by size:
4090 631 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2926 1353
Size: 1573 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3583 3957 4103 387
Size: 1064 138 1994 374 146 1280
Fragments arranged by size:
1994 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3059 3656 4443 4711 1239
Size: 393 1427 597 787 268 1524
Fragments arranged by size:
1524 1427 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2281 2281
Size: 4996
EcoRI G'AATT_C
Cuts at: 2305 2683 2305
Size: 378 4618
FspI TGC'GCA
Cuts at: 2018 3502 2018
Size: 1484 3512
HaeII r_GCGC'y
Cuts at: 1678 2078 4143 1678
Size: 400 2065 2531
Fragments arranged by size:
2531 2065 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3637 3669 3711 3743 1373
Size: 32 135 32 2065 32 42 32 2626
Fragments arranged by size:
2626 2065 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2083 2340 2586 669
Size: 988 281 145 257 246 3079
Fragments arranged by size:
3079 988 281 257 246 145
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4996
HpaI GTT'AAC
Cuts at: 2586 2586
Size: 4996
KpnI G_GTAC'C
Cuts at: 2321 2321
Size: 4996
MfeI C'AATT_G
Cuts at: 2595 2595
Size: 4996
MluI A'CGCG_T
Cuts at: 4872 4872
Size: 4996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 4110 4294 1305
Size: 951 1854 184 2007
Fragments arranged by size:
2007 1854 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3174
Fragments arranged by size:
3174 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2954 3313 3472 518
Size: 648 1788 359 159 2042
Fragments arranged by size:
2042 1788 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2412 3089 4237 4482 1606
Size: 585 221 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 245 221
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3505
Fragments arranged by size:
3505 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4996
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4996
NotI GC'GGCC_GC
Cuts at: 2443 2443
Size: 4996
NspI r_CATG'y
Cuts at: 1132 1229 2354 3899 1132
Size: 97 1125 1545 2229
Fragments arranged by size:
2229 1545 1125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4996
PciI A'CATG_T
Cuts at: 3895 3895
Size: 4996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4996
PmeI GTTT'AAAC
Cuts at: 2300 2300
Size: 4996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 4578 4610 3065
Size: 32 1481 32 3451
Fragments arranged by size:
3451 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4996
PsiI TTA'TAA
Cuts at: 2566 2566
Size: 4996
PspOMI G'GGCC_C
Cuts at: 2281 2281
Size: 4996
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2132 2164 2132
Size: 32 4964
PstI C_TGCA'G
Cuts at: 830 2348 830
Size: 1518 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4996
SalI G'TCGA_C
Cuts at: 1936 2081 2338 1936
Size: 145 257 4594
Fragments arranged by size:
4594 257 145
SbfI CC_TGCA'GG
Cuts at: 2348 2348
Size: 4996
ScaI AGT'ACT
Cuts at: 3244 4956 3244
Size: 1712 3284
SexAI A'CCwGG_T
Cuts at: 2175 2175
Size: 4996
SfcI C'TryA_G
Cuts at: 826 1045 2344 3479 4160 4351 826
Size: 219 1299 1135 681 191 1471
Fragments arranged by size:
1471 1299 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4996
SmaI CCC'GGG
Cuts at: 1872 2323 1872
Size: 451 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3100 4001 4263 820
Size: 197 926 1157 901 262 1553
Fragments arranged by size:
1553 1157 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2787 493
Size: 2294 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4996
SphI G_CATG'C
Cuts at: 1132 2354 1132
Size: 1222 3774
SspI AAT'ATT
Cuts at: 5 52 2920 5
Size: 47 2868 2081
Fragments arranged by size:
2868 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4996
StyI C'CwwG_G
Cuts at: 513 1065 2004 2068 2435 513
Size: 552 939 64 367 3074
Fragments arranged by size:
3074 939 552 367 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3023 3040 3193 3378 3823 325
Size: 1255 1443 17 153 185 445 1498
Fragments arranged by size:
1498 1443 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3242 4954 96
Size: 274 80 33 51 1443 1265 1712 138
Fragments arranged by size:
1712 1443 1265 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4956 4988 4956
Size: 32 4964
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4996
XbaI T'CTAG_A
Cuts at: 2332 2332
Size: 4996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 4996
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4996
XmaI C'CCGG_G
Cuts at: 1870 2321 1870
Size: 451 4545
XmnI GAAnn'nnTTC
Cuts at: 3125 3125
Size: 4996
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2804 276
Size: 53 83 186 936 1270 2468
Fragments arranged by size:
2468 1270 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI