Restriction map for
pFN21AE2782
(Circular) (Six-base) MAPSORT of: pFN21AE2782.seq Check: 819 from: 1 to: 6055
pFN21AE2782 6055 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 3398 1937
Size: 412 1049 4594
Fragments arranged by size:
4594 1049 412
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2177 2713 2983 4118 5502 5770 1632
Size: 541 4 536 270 1135 1384 268 1917
Fragments arranged by size:
1917 1384 1135 541 536 270 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2630 3047 1166
Size: 1464 417 4174
Fragments arranged by size:
4174 1464 417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2711 1184
Size: 1527 4528
AlwNI CAG_nnn'CTG
Cuts at: 2044 3093 3312 3798 5370 2044
Size: 1049 219 486 1572 2729
Fragments arranged by size:
2729 1572 1049 486 219
ApaI G_GGCC'C
Cuts at: 2511 2639 2511
Size: 128 5927
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2027 3364 3590 3742 5851 1493
Size: 534 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 534 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 3380 1870
Size: 73 195 1242 4545
Fragments arranged by size:
4545 1242 195 73
BamHI G'GATC_C
Cuts at: 3081 3385 3752 3081
Size: 304 367 5384
Fragments arranged by size:
5384 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2492 3111 3376 4831
Size: 325 217 627 662 43 619 265 1455
Cuts at: 4831 618
Size: 1842
Fragments arranged by size:
1842 1455 662 627 619 325 265 217
43
BanII G_rGCy'C
Cuts at: 1391 2511 2639 3184 3215 3297 3374 1391
Size: 1120 128 545 31 82 77 4072
Fragments arranged by size:
4072 1120 545 128 82 77 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2287 2494 928
Size: 971 388 207 4489
Fragments arranged by size:
4489 971 388 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3119 3153 4246 4280 1903
Size: 34 1182 34 1093 34 3678
Fragments arranged by size:
3678 1182 1093 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2402 2774 4666
Size: 107 122 71 631 605 730 372 1892
Cuts at: 4666 136
Size: 1525
Fragments arranged by size:
1892 1525 730 631 605 372 122 107
71
BglII A'GATC_T
Cuts at: 2088 6050 2088
Size: 3962 2093
BlpI GC'TnA_GC
Cuts at: 2237 3296 3472 2237
Size: 1059 176 4820
Fragments arranged by size:
4820 1059 176
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2454 2495 2511 2639 3116 4116 5272
Size: 593 71 41 16 128 477 1000 1156
Cuts at: 5272 1790
Size: 2573
Fragments arranged by size:
2573 1156 1000 593 477 128 71 41
16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3322 3786 4744
Size: 416 53 83 399 24 1905 464 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1905 1753 958 464 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 4715 1239
Size: 913 2563 2579
Fragments arranged by size:
2579 2563 913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3049 3846 493
Size: 711 1845 797 2702
Fragments arranged by size:
2702 1845 797 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 3862 4244 4879
Size: 53 83 186 936 759 1570 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1570 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3063 4488 4886 5160 5307 5718 3063
Size: 1425 398 274 147 411 3400
Fragments arranged by size:
3400 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3197 3227 1178
Size: 30 165 30 1794 30 4006
Fragments arranged by size:
4006 1794 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2810 3263 1186
Size: 931 693 453 3978
Fragments arranged by size:
3978 931 693 453
BseYI C'CCAG_C
Cuts at: 2070 3300 5258 5754 2070
Size: 1230 1958 496 2371
Fragments arranged by size:
2371 1958 1230 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 5020
Fragments arranged by size:
5020 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3184 3297 4116 4201 5272 3184
Size: 113 819 85 1071 3967
Fragments arranged by size:
3967 1071 819 113 85
BsmI GAATG_Cn'
Cuts at: 2935 3566 3659 2935
Size: 631 93 5331
Fragments arranged by size:
5331 631 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3350 3350
Size: 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2383 2454 2495 2511 2639 3116 3184
Size: 399 593 71 41 16 128 477 68
Cuts at: 3184 3215 3297 3374 4116 4201 5272 1391
Size: 31 82 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 593 477 399 128 85
82 77 71 68 41 31 16
BspEI T'CCGG_A
Cuts at: 3063 5718 3063
Size: 2655 3400
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3410 844
Size: 2054 512 3489
Fragments arranged by size:
3489 2054 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2565 4699 4886 1700
Size: 110 755 2134 187 2869
Fragments arranged by size:
2869 2134 755 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 2255 4115 5127 2255
Size: 1860 1012 3183
Fragments arranged by size:
3183 1860 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2044 2371 794
Size: 1250 327 4478
Fragments arranged by size:
4478 1250 327
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 2672 2965 1184
Size: 1488 293 4274
Fragments arranged by size:
4274 1488 293
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 2632
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2088 3081 3333 3385 3752 4137 4154 5606
Size: 116 993 252 52 367 385 17 1452
Cuts at: 5606 5692 5704 6050 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 993 385 367 346 252 116
86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2763 513
Size: 552 939 168 591 3805
Fragments arranged by size:
3805 939 591 552 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3122 487
Size: 683 56 1896 3420
Fragments arranged by size:
3420 1896 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 3571 4365 4385 2412
Size: 1159 794 20 4082
Fragments arranged by size:
4082 1159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2154 2189 529
Size: 35 1590 35 4395
Fragments arranged by size:
4395 1590 35 35
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2766
Size: 54 1425 50 275 18 177 387 372
Cuts at: 2766 3186 3502 4391 6020 8
Size: 420 316 889 1629 43
Fragments arranged by size:
1629 1425 889 420 387 372 316 275
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 3985 1353
Size: 1298 1334 3423
Fragments arranged by size:
3423 1334 1298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2927 4642 5016 5162 387
Size: 1064 138 1338 1715 374 146 1280
Fragments arranged by size:
1715 1338 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3182 3295 3182
Size: 113 5942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2173 2177 2713 2983 4118 4715
Size: 393 520 21 4 536 270 1135 597
Cuts at: 4715 5502 5770 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1135 787 597 536 520 393 270
268 21 4
EcoO109I rG'GnC_Cy
Cuts at: 2077 2508 2636 2818 2946 2077
Size: 431 128 182 128 5186
Fragments arranged by size:
5186 431 182 128 128
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
EcoRV GAT'ATC
Cuts at: 2517 2517
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2985 3073 5202 1678
Size: 1307 88 2129 2531
Fragments arranged by size:
2531 2129 1307 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3318 3350 4696 4728 4770
Size: 32 135 32 1746 32 1346 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1746 1346 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2396 3188 10
Size: 54 1425 343 177 387 792 2877
Fragments arranged by size:
2877 1425 792 387 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3047 4013 4372 4531 518
Size: 648 1464 417 966 359 159 2042
Fragments arranged by size:
2042 1464 966 648 417 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 3300 3471 4148 5296 5541 1606
Size: 606 1088 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 1088 677 606 245 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2165 3059 3413 4958 1132
Size: 97 936 894 354 1545 2229
Fragments arranged by size:
2229 1545 936 894 354 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3312 1108
Size: 2204 3851
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2818 2946 2818
Size: 128 5927
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2507 2635 2507
Size: 128 5927
PstI C_TGCA'G
Cuts at: 830 2577 3407 830
Size: 1747 830 3478
Fragments arranged by size:
3478 1747 830
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2212 3300 2212
Size: 1088 4967
SacI G_AGCT'C
Cuts at: 3184 3297 3184
Size: 113 5942
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2573 3403 4538 5219 5410 826
Size: 219 1528 830 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 830 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4159 5060 5322 820
Size: 197 926 2216 901 262 1553
Fragments arranged by size:
2216 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 2165 3413 1132
Size: 1033 1248 3774
Fragments arranged by size:
3774 1248 1033
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 3494 513
Size: 552 939 541 949 3074
Fragments arranged by size:
3074 949 939 552 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2847 4082 4099 4252 4437 4882
Size: 1255 741 526 1235 17 153 185 445
Cuts at: 4882 325
Size: 1498
Fragments arranged by size:
1498 1255 1235 741 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4301 6013 96
Size: 274 80 33 51 1443 2324 1712 138
Fragments arranged by size:
2324 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 6015 6047 3073
Size: 32 2910 32 3081
Fragments arranged by size:
3081 2910 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2827 1316
Size: 1511 4544
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 3196 2770
Size: 426 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI