Restriction map for
pFN21AE2767
(Circular) (Six-base) MAPSORT of: pFN21AE2767.seq Check: 946 from: 1 to: 10171
pFN21AE2767 10171 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 3380 3755 4708 5167 5529 2784
Size: 596 375 953 459 362 7426
Fragments arranged by size:
7426 953 596 459 375 362
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7981 278
Size: 53 83 186 936 6445 2468
Fragments arranged by size:
6445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4566 7514 1937
Size: 2629 2948 4594
Fragments arranged by size:
4594 2948 2629
Acc65I G'GTAC_C
Cuts at: 7492 7492
Size: 10171
AclI AA'CG_TT
Cuts at: 1563 8298 8671 1563
Size: 6735 373 3063
Fragments arranged by size:
6735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 4523 4721 8234 9618 9886 1632
Size: 962 1929 198 3513 1384 268 1917
Fragments arranged by size:
3513 1929 1917 1384 962 268 198
AflII C'TTAA_G
Cuts at: 820 1017 3986 820
Size: 197 2969 7005
Fragments arranged by size:
7005 2969 197
AflIII A'CryG_T
Cuts at: 1203 3270 3944 6430 6792 9070 10047 1203
Size: 2067 674 2486 362 2278 977 1327
Fragments arranged by size:
2486 2278 2067 1327 977 674 362
AgeI A'CCGG_T
Cuts at: 9002 9002
Size: 10171
AhdI GACnn_n'nnGTC
Cuts at: 2917 4978 8900 2917
Size: 2061 3922 4188
Fragments arranged by size:
4188 3922 2061
AleI CACnn'nnGTG
Cuts at: 1166 2933 4652 1166
Size: 1767 1719 6685
Fragments arranged by size:
6685 1767 1719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2107 2792 4641 1184
Size: 923 685 1849 6714
Fragments arranged by size:
6714 1849 923 685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7442 7474 7442
Size: 32 10139
AlwNI CAG_nnn'CTG
Cuts at: 2470 4885 4916 6703 7360 7914 9486 2470
Size: 2415 31 1787 657 554 1572 3155
Fragments arranged by size:
3155 2415 1787 1572 657 554 31
ApaI G_GGCC'C
Cuts at: 3127 3247 4086 5275 5811 6252 6315 6627 6811
Size: 120 839 1189 536 441 63 312 184
Cuts at: 6811 7354 3127
Size: 543 5944
Fragments arranged by size:
5944 1189 839 543 536 441 312 184
120 63
ApaLI G'TGCA_C
Cuts at: 2737 3367 4103 6869 8228 9384 2737
Size: 630 736 2766 1359 1156 3524
Fragments arranged by size:
3524 2766 1359 1156 736 630
ApoI r'AATT_y
Cuts at: 1493 7480 7706 7858 9967 1493
Size: 5987 226 152 2109 1697
Fragments arranged by size:
5987 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8725 160
Size: 872 7693 1606
Fragments arranged by size:
7693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10171
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 3138 3871 4515 4791 6308 6358
Size: 73 532 663 733 644 276 1517 50
Cuts at: 6358 7094 7496 1870
Size: 736 402 4545
Fragments arranged by size:
4545 1517 736 733 663 644 532 402
276 73 50
BamHI G'GATC_C
Cuts at: 3099 7501 7868 3099
Size: 4402 367 5402
Fragments arranged by size:
5402 4402 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2757 2822 2865 2885 2923
Size: 325 217 627 970 65 43 20 38
Cuts at: 2923 3920 4073 4450 4672 4840 5096 5387 5536
Size: 997 153 377 222 168 256 291 149
Cuts at: 5536 6238 6413 7492 8947 618
Size: 702 175 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 997 970 702 627 377
325 291 256 222 217 175 168 153
149 65 43 38 20
BanII G_rGCy'C
Cuts at: 1391 2390 2583 3127 3247 4086 4133 5275 5553
Size: 999 193 544 120 839 47 1142 278
Cuts at: 5553 5608 5811 6135 6192 6252 6315 6428 6627
Size: 55 203 324 57 60 63 113 199
Cuts at: 6627 6811 6884 6965 7257 7323 7354 7490 1391
Size: 184 73 81 292 66 31 136 4072
Fragments arranged by size:
4072 1142 999 839 544 324 292 278
203 199 193 184 136 120 113 81
73 66 63 60 57 55 47 31
BbeI G_GCGC'C
Cuts at: 2761 2826 4077 4676 5391 6242 2761
Size: 65 1251 599 715 851 6690
Fragments arranged by size:
6690 1251 851 715 599 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2776 3185 4287 6774 928
Size: 971 877 409 1102 2487 4325
Fragments arranged by size:
4325 2487 1102 971 877 409
BbvCI CC'TCA_GC
Cuts at: 3734 7121 3734
Size: 3387 6784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3654 3688 6502 6536 8362 8396 1903
Size: 34 1717 34 2814 34 1826 34 3678
Fragments arranged by size:
3678 2814 1826 1717 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8063 9273 8063
Size: 1210 8961
BclI T'GATC_A
Cuts at: 1548 2055 1548
Size: 507 9664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3141 4128 4232
Size: 107 122 71 631 605 1469 987 104
Cuts at: 4232 4459 4691 5603 5968 6247 6822 7000 8782
Size: 227 232 912 365 279 575 178 1782
Cuts at: 8782 136
Size: 1525
Fragments arranged by size:
1782 1525 1469 987 912 631 605 575
365 279 232 227 178 122 107 104
71
BglII A'GATC_T
Cuts at: 4575 10166 4575
Size: 5591 4580
BlpI GC'TnA_GC
Cuts at: 5252 5717 5727 6449 6980 7588 5252
Size: 465 10 722 531 608 7835
Fragments arranged by size:
7835 722 608 531 465 10
Bme1580I G_kGCm'C
Cuts at: 1790 2741 2888 2928 3127 3247 3371 3570 3923
Size: 951 147 40 199 120 124 199 353
Cuts at: 3923 4086 4107 4455 4845 4908 5275 5541 5664
Size: 163 21 348 390 63 367 266 123
Cuts at: 5664 5811 6252 6315 6438 6588 6627 6811 6873
Size: 147 441 63 123 150 39 184 62
Cuts at: 6873 6934 7137 7354 8232 9388 1790
Size: 61 203 217 878 1156 2573
Fragments arranged by size:
2573 1156 951 878 441 390 367 353
348 266 217 203 199 199 184 163
150 147 147 124 123 123 120 63
63 62 61 40 39 21
BmgBI CAC'GTC
Cuts at: 1318 3782 4369 4903 4975 5245 1318
Size: 2464 587 534 72 270 6244
Fragments arranged by size:
6244 2464 587 534 270 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2890 3047
Size: 416 53 83 399 24 682 791 157
Cuts at: 3047 3198 6921 7902 8860 442
Size: 151 3723 981 958 1753
Fragments arranged by size:
3723 1753 981 958 791 682 416 399
157 151 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 3178 2695
Size: 483 9688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2402 3101 3478 4580 5210 7382 8831
Size: 1145 18 699 377 1102 630 2172 1449
Cuts at: 8831 1239
Size: 2579
Fragments arranged by size:
2579 2172 1449 1145 1102 699 630 377
18
Bpu10I CC'TnA_GC
Cuts at: 2483 3734 5409 5607 5928 6422 6959 7121 7436
Size: 1251 1675 198 321 494 537 162 315
Cuts at: 7436 2483
Size: 5218
Fragments arranged by size:
5218 1675 1251 537 494 321 315 198
162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 3269 3320 4340 4730 8296 9161 9459 2435
Size: 834 51 1020 390 3566 865 298 3147
Fragments arranged by size:
3566 3147 1020 865 834 390 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2264 5080 5732 8834 882
Size: 617 765 2816 652 3102 2219
Fragments arranged by size:
3102 2816 2219 765 652 617
BsaAI yAC'GTr
Cuts at: 493 1204 3256 3271 3945 6171 6433 6793 7962
Size: 711 2052 15 674 2226 262 360 1169
Cuts at: 7962 493
Size: 2702
Fragments arranged by size:
2702 2226 2052 1169 711 674 360 262
15
BsaBI GATnn'nnATC
Cuts at: 10165 10165
Size: 10171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2758 2823 4074 4673
Size: 53 83 186 936 1225 65 1251 599
Cuts at: 4673 4788 5388 5677 6012 6231 6239 7978 8360
Size: 115 600 289 335 219 8 1739 382
Cuts at: 8360 8995 275
Size: 635 1451
Fragments arranged by size:
1739 1451 1251 1225 936 635 600 599
382 335 289 219 186 115 83 65
53 8
BsaWI w'CCGG_w
Cuts at: 2652 4580 5331 5844 8604 9002 9276 9423 9834
Size: 1928 751 513 2760 398 274 147 411
Cuts at: 9834 2652
Size: 2989
Fragments arranged by size:
2989 2760 1928 751 513 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 3411 3441 3545
Size: 30 165 30 1641 30 337 30 104
Cuts at: 3545 3575 3878 3908 4578 4608 6644 6674 1178
Size: 30 303 30 670 30 2036 30 4675
Fragments arranged by size:
4675 2036 1641 670 337 303 165 104
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2360 3022 3098 4013 4148 4151 4154
Size: 1096 78 662 76 915 135 3 3
Cuts at: 4154 4562 5689 6161 6214 6544 7068 1186
Size: 408 1127 472 53 330 524 4289
Fragments arranged by size:
4289 1127 1096 915 662 524 472 408
330 135 78 76 53 3 3
BseYI C'CCAG_C
Cuts at: 2021 4527 5417 5663 5712 5721 5877 5919 5958
Size: 2506 890 246 49 9 156 42 39
Cuts at: 5958 6092 6139 6825 6984 7237 7353 9374 9870
Size: 134 47 686 159 253 116 2021 496
Cuts at: 9870 2021
Size: 2322
Fragments arranged by size:
2506 2322 2021 890 686 496 253 246
159 156 134 116 49 47 42 39
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2140 3254 3466 3923 4027 6362
Size: 573 365 39 1114 212 457 104 2335
Cuts at: 6362 7172 1163
Size: 810 4162
Fragments arranged by size:
4162 2335 1114 810 573 457 365 212
104 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4930 6671 7621 8382 9410
Size: 275 186 1546 1383 1741 950 761 1028
Cuts at: 9410 1540
Size: 2301
Fragments arranged by size:
2301 1741 1546 1383 1028 950 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2741 3255 3371 3542 4107 5553 6192 6327 6873
Size: 514 116 171 565 1446 639 135 546
Cuts at: 6873 6923 8232 8317 9388 2741
Size: 50 1309 85 1071 3524
Fragments arranged by size:
3524 1446 1309 1071 639 565 546 514
171 135 116 85 50
BsiWI C'GTAC_G
Cuts at: 4834 4834
Size: 10171
BsmI GAATG_Cn'
Cuts at: 3959 7070 7682 7775 3959
Size: 3111 612 93 6355
Fragments arranged by size:
6355 3111 612 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4965 5701 6103 6556 4965
Size: 736 402 453 8580
Fragments arranged by size:
8580 736 453 402
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 2583 2741 2888 2928 3127 3247
Size: 399 600 193 158 147 40 199 120
Cuts at: 3247 3255 3371 3542 3570 3923 4086 4107 4133
Size: 8 116 171 28 353 163 21 26
Cuts at: 4133 4455 4845 4908 5275 5541 5553 5608 5664
Size: 322 390 63 367 266 12 55 56
Cuts at: 5664 5811 6135 6192 6252 6315 6327 6428 6438
Size: 147 324 57 60 63 12 101 10
Cuts at: 6438 6588 6627 6811 6873 6884 6923 6934 6965
Size: 150 39 184 62 11 39 11 31
Cuts at: 6965 7137 7257 7323 7354 7490 8232 8317 9388
Size: 172 120 66 31 136 742 85 1071
Cuts at: 9388 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 600 399 390 367 353
324 322 266 199 193 184 172 171
163 158 150 147 147 136 120 120
116 101 85 66 63 63 62 60
57 56 55 40 39 39 31 31
28 26 21 12 12 11 11 10
8
BspEI T'CCGG_A
Cuts at: 2652 9834 2652
Size: 7182 2989
BspHI T'CATG_A
Cuts at: 2844 8060 9790 2844
Size: 5216 1730 3225
Fragments arranged by size:
5216 3225 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2784 3380 3479 3755 4506 4708 5167 5529
Size: 1940 596 99 276 751 202 459 362
Cuts at: 5529 6165 7526 844
Size: 636 1361 3489
Fragments arranged by size:
3489 1940 1361 751 636 596 459 362
276 202 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 2674 3105 4174 4680 5932 6021
Size: 429 527 324 431 1069 506 1252 89
Cuts at: 6021 6495 6667 6991 8058 1394
Size: 474 172 324 1067 3507
Fragments arranged by size:
3507 1252 1069 1067 527 506 474 431
429 324 324 172 89
BsrDI GCAATG_nn'
Cuts at: 65 1248 3664 3745 3766 8666 8840 65
Size: 1183 2416 81 21 4900 174 1396
Fragments arranged by size:
4900 2416 1396 1183 174 81 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 3485 3923 4690 5731 5915 7101
Size: 110 368 1307 438 767 1041 184 1186
Cuts at: 7101 8815 9002 1700
Size: 1714 187 2869
Fragments arranged by size:
2869 1714 1307 1186 1041 767 438 368
187 184 110
BsrGI T'GTAC_A
Cuts at: 96 2400 4585 6048 96
Size: 2304 2185 1463 4219
Fragments arranged by size:
4219 2304 2185 1463
BssHII G'CGCG_C
Cuts at: 1925 5903 1925
Size: 3978 6193
BssSI C'ACGA_G
Cuts at: 2740 5341 8231 9243 2740
Size: 2601 2890 1012 3668
Fragments arranged by size:
3668 2890 2601 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4227 4916 7270 794
Size: 3433 689 2354 3695
Fragments arranged by size:
3695 3433 2354 689
BstBI TT'CG_AA
Cuts at: 8975 8975
Size: 10171
BstEII G'GTnAC_C
Cuts at: 1184 3157 4384 5707 7030 1184
Size: 1973 1227 1323 1323 4325
Fragments arranged by size:
4325 1973 1323 1323 1227
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 3070 4447 4975 6604 2833
Size: 237 1377 528 1629 6400
Fragments arranged by size:
6400 1629 1377 528 237
BstYI r'GATC_y
Cuts at: 1972 2676 3099 4575 5430 7501 7868 8253 8270
Size: 704 423 1476 855 2071 367 385 17
Cuts at: 8270 9722 9808 9820 10166 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2071 1977 1476 1452 855 704 423 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2700 4860 2700
Size: 2160 8011
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2211 2391 2880 3547 3678
Size: 552 939 23 184 180 489 667 131
Cuts at: 3678 4059 5011 5127 5703 6244 6656 6995 513
Size: 381 952 116 576 541 412 339 3689
Fragments arranged by size:
3689 952 939 667 576 552 541 489
412 381 339 184 180 131 116 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6069 487
Size: 683 56 4843 4589
Fragments arranged by size:
4843 4589 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 3911 4773 4914 6304 6518 6951 7687 8481
Size: 103 862 141 1390 214 433 736 794
Cuts at: 8481 8501 3808
Size: 20 5478
Fragments arranged by size:
5478 1390 862 794 736 433 214 141
103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10136
DraI TTT'AAA
Cuts at: 7475 7475
Size: 10171
DraIII CAC_nnn'GTG
Cuts at: 2124 3574 4267 6331 6445 2124
Size: 1450 693 2064 114 5850
Fragments arranged by size:
5850 2064 1450 693 114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9178 809
Size: 507 7862 1802
Fragments arranged by size:
7862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3463 3544
Size: 54 1425 50 275 18 378 1255 81
Cuts at: 3544 3784 3799 4683 4927 5952 6207 6337 6668
Size: 240 15 884 244 1025 255 130 331
Cuts at: 6668 6992 7618 8507 10136 8
Size: 324 626 889 1629 43
Fragments arranged by size:
1629 1425 1255 1025 889 884 626 378
331 324 275 255 244 240 130 81
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3544 4927 6668 7618 1537
Size: 275 1732 1383 1741 950 4090
Fragments arranged by size:
4090 1741 1732 1383 950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 7262 8101 1353
Size: 752 5157 839 3423
Fragments arranged by size:
5157 3423 839 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 5222 6895 8758 9132 9278
Size: 1064 138 624 3009 1673 1863 374 146
Cuts at: 9278 387
Size: 1280
Fragments arranged by size:
3009 1863 1673 1280 1064 624 374 146
138
EcoICRI GAG'CTC
Cuts at: 5551 6190 5551
Size: 639 9532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2402 2594 3101 3478 4523 4580
Size: 393 752 18 192 507 377 1045 57
Cuts at: 4580 4721 5210 7382 8234 8831 9618 9886 1239
Size: 141 489 2172 852 597 787 268 1524
Fragments arranged by size:
2172 1524 1045 852 787 752 597 507
489 393 377 268 192 141 57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3518 3881 4559 3518
Size: 363 678 9130
Fragments arranged by size:
9130 678 363
EcoO109I rG'GnC_Cy
Cuts at: 2076 2101 2479 3124 3192 3244 3886 4731 5271
Size: 25 378 645 68 52 642 845 540
Cuts at: 5271 5314 5618 5924 6249 6312 6623 7223 7350
Size: 43 304 306 325 63 311 600 127
Cuts at: 7350 2076
Size: 4897
Fragments arranged by size:
4897 845 645 642 600 540 378 325
311 306 304 127 68 63 52 43
25
EcoRI G'AATT_C
Cuts at: 7480 7858 7480
Size: 378 9793
EcoRV GAT'ATC
Cuts at: 7015 7015
Size: 10171
FspI TGC'GCA
Cuts at: 4343 8677 4343
Size: 4334 5837
HaeII r_GCGC'y
Cuts at: 1678 2185 2761 2826 4077 4676 5391 6223 6242
Size: 507 576 65 1251 599 715 832 19
Cuts at: 6242 7314 9318 1678
Size: 1072 2004 2531
Fragments arranged by size:
2531 2004 1251 1072 832 715 599 576
507 65 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2661 2693 2903 2935 3044
Size: 32 135 32 1089 32 210 32 109
Cuts at: 3044 3076 3380 3412 3878 3910 4964 4996 5492
Size: 32 304 32 466 32 1054 32 496
Cuts at: 5492 5524 6467 6499 8812 8844 8886 8918 1373
Size: 32 943 32 2313 32 42 32 2626
Fragments arranged by size:
2626 2313 1089 1054 943 496 466 304
210 135 109 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5206 5785 5830 7515 7761 669
Size: 988 281 3268 579 45 1685 246 3079
Fragments arranged by size:
3268 3079 1685 988 579 281 246 45
HindIII A'AGCT_T
Cuts at: 748 3008 4703 748
Size: 2260 1695 6216
Fragments arranged by size:
6216 2260 1695
HpaI GTT'AAC
Cuts at: 7761 7761
Size: 10171
KasI G'GCGC_C
Cuts at: 2757 2822 4073 4672 5387 6238 2757
Size: 65 1251 599 715 851 6690
Fragments arranged by size:
6690 1251 851 715 599 65
KpnI G_GTAC'C
Cuts at: 7496 7496
Size: 10171
MfeI C'AATT_G
Cuts at: 7770 7770
Size: 10171
MluI A'CGCG_T
Cuts at: 10047 10047
Size: 10171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2445 3087 3132 3351 3686 6252 9285 9469
Size: 1140 642 45 219 335 2566 3033 184
Cuts at: 9469 1305
Size: 2007
Fragments arranged by size:
3033 2566 2007 1140 642 335 219 184
45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3465 3786 5954 10
Size: 54 1425 343 1633 321 2168 4227
Fragments arranged by size:
4227 2168 1633 1425 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2933 3258 4334 4652 4901 5126
Size: 648 1242 525 325 1076 318 249 225
Cuts at: 5126 7445 8129 8488 8647 518
Size: 2319 684 359 159 2042
Fragments arranged by size:
2319 2042 1242 1076 684 648 525 359
325 318 249 225 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2213 2224 2732 3238 3280 3804 4347
Size: 485 122 11 508 506 42 524 543
Cuts at: 4347 4377 4402 4531 4882 5013 5417 5716 6216
Size: 30 25 129 351 131 404 299 500
Cuts at: 6216 6658 6673 6700 6943 6979 7237 7270 7428
Size: 442 15 27 243 36 258 33 158
Cuts at: 7428 7587 8264 9412 9657 1606
Size: 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 543 524 508 506 500
485 442 404 351 299 258 245 243
159 158 131 129 122 42 36 33
30 27 25 15 11
NaeI GCC'GGC
Cuts at: 4692 5733 5917 7103 4692
Size: 1041 184 1186 7760
Fragments arranged by size:
7760 1186 1041 184
NarI GG'CG_CC
Cuts at: 2758 2823 4074 4673 5388 6239 2758
Size: 65 1251 599 715 851 6690
Fragments arranged by size:
6690 1251 851 715 599 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 2027 3678 4059 513
Size: 552 939 23 1651 381 6625
Fragments arranged by size:
6625 1651 939 552 381 23
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10171
NgoMIV G'CCGG_C
Cuts at: 4690 5731 5915 7101 4690
Size: 1041 184 1186 7760
Fragments arranged by size:
7760 1186 1041 184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10171
NotI GC'GGCC_GC
Cuts at: 6668 7618 6668
Size: 950 9221
NspI r_CATG'y
Cuts at: 1132 1229 4294 4399 7391 7529 9074 1132
Size: 97 3065 105 2992 138 1545 2229
Fragments arranged by size:
3065 2992 2229 1545 138 105 97
PasI CC'CwG_GG
Cuts at: 1348 4079 6525 6619 7345 1348
Size: 2731 2446 94 726 4174
Fragments arranged by size:
4174 2731 2446 726 94
PciI A'CATG_T
Cuts at: 9070 9070
Size: 10171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 2779 4543 7360 1108
Size: 920 751 1764 2817 3919
Fragments arranged by size:
3919 2817 1764 920 751
PfoI T'CCnGG_A
Cuts at: 4023 4809 5052 5187 5423 4023
Size: 786 243 135 236 8771
Fragments arranged by size:
8771 786 243 236 135
PmeI GTTT'AAAC
Cuts at: 7475 7475
Size: 10171
PmlI CAC'GTG
Cuts at: 3256 3271 3945 6171 6433 3256
Size: 15 674 2226 262 6994
Fragments arranged by size:
6994 2226 674 262 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5129 5161 8240 8272 9753 9785 3590
Size: 32 1507 32 3079 32 1481 32 3976
Fragments arranged by size:
3976 3079 1507 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2479 3192 3886 5314 5618 7223 2076
Size: 403 713 694 1428 304 1605 5024
Fragments arranged by size:
5024 1605 1428 713 694 403 304
PshAI GACnn'nnGTC
Cuts at: 1613 3155 1613
Size: 1542 8629
PsiI TTA'TAA
Cuts at: 7741 7741
Size: 10171
PspOMI G'GGCC_C
Cuts at: 3123 3243 4082 5271 5807 6248 6311 6623 6807
Size: 120 839 1189 536 441 63 312 184
Cuts at: 6807 7350 3123
Size: 543 5944
Fragments arranged by size:
5944 1189 839 543 536 441 312 184
120 63
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4550 4582 7121 7153 4550
Size: 32 2539 32 7568
Fragments arranged by size:
7568 2539 32 32
PstI C_TGCA'G
Cuts at: 830 2667 2727 3051 3410 4780 5178 6678 6978
Size: 1837 60 324 359 1370 398 1500 300
Cuts at: 6978 7523 830
Size: 545 3478
Fragments arranged by size:
3478 1837 1500 1370 545 398 359 324
300 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10171
PvuII CAG'CTG
Cuts at: 2732 3238 3280 4531 4882 5716 6700 6979 7237
Size: 506 42 1251 351 834 984 279 258
Cuts at: 7237 2732
Size: 5666
Fragments arranged by size:
5666 1251 984 834 506 351 279 258
42
SacI G_AGCT'C
Cuts at: 5553 6192 5553
Size: 639 9532
SacII CC_GC'GG
Cuts at: 2214 5014 6659 2214
Size: 2800 1645 5726
Fragments arranged by size:
5726 2800 1645
SalI G'TCGA_C
Cuts at: 1936 7513 1936
Size: 5577 4594
SanDI GG'GwC_CC
Cuts at: 5314 5618 5314
Size: 304 9867
SapI GCTCTTCn'nnn_
Cuts at: 2105 7262 2105
Size: 5157 5014
SbfI CC_TGCA'GG
Cuts at: 7523 7523
Size: 10171
ScaI AGT'ACT
Cuts at: 4208 5486 8419 10131 4208
Size: 1278 2933 1712 4248
Fragments arranged by size:
4248 2933 1712 1278
SexAI A'CCwGG_T
Cuts at: 3228 5019 6327 7026 3228
Size: 1791 1308 699 6373
Fragments arranged by size:
6373 1791 1308 699
SfcI C'TryA_G
Cuts at: 826 1045 2663 2723 2992 3047 3406 4048 4567
Size: 219 1618 60 269 55 359 642 519
Cuts at: 4567 4776 5174 5623 6674 6682 6974 7519 8654
Size: 209 398 449 1051 8 292 545 1135
Cuts at: 8654 9335 9526 826
Size: 681 191 1471
Fragments arranged by size:
1618 1471 1135 1051 681 642 545 519
449 398 359 292 269 219 209 191
60 55 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 4691 7000 4691
Size: 2309 7862
SfoI GGC'GCC
Cuts at: 2759 2824 4075 4674 5389 6240 2759
Size: 65 1251 599 715 851 6690
Fragments arranged by size:
6690 1251 851 715 599 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10171
SgrAI Cr'CCGG_yG
Cuts at: 1700 3923 1700
Size: 2223 7948
SmaI CCC'GGG
Cuts at: 1872 3873 4793 6310 6360 7498 1872
Size: 2001 920 1517 50 1138 4545
Fragments arranged by size:
4545 2001 1517 1138 920 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 3248 3335 3986 4355 4745
Size: 197 926 507 798 87 651 369 390
Cuts at: 4745 8275 9176 9438 820
Size: 3530 901 262 1553
Fragments arranged by size:
3530 1553 926 901 798 651 507 390
369 262 197 87
SnaBI TAC'GTA
Cuts at: 493 7962 493
Size: 7469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10171
SphI G_CATG'C
Cuts at: 1132 7529 1132
Size: 6397 3774
SspI AAT'ATT
Cuts at: 5 52 8095 5
Size: 47 8043 2081
Fragments arranged by size:
8043 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 2563 3089 3144 4226 5162 1517
Size: 802 244 526 55 1082 936 6526
Fragments arranged by size:
6526 1082 936 802 526 244 55
StyI C'CwwG_G
Cuts at: 513 1065 2004 2027 2079 2314 3678 4059 7048
Size: 552 939 23 52 235 1364 381 2989
Cuts at: 7048 7610 513
Size: 562 3074
Fragments arranged by size:
3074 2989 1364 939 562 552 381 235
52 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 2786 2909 3678 4993 5663 5972
Size: 1255 627 579 123 769 1315 670 309
Cuts at: 5972 6431 7316 7457 8198 8215 8368 8553 8998
Size: 459 885 141 741 17 153 185 445
Cuts at: 8998 325
Size: 1498
Fragments arranged by size:
1498 1315 1255 885 769 741 670 627
579 459 445 309 185 153 141 123
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2898 4206
Size: 274 80 33 51 1443 423 498 1308
Cuts at: 4206 4585 5484 6048 7281 8417 10129 96
Size: 379 899 564 1233 1136 1712 138
Fragments arranged by size:
1712 1443 1308 1233 1136 899 564 498
423 379 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3411 3426 3443 3458 3521 3545 3553 3577 4195
Size: 15 17 15 63 24 8 24 618
Cuts at: 4195 4227 5482 5514 6644 6676 10131 10163 3411
Size: 32 1255 32 1130 32 3455 32 3419
Fragments arranged by size:
3455 3419 1255 1130 618 63 32 32
32 32 24 24 17 15 15 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 3190 4370 1316
Size: 1218 656 1180 7117
Fragments arranged by size:
7117 1218 1180 656
XbaI T'CTAG_A
Cuts at: 7507 7507
Size: 10171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 3794 5947 2041
Size: 1753 2153 6265
Fragments arranged by size:
6265 2153 1753
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10171
XmaI C'CCGG_G
Cuts at: 1870 3871 4791 6308 6358 7496 1870
Size: 2001 920 1517 50 1138 4545
Fragments arranged by size:
4545 2001 1517 1138 920 50
XmnI GAAnn'nnTTC
Cuts at: 8300 8300
Size: 10171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7979 276
Size: 53 83 186 936 6445 2468
Fragments arranged by size:
6445 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BstZ17I FalI FseI
FspAI NruI NsiI PacI PspXI RsrII SrfI SwaI