Restriction map for
pFN21AE2777
(Circular) (Six-base) MAPSORT of: pFN21AE2777.seq Check: 1101 from: 1 to: 7213
pFN21AE2777 7213 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5023 278
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4556 1937
Size: 2619 4594
Acc65I G'GTAC_C
Cuts at: 2705 4534 2705
Size: 1829 5384
AclI AA'CG_TT
Cuts at: 1563 5340 5713 1563
Size: 3777 373 3063
Fragments arranged by size:
3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 4055 4110 4188 4352 4412 5276 6660
Size: 526 1897 55 78 164 60 864 1384
Cuts at: 6660 6928 1632
Size: 268 1917
Fragments arranged by size:
1917 1897 1384 864 526 268 164 78
60 55
AfeI AGC'GCT
Cuts at: 2348 3527 2348
Size: 1179 6034
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7016
AflIII A'CryG_T
Cuts at: 1203 6112 7089 1203
Size: 4909 977 1327
Fragments arranged by size:
4909 1327 977
AgeI A'CCGG_T
Cuts at: 6044 6044
Size: 7213
AhdI GACnn_n'nnGTC
Cuts at: 5942 5942
Size: 7213
AleI CACnn'nnGTG
Cuts at: 1166 4442 1166
Size: 3276 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7213
AlwNI CAG_nnn'CTG
Cuts at: 4277 4956 6528 4277
Size: 679 1572 4962
Fragments arranged by size:
4962 1572 679
ApaLI G'TGCA_C
Cuts at: 5270 6426 5270
Size: 1156 6057
ApoI r'AATT_y
Cuts at: 1493 3412 3654 4133 4370 4522 4748 4900 7009
Size: 1919 242 479 237 152 226 152 2109
Cuts at: 7009 1493
Size: 1697
Fragments arranged by size:
2109 1919 1697 479 242 237 226 152
152
AseI AT'TA_AT
Cuts at: 160 1032 2514 3284 3944 5767 160
Size: 872 1482 770 660 1823 1606
Fragments arranged by size:
1823 1606 1482 872 770 660
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
AvaI C'yCGr_G
Cuts at: 1870 1943 4538 1870
Size: 73 2595 4545
Fragments arranged by size:
4545 2595 73
BamHI G'GATC_C
Cuts at: 2393 4543 4910 2393
Size: 2150 367 4696
Fragments arranged by size:
4696 2150 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2705 3223 4534 5989
Size: 325 217 627 369 549 518 1311 1455
Cuts at: 5989 618
Size: 1842
Fragments arranged by size:
1842 1455 1311 627 549 518 369 325
217
BanII G_rGCy'C
Cuts at: 1391 3576 4532 1391
Size: 2185 956 4072
Fragments arranged by size:
4072 2185 956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 4061 4492 928
Size: 971 1474 688 431 3649
Fragments arranged by size:
3649 1474 971 688 431
BbvCI CC'TCA_GC
Cuts at: 3542 3542
Size: 7213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5404 5438 1903
Size: 34 3467 34 3678
Fragments arranged by size:
3678 3467 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5105 6315 5105
Size: 1210 6003
BclI T'GATC_A
Cuts at: 1548 3088 3916 1548
Size: 1540 828 4845
Fragments arranged by size:
4845 1540 828
BfrBI ATG'CAT
Cuts at: 3239 3420 3239
Size: 181 7032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5824 136
Size: 107 122 71 631 605 4152 1525
Fragments arranged by size:
4152 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 7208 2085
Size: 5123 2090
BlpI GC'TnA_GC
Cuts at: 4630 4630
Size: 7213
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2159 5274 6430 1790
Size: 350 19 3115 1156 2573
Fragments arranged by size:
3115 2573 1156 350 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4944 5902 442
Size: 416 53 83 399 24 3527 958 1753
Fragments arranged by size:
3527 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3609 4326 5873 1239
Size: 1676 694 717 1547 2579
Fragments arranged by size:
2579 1676 1547 717 694
Bpu10I CC'TnA_GC
Cuts at: 3542 3542
Size: 7213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5338 6203 6501 5338
Size: 865 298 6050
Fragments arranged by size:
6050 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5876 882
Size: 617 4377 2219
Fragments arranged by size:
4377 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5004 493
Size: 711 3800 2702
Fragments arranged by size:
3800 2702 711
BsaBI GATnn'nnATC
Cuts at: 7207 7207
Size: 7213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 5020 5402 6037
Size: 53 83 186 936 825 2662 382 635
Cuts at: 6037 275
Size: 1451
Fragments arranged by size:
2662 1451 936 825 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5646 6044 6318 6465 6876 5646
Size: 398 274 147 411 5983
Fragments arranged by size:
5983 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436
Size: 30 165 30 1468 30 45 30 460
Cuts at: 3436 3466 4212 4242 1178
Size: 30 746 30 4149
Fragments arranged by size:
4149 1468 746 460 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2970 3875 1186
Size: 1204 580 905 4524
Fragments arranged by size:
4524 1204 905 580
BseYI C'CCAG_C
Cuts at: 2281 6416 6912 2281
Size: 4135 496 2582
Fragments arranged by size:
4135 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6640
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4663 5424 6452 1540
Size: 275 186 2662 761 1028 2301
Fragments arranged by size:
2662 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3576 5274 5359 6430 2632
Size: 944 1698 85 1071 3415
Fragments arranged by size:
3415 1698 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2497 4724 4817 2497
Size: 2227 93 4893
Fragments arranged by size:
4893 2227 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2963 2793
Size: 170 7043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2159 2632 3576 4532 5274 5359
Size: 399 350 19 473 944 956 742 85
Cuts at: 5359 6430 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 956 944 742 473 399 350
85 19
BspEI T'CCGG_A
Cuts at: 6876 6876
Size: 7213
BspHI T'CATG_A
Cuts at: 3846 5102 6832 3846
Size: 1256 1730 4227
Fragments arranged by size:
4227 1730 1256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4568 844
Size: 3724 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5100 1394
Size: 429 3277 3507
Fragments arranged by size:
3507 3277 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5708 5882 65
Size: 1183 4460 174 1396
Fragments arranged by size:
4460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5857 6044 1700
Size: 110 4047 187 2869
Fragments arranged by size:
4047 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7213
BssSI C'ACGA_G
Cuts at: 2853 4251 5273 6285 2853
Size: 1398 1022 1012 3781
Fragments arranged by size:
3781 1398 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7213
BstBI TT'CG_AA
Cuts at: 6017 6017
Size: 7213
BstEII G'GTnAC_C
Cuts at: 1184 4354 4363 1184
Size: 3170 9 4034
Fragments arranged by size:
4034 3170 9
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 4036 2309
Size: 1727 5486
BstYI r'GATC_y
Cuts at: 1972 2085 2393 3160 4478 4543 4910 5295 5312
Size: 113 308 767 1318 65 367 385 17
Cuts at: 5312 6764 6850 6862 7208 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1318 767 385 367 346 308
113 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 4177 513
Size: 552 1576 1536 3549
Fragments arranged by size:
3549 1576 1536 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6474
Fragments arranged by size:
6474 683 56
BtsI GCAGTG_nn'
Cuts at: 4729 5523 5543 4729
Size: 794 20 6399
Fragments arranged by size:
6399 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7178
DraI TTT'AAA
Cuts at: 3759 4517 3759
Size: 758 6455
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 7213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6220 809
Size: 507 4904 1802
Fragments arranged by size:
4904 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4660 5549 7178
Size: 54 1425 50 275 18 2830 889 1629
Cuts at: 7178 8
Size: 43
Fragments arranged by size:
2830 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4660 1537
Size: 275 2848 4090
Fragments arranged by size:
4090 2848 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2791 3684 4344 5143 1353
Size: 1438 893 660 799 3423
Fragments arranged by size:
3423 1438 893 799 660
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5800 6174 6320 387
Size: 1064 138 4211 374 146 1280
Fragments arranged by size:
4211 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 3574
Size: 7213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2915 3609 4055 4110 4188 4326
Size: 393 526 757 694 446 55 78 138
Cuts at: 4326 4352 4412 5276 5873 6660 6928 1239
Size: 26 60 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 757 694 597 526 446
393 268 138 78 60 55 26
EcoO109I rG'GnC_Cy
Cuts at: 2788 3094 4094 4274 2788
Size: 306 1000 180 5727
Fragments arranged by size:
5727 1000 306 180
EcoRI G'AATT_C
Cuts at: 4133 4522 4900 4133
Size: 389 378 6446
Fragments arranged by size:
6446 389 378
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 7213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2413 2759 3338 4085 2188
Size: 225 346 579 747 5316
Fragments arranged by size:
5316 747 579 346 225
FspI TGC'GCA
Cuts at: 5719 5719
Size: 7213
HaeII r_GCGC'y
Cuts at: 1678 2350 3274 3529 6360 1678
Size: 672 924 255 2831 2531
Fragments arranged by size:
2831 2531 924 672 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946
Size: 32 135 32 505 32 623 32 182
Cuts at: 2946 2978 5854 5886 5928 5960 1373
Size: 32 2876 32 42 32 2626
Fragments arranged by size:
2876 2626 623 505 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2529 4557 4803 669
Size: 988 281 115 476 2028 246 3079
Fragments arranged by size:
3079 2028 988 476 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7213
HpaI GTT'AAC
Cuts at: 2053 4803 2053
Size: 2750 4463
KpnI G_GTAC'C
Cuts at: 2709 4538 2709
Size: 1829 5384
MfeI C'AATT_G
Cuts at: 2988 4812 2988
Size: 1824 5389
MluI A'CGCG_T
Cuts at: 7089 7089
Size: 7213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3889 6327 6511 1305
Size: 2584 2438 184 2007
Fragments arranged by size:
2584 2438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5391
Fragments arranged by size:
5391 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 4442 5171 5530 5689 518
Size: 648 1141 2135 729 359 159 2042
Fragments arranged by size:
2135 2042 1141 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 3398 4629 5306 6454 6699 1606
Size: 1156 636 1231 677 1148 245 2120
Fragments arranged by size:
2120 1231 1156 1148 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2641 4177 513
Size: 552 1576 1536 3549
Fragments arranged by size:
3549 1576 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7213
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7213
NotI GC'GGCC_GC
Cuts at: 4660 4660
Size: 7213
NsiI A_TGCA'T
Cuts at: 3241 3422 3241
Size: 181 7032
NspI r_CATG'y
Cuts at: 1132 1229 4571 6116 1132
Size: 97 3342 1545 2229
Fragments arranged by size:
3342 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7213
PciI A'CATG_T
Cuts at: 6112 6112
Size: 7213
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7213
PmeI GTTT'AAAC
Cuts at: 4517 4517
Size: 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5282 5314 6795 6827 5282
Size: 32 1481 32 5668
Fragments arranged by size:
5668 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 4094 4274 3094
Size: 1000 180 6033
Fragments arranged by size:
6033 1000 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7213
PsiI TTA'TAA
Cuts at: 4025 4783 4025
Size: 758 6455
PstI C_TGCA'G
Cuts at: 830 3294 3562 4565 830
Size: 2464 268 1003 3478
Fragments arranged by size:
3478 2464 1003 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7213
PvuII CAG'CTG
Cuts at: 2762 3398 2762
Size: 636 6577
SacI G_AGCT'C
Cuts at: 3576 3576
Size: 7213
SalI G'TCGA_C
Cuts at: 1936 4555 1936
Size: 2619 4594
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 7213
SbfI CC_TGCA'GG
Cuts at: 4565 4565
Size: 7213
ScaI AGT'ACT
Cuts at: 5461 7173 5461
Size: 1712 5501
SexAI A'CCwGG_T
Cuts at: 3739 3739
Size: 7213
SfcI C'TryA_G
Cuts at: 826 1045 3290 3558 4561 5696 6377 6568 826
Size: 219 2245 268 1003 1135 681 191 1471
Fragments arranged by size:
2245 1471 1135 1003 681 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7213
SmaI CCC'GGG
Cuts at: 1872 4540 1872
Size: 2668 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5317 6218 6480 820
Size: 197 926 3374 901 262 1553
Fragments arranged by size:
3374 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5004 493
Size: 4511 2702
SpeI A'CTAG_T
Cuts at: 152 3638 152
Size: 3486 3727
SphI G_CATG'C
Cuts at: 1132 4571 1132
Size: 3439 3774
SspI AAT'ATT
Cuts at: 5 52 5137 5
Size: 47 5085 2081
Fragments arranged by size:
5085 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7213
StyI C'CwwG_G
Cuts at: 513 1065 2119 2532 2641 2791 4177 4359 4652
Size: 552 1054 413 109 150 1386 182 293
Cuts at: 4652 513
Size: 3074
Fragments arranged by size:
3074 1386 1054 552 413 293 182 150
109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5240 5257 5410 5595 6040 325
Size: 1255 3660 17 153 185 445 1498
Fragments arranged by size:
3660 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5459 7171 96
Size: 274 80 33 51 1443 3482 1712 138
Fragments arranged by size:
3482 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2871 2876 2903 3436 3468 4212 4244 7173
Size: 27 5 27 533 32 744 32 2929
Cuts at: 7173 7205 2844
Size: 32 2852
Fragments arranged by size:
2929 2852 744 533 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7213
XbaI T'CTAG_A
Cuts at: 4481 4549 4481
Size: 68 7145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 7213
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7213
XmaI C'CCGG_G
Cuts at: 1870 4538 1870
Size: 2668 4545
XmnI GAAnn'nnTTC
Cuts at: 2171 5342 2171
Size: 3171 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5021 276
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI