Restriction map for
pFN21AE2768
(Circular) (Six-base) MAPSORT of: pFN21AE2768.seq Check: 578 from: 1 to: 5377
pFN21AE2768 5377 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2102 2102
Size: 5377
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4824 5092 1632
Size: 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 268
AfeI AGC'GCT
Cuts at: 2628 2628
Size: 5377
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 2415 4208 2415
Size: 1793 3584
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5377
AlwNI CAG_nnn'CTG
Cuts at: 2163 2298 2430 2553 2617 3120 4692 2163
Size: 135 132 123 64 503 1572 2848
Fragments arranged by size:
2848 1572 503 135 132 123 64
ApaI G_GGCC'C
Cuts at: 2643 2643
Size: 5377
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2056 2686 2912 3064 5173 1493
Size: 563 630 226 152 2109 1697
Fragments arranged by size:
2109 1697 630 563 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 1870
Size: 73 759 4545
Fragments arranged by size:
4545 759 73
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 4153 618
Size: 325 217 627 911 1455 1842
Fragments arranged by size:
1842 1455 911 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2315 2643 2696 1391
Size: 924 328 53 4072
Fragments arranged by size:
4072 924 328 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2068 928
Size: 971 169 4237
Fragments arranged by size:
4237 971 169
BbvCI CC'TCA_GC
Cuts at: 2451 2451
Size: 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3568 3602 1903
Size: 34 1631 34 3678
Fragments arranged by size:
3678 1631 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BfrBI ATG'CAT
Cuts at: 2217 2217
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3988 136
Size: 107 122 71 631 605 2316 1525
Fragments arranged by size:
2316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2643 3438 4594 1790
Size: 358 495 795 1156 2573
Fragments arranged by size:
2573 1156 795 495 358
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3108 4066
Size: 416 53 83 399 24 1053 638 958
Cuts at: 4066 442
Size: 1753
Fragments arranged by size:
1753 1053 958 638 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2383 4037 1239
Size: 1144 1654 2579
Fragments arranged by size:
2579 1654 1144
Bpu10I CC'TnA_GC
Cuts at: 2309 2451 2630 2309
Size: 142 179 5056
Fragments arranged by size:
5056 179 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3566 4201 275
Size: 53 83 186 936 1651 382 635 1451
Fragments arranged by size:
1651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2415 3810 4208 4482 4629 5040 2415
Size: 1395 398 274 147 411 2752
Fragments arranged by size:
2752 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 2402 2432 2456
Size: 30 165 30 909 30 60 30 24
Cuts at: 2456 2486 1178
Size: 30 4069
Fragments arranged by size:
4069 909 165 60 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4230
BseYI C'CCAG_C
Cuts at: 2043 2610 4580 5076 2043
Size: 567 1970 496 2344
Fragments arranged by size:
2344 1970 567 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2585 1163
Size: 573 849 3955
Fragments arranged by size:
3955 849 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2315 2399 2415 3438 3523 4594 2315
Size: 84 16 1023 85 1071 3098
Fragments arranged by size:
3098 1071 1023 85 84 16
BsmI GAATG_Cn'
Cuts at: 2219 2334 2888 2981 2219
Size: 115 554 93 4615
Fragments arranged by size:
4615 554 115 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2315 2399 2415 2643 2696 3438
Size: 399 358 167 84 16 228 53 742
Cuts at: 3438 3523 4594 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 228 167 85
84 53 16
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2102 2732 844
Size: 1258 630 3489
Fragments arranged by size:
3489 1258 630
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 4021 4208 1700
Size: 110 605 1606 187 2869
Fragments arranged by size:
2869 1606 605 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 2128 1184
Size: 944 4433
BstYI r'GATC_y
Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372
Size: 735 367 385 17 1452 86 12 346
Cuts at: 5372 1972
Size: 1977
Fragments arranged by size:
1977 1452 735 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4825
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 3713 5342
Size: 54 1425 50 275 18 994 889 1629
Cuts at: 5342 8
Size: 43
Fragments arranged by size:
1629 1425 994 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3307 1353
Size: 1954 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2313 2313
Size: 5377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2383 3440 4037 4824 5092 1239
Size: 393 751 1057 597 787 268 1524
Fragments arranged by size:
1524 1057 787 751 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2160 2639 2160
Size: 479 4898
EcoRI G'AATT_C
Cuts at: 2056 2686 3064 2056
Size: 630 378 4369
Fragments arranged by size:
4369 630 378
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 2630 4524 1678
Size: 952 1894 2531
Fragments arranged by size:
2531 1894 952
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4018 4050 4092 4124 1373
Size: 32 135 32 2446 32 42 32 2626
Fragments arranged by size:
2626 2446 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 2502 748
Size: 1754 3623
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 4675 1305
Size: 3186 184 2007
Fragments arranged by size:
3186 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 3335 3694 3853 518
Size: 648 939 1230 359 159 2042
Fragments arranged by size:
2042 1230 939 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2438 2793 3470 4618 4863 1606
Size: 832 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 832 677 355 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4825
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NsiI A_TGCA'T
Cuts at: 2219 2219
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2608 2735 4280 1132
Size: 97 1379 127 1545 2229
Fragments arranged by size:
2229 1545 1379 127 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5377
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2298 1108
Size: 1190 4187
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2639 2639
Size: 5377
PstI C_TGCA'G
Cuts at: 830 2729 830
Size: 1899 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
SacI G_AGCT'C
Cuts at: 2315 2315
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2725 3860 4541 4732 826
Size: 219 1680 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2735 1132
Size: 1603 3774
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5377
StyI C'CwwG_G
Cuts at: 513 1065 2816 513
Size: 552 1751 3074
Fragments arranged by size:
3074 1751 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 5337 5369 2312
Size: 32 2993 32 2320
Fragments arranged by size:
2993 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 2430 1316
Size: 810 304 4263
Fragments arranged by size:
4263 810 304
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 2114 2357 3506 2114
Size: 243 1149 3985
Fragments arranged by size:
3985 1149 243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI