Restriction map for
pFN21AE2748
(Circular) (Six-base) MAPSORT of: pFN21AE2748.seq Check: 8663 from: 1 to: 12100
pFN21AE2748 12100 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 8074 8074
Size: 12100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9910 278
Size: 53 83 186 936 8374 2468
Fragments arranged by size:
8374 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2215 2434 7251 9031 9443 1937
Size: 278 219 4817 1780 412 4594
Fragments arranged by size:
4817 4594 1780 412 278 219
Acc65I G'GTAC_C
Cuts at: 9421 9421
Size: 12100
AclI AA'CG_TT
Cuts at: 1563 5056 10227 10600 1563
Size: 3493 5171 373 3063
Fragments arranged by size:
5171 3493 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 2961 3181 3350 3774 3963 5306 5962
Size: 1056 273 220 169 424 189 1343 656
Cuts at: 5962 6245 6746 6825 6866 7341 7694 7889 8790
Size: 283 501 79 41 475 353 195 901
Cuts at: 8790 10163 11547 11815 1632
Size: 1373 1384 268 1917
Fragments arranged by size:
1917 1384 1373 1343 1056 901 656 501
475 424 353 283 273 268 220 195
189 169 79 41
AflII C'TTAA_G
Cuts at: 820 1017 4525 6752 820
Size: 197 3508 2227 6168
Fragments arranged by size:
6168 3508 2227 197
AflIII A'CryG_T
Cuts at: 1203 6106 6908 10999 11976 1203
Size: 4903 802 4091 977 1327
Fragments arranged by size:
4903 4091 1327 977 802
AgeI A'CCGG_T
Cuts at: 10931 10931
Size: 12100
AhdI GACnn_n'nnGTC
Cuts at: 10829 10829
Size: 12100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4045 5136 6752 1184
Size: 2861 1091 1616 6532
Fragments arranged by size:
6532 2861 1616 1091
AlwNI CAG_nnn'CTG
Cuts at: 3532 3609 4384 5653 6769 7622 9193 9843 11415
Size: 77 775 1269 1116 853 1571 650 1572
Cuts at: 11415 3532
Size: 4217
Fragments arranged by size:
4217 1572 1571 1269 1116 853 775 650
77
ApaI G_GGCC'C
Cuts at: 7454 8814 7454
Size: 1360 10740
ApaLI G'TGCA_C
Cuts at: 8244 10157 11313 8244
Size: 1913 1156 9031
Fragments arranged by size:
9031 1913 1156
ApoI r'AATT_y
Cuts at: 1493 2083 4174 4610 5116 6366 6477 6716 7069
Size: 590 2091 436 506 1250 111 239 353
Cuts at: 7069 7737 8912 9044 9409 9635 9787 11896 1493
Size: 668 1175 132 365 226 152 2109 1697
Fragments arranged by size:
2109 2091 1697 1250 1175 668 590 506
436 365 353 239 226 152 132 111
AseI AT'TA_AT
Cuts at: 160 1032 6699 7401 10654 160
Size: 872 5667 702 3253 1606
Fragments arranged by size:
5667 3253 1606 872 702
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12100
AvaI C'yCGr_G
Cuts at: 1870 1943 5335 9425 1870
Size: 73 3392 4090 4545
Fragments arranged by size:
4545 4090 3392 73
BamHI G'GATC_C
Cuts at: 7213 8353 9430 9797 7213
Size: 1140 1077 367 9516
Fragments arranged by size:
9516 1140 1077 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 8641 9421 10876 618
Size: 325 217 627 6854 780 1455 1842
Fragments arranged by size:
6854 1842 1455 780 627 325 217
BanII G_rGCy'C
Cuts at: 1391 7454 8814 9419 1391
Size: 6063 1360 605 4072
Fragments arranged by size:
6063 4072 1360 605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 2869 3720 4010 4610 7522 7802
Size: 971 662 308 851 290 600 2912 280
Cuts at: 7802 9117 9214 928
Size: 1315 97 3814
Fragments arranged by size:
3814 2912 1315 971 851 662 600 308
290 280 97
BbvCI CC'TCA_GC
Cuts at: 4299 4665 4299
Size: 366 11734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10291 10325 1903
Size: 34 8354 34 3678
Fragments arranged by size:
8354 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9992 11202 9992
Size: 1210 10890
BclI T'GATC_A
Cuts at: 1548 2802 7305 1548
Size: 1254 4503 6343
Fragments arranged by size:
6343 4503 1254
BfrBI ATG'CAT
Cuts at: 2873 6236 8114 8212 2873
Size: 3363 1878 98 6761
Fragments arranged by size:
6761 3363 1878 98
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10711 136
Size: 107 122 71 631 605 9039 1525
Fragments arranged by size:
9039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2308 4828 12095 2308
Size: 2520 7267 2313
Fragments arranged by size:
7267 2520 2313
BlpI GC'TnA_GC
Cuts at: 6523 6897 8285 9517 6523
Size: 374 1388 1232 9106
Fragments arranged by size:
9106 1388 1232 374
Bme1580I G_kGCm'C
Cuts at: 1790 2557 7454 7749 8226 8248 8814 9150 10161
Size: 767 4897 295 477 22 566 336 1011
Cuts at: 10161 11317 1790
Size: 1156 2573
Fragments arranged by size:
4897 2573 1156 1011 767 566 477 336
295 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3520 4280 4497
Size: 416 53 83 399 24 2103 760 217
Cuts at: 4497 8352 9831 10789 442
Size: 3855 1479 958 1753
Fragments arranged by size:
3855 2103 1753 1479 958 760 416 399
217 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3657 1056
Size: 2601 9499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2080 3399 3551 3681 4361 5686 6042
Size: 803 38 1319 152 130 680 1325 356
Cuts at: 6042 6107 7803 8849 9332 10760 1239
Size: 65 1696 1046 483 1428 2579
Fragments arranged by size:
2579 1696 1428 1325 1319 1046 803 680
483 356 152 130 65 38
Bpu10I CC'TnA_GC
Cuts at: 2292 2837 3096 3623 4299 4665 2292
Size: 545 259 527 676 366 9727
Fragments arranged by size:
9727 676 545 527 366 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2701 4261 5389 7444 8643 10225 11090 11388 2701
Size: 1560 1128 2055 1199 1582 865 298 3413
Fragments arranged by size:
3413 2055 1582 1560 1199 1128 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7893 10763 882
Size: 617 6394 2870 2219
Fragments arranged by size:
6394 2870 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2607 9058 9891 493
Size: 711 1403 6451 833 2702
Fragments arranged by size:
6451 2702 1403 833 711
BsaBI GATnn'nnATC
Cuts at: 2807 5292 5565 12094 2807
Size: 2485 273 6529 2813
Fragments arranged by size:
6529 2813 2485 273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7769 9907 10289 10924
Size: 53 83 186 936 6236 2138 382 635
Cuts at: 10924 275
Size: 1451
Fragments arranged by size:
6236 2138 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2794 6282 6345 10533 10931 11205 11352 11763 2794
Size: 3488 63 4188 398 274 147 411 3131
Fragments arranged by size:
4188 3488 3131 411 398 274 147 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4359 4389 5229 5259 5785
Size: 30 165 30 2956 30 840 30 526
Cuts at: 5785 5815 6096 6126 8822 8852 1178
Size: 30 281 30 2696 30 4426
Fragments arranged by size:
4426 2956 2696 840 526 281 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5105 5704 6234 1186
Size: 3919 599 530 7052
Fragments arranged by size:
7052 3919 599 530
BseYI C'CCAG_C
Cuts at: 5357 7764 11303 11799 5357
Size: 2407 3539 496 5658
Fragments arranged by size:
5658 3539 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2729 4379 4500 5575 6414 6951 7084
Size: 573 993 1650 121 1075 839 537 133
Cuts at: 7084 9151 1163
Size: 2067 4112
Fragments arranged by size:
4112 2067 1650 1075 993 839 573 537
133 121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2795 9550 10311 11339 1540
Size: 275 186 794 6755 761 1028 2301
Fragments arranged by size:
6755 2301 1028 794 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4277 6114 6622 8248 9213 10161 10246 11317 4277
Size: 1837 508 1626 965 948 85 1071 5060
Fragments arranged by size:
5060 1837 1626 1071 965 948 508 85
BsmI GAATG_Cn'
Cuts at: 2543 4396 5404 5626 9611 9704 2543
Size: 1853 1008 222 3985 93 4939
Fragments arranged by size:
4939 3985 1853 1008 222 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5573 5573
Size: 12100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2557 4277 6114 6622 7454 7749 8226
Size: 399 767 1720 1837 508 832 295 477
Cuts at: 8226 8248 8814 9150 9213 9419 10161 10246 11317
Size: 22 566 336 63 206 742 85 1071
Cuts at: 11317 1391
Size: 2174
Fragments arranged by size:
2174 1837 1720 1071 832 767 742 566
508 477 399 336 295 206 85 63
22
BspEI T'CCGG_A
Cuts at: 6345 11763 6345
Size: 5418 6682
BspHI T'CATG_A
Cuts at: 3739 5749 7608 9989 11719 3739
Size: 2010 1859 2381 1730 4120
Fragments arranged by size:
4120 2381 2010 1859 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6638 8074 9455 844
Size: 5794 1436 1381 3489
Fragments arranged by size:
5794 3489 1436 1381
BsrBI CCG'CTC
Cuts at: 1394 1823 8182 9987 1394
Size: 429 6359 1805 3507
Fragments arranged by size:
6359 3507 1805 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2375 3003 3572 4029 4293 4991 5137
Size: 1183 1127 628 569 457 264 698 146
Cuts at: 5137 7491 8076 8933 10595 10769 65
Size: 2354 585 857 1662 174 1396
Fragments arranged by size:
2354 1662 1396 1183 1127 857 698 628
585 569 457 264 174 146
BsrFI r'CCGG_y
Cuts at: 1700 1810 10744 10931 1700
Size: 110 8934 187 2869
Fragments arranged by size:
8934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2728 5044 6357 8746 96
Size: 2632 2316 1313 2389 3450
Fragments arranged by size:
3450 2632 2389 2316 1313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12100
BssSI C'ACGA_G
Cuts at: 4977 8457 10160 11172 4977
Size: 3480 1703 1012 5905
Fragments arranged by size:
5905 3480 1703 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 5110 7940 8252 9154 9193 794
Size: 1560 2756 2830 312 902 39 3701
Fragments arranged by size:
3701 2830 2756 1560 902 312 39
BstBI TT'CG_AA
Cuts at: 3291 10904 3291
Size: 7613 4487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12100
BstXI CCAn_nnnn'nTGG
Cuts at: 7756 8017 7756
Size: 261 11839
BstYI r'GATC_y
Cuts at: 1972 2308 2808 3052 3175 3618 3938 4132 4828
Size: 336 500 244 123 443 320 194 696
Cuts at: 4828 5566 6062 7213 8353 9430 9797 10182 10199
Size: 738 496 1151 1140 1077 367 385 17
Cuts at: 10199 11651 11737 11749 12095 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1151 1140 1077 738 696 500
496 443 385 367 346 336 320 244
194 123 86 17 12
BstZ17I GTA'TAC
Cuts at: 9032 9032
Size: 12100
BtgI C'CryG_G
Cuts at: 513 1065 4479 5187 513
Size: 552 3414 708 7426
Fragments arranged by size:
7426 3414 708 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11361
Fragments arranged by size:
11361 683 56
BtsI GCAGTG_nn'
Cuts at: 2674 3350 4034 5266 5563 6939 7285 7322 8472
Size: 676 684 1232 297 1376 346 37 1150
Cuts at: 8472 8572 8778 9616 10410 10430 2674
Size: 100 206 838 794 20 4344
Fragments arranged by size:
4344 1376 1232 1150 838 794 684 676
346 297 206 100 37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12065
DraI TTT'AAA
Cuts at: 2625 7650 9049 9404 2625
Size: 5025 1399 355 5321
Fragments arranged by size:
5321 5025 1399 355
DraIII CAC_nnn'GTG
Cuts at: 7280 7280
Size: 12100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5793 11107 809
Size: 507 4477 5314 1802
Fragments arranged by size:
5314 4477 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4476 6273 9547
Size: 54 1425 50 275 18 2646 1797 3274
Cuts at: 9547 10436 12065 8
Size: 889 1629 43
Fragments arranged by size:
3274 2646 1797 1629 1425 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9547 1537
Size: 275 7735 4090
Fragments arranged by size:
7735 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2346 2762 3027 4268 4537 4673 5571
Size: 657 336 416 265 1241 269 136 898
Cuts at: 5571 5751 5761 6497 6801 6885 9351 10030 1353
Size: 180 10 736 304 84 2466 679 3423
Fragments arranged by size:
3423 2466 1241 898 736 679 657 416
336 304 269 265 180 136 84 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10687 11061 11207 387
Size: 1064 138 9098 374 146 1280
Fragments arranged by size:
9098 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2080 2688 2961 3181 3350 3399
Size: 393 410 38 608 273 220 169 49
Cuts at: 3399 3551 3681 3774 3963 4361 5306 5686 5962
Size: 152 130 93 189 398 945 380 276
Cuts at: 5962 6042 6107 6245 6746 6825 6866 7341 7694
Size: 80 65 138 501 79 41 475 353
Cuts at: 7694 7803 7889 8790 8849 9332 10163 10760 11547
Size: 109 86 901 59 483 831 597 787
Cuts at: 11547 11815 1239
Size: 268 1524
Fragments arranged by size:
1524 945 901 831 787 608 597 501
483 475 410 398 393 380 353 276
273 268 220 189 169 152 138 130
109 93 86 80 79 65 59 49
41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3099 3626 4574 3099
Size: 527 948 10625
Fragments arranged by size:
10625 948 527
EcoO109I rG'GnC_Cy
Cuts at: 2912 3104 7451 8810 8811 2912
Size: 192 4347 1359 1 6201
Fragments arranged by size:
6201 4347 1359 192 1
EcoRI G'AATT_C
Cuts at: 7737 9409 9787 7737
Size: 1672 378 10050
Fragments arranged by size:
10050 1672 378
EcoRV GAT'ATC
Cuts at: 2118 2118
Size: 12100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2669 4151 5924 6605 7589 2669
Size: 1482 1773 681 984 7180
Fragments arranged by size:
7180 1773 1482 984 681
FspI TGC'GCA
Cuts at: 9176 10606 9176
Size: 1430 10670
HaeII r_GCGC'y
Cuts at: 1678 2255 11247 1678
Size: 577 8992 2531
Fragments arranged by size:
8992 2531 577
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2386 2418 3547
Size: 32 135 32 530 32 252 32 1129
Cuts at: 3547 3579 4554 4586 5229 5261 6545 6577 7153
Size: 32 975 32 643 32 1284 32 576
Cuts at: 7153 7185 8045 8077 8259 8291 10741 10773 10815
Size: 32 860 32 182 32 2450 32 42
Cuts at: 10815 10847 1373
Size: 32 2626
Fragments arranged by size:
2626 2450 1284 1129 975 860 643 576
530 252 182 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4758 9444 9690 669
Size: 988 281 2820 4686 246 3079
Fragments arranged by size:
4686 3079 2820 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2633 8334 748
Size: 1885 5701 4514
Fragments arranged by size:
5701 4514 1885
HpaI GTT'AAC
Cuts at: 4758 9690 4758
Size: 4932 7168
KpnI G_GTAC'C
Cuts at: 9425 9425
Size: 12100
MfeI C'AATT_G
Cuts at: 2064 3894 7935 7944 9699 2064
Size: 1830 4041 9 1755 4465
Fragments arranged by size:
4465 4041 1830 1755 9
MluI A'CGCG_T
Cuts at: 11976 11976
Size: 12100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 3153 3423 4072 7191 7241 11214 11398
Size: 1185 663 270 649 3119 50 3973 184
Cuts at: 11398 1305
Size: 2007
Fragments arranged by size:
3973 3119 2007 1185 663 649 270 184
50
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4478 6275 10
Size: 54 1425 343 2646 1797 5835
Fragments arranged by size:
5835 2646 1797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 3044 5682 6323 6461 7460 7501
Size: 648 1197 681 2638 641 138 999 41
Cuts at: 7501 10058 10417 10576 518
Size: 2557 359 159 2042
Fragments arranged by size:
2638 2557 2042 1197 999 681 648 641
359 159 138 41
MspA1I CmG'CkG
Cuts at: 1606 3409 3884 8281 8881 9516 10193 11341 11586
Size: 1803 475 4397 600 635 677 1148 245
Cuts at: 11586 1606
Size: 2120
Fragments arranged by size:
4397 2120 1803 1148 677 635 600 475
245
NcoI C'CATG_G
Cuts at: 513 1065 4479 5187 513
Size: 552 3414 708 7426
Fragments arranged by size:
7426 3414 708 552
NdeI CA'TA_TG
Cuts at: 387 2263 7302 387
Size: 1876 5039 5185
Fragments arranged by size:
5185 5039 1876
NheI G'CTAG_C
Cuts at: 1052 3653 1052
Size: 2601 9499
NotI GC'GGCC_GC
Cuts at: 9547 9547
Size: 12100
NsiI A_TGCA'T
Cuts at: 2875 6238 8116 8214 2875
Size: 3363 1878 98 6761
Fragments arranged by size:
6761 3363 1878 98
NspI r_CATG'y
Cuts at: 1132 1229 2951 3359 4358 4625 4796 6110 6397
Size: 97 1722 408 999 267 171 1314 287
Cuts at: 6397 6460 6896 6912 6922 6966 7708 9458 11003
Size: 63 436 16 10 44 742 1750 1545
Cuts at: 11003 1132
Size: 2229
Fragments arranged by size:
2229 1750 1722 1545 1314 999 742 436
408 287 267 171 97 63 44 16
10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12100
PciI A'CATG_T
Cuts at: 6106 6908 10999 6106
Size: 802 4091 7207
Fragments arranged by size:
7207 4091 802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2103 2998 7240 1108
Size: 995 895 4242 5968
Fragments arranged by size:
5968 4242 995 895
PfoI T'CCnGG_A
Cuts at: 5083 5702 6058 6140 5083
Size: 619 356 82 11043
Fragments arranged by size:
11043 619 356 82
PmeI GTTT'AAAC
Cuts at: 9404 9404
Size: 12100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10169 10201 11682 11714 10169
Size: 32 1481 32 10555
Fragments arranged by size:
10555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2912 3104 2912
Size: 192 11908
PshAI GACnn'nnGTC
Cuts at: 1613 7544 1613
Size: 5931 6169
PsiI TTA'TAA
Cuts at: 4745 9670 4745
Size: 4925 7175
PspOMI G'GGCC_C
Cuts at: 7450 8810 7450
Size: 1360 10740
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5626 5658 5626
Size: 32 12068
PstI C_TGCA'G
Cuts at: 830 5129 5668 7032 7558 9452 830
Size: 4299 539 1364 526 1894 3478
Fragments arranged by size:
4299 3478 1894 1364 539 526
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12100
PvuII CAG'CTG
Cuts at: 3409 3884 8281 3409
Size: 475 4397 7228
Fragments arranged by size:
7228 4397 475
SalI G'TCGA_C
Cuts at: 1936 9442 1936
Size: 7506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2010 4268 2010
Size: 2258 9842
SbfI CC_TGCA'GG
Cuts at: 9452 9452
Size: 12100
ScaI AGT'ACT
Cuts at: 2486 2701 10348 12060 2486
Size: 215 7647 1712 2526
Fragments arranged by size:
7647 2526 1712 215
SexAI A'CCwGG_T
Cuts at: 2230 2428 4452 6657 2230
Size: 198 2024 2205 7673
Fragments arranged by size:
7673 2205 2024 198
SfcI C'TryA_G
Cuts at: 826 1045 2435 2548 4116 5125 5664 6192 7028
Size: 219 1390 113 1568 1009 539 528 836
Cuts at: 7028 7554 8164 9448 10583 11264 11455 826
Size: 526 610 1284 1135 681 191 1471
Fragments arranged by size:
1568 1471 1390 1284 1135 1009 836 681
610 539 528 526 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12100
SmaI CCC'GGG
Cuts at: 1872 9427 1872
Size: 7555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2716 4276 4525 5368 6752 7423
Size: 197 926 773 1560 249 843 1384 671
Cuts at: 7423 8658 10204 11105 11367 820
Size: 1235 1546 901 262 1553
Fragments arranged by size:
1560 1553 1546 1384 1235 926 901 843
773 671 262 249 197
SnaBI TAC'GTA
Cuts at: 493 9891 493
Size: 9398 2702
SpeI A'CTAG_T
Cuts at: 152 4441 6221 152
Size: 4289 1780 6031
Fragments arranged by size:
6031 4289 1780
SphI G_CATG'C
Cuts at: 1132 6896 7708 9458 1132
Size: 5764 812 1750 3774
Fragments arranged by size:
5764 3774 1750 812
SspI AAT'ATT
Cuts at: 5 52 8206 10024 5
Size: 47 8154 1818 2081
Fragments arranged by size:
8154 2081 1818 47
StuI AGG'CCT
Cuts at: 1517 7126 1517
Size: 5609 6491
StyI C'CwwG_G
Cuts at: 513 1065 4479 5187 5424 7542 7671 9539 513
Size: 552 3414 708 237 2118 129 1868 3074
Fragments arranged by size:
3414 3074 2118 1868 708 552 237 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3852 6990 10127 10144 10297 10482 10927
Size: 1255 2272 3138 3137 17 153 185 445
Cuts at: 10927 325
Size: 1498
Fragments arranged by size:
3138 3137 2272 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 2699 2722
Size: 274 80 33 51 1443 507 215 23
Cuts at: 2722 2728 5035 5044 5143 6357 7092 8307 8746
Size: 6 2307 9 99 1214 735 1215 439
Cuts at: 8746 10346 12058 96
Size: 1600 1712 138
Fragments arranged by size:
2307 1712 1600 1443 1215 1214 735 507
439 274 215 138 99 80 51 33
23 9 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4359 4391 5206 5238 12060 12092 4359
Size: 32 815 32 6822 32 4367
Fragments arranged by size:
6822 4367 815 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4468 1316
Size: 3152 8948
XbaI T'CTAG_A
Cuts at: 3115 5783 6778 9436 3115
Size: 2668 995 2658 5779
Fragments arranged by size:
5779 2668 2658 995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4652 4652
Size: 12100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12100
XmaI C'CCGG_G
Cuts at: 1870 9425 1870
Size: 7555 4545
XmnI GAAnn'nnTTC
Cuts at: 3544 5319 6533 7590 8949 10229 3544
Size: 1775 1214 1057 1359 1280 5415
Fragments arranged by size:
5415 1775 1359 1280 1214 1057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9908 276
Size: 53 83 186 936 8374 2468
Fragments arranged by size:
8374 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI