Restriction map for
pFN21AE2765
(Circular) (Six-base) MAPSORT of: pFN21AE2765.seq Check: 6068 from: 1 to: 5725
pFN21AE2765 5725 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2192
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 3788 5172 5440 1632
Size: 542 1614 1384 268 1917
Fragments arranged by size:
1917 1614 1384 542 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 2965 4454 2965
Size: 1489 4236
AleI CACnn'nnGTG
Cuts at: 1166 2099 1166
Size: 933 4792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 5693
AlwNI CAG_nnn'CTG
Cuts at: 2257 3468 5040 2257
Size: 1211 1572 2942
Fragments arranged by size:
2942 1572 1211
ApaLI G'TGCA_C
Cuts at: 2948 3782 4938 2948
Size: 834 1156 3735
Fragments arranged by size:
3735 1156 834
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2446 3050 1870
Size: 73 503 604 4545
Fragments arranged by size:
4545 604 503 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 2518 2623 2675 2895
Size: 325 217 627 587 144 105 52 220
Cuts at: 2895 3046 4501 618
Size: 151 1455 1842
Fragments arranged by size:
1842 1455 627 587 325 220 217 151
144 105 52
BanII G_rGCy'C
Cuts at: 1391 2142 3044 1391
Size: 751 902 4072
Fragments arranged by size:
4072 902 751
BbeI G_GCGC'C
Cuts at: 2522 2522
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 928
Size: 971 558 4196
Fragments arranged by size:
4196 971 558
BbvCI CC'TCA_GC
Cuts at: 2309 2309
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 2163 1548
Size: 615 5110
BfrBI ATG'CAT
Cuts at: 2839 2839
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2987 4336 136
Size: 107 122 71 631 605 1315 1349 1525
Fragments arranged by size:
1525 1349 1315 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2628 2678 2793 2952 3786 4942 1790
Size: 838 50 115 159 834 1156 2573
Fragments arranged by size:
2573 1156 838 834 159 115 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2914 3456 4414
Size: 416 53 83 399 24 1497 542 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1497 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2300 2420 4385 1239
Size: 917 144 120 1965 2579
Fragments arranged by size:
2579 1965 917 144 120
Bpu10I CC'TnA_GC
Cuts at: 2309 2309
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 2738 2803 5719 2738
Size: 65 2916 2744
Fragments arranged by size:
2916 2744 65
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2519 3532 3914 4549
Size: 53 83 186 936 986 1013 382 635
Cuts at: 4549 275
Size: 1451
Fragments arranged by size:
1451 1013 986 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3006 4158 4556 4830 4977 5388 3006
Size: 1152 398 274 147 411 3343
Fragments arranged by size:
3343 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 2592 2622 1178
Size: 30 165 30 990 30 169 30 4281
Fragments arranged by size:
4281 990 169 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5725
BseYI C'CCAG_C
Cuts at: 2596 2749 4928 5424 2596
Size: 153 2179 496 2897
Fragments arranged by size:
2897 2179 496 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 1163
Size: 573 434 4718
Fragments arranged by size:
4718 573 434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2055 2142 2952 3786 3871 4942 2040
Size: 15 87 810 834 85 1071 2823
Fragments arranged by size:
2823 1071 834 810 87 85 15
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2055 2142 2628 2678 2793 2952
Size: 399 250 15 87 486 50 115 159
Cuts at: 2952 3044 3786 3871 4942 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 399 250 159 115
92 87 85 50 15
BspEI T'CCGG_A
Cuts at: 3006 5388 3006
Size: 2382 3343
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 3080 844
Size: 1348 888 3489
Fragments arranged by size:
3489 1348 888
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 2810 4369 4556 1700
Size: 110 611 389 1559 187 2869
Fragments arranged by size:
2869 1559 611 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2048 3785 4797 2048
Size: 1737 1012 2976
Fragments arranged by size:
2976 1737 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 2257 794
Size: 1399 64 4262
Fragments arranged by size:
4262 1399 64
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 2008 1184
Size: 824 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2644 2128
Size: 516 5209
BstYI r'GATC_y
Cuts at: 1972 2733 3009 3055 3422 3807 3824 5276 5362
Size: 761 276 46 367 385 17 1452 86
Cuts at: 5362 5374 5720 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 761 385 367 346 276 86
46 17 12
Bsu36I CC'TnA_GG
Cuts at: 2456 2504 2456
Size: 48 5677
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 2523 2670 2922 513
Size: 552 939 93 426 147 252 3316
Fragments arranged by size:
3316 939 552 426 252 147 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2862 487
Size: 683 56 1636 3350
Fragments arranged by size:
3350 1636 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 3241 4035 4055 2191
Size: 1050 794 20 3861
Fragments arranged by size:
3861 1050 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2979 3172
Size: 54 1425 50 275 18 273 876 193
Cuts at: 3172 4061 5690 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 876 275 273 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2060 2150 2399 3655 1353
Size: 662 45 90 249 1256 3423
Fragments arranged by size:
3423 1256 662 249 90 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2140
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2174 2300 2420 3788 4385 5172
Size: 393 524 18 126 120 1368 597 787
Cuts at: 5172 5440 1239
Size: 268 1524
Fragments arranged by size:
1524 1368 787 597 524 393 268 126
120 18
EcoO109I rG'GnC_Cy
Cuts at: 2025 2025
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2428 2522 2664 4872 1678
Size: 750 94 142 2208 2531
Fragments arranged by size:
2531 2208 750 142 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2473 2505 2592
Size: 32 135 32 821 32 48 32 87
Cuts at: 2592 2624 2951 2983 4366 4398 4440 4472 1373
Size: 32 327 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 821 327 135 87 48 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2518 2518
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 10
Size: 54 1425 343 273 3630
Fragments arranged by size:
3630 1425 343 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 3683 4042 4201 518
Size: 648 933 1584 359 159 2042
Fragments arranged by size:
2042 1584 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2320 2924 3141 3818 4966 5211 1606
Size: 651 63 604 217 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 604 245 217 63
NaeI GCC'GGC
Cuts at: 2423 2423
Size: 5725
NarI GG'CG_CC
Cuts at: 2519 2519
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 2004 2097 513
Size: 552 939 93 4141
Fragments arranged by size:
4141 939 552 93
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NgoMIV G'CCGG_C
Cuts at: 2421 2421
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NsiI A_TGCA'T
Cuts at: 2841 2841
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2843 3083 4628 1132
Size: 97 1614 240 1545 2229
Fragments arranged by size:
2229 1614 1545 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2098 2112 2569 1108
Size: 990 14 457 4264
Fragments arranged by size:
4264 990 457 14
PfoI T'CCnGG_A
Cuts at: 2133 2589 2133
Size: 456 5269
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2025 2025
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
SacI G_AGCT'C
Cuts at: 2142 2142
Size: 5725
SacII CC_GC'GG
Cuts at: 2925 2925
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5725
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SexAI A'CCwGG_T
Cuts at: 2111 2111
Size: 5725
SfcI C'TryA_G
Cuts at: 826 1045 3073 4208 4889 5080 826
Size: 219 2028 1135 681 191 1471
Fragments arranged by size:
2028 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2987 2987
Size: 5725
SfoI GGC'GCC
Cuts at: 2520 2520
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 4730 4992 820
Size: 197 926 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 2176 1517
Size: 659 5066
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2985 3164 513
Size: 552 939 93 888 179 3074
Fragments arranged by size:
3074 939 888 552 179 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2576 2641 2763 2812 3752 3769
Size: 1255 624 372 65 122 49 940 17
Cuts at: 3769 3922 4107 4552 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 940 624 445 372 185 153
122 65 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 1316
Size: 1182 4543
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2566 2566
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SrfI
SwaI