Restriction map for
pFN21AE2759
(Circular) (Six-base) MAPSORT of: pFN21AE2759.seq Check: 5724 from: 1 to: 7051
pFN21AE2759 7051 bp
With 182 enzymes: SgfI *
September 1, 2019 16:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3112 3552 3793 3112
Size: 440 241 6370
Fragments arranged by size:
6370 440 241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4861 278
Size: 53 83 186 936 3325 2468
Fragments arranged by size:
3325 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3063 3888 3936 4394 1937
Size: 1126 825 48 458 4594
Fragments arranged by size:
4594 1126 825 458 48
Acc65I G'GTAC_C
Cuts at: 2739 4372 2739
Size: 1633 5418
AclI AA'CG_TT
Cuts at: 1563 5178 5551 1563
Size: 3615 373 3063
Fragments arranged by size:
3615 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2918 3073 3230 3476 3592 3688 4312
Size: 872 414 155 157 246 116 96 624
Cuts at: 4312 5114 6498 6766 1632
Size: 802 1384 268 1917
Fragments arranged by size:
1917 1384 872 802 624 414 268 246
157 155 116 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6854
AflIII A'CryG_T
Cuts at: 1203 2511 2901 5950 6927 1203
Size: 1308 390 3049 977 1327
Fragments arranged by size:
3049 1327 1308 977 390
AgeI A'CCGG_T
Cuts at: 5882 5882
Size: 7051
AhdI GACnn_n'nnGTC
Cuts at: 5780 5780
Size: 7051
AleI CACnn'nnGTG
Cuts at: 1166 2807 3134 4346 1166
Size: 1641 327 1212 3871
Fragments arranged by size:
3871 1641 1212 327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7051
AlwNI CAG_nnn'CTG
Cuts at: 2079 2173 3411 3474 3570 3808 4231 4264 4794
Size: 94 1238 63 96 238 423 33 530
Cuts at: 4794 6366 2079
Size: 1572 2764
Fragments arranged by size:
2764 1572 1238 530 423 238 96 94
63 33
ApaLI G'TGCA_C
Cuts at: 3158 5108 6264 3158
Size: 1950 1156 3945
Fragments arranged by size:
3945 1950 1156
ApoI r'AATT_y
Cuts at: 1493 4360 4586 4738 6847 1493
Size: 2867 226 152 2109 1697
Fragments arranged by size:
2867 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5605 160
Size: 872 4573 1606
Fragments arranged by size:
4573 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 3549 3557 3879 4376 1870
Size: 73 1410 196 8 322 497 4545
Fragments arranged by size:
4545 1410 497 322 196 73 8
BamHI G'GATC_C
Cuts at: 2028 2241 4381 4748 2028
Size: 213 2140 367 4331
Fragments arranged by size:
4331 2140 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 2739 3540 3685 3759
Size: 325 217 627 821 131 801 145 74
Cuts at: 3759 3766 4372 5827 618
Size: 7 606 1455 1842
Fragments arranged by size:
1842 1455 821 801 627 606 325 217
145 131 74 7
BanII G_rGCy'C
Cuts at: 1391 2043 2184 3154 3564 4019 4370 1391
Size: 652 141 970 410 455 351 4072
Fragments arranged by size:
4072 970 652 455 410 351 141
BbeI G_GCGC'C
Cuts at: 3689 3763 3689
Size: 74 6977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2495 3967 928
Size: 971 325 271 1472 4012
Fragments arranged by size:
4012 1472 971 325 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3460 3494 5242 5276 1903
Size: 34 50 34 1439 34 1748 34 3678
Fragments arranged by size:
3678 1748 1439 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4943 6153 4943
Size: 1210 5841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7051
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2299 2321 3375
Size: 107 122 71 631 605 627 22 1054
Cuts at: 3375 3948 5662 136
Size: 573 1714 1525
Fragments arranged by size:
1714 1525 1054 631 627 605 573 122
107 71 22
BglII A'GATC_T
Cuts at: 2685 2853 7046 2685
Size: 168 4193 2690
Fragments arranged by size:
4193 2690 168
BlpI GC'TnA_GC
Cuts at: 3467 4468 3467
Size: 1001 6050
Bme1580I G_kGCm'C
Cuts at: 1790 3108 3162 3543 4179 5112 6268 1790
Size: 1318 54 381 636 933 1156 2573
Fragments arranged by size:
2573 1318 1156 933 636 381 54
BmgBI CAC'GTC
Cuts at: 1318 2493 3052 4348 1318
Size: 1175 559 1296 4021
Fragments arranged by size:
4021 1296 1175 559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2899 4782 5740
Size: 416 53 83 399 24 1482 1883 958
Cuts at: 5740 442
Size: 1753
Fragments arranged by size:
1883 1753 1482 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2309 2870 3134 3830 4232 5711 1239
Size: 956 114 561 264 696 402 1479 2579
Fragments arranged by size:
2579 1479 956 696 561 402 264 114
Bpu10I CC'TnA_GC
Cuts at: 2183 3563 2183
Size: 1380 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3929 5176 6041 6339 3929
Size: 1247 865 298 4641
Fragments arranged by size:
4641 1247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2136 2405 3806 5714 882
Size: 617 579 58 269 1401 1908 2219
Fragments arranged by size:
2219 1908 1401 617 579 269 58
BsaAI yAC'GTr
Cuts at: 493 1204 2902 4138 4842 493
Size: 711 1698 1236 704 2702
Fragments arranged by size:
2702 1698 1236 711 704
BsaBI GATnn'nnATC
Cuts at: 2069 3440 3638 7045 2069
Size: 1371 198 3407 2075
Fragments arranged by size:
3407 2075 1371 198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2479 3686 3732 3760
Size: 53 83 186 936 946 1207 46 28
Cuts at: 3760 4858 5240 5875 275
Size: 1098 382 635 1451
Fragments arranged by size:
1451 1207 1098 946 936 635 382 186
83 53 46 28
BsaWI w'CCGG_w
Cuts at: 2025 5484 5882 6156 6303 6714 2025
Size: 3459 398 274 147 411 2362
Fragments arranged by size:
3459 2362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 4061 4091 4205
Size: 30 165 30 1560 30 1068 30 114
Cuts at: 4205 4235 1178
Size: 30 3994
Fragments arranged by size:
3994 1560 1068 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2273 3131 1186
Size: 1006 81 858 5106
Fragments arranged by size:
5106 1006 858 81
BseYI C'CCAG_C
Cuts at: 2584 4264 4315 6254 6750 2584
Size: 1680 51 1939 496 2885
Fragments arranged by size:
2885 1939 1680 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 2746 2870 3452 4057 1163
Size: 573 711 299 124 582 605 4157
Fragments arranged by size:
4157 711 605 582 573 299 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2616 3102 3135 4501 5262 6290
Size: 275 186 615 486 33 1366 761 1028
Cuts at: 6290 1540
Size: 2301
Fragments arranged by size:
2301 1366 1028 761 615 486 275 186
33
BsiHKAI G_wGCw'C
Cuts at: 2184 3162 3564 4137 4343 5112 5197 6268 2184
Size: 978 402 573 206 769 85 1071 2967
Fragments arranged by size:
2967 1071 978 769 573 402 206 85
BsmI GAATG_Cn'
Cuts at: 4562 4655 4562
Size: 93 6958
BsmBI CGTCTCn'nnnn_
Cuts at: 3312 3312
Size: 7051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2184 3108 3154 3162 3543 3564
Size: 399 253 141 924 46 8 381 21
Cuts at: 3564 4019 4137 4179 4343 4370 5112 5197 6268
Size: 455 118 42 164 27 742 85 1071
Cuts at: 6268 1391
Size: 2174
Fragments arranged by size:
2174 1071 924 742 455 399 381 253
164 141 118 85 46 42 27 21
8
BspEI T'CCGG_A
Cuts at: 2025 6714 2025
Size: 4689 2362
BspHI T'CATG_A
Cuts at: 2385 3021 3033 3180 4940 6670 2385
Size: 636 12 147 1760 1730 2766
Fragments arranged by size:
2766 1760 1730 636 147 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2768 3112 3249 3552 3793 4406 844
Size: 1883 41 344 137 303 241 613 3489
Fragments arranged by size:
3489 1883 613 344 303 241 137 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2646 3516 4938 1394
Size: 429 823 870 1422 3507
Fragments arranged by size:
3507 1422 870 823 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3834 3852 5546 5720 65
Size: 1183 2586 18 1694 174 1396
Fragments arranged by size:
2586 1694 1396 1183 174 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 2611 5695 5882 1700
Size: 110 801 3084 187 2869
Fragments arranged by size:
3084 2869 801 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7051
BssHII G'CGCG_C
Cuts at: 1925 2869 1925
Size: 944 6107
BssSI C'ACGA_G
Cuts at: 2117 2350 3713 5111 6123 2117
Size: 233 1363 1398 1012 3045
Fragments arranged by size:
3045 1398 1363 1012 233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7051
BstBI TT'CG_AA
Cuts at: 5855 5855
Size: 7051
BstEII G'GTnAC_C
Cuts at: 1184 2344 3124 3142 3499 1184
Size: 1160 780 18 357 4736
Fragments arranged by size:
4736 1160 780 357 18
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 3136 3370 3448 3667 3742 3847 4111 2824
Size: 312 234 78 219 75 105 264 5764
Fragments arranged by size:
5764 312 264 234 219 105 78 75
BstYI r'GATC_y
Cuts at: 1972 2028 2241 2422 2685 2853 3720 4381 4748
Size: 56 213 181 263 168 867 661 367
Cuts at: 4748 5133 5150 6602 6688 6700 7046 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 867 661 385 367 346 263
213 181 168 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2295 2295
Size: 7051
BtgI C'CryG_G
Cuts at: 513 1065 2838 3129 3135 3494 513
Size: 552 1773 291 6 359 4070
Fragments arranged by size:
4070 1773 552 359 291 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3304 4144 487
Size: 683 56 2078 840 3394
Fragments arranged by size:
3394 2078 840 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 3281 4567 5361 5381 2108
Size: 1173 1286 794 20 3778
Fragments arranged by size:
3778 1286 1173 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7016
DraI TTT'AAA
Cuts at: 4355 4355
Size: 7051
DraIII CAC_nnn'GTG
Cuts at: 3166 3166
Size: 7051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 6058 809
Size: 507 942 3800 1802
Fragments arranged by size:
3800 1802 942 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2613 2966
Size: 54 1425 50 275 18 492 291 353
Cuts at: 2966 3099 3132 3346 3438 3669 4101 4498 5387
Size: 133 33 214 92 231 432 397 889
Cuts at: 5387 7016 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 492 432 397 353 291
275 231 214 133 92 54 50 43
33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2613 3099 3132 4498 1537
Size: 275 801 486 33 1366 4090
Fragments arranged by size:
4090 1366 801 486 275 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 3092 3387 3431 3707 4981 1353
Size: 657 1082 295 44 276 1274 3423
Fragments arranged by size:
3423 1274 1082 657 295 276 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3713 4097 5638 6012 6158 387
Size: 1064 138 2124 384 1541 374 146 1280
Fragments arranged by size:
2124 1541 1280 1064 384 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 3562 2182
Size: 1380 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2309 2504 2870 2918 3073 3134
Size: 393 563 114 195 366 48 155 61
Cuts at: 3134 3230 3476 3592 3688 3830 4232 4312 5114
Size: 96 246 116 96 142 402 80 802
Cuts at: 5114 5711 6498 6766 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 802 787 597 563 402 393 366
268 246 195 155 142 116 114 96
96 80 61 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2465
Size: 7051
EcoO109I rG'GnC_Cy
Cuts at: 2300 2893 3237 3553 3792 3997 2300
Size: 593 344 316 239 205 5354
Fragments arranged by size:
5354 593 344 316 239 205
EcoRI G'AATT_C
Cuts at: 4360 4738 4360
Size: 378 6673
FspI TGC'GCA
Cuts at: 5557 5557
Size: 7051
HaeII r_GCGC'y
Cuts at: 1678 3689 3763 6198 1678
Size: 2011 74 2435 2531
Fragments arranged by size:
2531 2435 2011 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3136 3168 5692 5724 5766
Size: 32 135 32 1564 32 2524 32 42
Cuts at: 5766 5798 1373
Size: 32 2626
Fragments arranged by size:
2626 2524 1564 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3601 3937 3958 4395 4641 669
Size: 988 281 1663 336 21 437 246 3079
Fragments arranged by size:
3079 1663 988 437 336 281 246 21
HindIII A'AGCT_T
Cuts at: 748 3212 748
Size: 2464 4587
HpaI GTT'AAC
Cuts at: 4641 4641
Size: 7051
KasI G'GCGC_C
Cuts at: 3685 3759 3685
Size: 74 6977
KpnI G_GTAC'C
Cuts at: 2743 4376 2743
Size: 1633 5418
MfeI C'AATT_G
Cuts at: 4650 4650
Size: 7051
MluI A'CGCG_T
Cuts at: 6927 6927
Size: 7051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6165 6349 1305
Size: 4860 184 2007
Fragments arranged by size:
4860 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3440 4103 10
Size: 54 1425 343 1608 663 2958
Fragments arranged by size:
2958 1608 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2600 2606 2708 2807 2822 3134
Size: 648 1041 393 6 102 99 15 312
Cuts at: 3134 3179 3446 3632 3665 3740 3821 4346 5009
Size: 45 267 186 33 75 81 525 663
Cuts at: 5009 5368 5527 518
Size: 359 159 2042
Fragments arranged by size:
2042 1041 663 648 525 393 359 312
267 186 159 102 99 81 75 45
33 15 6
MspA1I CmG'CkG
Cuts at: 1606 2062 2746 3408 3682 3691 3757 4467 5144
Size: 456 684 662 274 9 66 710 677
Cuts at: 5144 6292 6537 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 710 684 677 662 456 274
245 66 9
NaeI GCC'GGC
Cuts at: 2613 2613
Size: 7051
NarI GG'CG_CC
Cuts at: 3686 3760 3686
Size: 74 6977
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6499
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7051
NgoMIV G'CCGG_C
Cuts at: 2611 2611
Size: 7051
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7051
NotI GC'GGCC_GC
Cuts at: 4498 4498
Size: 7051
NspI r_CATG'y
Cuts at: 1132 1229 2515 2605 4409 5954 1132
Size: 97 1286 90 1804 1545 2229
Fragments arranged by size:
2229 1804 1545 1286 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7051
PciI A'CATG_T
Cuts at: 2511 5950 2511
Size: 3439 3612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 1108
Size: 1947 5104
PfoI T'CCnGG_A
Cuts at: 2172 2214 2286 2517 2847 3111 3993 2172
Size: 42 72 231 330 264 882 5230
Fragments arranged by size:
5230 882 330 264 231 72 42
PmeI GTTT'AAAC
Cuts at: 4355 4355
Size: 7051
PmlI CAC'GTG
Cuts at: 4138 4138
Size: 7051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 3245 3277 5120 5152 6633 6665 2438
Size: 32 775 32 1843 32 1481 32 2824
Fragments arranged by size:
2824 1843 1481 775 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2893 3237 3553 3792 3997 2893
Size: 344 316 239 205 5947
Fragments arranged by size:
5947 344 316 239 205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7051
PsiI TTA'TAA
Cuts at: 4621 4621
Size: 7051
PstI C_TGCA'G
Cuts at: 830 3288 3804 4403 830
Size: 2458 516 599 3478
Fragments arranged by size:
3478 2458 599 516
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7051
SacI G_AGCT'C
Cuts at: 2184 3564 2184
Size: 1380 5671
SalI G'TCGA_C
Cuts at: 1936 3935 4393 1936
Size: 1999 458 4594
Fragments arranged by size:
4594 1999 458
SanDI GG'GwC_CC
Cuts at: 3553 3553
Size: 7051
SapI GCTCTTCn'nnn_
Cuts at: 3092 3707 3092
Size: 615 6436
SbfI CC_TGCA'GG
Cuts at: 4403 4403
Size: 7051
ScaI AGT'ACT
Cuts at: 5299 7011 5299
Size: 1712 5339
SexAI A'CCwGG_T
Cuts at: 3486 3486
Size: 7051
SfcI C'TryA_G
Cuts at: 826 1045 3284 3800 3901 4399 5534 6215 6406
Size: 219 2239 516 101 498 1135 681 191
Cuts at: 6406 826
Size: 1471
Fragments arranged by size:
2239 1471 1135 681 516 498 219 191
101
SfoI GGC'GCC
Cuts at: 3687 3761 3687
Size: 74 6977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7051
SmaI CCC'GGG
Cuts at: 1872 3355 3551 4378 1872
Size: 1483 196 827 4545
Fragments arranged by size:
4545 1483 827 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3944 5155 6056 6318 820
Size: 197 926 2001 1211 901 262 1553
Fragments arranged by size:
2001 1553 1211 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4842 493
Size: 4349 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7051
SphI G_CATG'C
Cuts at: 1132 4409 1132
Size: 3277 3774
SspI AAT'ATT
Cuts at: 5 52 4975 5
Size: 47 4923 2081
Fragments arranged by size:
4923 2081 47
StuI AGG'CCT
Cuts at: 1517 2093 1517
Size: 576 6475
StyI C'CwwG_G
Cuts at: 513 1065 3780 3984 4490 513
Size: 552 2715 204 506 3074
Fragments arranged by size:
3074 2715 552 506 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2150 2795 3311 3487 4256 5078
Size: 1255 522 48 645 516 176 769 822
Cuts at: 5078 5095 5248 5433 5878 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 822 769 645 522 516 445
185 176 153 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 5297 7009
Size: 274 80 33 51 1443 594 2726 1712
Cuts at: 7009 96
Size: 138
Fragments arranged by size:
2726 1712 1443 594 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3953 3985 7011 7043 3953
Size: 32 3026 32 3961
Fragments arranged by size:
3961 3026 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 2438 1316
Size: 795 327 5929
Fragments arranged by size:
5929 795 327
XbaI T'CTAG_A
Cuts at: 4387 4387
Size: 7051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 3136 3664 3823 4001 2845
Size: 291 528 159 178 5895
Fragments arranged by size:
5895 528 291 178 159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7051
XmaI C'CCGG_G
Cuts at: 1870 3353 3549 4376 1870
Size: 1483 196 827 4545
Fragments arranged by size:
4545 1483 827 196
XmnI GAAnn'nnTTC
Cuts at: 5180 5180
Size: 7051
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4859 276
Size: 53 83 186 936 3325 2468
Fragments arranged by size:
3325 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII
SfiI SrfI SwaI