Restriction map for
pFN21AE2795
(Circular) (Six-base) MAPSORT of: pFN21AE2795.seq Check: 3440 from: 1 to: 5896
pFN21AE2795 5896 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3239 1937
Size: 994 308 4594
Fragments arranged by size:
4594 994 308
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2827 2918 3959 5343 5611 1632
Size: 1195 91 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1195 1041 268 91
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 5896
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 2471 1166
Size: 1305 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2706 1184
Size: 1522 4374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 5864
AlwNI CAG_nnn'CTG
Cuts at: 2101 3639 5211 2101
Size: 1538 1572 2786
Fragments arranged by size:
2786 1572 1538
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 3205 3431 3583 5692 1493
Size: 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 2397 3226 3593 2397
Size: 829 367 4700
Fragments arranged by size:
4700 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2460 3217 4672 618
Size: 325 217 627 474 199 757 1455 1842
Fragments arranged by size:
1842 1455 757 627 474 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2204 3215 1391
Size: 813 1011 4072
Fragments arranged by size:
4072 1011 813
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2612 928
Size: 971 713 4212
Fragments arranged by size:
4212 971 713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2713 2747 2899 2933 4087 4121 1903
Size: 34 776 34 152 34 1154 34 3678
Fragments arranged by size:
3678 1154 776 152 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 2103 1548
Size: 555 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2699 4507 136
Size: 107 122 71 631 605 1027 1808 1525
Fragments arranged by size:
1808 1525 1027 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2465 3957 5113 1790
Size: 474 201 1492 1156 2573
Fragments arranged by size:
2573 1492 1156 474 201
BmgBI CAC'GTC
Cuts at: 1318 2665 2923 1318
Size: 1347 258 4291
Fragments arranged by size:
4291 1347 258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3119 3627 4585
Size: 416 53 83 399 24 1702 508 958
Cuts at: 4585 442
Size: 1753
Fragments arranged by size:
1753 1702 958 508 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4556 1239
Size: 3317 2579
Bpu10I CC'TnA_GC
Cuts at: 2376 2376
Size: 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 4021 4886 5184 3029
Size: 992 865 298 3741
Fragments arranged by size:
3741 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 4559 882
Size: 617 531 2529 2219
Fragments arranged by size:
2529 2219 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 2245 3687 493
Size: 711 1041 1442 2702
Fragments arranged by size:
2702 1442 1041 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2249 3703 4085 4720
Size: 53 83 186 936 716 1454 382 635
Cuts at: 4720 275
Size: 1451
Fragments arranged by size:
1454 1451 936 716 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2204 2436 4329 4727 5001 5148 5559 2204
Size: 232 1893 398 274 147 411 2541
Fragments arranged by size:
2541 1893 411 398 274 232 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3146 3176 1178
Size: 30 165 30 1743 30 3898
Fragments arranged by size:
3898 1743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 3095 1186
Size: 1165 744 3987
Fragments arranged by size:
3987 1165 744
BseYI C'CCAG_C
Cuts at: 2736 3134 5099 5595 2736
Size: 398 1965 496 3037
Fragments arranged by size:
3037 1965 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5323
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 3957 4042 5113 2436
Size: 1521 85 1071 3219
Fragments arranged by size:
3219 1521 1071 85
BsmI GAATG_Cn'
Cuts at: 2944 3407 3500 2944
Size: 463 93 5340
Fragments arranged by size:
5340 463 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2264 2436 2465 3215 3957 4042
Size: 399 414 60 172 29 750 742 85
Cuts at: 4042 5113 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 750 742 414 399 172 85
60 29
BspEI T'CCGG_A
Cuts at: 2204 2436 5559 2204
Size: 232 3123 2541
Fragments arranged by size:
3123 2541 232
BspHI T'CATG_A
Cuts at: 2496 3785 5515 2496
Size: 1289 1730 2877
Fragments arranged by size:
2877 1730 1289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2618 3251 844
Size: 1774 633 3489
Fragments arranged by size:
3489 1774 633
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4391 4565 65
Size: 1183 3143 174 1396
Fragments arranged by size:
3143 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 2702 1925
Size: 777 5119
BssSI C'ACGA_G
Cuts at: 2138 3956 4968 2138
Size: 1818 1012 3066
Fragments arranged by size:
3066 1818 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 2185 1184
Size: 1001 4895
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2473 2500 2050
Size: 423 27 5446
Fragments arranged by size:
5446 423 27
BstYI r'GATC_y
Cuts at: 1972 2397 2448 3226 3593 3978 3995 5447 5533
Size: 425 51 778 367 385 17 1452 86
Cuts at: 5533 5545 5891 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 778 425 385 367 346 86
51 17 12
BstZ17I GTA'TAC
Cuts at: 2932 2932
Size: 5896
Bsu36I CC'TnA_GG
Cuts at: 2256 2256
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 2466 513
Size: 552 1401 3943
Fragments arranged by size:
3943 1401 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3412 4206 4226 2884
Size: 528 794 20 4554
Fragments arranged by size:
4554 794 528 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2044 4903 809
Size: 507 728 2859 1802
Fragments arranged by size:
2859 1802 728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2407 3343 4232
Size: 54 1425 50 275 18 577 936 889
Cuts at: 4232 5861 8
Size: 1629 43
Fragments arranged by size:
1629 1425 936 889 577 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2558 3826 1353
Size: 701 504 1268 3423
Fragments arranged by size:
3423 1268 701 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2827 2918 3959 4556 5343 5611 1239
Size: 393 1195 91 1041 597 787 268 1524
Fragments arranged by size:
1524 1195 1041 787 597 393 268 91
EcoNI CCTnn'n_nnAGG
Cuts at: 2897 2897
Size: 5896
EcoO109I rG'GnC_Cy
Cuts at: 2632 3177 2632
Size: 545 5351
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 2383 5043 1678
Size: 705 2660 2531
Fragments arranged by size:
2660 2531 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 3146 3178 4537
Size: 32 135 32 667 32 875 32 1359
Cuts at: 4537 4569 4611 4643 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1359 875 667 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2043 5010 5194 1305
Size: 738 2967 184 2007
Fragments arranged by size:
2967 2007 738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4074
Fragments arranged by size:
4074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2288 2471 2498 3854 4213 4372
Size: 648 1065 57 183 27 1356 359 159
Cuts at: 4372 518
Size: 2042
Fragments arranged by size:
2042 1356 1065 648 359 183 159 57
27
MspA1I CmG'CkG
Cuts at: 1606 2349 2412 3152 3312 3989 5137 5382 1606
Size: 743 63 740 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 743 740 677 245 160 63
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5344
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 3254 4799 1132
Size: 97 2025 1545 2229
Fragments arranged by size:
2229 2025 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5896
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2443 3965 3997 5478 5510 2411
Size: 32 1522 32 1481 32 2797
Fragments arranged by size:
2797 1522 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5896
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 2858 4144 5856 2858
Size: 1286 1712 2898
Fragments arranged by size:
2898 1712 1286
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 5896
SfcI C'TryA_G
Cuts at: 826 1045 2422 3244 4379 5060 5251 826
Size: 219 1377 822 1135 681 191 1471
Fragments arranged by size:
1471 1377 1135 822 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3044 4000 4901 5163 820
Size: 197 926 1101 956 901 262 1553
Fragments arranged by size:
1553 1101 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 1517
Size: 1242 4654
StyI C'CwwG_G
Cuts at: 513 1065 2190 3335 513
Size: 552 1125 1145 3074
Fragments arranged by size:
3074 1145 1125 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2257 2728 3923 3940 4093 4278 4723
Size: 1255 677 471 1195 17 153 185 445
Cuts at: 4723 325
Size: 1498
Fragments arranged by size:
1498 1255 1195 677 471 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 3060 4142
Size: 274 80 33 51 1443 879 204 1082
Cuts at: 4142 5854 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1082 879 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5856 5888 5856
Size: 32 5864
Tth111I GACn'n_nGTC
Cuts at: 1316 2666 1316
Size: 1350 4546
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2224 2047
Size: 177 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI