Restriction map for
pFN21AE2798
(Circular) (Six-base) MAPSORT of: pFN21AE2798.seq Check: 677 from: 1 to: 6475
pFN21AE2798 6475 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3181 3181
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2472 3818 1937
Size: 535 1346 4594
Fragments arranged by size:
4594 1346 535
Acc65I G'GTAC_C
Cuts at: 3796 3796
Size: 6475
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4538 5922 6190 1632
Size: 2906 1384 268 1917
Fragments arranged by size:
2906 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 5374 6351 1203
Size: 4171 977 1327
Fragments arranged by size:
4171 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AlwNI CAG_nnn'CTG
Cuts at: 3637 4218 5790 3637
Size: 581 1572 4322
Fragments arranged by size:
4322 1572 581
ApaI G_GGCC'C
Cuts at: 2059 2635 2743 2905 2959 2059
Size: 576 108 162 54 5575
Fragments arranged by size:
5575 576 162 108 54
ApaLI G'TGCA_C
Cuts at: 4532 5688 4532
Size: 1156 5319
ApoI r'AATT_y
Cuts at: 1493 3784 4010 4162 6271 1493
Size: 2291 226 152 2109 1697
Fragments arranged by size:
2291 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 3800 1870
Size: 73 1857 4545
Fragments arranged by size:
4545 1857 73
BamHI G'GATC_C
Cuts at: 2685 3805 4172 2685
Size: 1120 367 4988
Fragments arranged by size:
4988 1120 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3548 3796 5251 618
Size: 325 217 627 1761 248 1455 1842
Fragments arranged by size:
1842 1761 1455 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2059 2274 2635 2743 2905 2959 3794 1391
Size: 668 215 361 108 162 54 835 4072
Fragments arranged by size:
4072 835 668 361 215 162 108 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3213 928
Size: 971 1314 4190
Fragments arranged by size:
4190 1314 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2462 2496 4666 4700 1903
Size: 34 50 34 441 34 2170 34 3678
Fragments arranged by size:
3678 2170 441 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2241 2435 3707
Size: 107 122 71 631 605 569 194 1272
Cuts at: 3707 5086 136
Size: 1379 1525
Fragments arranged by size:
1525 1379 1272 631 605 569 194 122
107 71
BglII A'GATC_T
Cuts at: 6470 6470
Size: 6475
BlpI GC'TnA_GC
Cuts at: 2123 3892 2123
Size: 1769 4706
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2635 2743 2905 2959 3553 4536 5692
Size: 269 576 108 162 54 594 983 1156
Cuts at: 5692 1790
Size: 2573
Fragments arranged by size:
2573 1156 983 594 576 269 162 108
54
BmgBI CAC'GTC
Cuts at: 1318 2018 1318
Size: 700 5775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3459 4206
Size: 416 53 83 399 24 643 1399 747
Cuts at: 4206 5164 442
Size: 958 1753
Fragments arranged by size:
1753 1399 958 747 643 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5135 1239
Size: 3896 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 2810 3026 5138 882
Size: 617 1149 162 216 2112 2219
Fragments arranged by size:
2219 2112 1149 617 216 162
BsaAI yAC'GTr
Cuts at: 493 1204 4266 493
Size: 711 3062 2702
Fragments arranged by size:
3062 2702 711
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 4282 4664 5299
Size: 53 83 186 936 729 2020 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
2020 1451 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6250
Fragments arranged by size:
6250 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3766 1186
Size: 2580 3895
BseYI C'CCAG_C
Cuts at: 2504 2558 2612 2990 3302 3464 5678 6174 2504
Size: 54 54 378 312 162 2214 496 2805
Fragments arranged by size:
2805 2214 496 378 312 162 54 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5902
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3925 4686 5714 1540
Size: 275 186 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4536 4621 5692 4536
Size: 85 1071 5319
Fragments arranged by size:
5319 1071 85
BsmI GAATG_Cn'
Cuts at: 3595 3986 4079 3595
Size: 391 93 5991
Fragments arranged by size:
5991 391 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2274 2635 2743 2905 2959 3553
Size: 399 269 215 361 108 162 54 594
Cuts at: 3553 3794 4536 4621 5692 1391
Size: 241 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 399 361 269 241
215 162 108 85 54
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3181 3830 844
Size: 2337 649 3489
Fragments arranged by size:
3489 2337 649
BsrBI CCG'CTC
Cuts at: 1394 1823 4362 1394
Size: 429 2539 3507
Fragments arranged by size:
3507 2539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2134 4970 5144 65
Size: 1183 886 2836 174 1396
Fragments arranged by size:
2836 1396 1183 886 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2240 2699 5119 5306 1700
Size: 110 430 459 2420 187 2869
Fragments arranged by size:
2869 2420 459 430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 3350 4535 5547 3350
Size: 1185 1012 4278
Fragments arranged by size:
4278 1185 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2861 3464 3563 2318
Size: 543 603 99 5230
Fragments arranged by size:
5230 603 543 99
BstYI r'GATC_y
Cuts at: 1972 2685 3659 3805 4172 4557 4574 6026 6112
Size: 713 974 146 367 385 17 1452 86
Cuts at: 6112 6124 6470 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 974 713 385 367 346 146
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2278 2311 2353 2638 2800 3016 3112
Size: 552 1213 33 42 285 162 216 96
Cuts at: 3112 3124 513
Size: 12 3864
Fragments arranged by size:
3864 1213 552 285 216 162 96 42
33 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4868
Fragments arranged by size:
4868 868 683 56
BtsI GCAGTG_nn'
Cuts at: 3991 4785 4805 3991
Size: 794 20 5661
Fragments arranged by size:
5661 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2261 3215 3431 5482 809
Size: 507 945 954 216 2051 1802
Fragments arranged by size:
2051 1802 954 945 507 216
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2350 2461
Size: 54 1425 50 275 18 243 277 111
Cuts at: 2461 2515 2569 2623 2677 2731 2785 2839 2893
Size: 54 54 54 54 54 54 54 54
Cuts at: 2893 2947 3001 3055 3109 3163 3271 3379 3487
Size: 54 54 54 54 54 108 108 108
Cuts at: 3487 3541 3565 3922 4811 6440 8
Size: 54 24 357 889 1629 43
Fragments arranged by size:
1629 1425 889 357 277 275 243 111
108 108 108 54 54 54 54 54
54 54 54 54 54 54 54 54
54 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3922 1537
Size: 275 2110 4090
Fragments arranged by size:
4090 2110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4405 1353
Size: 3052 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5062 5436 5582 387
Size: 1064 138 3473 374 146 1280
Fragments arranged by size:
3473 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4538 5135 5922 6190 1239
Size: 393 2906 597 787 268 1524
Fragments arranged by size:
2906 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3404 3404
Size: 6475
EcoO109I rG'GnC_Cy
Cuts at: 2055 2056 2152 2631 2739 2901 2955 3708 2055
Size: 1 96 479 108 162 54 753 4822
Fragments arranged by size:
4822 753 479 162 108 96 54 1
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3678 3678
Size: 6475
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 5622 1678
Size: 3944 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373
Size: 32 135 32 3544 32 42 32 2626
Fragments arranged by size:
3544 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 2473 3499 3607 3718 3819
Size: 988 281 424 111 1026 108 111 101
Cuts at: 3819 4065 669
Size: 246 3079
Fragments arranged by size:
3079 1026 988 424 281 246 111 111
108 101
HindIII A'AGCT_T
Cuts at: 748 2345 3536 748
Size: 1597 1191 3687
Fragments arranged by size:
3687 1597 1191
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KpnI G_GTAC'C
Cuts at: 3800 3800
Size: 6475
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 3361 5589 5773 1305
Size: 927 1129 2228 184 2007
Fragments arranged by size:
2228 2007 1129 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2352 2463 2517 2571 2625
Size: 54 1425 343 520 111 54 54 54
Cuts at: 2625 2679 2733 2787 2841 2895 2949 3003 3057
Size: 54 54 54 54 54 54 54 54
Cuts at: 3057 3165 3273 3381 3489 3543 3567 10
Size: 108 108 108 108 54 24 2918
Fragments arranged by size:
2918 1425 520 343 111 108 108 108
108 54 54 54 54 54 54 54
54 54 54 54 54 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 4433 4792 4951 518
Size: 648 3267 359 159 2042
Fragments arranged by size:
3267 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3634 3891 4568 5716 5961 1606
Size: 2028 257 677 1148 245 2120
Fragments arranged by size:
2120 2028 1148 677 257 245
NcoI C'CATG_G
Cuts at: 513 1065 2311 2353 2638 2800 3016 3112 3124
Size: 552 1246 42 285 162 216 96 12
Cuts at: 3124 513
Size: 3864
Fragments arranged by size:
3864 1246 552 285 216 162 96 42
12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2186 3833 5378 1132
Size: 97 957 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 957 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 1108
Size: 1176 5299
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PspOMI G'GGCC_C
Cuts at: 2055 2631 2739 2901 2955 2055
Size: 576 108 162 54 5575
Fragments arranged by size:
5575 576 162 108 54
PstI C_TGCA'G
Cuts at: 830 2485 2539 2593 3588 3827 830
Size: 1655 54 54 995 239 3478
Fragments arranged by size:
3478 1655 995 239 54 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 3634 3634
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 2471 3817 1936
Size: 535 1346 4594
Fragments arranged by size:
4594 1346 535
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2481 2535 2589 3584 3823 4958 5639
Size: 219 1436 54 54 995 239 1135 681
Cuts at: 5639 5830 826
Size: 191 1471
Fragments arranged by size:
1471 1436 1135 995 681 239 219 191
54 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 5480 5742 820
Size: 197 926 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 2186 3833 1132
Size: 1054 1647 3774
Fragments arranged by size:
3774 1647 1054
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 3140 3225 1517
Size: 1623 85 4767
Fragments arranged by size:
4767 1623 85
StyI C'CwwG_G
Cuts at: 513 1065 2311 2353 2638 2800 3016 3112 3124
Size: 552 1246 42 285 162 216 96 12
Cuts at: 3124 3220 3436 3490 3544 3914 513
Size: 96 216 54 54 370 3074
Fragments arranged by size:
3074 1246 552 370 285 216 216 162
96 96 54 54 42 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3703 4502 4519 4672 4857 5302 325
Size: 1255 2123 799 17 153 185 445 1498
Fragments arranged by size:
2123 1498 1255 799 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 3513 3545 6435 6467 2181
Size: 32 1300 32 2890 32 2189
Fragments arranged by size:
2890 2189 1300 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2858 3560 3638 2858
Size: 702 78 5695
Fragments arranged by size:
5695 702 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 4604 2207
Size: 2397 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI