Restriction map for
pFN21AE2760
(Circular) (Six-base) MAPSORT of: pFN21AE2760.seq Check: 7468 from: 1 to: 5458
pFN21AE2760 5458 bp
With 182 enzymes: SgfI *
August 1, 2019 17:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2725 3521 4905 5173 1632
Size: 1093 796 1384 268 1917
Fragments arranged by size:
1917 1384 1093 796 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2227 2258 1184
Size: 1043 31 4384
Fragments arranged by size:
4384 1043 31
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2321 2459 2522 2767 2993 3145 5254 1493
Size: 828 138 63 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 245 226 152 138 63
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2779 4234 618
Size: 325 217 627 992 1455 1842
Fragments arranged by size:
1842 1455 992 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2434 2698 2777 1391
Size: 1043 264 79 4072
Fragments arranged by size:
4072 1043 264 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2290 3350 4560 2290
Size: 1060 1210 3188
Fragments arranged by size:
3188 1210 1060
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BfrBI ATG'CAT
Cuts at: 2540 2540
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2663 5453 2663
Size: 2790 2668
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 3519 4675 1790
Size: 1729 1156 2573
Fragments arranged by size:
2573 1729 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3189 4147
Size: 416 53 83 399 24 975 797 958
Cuts at: 4147 442
Size: 1753
Fragments arranged by size:
1753 975 958 797 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2712 4118 1239
Size: 1473 1406 2579
Fragments arranged by size:
2579 1473 1406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3265 3647 4282 275
Size: 53 83 186 936 1732 382 635 1451
Fragments arranged by size:
1732 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2421 2451 1178
Size: 30 165 30 621 30 367 30 4185
Fragments arranged by size:
4185 621 367 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5458
BseYI C'CCAG_C
Cuts at: 2472 4661 5157 2472
Size: 2189 496 2773
Fragments arranged by size:
2773 2189 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2531 1163
Size: 573 795 4090
Fragments arranged by size:
4090 795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2604 2908 3669 4697 1540
Size: 275 186 603 304 761 1028 2301
Fragments arranged by size:
2301 1028 761 603 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 3519 3604 4675 2337
Size: 1182 85 1071 3120
Fragments arranged by size:
3120 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 2540 2969 3062 2540
Size: 429 93 4936
Fragments arranged by size:
4936 429 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2434 2698 2777 3519 3604 4675
Size: 399 547 97 264 79 742 85 1071
Cuts at: 4675 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 547 399 264 97 85
79
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2813 844
Size: 1969 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2139 3345 1394
Size: 429 212 104 1206 3507
Fragments arranged by size:
3507 1206 429 212 104
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 96
Size: 2391 3067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2509 794
Size: 1715 3743
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2663 2788 3155 3540 3557 5009 5095 5107
Size: 691 125 367 385 17 1452 86 12
Cuts at: 5107 5453 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 691 385 367 346 125 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4906
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2162 4465 809
Size: 507 846 2303 1802
Fragments arranged by size:
2303 1802 846 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2601 2905
Size: 54 1425 50 275 18 486 285 304
Cuts at: 2905 3794 5423 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 486 304 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2601 2905 1537
Size: 275 789 304 4090
Fragments arranged by size:
4090 789 304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3388 1353
Size: 2035 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2350 4045 4419 4565 387
Size: 1064 138 761 1695 374 146 1280
Fragments arranged by size:
1695 1280 1064 761 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2712 2725 3521 4118 4905 5173 1239
Size: 393 1080 13 796 597 787 268 1524
Fragments arranged by size:
1524 1080 796 787 597 393 268 13
EcoRI G'AATT_C
Cuts at: 2321 2767 3145 2321
Size: 446 378 4634
Fragments arranged by size:
4634 446 378
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2408 4605 1678
Size: 730 2197 2531
Fragments arranged by size:
2531 2197 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 4099 4131 4173
Size: 32 135 32 436 32 2059 32 42
Cuts at: 4173 4205 1373
Size: 32 2626
Fragments arranged by size:
2626 2059 436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2072 2630 4572 4756 1305
Size: 767 558 1942 184 2007
Fragments arranged by size:
2007 1942 767 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 3416 3775 3934 518
Size: 648 1174 1076 359 159 2042
Fragments arranged by size:
2042 1174 1076 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 2874 3551 4699 4944 1606
Size: 636 632 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 632 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NsiI A_TGCA'T
Cuts at: 2542 2542
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PfoI T'CCnGG_A
Cuts at: 2282 2689 2282
Size: 407 5051
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2099 3527 3559 5040 5072 2067
Size: 32 1428 32 1481 32 2453
Fragments arranged by size:
2453 1481 1428 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2414 2810 830
Size: 1584 396 3478
Fragments arranged by size:
3478 1584 396
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
PvuII CAG'CTG
Cuts at: 2242 2242
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SexAI A'CCwGG_T
Cuts at: 2270 2630 2270
Size: 360 5098
SfcI C'TryA_G
Cuts at: 826 1045 2410 2667 2806 3941 4622 4813 826
Size: 219 1365 257 139 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 681 257 219 191 139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2513 2897 513
Size: 552 1448 384 3074
Fragments arranged by size:
3074 1448 552 384
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 3704 5416
Size: 274 80 33 51 1443 510 1217 1712
Cuts at: 5416 96
Size: 138
Fragments arranged by size:
1712 1443 1217 510 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 5418 5450 2421
Size: 32 2965 32 2429
Fragments arranged by size:
2965 2429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI