Restriction map for
pFN21AE2796
(Circular) (Six-base) MAPSORT of: pFN21AE2796.seq Check: 2350 from: 1 to: 5917
pFN21AE2796 5917 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2484 2484
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 5364 5632 1632
Size: 2348 1384 268 1917
Fragments arranged by size:
2348 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2525 2566 4816 5793 1203
Size: 1322 41 2250 977 1327
Fragments arranged by size:
2250 1327 1322 977 41
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 2567 1166
Size: 1401 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3213 1184
Size: 2029 3888
AlwNI CAG_nnn'CTG
Cuts at: 2395 3660 5232 2395
Size: 1265 1572 3080
Fragments arranged by size:
3080 1572 1265
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 2642 3038 3226 3452 3604 5713 1493
Size: 1149 396 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1149 396 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2402 3242 1870
Size: 73 459 840 4545
Fragments arranged by size:
4545 840 459 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2544 3238 4693 618
Size: 325 217 627 678 79 694 1455 1842
Fragments arranged by size:
1842 1455 694 678 627 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2094 3236 1391
Size: 703 1142 4072
Fragments arranged by size:
4072 1142 703
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2989 3023 4108 4142 1903
Size: 34 1052 34 1085 34 3678
Fragments arranged by size:
3678 1085 1052 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2646 3809 5019 2646
Size: 1163 1210 3544
Fragments arranged by size:
3544 1210 1163
BclI T'GATC_A
Cuts at: 1548 3060 1548
Size: 1512 4405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 4528 136
Size: 107 122 71 631 605 355 2501 1525
Fragments arranged by size:
2501 1525 631 605 355 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2402 2549 3978 5134 1790
Size: 612 147 1429 1156 2573
Fragments arranged by size:
2573 1429 1156 612 147
BmgBI CAC'GTC
Cuts at: 1318 2526 1318
Size: 1208 4709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2836 3648 4606
Size: 416 53 83 399 24 1419 812 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1419 958 812 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4577 1239
Size: 3338 2579
Bpu10I CC'TnA_GC
Cuts at: 2331 2331
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 4042 4907 5205 2678
Size: 1364 865 298 3390
Fragments arranged by size:
3390 1364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2949 4580 882
Size: 617 1450 1631 2219
Fragments arranged by size:
2219 1631 1450 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 3708 493
Size: 711 1365 1139 2702
Fragments arranged by size:
2702 1365 1139 711
BsaBI GATnn'nnATC
Cuts at: 3065 5911 3065
Size: 2846 3071
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2295 4350 4748 5022 5169 5580 2295
Size: 2055 398 274 147 411 2632
Fragments arranged by size:
2632 2055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5917
BseYI C'CCAG_C
Cuts at: 5120 5616 5120
Size: 496 5421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3102 3367 4128 5156 1540
Size: 275 186 1101 265 761 1028 2301
Fragments arranged by size:
2301 1101 1028 761 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2910 3978 4063 5134 2070
Size: 840 1068 85 1071 2853
Fragments arranged by size:
2853 1071 1068 840 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2094 2402 2549 2910 3236 3978
Size: 399 280 24 308 147 361 326 742
Cuts at: 3978 4063 5134 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 326 308 280
147 85 24
BspEI T'CCGG_A
Cuts at: 2295 5580 2295
Size: 3285 2632
BspHI T'CATG_A
Cuts at: 2094 2355 3806 5536 2094
Size: 261 1451 1730 2475
Fragments arranged by size:
2475 1730 1451 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2484 2587 3272 844
Size: 1640 103 685 3489
Fragments arranged by size:
3489 1640 685 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 2462 4561 4748 1700
Size: 110 643 9 2099 187 2869
Fragments arranged by size:
2869 2099 643 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2298 2722 3247 3614 3999 4016 5468 5554
Size: 326 424 525 367 385 17 1452 86
Cuts at: 5554 5566 5912 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 525 424 385 367 346 326
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2229 2793 513
Size: 552 939 225 564 3637
Fragments arranged by size:
3637 939 564 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 3083 3433 4227 4247 3083
Size: 350 794 20 4753
Fragments arranged by size:
4753 794 350 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 2589 3221 2589
Size: 632 5285
DraIII CAC_nnn'GTG
Cuts at: 2427 2427
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2231 3119 4924 809
Size: 507 915 888 1805 1802
Fragments arranged by size:
1805 1802 915 888 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2790 3364 4253
Size: 54 1425 50 275 18 960 574 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 960 889 574 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 3847 1353
Size: 1001 1493 3423
Fragments arranged by size:
3423 1493 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2327 2870 4504 4878 5024 387
Size: 1064 138 738 543 1634 374 146 1280
Fragments arranged by size:
1634 1280 1064 738 543 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3980 4577 5364 5632 1239
Size: 393 2348 597 787 268 1524
Fragments arranged by size:
2348 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 5917
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2314 2315 2346 4558
Size: 32 135 32 711 31 1 31 2212
Cuts at: 4558 4590 4632 4664 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2212 711 135 42 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2824 3261 3507 669
Size: 988 281 886 437 246 3079
Fragments arranged by size:
3079 988 886 437 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 2792 3017 3875 4234 4393 518
Size: 648 1401 225 225 858 359 159 2042
Fragments arranged by size:
2042 1401 858 648 359 225 225 159
MspA1I CmG'CkG
Cuts at: 1606 2445 3333 4010 5158 5403 1606
Size: 839 888 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 839 677 245
NaeI GCC'GGC
Cuts at: 2455 2464 2455
Size: 9 5908
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4426
Fragments arranged by size:
4426 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2453 2462 2453
Size: 9 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 1108
Size: 1680 4237
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2569 2569
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2252 1613
Size: 639 5278
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2599 3269 830
Size: 1769 670 3478
Fragments arranged by size:
3478 1769 670
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2445 2445
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2948 4165 5877 2948
Size: 1217 1712 2988
Fragments arranged by size:
2988 1712 1217
SexAI A'CCwGG_T
Cuts at: 3213 3213
Size: 5917
SfcI C'TryA_G
Cuts at: 826 1045 2595 2950 3265 4400 5081 5272 826
Size: 219 1550 355 315 1135 681 191 1471
Fragments arranged by size:
1550 1471 1135 681 355 315 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2693 4021 4922 5184 820
Size: 197 926 750 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 750 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2993 1517
Size: 1476 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 2250 3356 513
Size: 552 939 246 1106 3074
Fragments arranged by size:
3074 1106 939 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 2483 3944 3961 4114 4299 4744
Size: 1255 599 304 1461 17 153 185 445
Cuts at: 4744 325
Size: 1498
Fragments arranged by size:
1498 1461 1255 599 445 304 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2447 2946 4163
Size: 274 80 33 51 1443 470 499 1217
Cuts at: 4163 5875 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1217 499 470 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI