Restriction map for
pFN21AE2788
(Circular) (Six-base) MAPSORT of: pFN21AE2788.seq Check: 4795 from: 1 to: 5608
pFN21AE2788 5608 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2071 2071
Size: 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2751 2951 1937
Size: 814 200 4594
Fragments arranged by size:
4594 814 200
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2492 3671 5055 5323 1632
Size: 555 305 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1179 555 305 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2722 3351 4923 2722
Size: 629 1572 3407
Fragments arranged by size:
3407 1572 629
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2110 2917 3143 3295 5404 1493
Size: 617 807 226 152 2109 1697
Fragments arranged by size:
2109 1697 807 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2415 4162 160
Size: 872 1383 1747 1606
Fragments arranged by size:
1747 1606 1383 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2929 4384 618
Size: 325 217 627 1142 1455 1842
Fragments arranged by size:
1842 1455 1142 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2927 1391
Size: 1536 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2756 928
Size: 971 857 3780
Fragments arranged by size:
3780 971 857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3799 3833 1903
Size: 34 50 34 1778 34 3678
Fragments arranged by size:
3678 1778 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 3669 4825 1790
Size: 1879 1156 2573
Fragments arranged by size:
2573 1879 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2517 3339 4297
Size: 416 53 83 399 24 1100 822 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1100 958 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2666 2810 4268 1239
Size: 838 589 144 1458 2579
Fragments arranged by size:
2579 1458 838 589 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 3733 4598 4896 2054
Size: 1679 865 298 2766
Fragments arranged by size:
2766 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2324 2786 4271 882
Size: 617 825 462 1485 2219
Fragments arranged by size:
2219 1485 825 617 462
BsaAI yAC'GTr
Cuts at: 493 1204 2170 3399 493
Size: 711 966 1229 2702
Fragments arranged by size:
2702 1229 966 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2726 3415 3797 4432
Size: 53 83 186 936 1193 689 382 635
Cuts at: 4432 275
Size: 1451
Fragments arranged by size:
1451 1193 936 689 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4677
BseYI C'CCAG_C
Cuts at: 2087 4811 5307 2087
Size: 2724 496 2388
Fragments arranged by size:
2724 2388 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 2209 3119 3212 2209
Size: 910 93 4605
Fragments arranged by size:
4605 910 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2927 3669 3754 4825 1391
Size: 399 1137 742 85 1071 2174
Fragments arranged by size:
2174 1137 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 2698 2739 3497 5227 2698
Size: 41 758 1730 3079
Fragments arranged by size:
3079 1730 758 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 2963 844
Size: 1227 892 3489
Fragments arranged by size:
3489 1227 892
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2902 4103 4277 65
Size: 1183 1654 1201 174 1396
Fragments arranged by size:
1654 1396 1201 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603
Size: 966 367 385 17 1452 86 12 346
Cuts at: 5603 1972
Size: 1977
Fragments arranged by size:
1977 1452 966 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3625
Fragments arranged by size:
3625 1431 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 3124 3918 3938 2634
Size: 490 794 20 4304
Fragments arranged by size:
4304 794 490 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2042 2077 529
Size: 35 1478 35 4060
Fragments arranged by size:
4060 1478 35 35
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 3055 3944
Size: 54 1425 50 275 18 579 646 889
Cuts at: 3944 5573 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 646 579 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2206 3538 1353
Size: 741 112 1332 3423
Fragments arranged by size:
3423 1332 741 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2187 2492 2666 2810 3671 4268
Size: 393 445 110 305 174 144 861 597
Cuts at: 4268 5055 5323 1239
Size: 787 268 1524
Fragments arranged by size:
1524 861 787 597 445 393 305 268
174 144 110
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2799 2831 4249
Size: 32 135 32 851 32 344 32 1418
Cuts at: 4249 4281 4323 4355 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1418 851 344 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 2952 3198 669
Size: 988 281 906 108 246 3079
Fragments arranged by size:
3079 988 906 281 246 108
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 2834 3207 2834
Size: 373 5235
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 3207
Fragments arranged by size:
3207 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 3024 3701 4849 5094 1606
Size: 945 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 945 677 473 245
NcoI C'CATG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3625
Fragments arranged by size:
3625 1431 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 2722 1108
Size: 1299 315 3994
Fragments arranged by size:
3994 1299 315
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 2073 2376 3178 2073
Size: 303 802 4503
Fragments arranged by size:
4503 802 303
PstI C_TGCA'G
Cuts at: 830 2259 2302 2960 830
Size: 1429 43 658 3478
Fragments arranged by size:
3478 1429 658 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 2039 3856 5568 2039
Size: 1817 1712 2079
Fragments arranged by size:
2079 1817 1712
SfcI C'TryA_G
Cuts at: 826 1045 2255 2298 2956 4091 4772 4963 826
Size: 219 1210 43 658 1135 681 191 1471
Fragments arranged by size:
1471 1210 1135 681 658 219 191 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3712 4613 4875 820
Size: 197 926 90 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2496 3047 513
Size: 552 1431 551 3074
Fragments arranged by size:
3074 1431 552 551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3635 3652 3805 3990 4435 325
Size: 1255 2055 17 153 185 445 1498
Fragments arranged by size:
2055 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2037 2676 3854
Size: 274 80 33 51 1443 60 639 1178
Cuts at: 3854 5566 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1178 639 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2774 2944 2774
Size: 170 5438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI