Restriction map for
pFN21AE3004
(Circular) (Six-base) MAPSORT of: pFN21AE3004.seq Check: 1816 from: 1 to: 4933
pFN21AE3004 4933 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2743 278
Size: 53 83 186 936 1207 2468
Fragments arranged by size:
2468 1207 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2276 1937
Size: 339 4594
Acc65I G'GTAC_C
Cuts at: 2254 2254
Size: 4933
AclI AA'CG_TT
Cuts at: 1563 3060 3433 1563
Size: 1497 373 3063
Fragments arranged by size:
3063 1497 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2996 4380 4648 1632
Size: 1364 1384 268 1917
Fragments arranged by size:
1917 1384 1364 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4736
AflIII A'CryG_T
Cuts at: 1203 3832 4809 1203
Size: 2629 977 1327
Fragments arranged by size:
2629 1327 977
AgeI A'CCGG_T
Cuts at: 3764 3764
Size: 4933
AhdI GACnn_n'nnGTC
Cuts at: 2058 3662 2058
Size: 1604 3329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4933
AlwNI CAG_nnn'CTG
Cuts at: 2099 2676 4248 2099
Size: 577 1572 2784
Fragments arranged by size:
2784 1572 577
ApaLI G'TGCA_C
Cuts at: 2990 4146 2990
Size: 1156 3777
ApoI r'AATT_y
Cuts at: 1493 2242 2468 2620 4729 1493
Size: 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 749 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3487 160
Size: 872 2455 1606
Fragments arranged by size:
2455 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4933
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2258 1870
Size: 73 201 114 4545
Fragments arranged by size:
4545 201 114 73
BamHI G'GATC_C
Cuts at: 2263 2630 2263
Size: 367 4566
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 3709 618
Size: 325 217 627 467 1455 1842
Fragments arranged by size:
1842 1455 627 467 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 1391
Size: 861 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3124 3158 1903
Size: 34 1187 34 3678
Fragments arranged by size:
3678 1187 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2825 4035 2825
Size: 1210 3723
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3544 136
Size: 107 122 71 631 605 1872 1525
Fragments arranged by size:
1872 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4928 4928
Size: 4933
BlpI GC'TnA_GC
Cuts at: 2350 2350
Size: 4933
Bme1580I G_kGCm'C
Cuts at: 1790 2994 4150 1790
Size: 1204 1156 2573
Fragments arranged by size:
2573 1204 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2664 3622 442
Size: 416 53 83 399 24 1247 958 1753
Fragments arranged by size:
1753 1247 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 3593 1239
Size: 903 1451 2579
Fragments arranged by size:
2579 1451 903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 3923 4221 3058
Size: 865 298 3770
Fragments arranged by size:
3770 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3596 882
Size: 617 2097 2219
Fragments arranged by size:
2219 2097 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 493
Size: 711 1520 2702
Fragments arranged by size:
2702 1520 711
BsaBI GATnn'nnATC
Cuts at: 4927 4927
Size: 4933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2740 3122 3757 275
Size: 53 83 186 936 1207 382 635 1451
Fragments arranged by size:
1451 1207 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2049 3366 3764 4038 4185 4596 2049
Size: 1317 398 274 147 411 2386
Fragments arranged by size:
2386 1317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2024 2033 2054 1178
Size: 30 165 30 600 21 9 21 4057
Fragments arranged by size:
4057 600 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4933
BseYI C'CCAG_C
Cuts at: 4136 4632 4136
Size: 496 4437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2383 3144 4172 1540
Size: 275 186 382 761 1028 2301
Fragments arranged by size:
2301 1028 761 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2994 3079 4150 2994
Size: 85 1071 3777
Fragments arranged by size:
3777 1071 85
BsmI GAATG_Cn'
Cuts at: 2071 2444 2537 2071
Size: 373 93 4467
Fragments arranged by size:
4467 373 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2994 3079 4150 1391
Size: 399 462 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 85
BspEI T'CCGG_A
Cuts at: 4596 4596
Size: 4933
BspHI T'CATG_A
Cuts at: 2822 4552 2822
Size: 1730 3203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 844
Size: 1444 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2820 1394
Size: 429 997 3507
Fragments arranged by size:
3507 997 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3428 3602 65
Size: 1183 2180 174 1396
Fragments arranged by size:
2180 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3577 3764 1700
Size: 110 1767 187 2869
Fragments arranged by size:
2869 1767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4933
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4933
BssSI C'ACGA_G
Cuts at: 2993 4005 2993
Size: 1012 3921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4933
BstBI TT'CG_AA
Cuts at: 3737 3737
Size: 4933
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4933
BstYI r'GATC_y
Cuts at: 1972 2263 2630 3015 3032 4484 4570 4582 4928
Size: 291 367 385 17 1452 86 12 346
Cuts at: 4928 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 291 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2229 487
Size: 683 56 1003 3191
Fragments arranged by size:
3191 1003 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 3243 3263 2449
Size: 794 20 4119
Fragments arranged by size:
4119 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4898
DraI TTT'AAA
Cuts at: 2237 2237
Size: 4933
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2227 3940 809
Size: 507 911 1713 1802
Fragments arranged by size:
1802 1713 911 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 3269 4898
Size: 54 1425 50 275 18 550 889 1629
Cuts at: 4898 8
Size: 43
Fragments arranged by size:
1629 1425 889 550 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2380 1537
Size: 275 568 4090
Fragments arranged by size:
4090 568 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2863 1353
Size: 1510 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3520 3894 4040 387
Size: 1064 138 1931 374 146 1280
Fragments arranged by size:
1931 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2996 3593 4380 4648 1239
Size: 393 510 854 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 510 393 268
EcoRI G'AATT_C
Cuts at: 2242 2620 2242
Size: 378 4555
FspI TGC'GCA
Cuts at: 3439 3439
Size: 4933
HaeII r_GCGC'y
Cuts at: 1678 2178 4080 1678
Size: 500 1902 2531
Fragments arranged by size:
2531 1902 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2024 2035 2044 2056
Size: 32 135 32 431 21 11 9 12
Cuts at: 2056 2076 3574 3606 3648 3680 1373
Size: 20 1498 32 42 32 2626
Fragments arranged by size:
2626 1498 431 135 42 32 32 32
32 21 20 12 11 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2277 2523 669
Size: 988 281 339 246 3079
Fragments arranged by size:
3079 988 339 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4933
HpaI GTT'AAC
Cuts at: 2523 2523
Size: 4933
KpnI G_GTAC'C
Cuts at: 2258 2258
Size: 4933
MfeI C'AATT_G
Cuts at: 2532 2532
Size: 4933
MluI A'CGCG_T
Cuts at: 4809 4809
Size: 4933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 4047 4231 1305
Size: 737 2005 184 2007
Fragments arranged by size:
2007 2005 737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3111
Fragments arranged by size:
3111 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2891 3250 3409 518
Size: 648 1725 359 159 2042
Fragments arranged by size:
2042 1725 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2349 3026 4174 4419 1606
Size: 743 677 1148 245 2120
Fragments arranged by size:
2120 1148 743 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4381
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4933
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4933
NotI GC'GGCC_GC
Cuts at: 2380 2380
Size: 4933
NspI r_CATG'y
Cuts at: 1132 1229 2291 3836 1132
Size: 97 1062 1545 2229
Fragments arranged by size:
2229 1545 1062 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4933
PciI A'CATG_T
Cuts at: 3832 3832
Size: 4933
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4933
PmeI GTTT'AAAC
Cuts at: 2237 2237
Size: 4933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 4515 4547 3002
Size: 32 1481 32 3388
Fragments arranged by size:
3388 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4933
PsiI TTA'TAA
Cuts at: 2503 2503
Size: 4933
PspXI vC'TCGA_Gb
Cuts at: 2144 2144
Size: 4933
PstI C_TGCA'G
Cuts at: 830 2285 830
Size: 1455 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4933
SalI G'TCGA_C
Cuts at: 1936 2275 1936
Size: 339 4594
SbfI CC_TGCA'GG
Cuts at: 2285 2285
Size: 4933
ScaI AGT'ACT
Cuts at: 3181 4893 3181
Size: 1712 3221
SfcI C'TryA_G
Cuts at: 826 1045 2281 3416 4097 4288 826
Size: 219 1236 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4933
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4933
SmaI CCC'GGG
Cuts at: 1872 2260 1872
Size: 388 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3037 3938 4200 820
Size: 197 926 201 893 901 262 1553
Fragments arranged by size:
1553 926 901 893 262 201 197
SnaBI TAC'GTA
Cuts at: 493 2724 493
Size: 2231 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4933
SphI G_CATG'C
Cuts at: 1132 2291 1132
Size: 1159 3774
SspI AAT'ATT
Cuts at: 5 52 2857 5
Size: 47 2805 2081
Fragments arranged by size:
2805 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4933
StyI C'CwwG_G
Cuts at: 513 1065 2372 513
Size: 552 1307 3074
Fragments arranged by size:
3074 1307 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2960 2977 3130 3315 3760 325
Size: 1255 1380 17 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3179 4891 96
Size: 274 80 33 51 1443 1202 1712 138
Fragments arranged by size:
1712 1443 1202 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4893 4925 4893
Size: 32 4901
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4933
XbaI T'CTAG_A
Cuts at: 2269 2269
Size: 4933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2213 2213
Size: 4933
XhoI C'TCGA_G
Cuts at: 1943 2144 1943
Size: 201 4732
XmaI C'CCGG_G
Cuts at: 1870 2258 1870
Size: 388 4545
XmnI GAAnn'nnTTC
Cuts at: 3062 3062
Size: 4933
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2741 276
Size: 53 83 186 936 1207 2468
Fragments arranged by size:
2468 1207 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI